Ligand effects on the limited proteolysis of phenylalanine hydroxylase: evidence for multiple conformational states.

abstract：:Increasing evidence shows that immune-mediated mechanisms may contribute to the pathogenesis of central nervous system disorders including cerebellar ataxias, as indicated by the aberrant production of neuronal surface antibodies. We previously reported a patient with cerebellar ataxia associated with production of a ...

journal_title：Biochemical and biophysical research communications

authors： Yaguchi H,Yabe I,Takahashi H,Watanabe M,Nomura T,Kano T,Matsumoto M,Nakayama KI,Watanabe M,Hatakeyama S

更新日期：2017-12-09 00:00:00

abstract：:We previously showed that NDP52 (also known as calcoco2) plays a role as an autophagic receptor for phosphorylated tau facilitating its clearance via autophagy. Here, we examined the expression and association of NDP52 with autophagy-regulated gene (ATG) proteins including LC3, as well as phosphorylated tau and amyloi...

journal_title：Biochemical and biophysical research communications

authors： Kim S,Lee D,Song JC,Cho SJ,Yun SM,Koh YH,Song J,Johnson GV,Jo C

更新日期：2014-11-07 00:00:00

abstract：:We proposed to use a novel stepwise sequence-constructive SELEX method to develop DNA aptamers that can recognize Globo H which is a tumor-associated carbohydrate antigen. A combinatorial synthetic library that consisted of DNA molecules with randomized regions of 15-bases was used as the starting library for the firs...

journal_title：Biochemical and biophysical research communications

authors： Wang CY,Wu CY,Hung TC,Wong CH,Chen CH

更新日期：2014-09-26 00:00:00

abstract：:In the presence of UDPglucose, rabbit muscle phosphofructokinase appeared to use PPi as a phosphoryl donor, as reported previously (Biochem. Biophys. Res. Commun. 121, 842-847). This apparent activity was due to conversion of UDPglucose and PPi to glucose 1-phosphate and UTP, the latter being metabolized by phosphofru...

journal_title：Biochemical and biophysical research communications

authors： Kruger NJ,Dennis DT

更新日期：1985-01-16 00:00:00

abstract：:We have screened genomic DNA for the identification of D4 dopamine receptor polymorphisms. We show that the D4 dopamine receptor genotype in 72 severely affected chronic alcoholics is heterogeneous, with individuals homozygous and heterozygous for the various D4 receptor alleles. Alcoholics demonstrated a greater prev...

journal_title：Biochemical and biophysical research communications

authors： George SR,Cheng R,Nguyen T,Israel Y,O'Dowd BF

更新日期：1993-10-15 00:00:00

abstract：:Estrogen depletion causes postmenopausal osteoporosis. Here we report that steady state mRNA levels of transforming growth factor-beta 1 (TGF-beta 1) and osteocalcin in bone persistently decreased and increased, respectively, in vivo in estrogen-depleted rats after ovariectomy (OVX). 21 female Wistar rats (7-month-old...

journal_title：Biochemical and biophysical research communications

authors： Ikeda T,Shigeno C,Kasai R,Kohno H,Ohta S,Okumura H,Konishi J,Yamamuro T

更新日期：1993-08-16 00:00:00

abstract：:Islet transplantation corrects chronic hyperglycemia by augmentation of insulin supply from the graft tissue, but the role of endogenous beta-cells after transplantation is not clear. In the present study, we examined endogenous beta-cell function after glucose homeostasis had been reestablished by islet graft in stre...

journal_title：Biochemical and biophysical research communications

authors： Hamamoto Y,Tsuura Y,Fujimoto S,Nagata M,Takeda T,Mukai E,Fujita J,Yamada Y,Seino Y

更新日期：2001-09-14 00:00:00

abstract：:CD44 is a major cell-surface receptor for hyaluronate (HA). By alternative RNA-splicing a large number of CD44 variants are generated. To explore the role of CD44 splicing in the regulation of cell binding to HA, three different isoforms of CD44 were transfected in the CD44 negative B-cell lymphoma line Namalwa and in...

journal_title：Biochemical and biophysical research communications

authors： van der Voort R,Manten-Horst E,Smit L,Ostermann E,van den Berg F,Pals ST

更新日期：1995-09-05 00:00:00

abstract：:Phosphorylation of a 25 Kdalton nuclear stress protein from rat muscle was examined by two-dimensional gel electrophoresis and one-dimensional peptide mapping. These studies show that three 25 Kdalton stress proteins found in stressed rat myoblasts are actually the same protein with charge variation brought about by m...

journal_title：Biochemical and biophysical research communications

authors： Kim YJ,Shuman J,Sette M,Przybyla A

更新日期：1983-12-28 00:00:00

abstract：:Voltage-gated potassium (K+) channel sub-family B member 1 (KCNB1, Kv2.1) is known to undergo oxidation-induced oligomerization during aging but whether this process affects brain's physiology was not known. Here, we used 10, 16 and 22 month-old transgenic mice overexpressing a KCNB1 variant that does not oligomerize ...

