Identification and recombinant production of human laminin alpha4 subunit splice variants.

abstract：:We showed previously that the neuropeptide, galanin, transiently and promptly increases the cytoplasmic concentration of Ca2+, [Ca2+]i, in insulin producing clonal RINm5F cells prior to a reduction in [Ca2+]i. We found that galanin (100 nM) transiently increased the [Ca2+]i in the presence of 15 mM glyceraldehyde and ...

journal_title：Biochemical and biophysical research communications

authors： Ahrén B

更新日期：1996-04-05 00:00:00

abstract：:Formation of SDS-insoluble protein aggregates in affected neurons is a cellular pathological feature of polyglutamine (polyQ) disease. We identified a multi-WD-domain protein, receptor for activated protein kinase C1 (RACK1), as a novel polyQ aggregate component from a Drosophila transgenic polyQ disease model. We sho...

journal_title：Biochemical and biophysical research communications

authors： Lam W,Chan WM,Lo TW,Wong AKY,Wu CC,Chan HYE

更新日期：2008-12-12 00:00:00

abstract：:WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The cloning and characterization of a novel human WW domain-containing gene, hWW45, which encodes a protein of app...

journal_title：Biochemical and biophysical research communications

authors： Valverde P

更新日期：2000-10-05 00:00:00

abstract：:Atrial natriuretic factor (ANF) rapidly enhanced phosphorylation of plasma membrane proteins of bovine adrenal cortical cells. Pretreatment of the membranes with ANF (1 x 10(-8)M to 1 x 10(-7)M) resulted two- to four-fold in an incorporation of 32p-radioactivity from [gamma -32p]ATP as compared to the controls. The gu...

journal_title：Biochemical and biophysical research communications

authors： Pandey KN

更新日期：1989-09-15 00:00:00

abstract：:Cytochrome b5 has been shown to stimulate, inhibit or have no effect on catalysis by P450 cytochromes. Its action is known to depend on the isozyme of cytochrome P450, the substrate, and experimental conditions. Cytochrome P450 2B4 (CYP 2B4) has been used in our laboratory as a model isozyme to study the role of cytoc...

journal_title：Biochemical and biophysical research communications

authors： Zhang H,Myshkin E,Waskell L

更新日期：2005-12-09 00:00:00

abstract：:C(2)-ceramide, a cell-permeable analog of ceramide, caused cell death in cultured rat cortical neuronal cells. C(2)-ceramide-induced neuronal loss was accompanied by upregulation of caspase-3 activity, measured by cleavage of its fluorogenic substrate Ac-DEVD-AMC. Similar results were obtained when cortical neuronal c...

journal_title：Biochemical and biophysical research communications

authors： Movsesyan VA,Yakovlev AG,Dabaghyan EA,Stoica BA,Faden AI

更新日期：2002-11-29 00:00:00

abstract：:Information about histone sequences and histone gene organization from invertebrate chordates is completely lacking. A genomic clone containing linked H3 and H4 histone genes from the urochordate Styela plicata was analyzed. The nucleotide sequence indicates that the two genes are transcribed in opposite directions an...

journal_title：Biochemical and biophysical research communications

authors： Ishaq AI,Rizvi SB,Wells DE,Tomlinson CR

更新日期：1993-07-30 00:00:00

abstract：:The synthesis of toxin protein from Ustilago maydis virus (UmV) strain P4 was studied in vitro and in vivo. The protein synthesized in vitro and in vivo has a molecular weight of approximately 30 kd whereas the native toxin has a molecular weight of about 12 kd. In the presence of protease inhibitors and glycosylation...

journal_title：Biochemical and biophysical research communications

authors： Podila GK,Bozarth RF,Flurkey WH

更新日期：1987-12-16 00:00:00

abstract：:Din7 is a DNA damage-inducible mitochondrial nuclease that modulates the stability of mitochondrial DNA (mtDNA) in Saccharomyces cerevisiae. How DIN7 gene expression is regulated, however, has remained largely unclear. Using promoter sequence alignment, we found a highly conserved 19-bp sequence in the promoter region...

journal_title：Biochemical and biophysical research communications

authors： Yoshitani A,Yoshida M,Ling F

更新日期：2008-01-04 00:00:00

abstract：:A hammerhead ribozyme directed against murine TNFalpha (mTNFalpha) mRNA has been constructed. In vitro studies showed that this ribozyme was released from the parent molecule by flanking cis-acting hammerhead and hairpin ribozymes. This same anti-mTNFalpha ribozyme specifically cleaved both synthetically derived subst...

journal_title：Biochemical and biophysical research communications

authors： MacKay SL,Tannahill CL,Auffenberg T,Ksontini R,Copeland EM 3rd,Moldawer LL

更新日期：1999-07-05 00:00:00

abstract：:5-Lipoxygenase (5LO) catalyzes two steps in the biosynthesis of leukotrienes (LTs), lipid mediators of inflammation derived from arachidonic acid. LTs function in normal host defense, and have pathophysiological roles in chronic inflammatory diseases as asthma and atherosclerosis. Also, possible effects of 5LO product...

