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The appearance of phospholipase activity in the human macrophage-like cell line U937 during dimethyl sulfoxide induced differentiation.

Abstract：

:The human histiocyte cell line, U937, with monocyte characteristics, can be induced to differentiate into macrophage-like cells when exposed to growth medium containing 1.5% DMSO. Following three days of exposure, DMSO-treated but not control U937 cells can be stimulated to release endogenous arachidonic acid from their phospholipids. Maximum release of the unsaturated fatty acid occurs with 10 microM calcium ionophore in the presence but not in the absence of exogenously added calcium ion. In addition, DMSO-treated but not control U937 cells exhibit phospholipase activity when exposed to human IgG and then anti-human immunoglobulin. These data suggest that with respect to arachidonic acid metabolism U937 cells differentiate into functional macrophage-like cells when exposed to DMSO.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Myers RF,Siegel MI

doi

10.1016/0006-291x(84)91089-1

subject

Has Abstract

pub_date

1984-01-13 00:00:00

pages

217-24

issue

1

eissn

0006-291X

issn

1090-2104

pii

0006-291X(84)91089-1

journal_volume

118

pub_type

杂志文章

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