Visualization of local phosphatidylcholine synthesis within hippocampal neurons using a compartmentalized culture system and imaging mass spectrometry.

abstract：:We have previously shown that a ribozyme directed against human interleukin-6 (IL-6) mRNA is efficient in vivo, despite its poor activity in vitro on full-length IL-6 mRNA. We compared the effect of the nucleocapsid protein of HIV-1 (NCp7) on the ribozyme cleavage reaction of a long (1041 nt) and a short (19 nt) subst...

journal_title：Biochemical and biophysical research communications

authors： Mahieu M,Hendrix C,Ooms J,Herdewijn P,Content J

更新日期：1995-09-05 00:00:00

abstract：:The production of 1 alpha,25-dihydroxyvitamin D3 is known to be down regulated by 1 alpha,25-dihydroxyvitamin D3 itself. It was recently shown that liver mitochondrial sterol 27-hydroxylase CYP27, present also in kidney, catalyzes 1 alpha-hydroxylation of 25-hydroxyvitamin D3. Treatment of vitamin D-deficient rats wit...

journal_title：Biochemical and biophysical research communications

authors： Axén E,Postlind H,Wikvall K

更新日期：1995-10-04 00:00:00

abstract：:Cell division cycle protein 45 (Cdc45) is crucial for the initiation as well as the elongation process of eukaryotic DNA replication. Our findings suggested that the Cdc45 protein is ubiquitylated and degraded by the proteasome pathway in human cells. Firstly, the fate of Cdc45 protein after induction of terminal diff...

journal_title：Biochemical and biophysical research communications

authors： Pollok S,Grosse F

更新日期：2007-11-03 00:00:00

abstract：:Diabetes mellitus (DM) is a risk factor for abnormal heart development, but the molecular mechanism remains obscure. Histone deacetylase 11 (HDAC11), the most recently identified histone deacetylase, is the sole member of class IV HDACs. However, its role in diabetic cardiac injury is still poorly understood. In the p...

journal_title：Biochemical and biophysical research communications

authors： Fan XD,Wan LL,Duan M,Lu S

更新日期：2018-09-05 00:00:00

abstract：:Polychlorinated dibenzo-p-dioxins (PCDDs) are known as g environmental contaminants on account of the extreme toxicity. Among these compounds, 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TetraCDD) is regarded as the most toxic one. The extremely high toxicity of 2,3,7,8-TetraCDD is based on its high affinity for Ah r...

journal_title：Biochemical and biophysical research communications

authors： Shinkyo R,Sakaki T,Takita T,Ohta M,Inouye K

更新日期：2003-08-29 00:00:00

abstract：:Recent evidence suggests that during aging there is widespread accumulation of aggregated insoluble proteins, even in the absence of pathological conditions. Pharmacological manipulation of protein aggregation might be helpful to unveil the involvement of protein aggregates during aging, as well as to develop novel st...

journal_title：Biochemical and biophysical research communications

authors： Cuanalo-Contreras K,Park KW,Mukherjee A,Millán-Pérez Peña L,Soto C

更新日期：2017-01-01 00:00:00

abstract：:Runt-related transcription factor-2 (RUNX2)/core binding factor a1 (Cbfa1) is implicated in the regulation of osteoblast differentiation and osteoblast-specific gene expression. Mutations in RUNX2 cause the bone disease cleidocranial dysplasia, which is characterized by multiple skeletal defects. RUNX2 is expressed as...

journal_title：Biochemical and biophysical research communications

authors： Sudhakar S,Li Y,Katz MS,Elango N

更新日期：2001-11-30 00:00:00

abstract：:Addition of NH4+ to STreptomyces griseus 2682 cells grown in NO3- containing medium resulted in a rapid decline in glutamine synthetase activity due to covalent modification of the enzyme. The NH4+ promoted inactivation of the enzyme was inhibited by the ADP-ribosyltransferase inhibitor 3-methoxybenzamide. In the pres...

journal_title：Biochemical and biophysical research communications

authors： Penyige A,Kálmánczhelyi A,Sipos A,Ensign JC,Barabás G

更新日期：1994-10-28 00:00:00

abstract：:Insulin-like growth factor-II (IGF-II) is a potent mitogen for cells in culture. The H19 gene is a developmentally regulated gene with putative tumor suppressor activity, and loss of H19 expression may be involved in tumorigenesis. The H19 gene is closely linked to the human IGF-II gene (IGF2) on chromosome 11p15.5 an...

journal_title：Biochemical and biophysical research communications

authors： Kiess W,Paquette J,Koepf G,Wolf E,Deal C

更新日期：1999-02-16 00:00:00

abstract：:The catalytic C-C bond cleavage of a lignin model compound was investigated by use of tetraphenylporphyrinatoiron(III)chloride as a model for enzymic degradation of lignin. The C-C bond of the lignin model compound 1,2-bis(4-ethoxy-3-methoxyphenyl) propane-1,3-diol was oxidatively cleaved by catalysis of iron-porphyri...