journal_title：Biochemical and biophysical research communications

authors： Rådmark O,Samuelsson B

更新日期：2010-05-21 00:00:00

abstract：:An ubiquinone-binding protein (QP) was purified from mitochondrial NADH-ubiquinone reductase (Complex I). Complex I was separated into 3 fragments: a fraction of hydrophobic proteins, that of soluble iron-sulfur protein (IP) and soluble NADH dehydrogenase of flavoprotein by a procedure involving the resolution with DO...

journal_title：Biochemical and biophysical research communications

authors： Suzuki H,Ozawa T

更新日期：1986-08-14 00:00:00

abstract：:In a chicken B cell line, DT40, the disruption of HDAC2 (chHDAC2) gene causes an alteration of several gene expressions including chicken IgM light chain (chIgM-L) gene by 2D-PAGE analysis. To investigate the transcriptional function of chHDAC2, we employed the chIgM-L promoter reporter plasmid. We found that chHDAC2 ...

journal_title：Biochemical and biophysical research communications

authors： Takechi S,Adachi M,Nakayama T

更新日期：2002-11-29 00:00:00

abstract：:The effects of phenylalanine and tetrahydrobiopterin on the limited proteolysis of rat liver phenylalanine hydroxylase by chymotrypsin have been examined. The presence of tetrahydrobiopterin inhibits the proteolytic activation of native phenylalanine hydroxylase. In contrast, phenylalanine causes a stimulation of prot...

journal_title：Biochemical and biophysical research communications

authors： Phillips RS,Iwaki M,Kaufman S

更新日期：1983-02-10 00:00:00

abstract：:Myeloid leukemia factors (MLF1 and MLF2) are proteins associated with leukemia and several other cancers. However, little is known about the regulatory mechanisms underlying the stability of these proteins. Here, we show that DDB1 and CUL4 associated factor 8 (DCAF8), which can form a functional E3 ligase complex (CRL...

journal_title：Biochemical and biophysical research communications

authors： Huang D,Liu C,Sun X,Sun X,Qu Y,Tang Y,Li G,Tong T

更新日期：2020-08-20 00:00:00

abstract：:Recent in vitro and in vivo studies have shown that either animal- or human-derived embryonic stem cells can differentiate into insulin-secreting cells and lower blood glucose levels. However, studies utilizing human umbilical cord blood (HUCB) mononuclear cells to improve blood glucose levels in diabetic animals have...

journal_title：Biochemical and biophysical research communications

authors： Ende N,Chen R,Reddi AS

更新日期：2004-08-13 00:00:00

abstract：:The role of nutritional factors during CryIVA protoxin expression in Bacillus thuringiensis israelensis (Bti) has been investigated. Inorganic phosphate (Pi) was found to stimulate 135 kD protoxin synthesis by Bti cells. There was a corresponding increase in the cryIVA specific mRNA in the presence of Pi. Inorganic ph...

journal_title：Biochemical and biophysical research communications

authors： Banerjee-Bhatnagar N

更新日期：1999-08-27 00:00:00

abstract：:Spectroscopic analysis of the interaction of the epimeric 20-hydroperoxy derivatives of cholesterol with bovine adrenocortical cytochrome P-450scc preparations suggested the formation of a transient species. The intermediate was detected at 4 degrees C and characterized by a minimum at 412 nm in the difference spectru...

journal_title：Biochemical and biophysical research communications

authors： Larroque C,van Lier JE

更新日期：1983-04-29 00:00:00

abstract：:The calmodulin and C-kinase antagonists melittin, calmidazolium, N-(6-aminohexyl)-5-chloro-1-napthalenesulfonamide (W7), and trifluoperazine (TFP) also inhibit the activity of the human erythrocyte Ca2+-dependent protease, calpain I. W-5, the nonchlorinated derivative of W-7, was ineffective as an inhibitor of calpain...

journal_title：Biochemical and biophysical research communications

authors： Brumley LM,Wallace RW

更新日期：1989-03-31 00:00:00

abstract：:The Ca(2+)-binding S100A1 protein displays a specific and high expression level in the human myocardium and is considered to be an important regulator of heart contractility. Diminished protein levels detected in dilated cardiomyopathy possibly contribute to impaired Ca(2+) handling and contractility in heart failure....

journal_title：Biochemical and biophysical research communications

authors： Kiewitz R,Acklin C,Schäfer BW,Maco B,Uhrík B,Wuytack F,Erne P,Heizmann CW

更新日期：2003-06-27 00:00:00

abstract：:We have isolated, in guinea-pig endometrial cells, an estrogen-induced 1.8 kb RNA called gec1. Screening of a guinea-pig genomic library led to identification of gec1 gene consisting of 4 exons and 3 introns. Exon 1 contains the 5'UTR and the ATG initiation codon. A guinea-pig gec1 cDNA was obtained by 5'-RACE. The 35...

