IL-4-induced AID expression and its relevance to IgA class switch recombination.


:Activation-induced cytidine deaminase (AID) is an inducible gene that plays a critical role in Ig class switch recombination and somatic hypermutation in B cells. We explored the mechanisms by which IL-4 induces AID expression in mouse B cells. IL-4 increased AID expression and over-expression of Stat6 further augmented IL-4-induced promoter activity. The involvement of Stat6 in the promoter activity was confirmed using ChIP assays and site-directed mutagenesis. Treatment with H89, a PKA inhibitor, markedly decreased IL-4-induced AID expression, and over-expression of CREB enhanced it. These results indicate that Stat6 and PKA/CREB are involved in IL-4-induced AID expression. The relevance of these signal transducing molecules was verified using the TGFbeta1-induced IgA isotype switching model. Our results indicate that IL-4, through Stat6 and PKA/CREB, induces AID expression leading to Ig isotype switching event.


Kim RJ,Kim HA,Park JB,Park SR,Jeon SH,Seo GY,Seo DW,Seo SR,Chun GT,Kim NS,Yie SW,Byeon WH,Kim PH




Has Abstract


2007-09-21 00:00:00














  • Cyclin A regulates a cell-cycle-dependent expression of CKAP2 through phosphorylation of Sp1.

    abstract::CKAP2 plays crucial roles in proper chromosome segregation and maintaining genomic stability. CKAP2 protein showed cell-cycle-dependent expression, which reached a maximum level at the G2/M phase and disappeared at the onset of G1 phase. To elucidate the mechanisms underlying cell cycle-dependent expression of CKAP2, ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kang DS,Hong KM,Park J,Bae CD

    更新日期:2012-04-20 00:00:00

  • Involvement of Rabring7 in EGF receptor degradation as an E3 ligase.

    abstract::Rab7, a member of the Rab family small G proteins, is involved in the late stage of the endocytic pathway. We previously identified a Rab7 target protein, Rabring7, which contains a RING finger domain at its C termini, but the precise role of Rabring7 remains unknown. In this study, we demonstrate using an in vitro ub...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sakane A,Hatakeyama S,Sasaki T

    更新日期:2007-06-15 00:00:00

  • Role of intracellular Ca2+ in the epidermal growth factor induced inhibition of protein tyrosine phosphatase activity in a breast cancer cell line.

    abstract::Increased protein tyrosyl phosphorylation in response to growth factors has been assumed to be solely due to activation of protein tyrosine kinases (PTKs). We report that total cellular protein tyrosine phosphatase (PTPase) activity declined in MDA-MB 468 breast carcinoma cells exposed to epidermal growth factor (EGF)...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mishra S,Hamburger AW

    更新日期:1993-03-31 00:00:00

  • Role of S6K1 in regulation of SREBP1c expression in the liver.

    abstract::The transcription factor sterol regulatory element-binding protein 1c (SREBP1c) plays an important role in the control of fatty acid metabolism in the liver. Evidence suggests that mammalian target of rapamycin (mTOR) complex 1 (mTORC1) contributes to the regulation of SREBP1c expression, but signaling downstream of m...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Li S,Ogawa W,Emi A,Hayashi K,Senga Y,Nomura K,Hara K,Yu D,Kasuga M

    更新日期:2011-08-26 00:00:00

  • Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ.

    abstract::We here isolated and characterized a cDNA clone encoding 40-kDa heat-shock protein hsp40 from cDNA expression library of human placenta by immunoscreening with anti-hsp40 antibody. N-terminal amino acid sequence (48 residues) deduced from the base sequence was completely identical to that of hsp40 purified from HeLa c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ohtsuka K

    更新日期:1993-11-30 00:00:00

  • Ang II induces capillary formation from endothelial cells via the AT1R-dependent inositol requiring enzyme 1 pathway.

    abstract::Previous studies have demonstrated an important interaction between angiotension II type 1 receptor (AT1R) and angiotension II (Ang II) -induced capillary formation from endothelial cells and vascular endothelial growth factor (VEGF). However, the underlying mechanism remains elusive. Recent studies revealed that the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang X,Bai YP,Hong D,Gao HC,Li LF,Li CC,Zhu LP,Sun Q,Zhang GG

    更新日期:2013-05-10 00:00:00

  • Angiogenic effects of dual gene transfer of bFGF and PDGF-BB after myocardial infarction.