journal_title：Biochemical and biophysical research communications

authors： Yu W,Zhang H,Shin MR,Sesti F

更新日期：2019-05-14 00:00:00

abstract：:Vasopressin (VP) rapidly increased 45Ca2+ efflux. A VP antagonist prevented VP from mobilizing Ca2+ and stimulating DNA synthesis. Prostaglandin F2 alpha (PGF2 alpha) also stimulated rapid 45Ca2+ release. The effectiveness of different prostaglandins corresponded to their effectiveness as mitogens. The removal of exte...

journal_title：Biochemical and biophysical research communications

authors： Smith JB,Smith L

更新日期：1984-09-17 00:00:00

abstract：:When the F1-ATPase from the thermophilic bacterium, PS3, was inactivated by 90% with 7-chloro-4-nitro[14C]benzofurazan ([14C]Nbf-Cl) at pH 7.3 and then gel-filtered, 1.25 mols of [14C]Nbf-O-Tyr and less than 0.1 mol of Nbf-N-Lys were formed per mol of enzyme. After adjusting the pH of the gel-filtered, modified enzyme...

journal_title：Biochemical and biophysical research communications

authors： Andrews WW,Yoshida M,Hill FC,Allison WS

更新日期：1984-09-28 00:00:00

abstract：:Cep169 is a centrosomal protein conserved among vertebrates. In our previous reports, we showed that mammalian Cep169 interacts and collaborates with CDK5RAP2 to regulate microtubule (MT) dynamics and stabilization. Although Cep169 is required for MT regulation, its precise cellular function remains largely elusive. H...

journal_title：Biochemical and biophysical research communications

authors： Mori Y,Inoue Y,Taniyama Y,Tanaka S,Terada Y

更新日期：2015-12-25 00:00:00

abstract：:Ammonia-forming cytochrome c nitrite reductase from Sulfurospirillum deleyianum contains four covalently bound heme c groups/55 kDa subunit as determined by atomic absorption spectroscopy and the pyridine Fe(II)-hemochrome technique. Nitrite reductase was isolated from the membrane fraction as a monomer (M(r) 55 +/- 2...

journal_title：Biochemical and biophysical research communications

authors： Schumacher W,Hole U,Kroneck PM

更新日期：1994-11-30 00:00:00

abstract：:The metallo-disintegrins (ADAMs) are a family of mammalian proteins with significant amino acid sequence identity and a domain organisation similar to the snake venom metalloproteinases (reprolysins). They have been implicated in a wide variety of processes such as cell-cell and cell matrix adhesion and proteolysis of...

journal_title：Biochemical and biophysical research communications

authors： Millichip MI,Dallas DJ,Wu E,Dale S,McKie N

更新日期：1998-04-17 00:00:00

abstract：:CYP1A1 is largely involved in carcinogenesis through the bioactivation of numerous procarcinogens. Exposure to environmental pollutants such as polycyclic aromatic hydrocarbons (PAHs) leads to induction of CYP1A1 via AhR pathway. We have previously demonstrated that fetal bovine serum (FBS) induces CYP1A1 gene transcr...

journal_title：Biochemical and biophysical research communications

authors： N'Guyen QB,Fallone F,Seree E,Fina F,Villard PH,Guigal N,De Meo M,Lacarelle B,Martin PM,Barra Y

更新日期：2002-09-20 00:00:00

abstract：:mRNA levels of mitogen-activated protein kinase phosphatases, MKP-1 and MKP-2, were determined during chemical hepatocarcinogenesis and during regeneration of rat liver. In chemical hepatocarcinogenesis, the mRNA levels of MKP-1 were increased in primary hepatomas but decreased in rat ascites hepatomas as compared wit...

journal_title：Biochemical and biophysical research communications

authors： Yokoyama A,Karasaki H,Urushibara N,Nomoto K,Imai Y,Nakamura K,Mizuno Y,Ogawa K,Kikuchi K

更新日期：1997-10-29 00:00:00

abstract：:Ornithine transcarbamylase activity and immunoreactive enzyme level are compared during perinatal period and in adult rat. Ornithine transcarbamylase activity regularly rises during late fetal period and presents a marked increase 24 hours after birth. Immunoreactive enzyme level does not correlate with this developme...

journal_title：Biochemical and biophysical research communications

authors： Belbekouche M,Gautier C,Vaillant R

更新日期：1985-06-28 00:00:00

abstract：:A screening system comprised of a randomized hybrid-ribozyme library has previously been used to identify pro-death genes in Fas-mediated apoptosis, and short sequence information of candidate genes from this system was previously reported by Kawasaki and Taira [H. Kawasaki, K. Taira, A functional gene discovery in th...

journal_title：Biochemical and biophysical research communications

authors： Lee MR,Shin JN,Moon AR,Park SY,Hong G,Lee MJ,Yun CW,Seol DW,Piya S,Bae J,Oh JW,Kim TH

更新日期：2008-06-06 00:00:00

abstract：:CYP27A1 catalyses hydroxylations in the biosynthesis of bile acids and the bioactivation of vitamin D3. We investigated the expression of CYP27A1 in human monocytes, monocyte-derived macrophages, and dendritic cells on mRNA and protein levels as well as its enzymatic activity in comparison with the expression of CYP27...

