Cell-type-specific modulation of Hox gene expression by members of the TGF-beta superfamily: a comparison between human osteosarcoma and neuroblastoma cell lines.

Abstract:

:Homeobox gene expression in osteoblast-like cells was investigated using the polymerase chain reaction (PCR). A total of 13 homeobox genes was detected in U-2 OS (human osteosarcoma) and MC3T3-E1 (mouse osteoblast) cells by sequencing cloned PCR products. Using specific primers, a different pattern of Hox gene expression was shown for the neuroblastoma cell line SK-N-SH relative to U-2 OS and MC3T3-E1. Additionally, we showed that expression of HOXC6 in U-2 OS and SK-N-SH was differentially regulated by rhBMP-2, TGF-beta and activin-A. This suggests that specific Hox genes may be target genes for TGF-beta superfamily members, and allows us to further understand the complex functions of these growth factors and how they relate to growth and development.

authors

Kloen P,Visker MH,Olijve W,van Zoelen EJ,Boersma CJ

doi

10.1006/bbrc.1997.6458

subject

Has Abstract

pub_date

1997-04-17 00:00:00

pages

365-9

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(97)96458-5

journal_volume

233

pub_type

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