    abstract::Therapeutic effects of combination of angiogenic growth factors for the treatment of ischemia after myocardial infarction are largely unknown. Plasmids expressing basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF-BB) or their combination with a 1:1 mass ratio were injected into hearts with 7-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hao X,Månsson-Broberg A,Gustafsson T,Grinnemo KH,Blomberg P,Siddiqui AJ,Wärdell E,Sylvén C

    更新日期:2004-03-19 00:00:00

  • Normal activation of p70 S6 kinase by insulin in cells overexpressing dominant negative 85kD subunit of phosphoinositide 3-kinase.

    abstract::The role of heteromeric phosphoinositide (PI) 3-kinase activity in insulin signal transduction was studied by investigating the effects of (i) overexpression of a dominant negative mutant p85 (delta p85) that lacks the binding site for p110 (delta p85-overexpressing cells) and (ii) inhibition of PI 3-kinase activity b...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hara K,Yonezawa K,Sakaue H,Kotani K,Kotani K,Kojima A,Waterfield MD,Kasuga M

    更新日期:1995-03-17 00:00:00

  • Antibody to human lymphocyte actin regulates immunoglobulin secretion by an EBV-transformed human B-cell line.

    abstract::Antibody against actin isolated from a human EBV-transformed lymphoblastoid B-cell line exerted an inhibitory effect on in vitro IgM secretion by a different lymphoblastoid B-cell line, LA350. This effect was dose dependent showing from 24-40% inhibition at a dilution of 1:100 and 68-80% inhibition at a dilution of 1:...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rosenblatt HM,Green CG,McClure JE,Shearer WT

    更新日期:1986-10-15 00:00:00

  • Silencing MR-1 attenuates inflammatory damage in mice heart induced by AngII.

    abstract::Myofibrillogenesis regulator-1(MR-1) can aggravate cardiac hypertrophy induced by angiotensin(Ang) II in mice through activation of NF-kappaB signaling pathway, and nuclear transcription factor (NF)-kappaB and activator protein-1(AP-1) regulate inflammatory and immune responses by increasing the expression of specific...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dai W,Chen H,Jiang J,Kong W,Wang Y

    更新日期:2010-01-15 00:00:00

  • Identification of AHCY inhibitors using novel high-throughput mass spectrometry.

    abstract::S-adenosylhomocysteine hydrolase (AHCY) catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to adenosine and l-homocysteine. This enzyme is frequently overexpressed in many tumor types and is considered to be a validated anti-tumor target. In order to enable the development of small molecule AHCY inhib...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Uchiyama N,Dougan DR,Lawson JD,Kimura H,Matsumoto SI,Tanaka Y,Kawamoto T

    更新日期:2017-09-09 00:00:00

  • Glycogenolysis is directed towards ascorbate synthesis by glutathione conjugation.

    abstract::Using isolated rat hepatocytes we have shown that glutathione (GSH) depletion by glutathione-S-transferase (GST)-catalyzed conjugation with 1-bromoheptane or phorone was accompanied by a significant elevation in ascorbate synthesis. Glycogenolysis was also stimulated without a significant rise in glucose synthesis. Fu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chan TS,Wilson JX,O'Brien PJ

    更新日期:2004-04-23 00:00:00

  • HMGB1-containing nucleosome mediates chemotherapy-induced metastasis of human lung cancer.

    abstract::While chemotherapy is an important and widely used therapeutic for cancer, it may facilitate cancer metastasis. Herein, we report that human lung cancer cells exert higher invasion and metastasis after chemotherapy. In a human lung cancer xenograft model, chemotherapy promotes the cancer invasion and metastasis in HMG...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang K,Shan S,Wang S,Gu X,Zhou X,Ren T

    更新日期:2018-06-07 00:00:00

  • Cyclic strain-induced endothelial MMP-2: role in vascular smooth muscle cell migration.

    abstract::Matrix metalloproteinases (MMPs) play a vital role in vasculature response to hemodynamic stimuli via the degradation of extracellular matrix substrates. In this study, we investigated the putative role of cyclic strain-induced endothelial MMP-2 (and MMP-9) expression and release in modulating bovine aortic smooth mus...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: von Offenberg Sweeney N,Cummins PM,Birney YA,Redmond EM,Cahill PA

    更新日期:2004-07-23 00:00:00

  • Structure of an unconventional SH3 domain from the postsynaptic density protein Shank3 at ultrahigh resolution.