journal_title：Biochemical and biophysical research communications

authors： Gottfried E,Rehli M,Hahn J,Holler E,Andreesen R,Kreutz M

更新日期：2006-10-13 00:00:00

abstract：:Fusing recombinant proteins to highly soluble partners is frequently used to prevent aggregation of recombinant proteins in Escherichia coli. Moreover, co-overexpression of prokaryotic chaperones can increase the amount of properly folded recombinant proteins. To understand the solubility enhancement of fusion protein...

journal_title：Biochemical and biophysical research communications

authors： Douette P,Navet R,Gerkens P,Galleni M,Lévy D,Sluse FE

更新日期：2005-08-05 00:00:00

abstract：:Neurons in the adult central nervous system (CNS) have a poor intrinsic axon growth potential after injury, but the underlying mechanisms are largely unknown. Wingless-related mouse mammary tumor virus integration site (WNT) family members regulate neural stem cell proliferation, axon tract and forebrain development i...

journal_title：Biochemical and biophysical research communications

authors： Duan RS,Liu PP,Xi F,Wang WH,Tang GB,Wang RY,Saijilafu,Liu CM

更新日期：2018-05-05 00:00:00

abstract：:The MUC1 transmembrane glycoprotein is aberrantly overexpressed in diverse human carcinomas and has been shown to inhibit apoptosis induced by genotoxic agents. In the present work, we report that MUC1 binds to and activates JNK1, an important member of the mitogen-activated protein kinases (MAPK) superfamily. The phy...

journal_title：Biochemical and biophysical research communications

authors： Chen Q,Li D,Ren J,Li C,Xiao ZX

更新日期：2013-10-11 00:00:00

abstract：:Moderate alcohol consumption has been shown to reduce atherosclerosis-associated diseases. As shown in our earlier works, ethanol has a dose-dependent protective effects against endothelial cellular senescence by activating aldehyde dehydrogenase 2 (ALDH2) in vitro. However, whether ethanol administration possesses an...

journal_title：Biochemical and biophysical research communications

authors： Xue L,Zhu W,Yang F,Dai S,Han Z,Xu F,Guo P,Chen Y

更新日期：2019-04-30 00:00:00

abstract：:We combined normal mode analysis (NMA) with cavity calculations as a method to get more insight into static crystal structures. We used nitrile hydratase (NHase) as a case study, and the crystal structure of a complex of Pseudonocardia thermophila NHase (1UGP) with n-butyric acid was chosen as a reference structure. T...

journal_title：Biochemical and biophysical research communications

authors： Taştan Bishop AO,Sewell T

更新日期：2006-04-28 00:00:00

abstract：:Pluripotent embryonic stem (ES) cells are the most versatile cells, with the potential to differentiate into all types of cell lineages including neural precursor cells (NPCs), which can be expanded in large numbers for significant periods of time to provide a reliable cell source for transplantation in neurodegenerat...

journal_title：Biochemical and biophysical research communications

authors： Xu H,Fan X,Wu X,Tang J,Yang H

更新日期：2005-01-07 00:00:00

abstract：:To identify endoproteolytic activity that processes the enkephalin precursor, a novel approach was undertaken for the production of model substrate in the form of recombinant 35S-(Met)-preproenkephalin (35S-(Met)-PPE), generated by in vitro transcription and translation of the rat PPE cDNA. Endoproteolytic activity in...

journal_title：Biochemical and biophysical research communications

authors： Hook VY,Hegerle D,Affolter HU

更新日期：1990-03-16 00:00:00

abstract：:The molecular mechanisms of multiple myeloma are not well defined. EEN is an endocytosis-regulating molecule. Here we report that EEN regulates the proliferation and survival of multiple myeloma cells, by regulating IGF-1 secretion. In the present study, we observed that EEN expression paralleled with cell proliferati...

journal_title：Biochemical and biophysical research communications

authors： Huang EW,Xue SJ,Li XY,Xu SW,Cheng JD,Zheng JX,Shi H,Lv GL,Li ZG,Li Y,Liu CH,Chen XH,Liu H,Li J,Liu C

更新日期：2014-05-02 00:00:00

abstract：:TREK1 is a member of the tandem-P domain K+ channel family which is expressed almost exclusively in the nervous system. It is modulated by a number of important factors including arachidonic acid and cell swelling. Since both factors are associated with brain ischemia, it has been suggested that activation of TREK1 ma...

journal_title：Biochemical and biophysical research communications

authors： Miller P,Kemp PJ,Peers C

更新日期：2005-06-17 00:00:00

abstract：:The relative inability of hemopoietic stem cells (HSCs) to reproduce themselves (self-renew) ex vivo imposes substantial limitations on the current use of HSC transplantation. Recently, the transcription factor Zfx has been demonstrated that played an important in controlling the self-renewal of hematopoietic stem cel...

journal_title：Biochemical and biophysical research communications

authors： Xu C,Zhang Y,Jiang H

更新日期：2009-02-13 00:00:00