journal_title：Biochemical and biophysical research communications

authors： Shimada M,Habe T,Umezawa T,Higuchi T,Okamoto T

更新日期：1984-08-16 00:00:00

abstract：:Alpha-L-fucosidase was purified from the bull seminal plasma by chromatography on DEAE-disk, octyl sepharose hydrophobic column and HPLC. The enzyme appeared to be pure as judged by the polyacrylamide gel electrophoresis both under the nondenaturing and denaturing conditions. The pure enzyme promoted the acrosome reac...

journal_title：Biochemical and biophysical research communications

authors： Srivastava PN,Arbtan K,Takei GH,Huang TT,Yanagimachi R

更新日期：1986-06-30 00:00:00

abstract：:We have constructed a series of plasmids containing variations in the DNA that codes for the translation initiation region of preproparathyroid hormone. Gel electrophoretic analyses of the character and extent of synthesis of various modified preproparathyroid hormone like proteins derived from the mRNAs transcribed f...

journal_title：Biochemical and biophysical research communications

authors： Szczesna-Skorupa E,Mead DA,Kemper B

更新日期：1986-10-15 00:00:00

abstract：:Metabolic syndrome describes a group of clinical features that together increase the incidence of coronary artery disease, stroke and type 2 diabetes. Insulin resistance is a major risk factor for developing metabolic syndrome. A chronic state of inflammation accompanies the accumulation of surplus lipids in adipose a...

journal_title：Biochemical and biophysical research communications

authors： Ko SH,Lee JK,Lee HJ,Ye SK,Kim HS,Chung MH

更新日期：2014-01-10 00:00:00

abstract：:Our previous studies demonstrated that intravenous administration of staphylococcal enterotoxin B (SEB) to BALB/c mice resulted in thymocyte apoptosis. In the present study, we evaluated the role of protein kinase C (PKC) in thymocyte apoptosis induced by SEB. Our results showed that the level of protein phosphorylati...

journal_title：Biochemical and biophysical research communications

authors： Lin YS,Kao SF,Jan MS,Cheng ML,Wing LY,Chang WC,Lei HY,Lin MT

更新日期：1995-08-24 00:00:00

abstract：:Cardiac-specific expression of the N1325S mutation of SCN5A in transgenic mouse hearts (TG-NS) resulted in long QT syndrome (LQTS), ventricular arrhythmias (VT), and heart failure. In this study we carried out oligonucleotide mircoarray analysis to identify genes that are differentially expressed in the TG-NS mouse he...

journal_title：Biochemical and biophysical research communications

authors： Wu L,Archacki SR,Zhang T,Wang QK

更新日期：2007-06-29 00:00:00

abstract：:The purpose of this study was to determine the effects of dietary fatty acids on intestinal apolipoprotein (apo) A-IV and C-III expression in the newborn piglet. Two-day-old female piglets received one of three isocaloric formulas containing medium-chain triglycerides (TG) (MCT), intermediate-chain saturated TG (ICST)...

journal_title：Biochemical and biophysical research communications

authors： Black DD,Wang H,Hunter F,Zhan R

更新日期：1996-04-25 00:00:00

abstract：:Very-long-chain acyl-CoA synthetases (VLCS) activate very-long-chain fatty acids (VLCFA) containing 22 or more carbons to their CoA derivatives. We cloned the human ortholog (hVLCS) of the gene encoding the rat liver enzyme (rVLCS). Both hVLCS and rVLCS contain 620 amino acids, are expressed primarily in liver and kid...

journal_title：Biochemical and biophysical research communications

authors： Steinberg SJ,Wang SJ,Kim DG,Mihalik SJ,Watkins PA

更新日期：1999-04-13 00:00:00

abstract：:Amidolysis catalysed by nerve growth factor (7S-NGF) and by a glandular kallikrein, unlike that catalysed by thrombin, was not inhibited by antithrombin III, either in the presence or absence of heparin. Inhibition by alpha 1-proteinase inhibitor of thrombin-, but not of 7S-NGF- or kallikrein-catalysed amidolysis was ...

journal_title：Biochemical and biophysical research communications

authors： Long WF,Williamson FB

更新日期：1983-05-31 00:00:00

abstract：:Acinetobacter lwoffii K24 known as an aniline degrading bacterium has also been found to utilize p-hydroxybenzoate as a sole carbon source. In this study, 2-DE using Q-Sepharose column separation was attempted for fast screening of protocatechuate 3,4-dioxygenase for catabolism of p-hydroxybenzoate in A. lwoffii K24. ...

journal_title：Biochemical and biophysical research communications

authors： Kahng HY,Cho K,Song SY,Kim SJ,Leem SH,Kim SI

更新日期：2002-07-26 00:00:00

abstract：:In order to search for novel estrogen-responsive genes, we performed serial analysis of gene expression (SAGE) for estrogen-treated MCF-7 human breast cancer cells. SAGE analysis of 31,000 and 30,856 tags from non-treated and 17 beta-estradiol (E2)-treated cells for 24 h, respectively, facilitated the identification o...