    abstract::The Shank family comprises three large multi-domain proteins playing central roles as protein scaffolds in the neuronal postsynaptic density. The Shank proteins are closely linked to neuropsychiatric diseases, such as autism spectrum disorders. One characteristic domain in the Shank family is the SH3 domain, assumed t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ponna SK,Myllykoski M,Boeckers TM,Kursula P

    更新日期:2017-08-26 00:00:00

  • Studies on immunoproteasome in human liver. Part I: absence in fetuses, presence in normal subjects, and increased levels in chronic active hepatitis and cirrhosis.

    abstract::Despite the central role of proteasomes in relevant physiological pathways and pathological processes, this topic is unexpectedly largely unexplored in human liver. Here we present data on the presence of proteasome and immunoproteasome in human livers from normal adults, fetuses and patients affected by major hepatic...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Vasuri F,Capizzi E,Bellavista E,Mishto M,Santoro A,Fiorentino M,Capri M,Cescon M,Grazi GL,Grigioni WF,D'Errico-Grigioni A,Franceschi C

    更新日期:2010-06-25 00:00:00

  • Imaging mass spectroscopy delineates the thinned and thickened walls of intracranial aneurysms.

    abstract:OBJECT:The wall thickness of intracranial aneurysms (IAs) is heterogeneous. Although thinning of the IA wall is thought to contribute to IA rupture, the underlying mechanism remains poorly understood. Recently, imaging mass spectroscopy (IMS) has been used to reveal the distribution of phospholipids in vascular disease...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ikedo T,Minami M,Kataoka H,Hayashi K,Nagata M,Fujikawa R,Yamazaki F,Setou M,Yokode M,Miyamoto S

    更新日期:2018-01-01 00:00:00

  • Application of spontaneously immortalized odontoblast cells in tooth regeneration.

    abstract::Here, we report on the first attempt to bioengineer tooth using a spontaneously immortalized mesenchymal cell line. To assess the odontogenic potential of this cell line, odontoblast-lineage cells (OLC) were re-associated with competent dental epithelium isolated from E14.5 mice. A novel three-dimensional organ germ c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Arany S,Kawagoe M,Sugiyama T

    更新日期:2009-03-27 00:00:00

  • Involvement of WNK1-mediated potassium channels in the sexual dimorphism of blood pressure.

    abstract::Potassium homeostasis plays an essential role in the control of blood pressure. It is unknown, however, whether potassium balance is involved in the gender-associated blood pressure differences. We therefore investigated the possible mechanism of sexual dimorphism in blood pressure regulation by measuring the blood pr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yu G,Cheng M,Wang W,Zhao R,Liu Z

    更新日期:2017-04-01 00:00:00

  • Activation of bovine photoreceptor guanylate cyclase by S100 proteins.

    abstract::S100 proteins are acidic calcium-binding proteins present in high concentration in the brain and at lower concentrations in peripheral tissues. Their function remains to be elucidated in many tissues. In this report we show for the first time that S100 proteins stimulate the bovine photoreceptor membrane guanylate cyc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Margulis A,Pozdnyakov N,Sitaramayya A

    更新日期:1996-01-05 00:00:00

  • Biophysical characterization of the breast cancer-related BIG3-PHB2 interaction: Effect of non-conserved loop region of BIG3 on the structure and the interaction.

    abstract::Brefeldin A-inhibited guanine nucleotide-exchange protein 3 (BIG3) interacts with and inhibits the tumor suppressor function of prohibitin-2 (PHB2), and recent in vivo studies have demonstrated that the BIG3-PHB2 interaction is a promising target for breast cancer therapy. However, little biophysical characterization ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chigira T,Nagatoishi S,Takeda H,Yoshimaru T,Katagiri T,Tsumoto K

    更新日期:2019-10-08 00:00:00

  • Expression and characterization of the tau subunit of phosphorylase kinase.

    abstract::A cDNA encoding the entire tau subunit of rabbit skeletal muscle phosphorylase kinase was reconstructed and inserted into a plasmid containing the Escherichia coli ptac promoter and a constructed plasmid containing the ptac promoter and bacterial chloramphenicol acetyl transferase (CAT) gene, respectively. A significa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chen LR,Yuan CJ,Somasekhar G,Wejksnora P,Peterson JE,Myers AM,Graves L,Cohen PT,da Cruz e Silva EF,Graves DJ

    更新日期:1989-06-15 00:00:00

  • Pyrophosphate functions as phosphoryl donor with UDP-glucose-treated mammalian phosphofructokinase.

