Abstract:
:Activation-induced cytidine deaminase (AID) is an inducible gene that plays a critical role in Ig class switch recombination and somatic hypermutation in B cells. We explored the mechanisms by which IL-4 induces AID expression in mouse B cells. IL-4 increased AID expression and over-expression of Stat6 further augmented IL-4-induced promoter activity. The involvement of Stat6 in the promoter activity was confirmed using ChIP assays and site-directed mutagenesis. Treatment with H89, a PKA inhibitor, markedly decreased IL-4-induced AID expression, and over-expression of CREB enhanced it. These results indicate that Stat6 and PKA/CREB are involved in IL-4-induced AID expression. The relevance of these signal transducing molecules was verified using the TGFbeta1-induced IgA isotype switching model. Our results indicate that IL-4, through Stat6 and PKA/CREB, induces AID expression leading to Ig isotype switching event.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Kim RJ,Kim HA,Park JB,Park SR,Jeon SH,Seo GY,Seo DW,Seo SR,Chun GT,Kim NS,Yie SW,Byeon WH,Kim PHdoi
10.1016/j.bbrc.2007.07.022subject
Has Abstractpub_date
2007-09-21 00:00:00pages
398-403issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(07)01472-6journal_volume
361pub_type
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