Abstract:
:We have previously demonstrated that antibodies to the growth-associated protein, GAP-43, introduced intracellularly using a lipid carrier inhibited neurite outgrowth in NB2a/d1 neuroblastoma cells, and that culturing of these cells on adhesive substrates such as laminin or poly-L-lysine overcame this restriction. These findings suggest that GAP-43 may facilitate neuritogenesis by increasing membrane adhesiveness. To address this issue, in the present study we examined the effect of intracellular delivery of this antibody on growth cone size. A statistically significant percentage of those neurites that did elaborate following intracellular delivery of GAP-43 exhibited either no observable growth cones or smaller growth cones versus cells receiving pre-immune IgG. These results support the hypothesis that the requirement for GAP-43 in neuritogenesis may be related to growth cone formation and membrane adhesiveness.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Shea TBdoi
10.1006/bbrc.1994.2204subject
Has Abstractpub_date
1994-08-30 00:00:00pages
459-64issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(84)72204-2journal_volume
203pub_type
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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更新日期:2000-12-20 00:00:00
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