    abstract::Phosphofructokinase of rabbit muscle, which is specific for nucleoside triphosphates such as ATP, dissociated and gained the capability to utilize pyrophosphate as phosphoryl donor following incubation with UDP-glucose. The pyrophosphate- and ATP-linked activities of UDP-glucose-treated muscle phosphofructokinase were...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wong JH,Balogh A,Buchanan BB

    更新日期:1984-06-29 00:00:00

  • Combined enhancement of microtubule assembly and glucose metabolism in neuronal systems in vitro: decreased sensitivity to copper toxicity.

    abstract::Brain cell-free extract greatly stimulates the polymerization rate of purified tubulin with a reduction of the nucleation period and without a significant alteration of the final assembly state. This effect is mimicked by neuroblastoma extract at 10-fold lower extract concentration, but not by excess muscle extract. C...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Liliom K,Wágner G,Kovács J,Comin B,Cascante M,Orosz F,Ovádi J

    更新日期:1999-10-22 00:00:00

  • Molecular cloning of human GRB-7 co-amplified with CAB1 and c-ERBB-2 in primary gastric cancer.

    abstract::Many amplified-chromosome regions in human cancers have been identified, while there are only a limited number of reports for amplified genes in a single amplicon. We recently isolated four cDNA clones, A39, B47, C51, and CAB1, from 500 kilobases of yeast artificial chromosome DNA containing the c-ERBB-2 gene. B47 con...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kishi T,Sasaki H,Akiyama N,Ishizuka T,Sakamoto H,Aizawa S,Sugimura T,Terada M

    更新日期:1997-03-06 00:00:00

  • Activation of prothrombin by two subtilisin-like serine proteases from Acremonium sp.

    abstract::Two novel subtilisin-like serine proteases (AS-E1 and -E2) that activate prothrombin have been identified in a culture of the fungus Acremonium sp. The enzymes were purified through repeated hydrophobic interaction chromatography. The N-terminal sequences of AS-E1 (34.4 kDa) and AS-E2 (32 kDa) showed high similarity t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Liu C,Matsushita Y,Shimizu K,Makimura K,Hasumi K

    更新日期:2007-06-22 00:00:00

  • Expression of Smads during in vitro transdifferentiation of hepatic stellate cells to myofibroblasts.

    abstract::TGFbeta is of crucial importance during transdifferentiation of resting retinoid-storing hepatic stellate cells (HSC) to extracellular matrix producing myofibroblasts (MFB) and consequently, inhibition of TGFbeta signal transduction is an effective means for preventing experimental fibrosis. We have shown that isolate...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dooley S,Streckert M,Delvoux B,Gressner AM

    更新日期:2001-05-11 00:00:00

  • Carbohydrate antigens recognized by anti-horseradish peroxidase antiserum are expressed on mammalian cells.

    abstract::Antiserum raised against horseradish peroxidase (HRP) recognizes neural specific carbohydrate antigens in Drosophila and other insects. The occurrence of the antigens in mammalian cells was investigated. Proteins were extracted from rat pheochromocytoma cells, PC12, and various tissue of rat and bovine origin, and fur...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kurosaka A,Tsuyuki Y,Tahara S

    更新日期:1994-10-14 00:00:00

  • Identification of a novel stress regulated FERM domain containing cytosolic protein having PTP activity in Setaria cervi, a bovine filarial parasite.

    abstract::A 67 kDa cytosolic FERM domain containing protein having significant protein tyrosine phosphatases activity (PTPL) has been purified to homogeneity from Setaria cervi, a bovine filarial parasite. The MALDI-MS/MS analysis of the purified protein revealed 16 peptide peaks showing nearest match to Brugia malayi Moesin/ez...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Singh N,Heneberg P,Singh N,Singh SK,Rathaur S

    更新日期:2015-02-27 00:00:00

  • Characterization of the binding of the RNA-binding protein AUF1 to the human AT(1) receptor mRNA.

    abstract::An important mechanism of regulation of the expression of the AT(1) receptors is the modulation of the mRNA stability. AUF1, a human RNA-binding protein, may play an important role. Since AUF1 seems to bind to AU-rich regions of the 3'-untranslated region of the mRNAs, we verified the nucleotide sequence of human AT(1...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pende A,Giacchè M,Castigliola L,Contini L,Passerone G,Patrone M,Port JD,Lotti G

    更新日期:1999-12-20 00:00:00