journal_title：Biochemical and biophysical research communications

authors： Inadera H,Hashimoto S,Dong HY,Suzuki T,Nagai S,Yamashita T,Toyoda N,Matsushima K

更新日期：2000-08-18 00:00:00

abstract：:We have constructed an expression plasmid for regulated expression of the hepatitis B surface antigen gene in yeast using promoter of the yeast Pho5 gene. In the yeast transformants, the monomeric HBsAg (22K dalton) was estimated to constitute approximately 3% of the total proteins. On extraction, the HBsAg was found ...

journal_title：Biochemical and biophysical research communications

authors： Choo KB,Wu SM,Lee HH,Lo SC

更新日期：1985-08-30 00:00:00

abstract：:The effects of a synthetic protein kinase C (PKC) activator, (-)-indolactam V (ILV), were studied in SH-SY5Y human neuroblastoma cells. (-)-ILV induced a translocation of PKC from cytosol to plasma membrane and displaced 3H-phorbol dibutyrate binding in the micromolar range. In addition, (-)-ILV caused a decreased sen...

journal_title：Biochemical and biophysical research communications

authors： Heikkilä J,Akerman KE

更新日期：1989-08-15 00:00:00

abstract：:Monodisperse bovine prothrombin was prepared and its molecular states under several conditions examined. The protein showed no tendency to self-associate in the absence of calcium. Calcium (4 mM) caused small increases in the apparent molecular weight of the protein which may or may not represent protein dimerization ...

journal_title：Biochemical and biophysical research communications

authors： Silversmith RE,Wei GJ,Nelsestuen GL

更新日期：1983-02-28 00:00:00

abstract：:Cisplatin is a potent DNA-damaging agent that has demonstrated anticancer activities against several tumors. However, manifestation of cellular resistance is a major obstacle in anticancer therapy that severely limits the curative potential of cisplatin. Therefore, understanding the molecular basis of cisplatin resist...

journal_title：Biochemical and biophysical research communications

authors： Burger H,Capello A,Schenk PW,Stoter G,Brouwer J,Nooter K

更新日期：2000-03-24 00:00:00

abstract：:Dexamethasone (Dex) induces direct cytotoxicity to cultured osteoblasts. The benzimidazole derivative compound 991 ("C991") is a novel and highly-efficient AMP-activated protein kinase (AMPK) activator. Here, in both MC3T3-E1 osteoblastic cells and primary murine osteoblasts, treatment with C991 activated AMPK signali...

journal_title：Biochemical and biophysical research communications

authors： Xu YY,Chen FL,Ji F,Fei HD,Xie Y,Wang SG

更新日期：2018-01-01 00:00:00

abstract：:Stretch activation of cation-permeable channels may be an important proximal sensory mechanism in mechanotransduction. As actin filaments may mediate cellular responses to changes of the mechanical properties of the substrate and regulate stretch-induced calcium transients, we examined the role of actin filaments and ...

journal_title：Biochemical and biophysical research communications

authors： Wu Z,Wong K,Glogauer M,Ellen RP,McCulloch CA

更新日期：1999-08-02 00:00:00

abstract：:Protein Z was purified from rabbit liver cytosol by affinity chromatography on oleic acid-agarose and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After removal of sodium dodecyl sulfate, the renatured protein was found to bind heme and bilirubin with a Kd of approximately 1 microM which prod...

journal_title：Biochemical and biophysical research communications

authors： Vincent SH,Holeman B,Muller-Eberhard U

更新日期：1985-10-30 00:00:00

abstract：:Osterix (Osx) is a novel zinc finger-containing transcription factor that is essential for osteoblast differentiation and bone formation in bone homeostasis. The mitogen-activated protein (MAP) kinases are a group of evolutionarily conserved proline-directed protein serine/threonine kinases that are activated in respo...

journal_title：Biochemical and biophysical research communications

authors： Choi YH,Gu YM,Oh JW,Lee KY

更新日期：2011-11-25 00:00:00

abstract：:microRNAs have been demonstrated to play important roles in cancer development and progression. Hence, identifying functional microRNAs and better understanding of the underlying molecular mechanisms would provide new clues for the development of targeted cancer therapies. Herein, we reported that a microRNA, miR-421 ...

journal_title：Biochemical and biophysical research communications

authors： Chen L,Tang Y,Wang J,Yan Z,Xu R

更新日期：2013-06-14 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) play crucial roles in tumor development of osteosarcoma (OS). LncRNA PCAT6 was involved in the progression of multiple human cancers. However, the biological function of PCAT6 in OS remains largely unknown. We found that PCAT6 was elevated in OS tissues relative to that in their adjacent ...

journal_title：Biochemical and biophysical research communications

authors： Zhu C,Huang L,Xu F,Li P,Li P,Hu F

更新日期：2020-01-08 00:00:00