Abstract:
:First messengers and viral transfection are the two most common ways to stimulate cells for signal output, although their applications are limited. We investigated mechanisms of inducing neural stem cell differentiation using recombinant ephrin-B2/Fc and found that it acted as a ligand and inhibited endogenous ephrin-B2, which maintenance of the neural progenitor cell state, by direct interference. Our results showed the movement of ephrin-B2/Fc within the cell and indicated that it recycled to the plasma membrane surface, revealing a possible pattern of ephrin trafficking. Our results also serve as proof of concept for the reconstruction of the intracellular domain of ephrin using an artificial receptor to direct input signals in future studies.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Xiaodong H,Zhen H,Min S,Zhiming C,Hongyan J,Chong Z,Xuefeng T,Guohua Jdoi
10.1016/j.bbrc.2013.09.070subject
Has Abstractpub_date
2013-10-18 00:00:00pages
300-5issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(13)01551-9journal_volume
440pub_type
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