journal_title：Biochemical and biophysical research communications

authors： Vernier-Magnin S,Muller S,Sallot M,Radom J,Musard JF,Adami P,Dulieu P,Rémy-Martin JP,Jouvenot M,Fraichard A

更新日期：2001-06-01 00:00:00

abstract：:Herpes simplex virus-1 (HSV-1) glycoprotein E (gE) is a multifunctional protein capable of both binding the Fc portion of IgG and mediating cell-to-cell spread of HSV-1. Here we report that the domain on gE involved in IgG binding is distinct from the domain involved in mediating cell-to-cell spread. To do this we hav...

journal_title：Biochemical and biophysical research communications

authors： Weeks BS,Sundaresan P,Nagashunmugam T,Kang E,Friedman HM

更新日期：1997-06-09 00:00:00

abstract：:Co3O4 nanoparticles (NPs) are one kind of the important nanomaterials that have the application potential in catalyst, electrochromic devices, sensors, etc. However, their biological effect remains to be detailed. In this study, we investigated the effect of the as-synthesized Co3O4 NPs (15-30 nm) on the growth of mam...

journal_title：Biochemical and biophysical research communications

authors： Wang H,Ren T,Zhu N,Yu Q,Li M

更新日期：2018-11-02 00:00:00

abstract：BACKGROUND:Long non-coding RNAs (lncRNAs) have been demonstrated to be intensively involved in the development of various carcinomas, including glioblastoma multiforme (GBM). However, only a few of them have been well characterized. LncRNA CASP5 have been found to be up-regulated in GBM tissues compared with normal tis...

journal_title：Biochemical and biophysical research communications

authors： Zhou Y,Dai W,Wang H,Pan H,Wang Q

更新日期：2018-06-12 00:00:00

abstract：:MS-209 is a novel quinoline derivative reversing P-glycoprotein-mediated multidrug resistance (MDR). We investigated the interaction between MS-209 and multidrug resistance protein (MRP) in MRP-overexpressing human gastric cancer cells. We measured [3H]leukotriene C4 uptake into the membrane vesicles of the cells and ...

journal_title：Biochemical and biophysical research communications

authors： Nakamura T,Oka M,Aizawa K,Soda H,Fukuda M,Terashi K,Ikeda K,Mizuta Y,Noguchi Y,Kimura Y,Tsuruo T,Kohno S

更新日期：1999-02-24 00:00:00

abstract：:We report the identification of the full-length cDNA for a novel ATP-binding cassette (ABC) transporter from human macrophages. The mRNA is of 6.8 kb size and contains an open reading frame encoding a polypeptide of 2146 amino acids with a calculated molecular weight of 220 kDa. The predicted protein product is compos...

journal_title：Biochemical and biophysical research communications

authors： Kaminski WE,Orsó E,Diederich W,Klucken J,Drobnik W,Schmitz G

更新日期：2000-07-05 00:00:00

abstract：:Didemnin B, a cyclic N-methylated peptolide induces apoptosis in human HL-60 cells. When incubated with 1 microM didemnin B, unsynchronized HL-60 cultures undergo apoptosis to 100% within 140 minutes. Apoptosis has been assessed by the typical apoptotic morphology, the presence of double-stranded DNA fragments within ...

journal_title：Biochemical and biophysical research communications

authors： Grubb DR,Wolvetang EJ,Lawen A

更新日期：1995-10-24 00:00:00

abstract：:PLRG1 is a evolutionarily conserved protein in spliceosome and plays an important role in maintaining the integral part of the splicoeosme and its proper splicing. Here we solved the high resolution crystal structure of the WD40 domain of human PLRG1 by crystallography and compared our crystal structure with the cryo-...

journal_title：Biochemical and biophysical research communications

authors： Wang X,Li Y,Dai H,Xu C

更新日期：2021-01-01 00:00:00

abstract：:Mutations in the retinitis pigmentosa GTPase regulator (RPGR) gene, are the major cause of X-linked retinitis pigmentosa (RP), in which exon open reading frame 15 (ORF15) of RPGR has been implicated to play a substantial role. We identified a novel hemizygous missense mutation E585K of RPGR from whole-exome sequencing...

journal_title：Biochemical and biophysical research communications

authors： Liu YS,Pan JQ,Wan JF,Ren CY,Xu ZH,Pan XB,Gao RN,Liu SQ,Zhang JL,Yao QH,Wang JH,Li EM,Rao JH,Hou P,Chen JH

更新日期：2020-10-15 00:00:00

abstract：:The gene ENOD40 is expressed at an early stage of root nodule organogenesis and has been postulated to play a central regulatory role in the Rhizobium-legume interaction. In vitro translation of soybean ENOD40 mRNA showed that the gene encodes two peptides of 12 and 24aa residues (peptides A and B) that bind to sucros...

journal_title：Biochemical and biophysical research communications

authors： Röhrig H,John M,Schmidt J

更新日期：2004-12-17 00:00:00