The mechanism for apo-B mRNA editing is deamination.

Abstract:

:Apolipoprotein (apo-) B mRNA editing at nucleotide 6666 converts cytidine to uridine, transforming the codon for glutamine-2153 to a termination codon. To investigate this editing mechanism, [a-32P] and [5-3H] CTP were incorporated into synthetic apo-B RNA. After the substrate had been edited extensively in vitro by a partially purified editing extract, the edited base was isolated and analyzed for radioactivity. The uridine-6666 resulting from the editing reaction had the same ratio of 3H to 32P as did the cytidine-6666, demonstrating that deamination rather than base exchange or nucleotide replacement is the mechanism for apo-B mRNA editing.

authors

Johnson DF,Poksay KS,Innerarity TL

doi

10.1006/bbrc.1993.2172

subject

Has Abstract

pub_date

1993-09-30 00:00:00

pages

1204-10

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(83)72172-8

journal_volume

195

pub_type

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