Reduction of nitric oxide producing activity associated with in vitro aging in cultured human umbilical vein endothelial cell.

abstract：:Our prior studies showed that certain cobalamins inhibit productive HIV-1 infection of primary cultures of blood lymphocytes and monocytes. We demonstrate here that this antiviral activity may be mediated by an inhibition of HIV-1 integrase, an enzyme required for productive infection. Purified recombinant HIV-1 integ...

journal_title：Biochemical and biophysical research communications

authors： Weinberg JB,Shugars DC,Sherman PA,Sauls DL,Fyfe JA

更新日期：1998-05-19 00:00:00

abstract：:We investigated the effects of oxidative stress on mRNA levels of heparin-binding epidermal growth factor-like growth factor (HB-EGF) and amphiregulin (AR) in rat gastric epithelial RGM1 cells. In response to stimulation with hydrogen peroxide (100-400 microM), gene expression of HB-EGF and AR increased in a dose-depe...

journal_title：Biochemical and biophysical research communications

authors： Miyazaki Y,Shinomura Y,Tsutsui S,Yasunaga Y,Zushi S,Higashiyama S,Taniguchi N,Matsuzawa Y

更新日期：1996-09-13 00:00:00

abstract：:ADP-ribosylation by cholera toxin of the guanine nucleotide binding regulatory protein (Gs) of rat liver membrane adenylate cyclase was inhibited by 0.1-1 mM MDL 12330A or 0.1-1 mM chlorpromazine. Basal as well as cholera toxin activated adenylate cyclase activity in liver membranes was also inhibited by the two drugs...

journal_title：Biochemical and biophysical research communications

authors： Bitonti AJ

更新日期：1984-04-30 00:00:00

abstract：:In the present study we used LLC-PK1 cells, a porcine renal proximal tubular cell line, to investigate whether PI3 kinase activation was involved in the anti-apoptotic effect of ouabain, a specific inhibitor of Na,K-ATPase. Apoptosis was induced by actinomycin D (Act D, 5 microM) and assessed by appearance of hypodipl...

journal_title：Biochemical and biophysical research communications

authors： Zhou X,Jiang G,Zhao A,Bondeva T,Hirszel P,Balla T

更新日期：2001-07-06 00:00:00

abstract：:Ubiquitin (Ub) carboxyl-terminal hydrolase L1 (UCH-L1) has dual functions, such as hydrolase activity on the chemical bonds formed by the C-terminal Gly of Ub and dimerization-dependent ubiquitin ligase activity. Accumulating evidence suggests that dual activities of UCH-L1 were intimately associated with Parkinson's ...

journal_title：Biochemical and biophysical research communications

authors： Park DW,Nam MK,Rhim H

更新日期：2011-11-11 00:00:00

abstract：:Patient-derived xenografts (PDXs) are widely recognised as a more physiologically relevant preclinical model than standard cell lines, but are expensive and low throughput, have low engraftment rate and take a long time to develop. Our newly developed conditional reprogramming (CR) technology addresses many PDX drawba...

journal_title：Biochemical and biophysical research communications

authors： Mondal AM,Ma AH,Li G,Krawczyk E,Yuan R,Lu J,Schlegel R,Stamatakis L,Kowalczyk KJ,Philips GK,Pan CX,Liu X

更新日期：2019-09-10 00:00:00

abstract：:Leigh syndrome (LS) is an incurable, nearly always fatal, neurodegenerative, pediatric disorder that results from respiratory chain failure. The most common mitochondrial DNA (mtDNA) mutations that result in LS are m.8993T→C/G and m.9176T→C/G, which were previously found in several patients with early-onset Leigh synd...

journal_title：Biochemical and biophysical research communications

authors： Ronchi D,Bordoni A,Cosi A,Rizzuti M,Fassone E,Di Fonzo A,Servida M,Sciacco M,Collotta M,Ronzoni M,Lucchini V,Mattioli M,Moggio M,Bresolin N,Corti S,Comi GP

更新日期：2011-08-26 00:00:00

abstract：:The cyclin-dependent kinase (CDK) inhibitor p27(Kip1) is an important regulator of cell cycle progression as it negatively regulates G(0/1) progression and plays a major role in controlling the cell cycle. The screening of the p27(Kip1) sequence identified many potential phosphorylation sites. Although Ser(10) and Thr...

journal_title：Biochemical and biophysical research communications

authors： Guan X,Chen L,Wang J,Geng H,Chu X,Zhang Q,Du L,De W

更新日期：2006-09-01 00:00:00

abstract：:Resveratrol (3,5,4'-trihydroxystilbene) is a naturally occurring phytoalexin, found in grapes and wine, which has been reported to exert a variety of important pharmacological effects. We have investigated the activity of resveratrol on proliferation and differentiation of the promyelocitic cell line HL-60. A concentr...

journal_title：Biochemical and biophysical research communications

authors： Ragione FD,Cucciolla V,Borriello A,Pietra VD,Racioppi L,Soldati G,Manna C,Galletti P,Zappia V

更新日期：1998-09-08 00:00:00

abstract：:Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis ...

