Abstract:
:Apolipoprotein (apo-) B mRNA editing at nucleotide 6666 converts cytidine to uridine, transforming the codon for glutamine-2153 to a termination codon. To investigate this editing mechanism, [a-32P] and [5-3H] CTP were incorporated into synthetic apo-B RNA. After the substrate had been edited extensively in vitro by a partially purified editing extract, the edited base was isolated and analyzed for radioactivity. The uridine-6666 resulting from the editing reaction had the same ratio of 3H to 32P as did the cytidine-6666, demonstrating that deamination rather than base exchange or nucleotide replacement is the mechanism for apo-B mRNA editing.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Johnson DF,Poksay KS,Innerarity TLdoi
10.1006/bbrc.1993.2172subject
Has Abstractpub_date
1993-09-30 00:00:00pages
1204-10issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(83)72172-8journal_volume
195pub_type
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