journal_title：Biochemical and biophysical research communications

authors： Patarroyo MA,Plaza DF,Ocampo M,Curtidor H,Forero M,Rodriguez LE,Patarroyo ME

更新日期：2008-08-08 00:00:00

abstract：:The fragile X mental retardation 1 (FMR1) protein binds mRNA and acts as a negative regulator of translation. Lack of FMR1 causes the most common neurological disorder, fragile X syndrome, while its overexpression is associated with metastasis of breast cancer. Its activity has been well-studied in nervous tissue, but...

journal_title：Biochemical and biophysical research communications

authors： Choi YN,Jeong DH,Lee JS,Yoo SJ

更新日期：2014-10-10 00:00:00

abstract：:Advances in understanding the neurobiology of addiction indicate that not only dopaminergic neurotransmissions but also glutamatergic neurotransmissions within the mesolimbic system play important roles. While the role for the nucleus accumbens (NAc) shell and core in addiction has been extensively studied, the functi...

journal_title：Biochemical and biophysical research communications

authors： Kim M,Au E,Neve R,Yoon BJ

更新日期：2009-01-30 00:00:00

abstract：:We examined the affinity of transthyretin (TTR) for lipoproteins and the effect of lipoproteins on TTR-related amyloidogenesis using serum samples from healthy volunteers and patients with familial amyloidotic polyneuropathy (FAP) ATTRVal30Met. In both volunteers and patients, TTR levels were highest in the VLDL fract...

journal_title：Biochemical and biophysical research communications

authors： Sun X,Ando Y,Haraoka K,Katsuragi S,Yamashita T,Yamashita S,Okajima M,Terazaki H,Okabe H

更新日期：2003-11-14 00:00:00

abstract：:Elevations in intracellular Ca(2+) ([Ca(2+)](i)) initiate insulin secretion from pancreatic beta-cells, but the secretory responses become rapidly desensitised to maintained elevations in [Ca(2+)](i). We have investigated the mechanisms underlying the Ca(2+) desensitization of insulin secretion using electrically perm...

journal_title：Biochemical and biophysical research communications

authors： Harris TE,Persaud SJ,Squires PE,Jones PM

更新日期：2000-04-21 00:00:00

abstract：:Neurotensin (NT) is now classified as a brain-gut peptide in the central nervous system and gastrointestinal tract. In the present study, we characterized the NT receptors on the rat liver plasma membranes. The specific binding of [3H]NT was time dependent, reversible, and saturable. Scatchard analysis of the specific...

journal_title：Biochemical and biophysical research communications

authors： Muraki K,Nishi Y,Arai M,Kubo N,Ueda K,Shikata H,Nakata Y,Segawa T,Yanaihara N,Yajima H

更新日期：1987-06-30 00:00:00

abstract：BACKGROUND:The activation of hepatic stellate cells plays a central role in the development of liver fibrosis during chronic liver trauma. The aim of the present study was to identify a compound that inhibits the activation process of stellate cells. METHODS:Rat primary cultured stellate cells and a human stellate cel...

journal_title：Biochemical and biophysical research communications

authors： Cui W,Wang M,Maegawa H,Teranishi Y,Kawada N

更新日期：2012-08-31 00:00:00

abstract：:Microbial redox reactions of inorganic sulfur compounds play a vital role in balancing the turnover of this element in the environment. These vital reactions are carried out by the enzyme system encoded by the sox operon. The central player of the sulfur oxidation biochemistry is the SoxY-Z protein complex. Another pr...

journal_title：Biochemical and biophysical research communications

authors： Bagchi A

更新日期：2011-10-22 00:00:00

abstract：:Actin is a highly abundant cytoskeletal protein that is essential for all eukaryotic cells and participates in many structural and functional roles. It has long been noted that estrogen affects cellular morphology. However, recent studies observed that both estrogen and tamoxifen induce a remarkable cytoskeletal remod...

journal_title：Biochemical and biophysical research communications

authors： Wang Z,Sun L,Liang S,Liu ZC,Zhao ZY,Yang J,Wang D,Yang DQ

更新日期：2019-08-27 00:00:00

abstract：:One of the major roles of brain-derived neurotrophic factor (BDNF) is to promote the differentiation and support the survival of neurons in the central nervous system. The objective of the present study was to evaluate the effect of BDNF on the fate of adult rat hippocampus-derived neural stem cells (AHPCs) transplant...

journal_title：Biochemical and biophysical research communications

authors： Suzuki T,Ooto S,Akagi T,Amemiya K,Igarashi R,Mizushima Y,Takahashi M

更新日期：2003-10-03 00:00:00

abstract：:The amino acid sequences in the NH2-terminal region and some other parts of human gastric cathepsin E were investigated. The NH2-terminal sequencing revealed that the cathepsin E preparation which had been activated at pH 4.0 contained one major and one minor isozymes in an approximate molar ratio of 3:1. The NH2-term...

journal_title：Biochemical and biophysical research communications

authors： Athauda SB,Matsuzaki O,Kageyama T,Takahashi K

更新日期：1990-04-30 00:00:00

abstract：:Endothelin-1 is a potent vasoactive peptide which may play a role in the regulation of vascular resistance through its autocrine/paracrine effects. We have investigated the influence of salt loading on the renal and cardiac production of endothelin-1 in stroke prone spontaneously hypertensive rats, a classical model o...

journal_title：Biochemical and biophysical research communications

authors： Feron O,Salomone S,Godfraind T

更新日期：1995-04-06 00:00:00

abstract：:Aberrant expression of cell cycle regulators have been implicated in prostate cancer development and progression. Therefore, understanding transcriptional networks controlling the cell cycle remain a challenge in the development of prostate cancer treatment. In this study, we found that icilin, a super-cooling agent, ...

journal_title：Biochemical and biophysical research communications

authors： Lee S,Chun JN,Kim SH,So I,Jeon JH

更新日期：2013-11-29 00:00:00

abstract：:SUMOylation, a post-translational modification of lysine residues by small ubiquitin-like modifier (SUMO) proteins, has been implicated in the pathogenesis of neurodegenerative disorders including Alzheimer's disease (AD), and in neuron- and astrocyte-specific physiological functions. Global SUMOylation is increased i...

journal_title：Biochemical and biophysical research communications

authors： Maruyama T,Wada H,Abe Y,Niikura T

更新日期：2018-06-02 00:00:00

abstract：:We have established 3T3-L1 cells possessing a secretory Gaussia luciferase (GLuc) gene under the control of nuclear factor-kappa B (NF-κB) response element. The 3T3-L1 cells named 3T3-L1-NF-κB-RE-GLuc could differentiate into adipocyte as comparably as parental 3T3-L1 cells. Inflammatory cytokines such as tumor necros...

journal_title：Biochemical and biophysical research communications

authors： Nagasaki H,Yoshimura T,Aoki N

更新日期：2012-04-13 00:00:00

abstract：:"Activated" glucocorticoid-receptor complexes purified about 3,000-fold from rat liver were found to bind to histone-agarose. Because of their tight binding, they could not be eluted from the column by high salt solution (3 M KCl) or low salt plus polyol buffer (50% ethylene glycol), but their binding could be disrupt...

journal_title：Biochemical and biophysical research communications

authors： Ueda K,Isohashi F,Okamoto K,Kokuhu I,Kimura K,Yoshikawa K,Sakamoto Y

更新日期：1988-03-15 00:00:00

abstract：:The present study aimed to establish a novel efficient nonviral strategy for suicide gene transfer in hepatocellular carcinoma (HCC) in vivo. We employed branched polyethylenimine (PEI) and combined it with Epstein-Barr virus (EBV)-based plasmid vectors. The HCC cells transfected with an EBV-based plasmid carrying the...

journal_title：Biochemical and biophysical research communications

authors： Iwai M,Harada Y,Tanaka S,Muramatsu A,Mori T,Kashima K,Imanishi J,Mazda O

更新日期：2002-02-15 00:00:00

abstract：:By using a radioimmunoassay (RIA) system newly established for human brain natriuretic peptide (BNP), a high concentration of immunoreactive (ir-) human BNP (hBNP) has been found in cardiac atrium (1). Two molecular forms of ir-hBNP of 4K and 13-15K were isolated from atrial extracts by using anti-hBNP IgG immunoaffin...

journal_title：Biochemical and biophysical research communications

authors： Hino J,Tateyama H,Minamino N,Kangawa K,Matsuo H

更新日期：1990-03-16 00:00:00

abstract：:Hepatocellular carcinoma (HCC) tumors evade death in part by downregulating expression of the tumor suppressor gene Interferon regulatory factor-1 (IRF-1). However, the molecular mechanisms accounting for IRF-1 suppression in HCC have not been well described. In this study, we identified a novel microRNA-301a (miR-301...

journal_title：Biochemical and biophysical research communications

authors： Dong K,Du Q,Cui X,Wan P,Kaltenmeier C,Luo J,Yan B,Yan Y,Geller DA

更新日期：2020-04-02 00:00:00

abstract：:AtNPF7.3/AtNRT1.5, which is a nitrate transporter that drives root-to-shoot transport of NO3-, is also involved in modulating the response to K+ deprivation in Arabidopsis by affecting root development and K+ transport. However, whether NPF7.3/NRT1.5 functions in regulating plant responses to deficiencies of other nut...

journal_title：Biochemical and biophysical research communications

authors： Cui YN,Li XT,Yuan JZ,Wang FZ,Wang SM,Ma Q

更新日期：2019-01-01 00:00:00

abstract：:An important mechanism of regulation of the expression of the AT(1) receptors is the modulation of the mRNA stability. AUF1, a human RNA-binding protein, may play an important role. Since AUF1 seems to bind to AU-rich regions of the 3'-untranslated region of the mRNAs, we verified the nucleotide sequence of human AT(1...

journal_title：Biochemical and biophysical research communications

authors： Pende A,Giacchè M,Castigliola L,Contini L,Passerone G,Patrone M,Port JD,Lotti G

更新日期：1999-12-20 00:00:00