当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Interactions of a designed peptide with lipopolysaccharide: Bound conformation and anti-endotoxic activity.

Interactions of a designed peptide with lipopolysaccharide: Bound conformation and anti-endotoxic activity.

Abstract：

:Designed peptides that would selectively interact with lipopolysaccharide (LPS) or endotoxin and fold into specific conformations could serve as important scaffolds toward the development of antisepsis compounds. Here, we describe solution structure of a designed amphipathic peptide, H(2)N-YVKLWRMIKFIR-CONH(2) (YW12D) in complex with endotoxin as determined by transferred nuclear Overhauser effect spectroscopy. The conformation of the isolated peptide is highly flexible, but undergoes a dramatic structural stabilization in the presence of LPS. Structure calculations reveal that the peptide presents two amphipathic surfaces in its bound state to LPS whereby each surface is characterized by two positive charges and a number of aromatic and/or aliphatic residues. ITC data suggests that peptide interacts with two molecules of lipid A. In activity assays, YW12D exhibits neutralization of LPS toxicity with very little hemolysis of red blood cells. Structural and functional properties of YW12D would be applicable in designing low molecular weight non-toxic antisepsis molecules.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Bhunia A,Chua GL,Domadia PN,Warshakoon H,Cromer JR,David SA,Bhattacharjya S

doi

10.1016/j.bbrc.2008.02.105

subject

Has Abstract

pub_date

2008-05-09 00:00:00

pages

853-7

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(08)00384-7

journal_volume

369

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • The focal adhesion kinase amino-terminal domain localises to nuclei and intercellular junctions in HEK 293 and MDCK cells independently of tyrosine 397 and the carboxy-terminal domain.

    abstract：:The function and intracellular localisation of the non-catalytic NH(2)-terminal region of focal adhesion kinase (FAK) are unclear. We investigated the targetting of the FAK NH(2)-terminal domain in HEK 293 and epithelial MDCK cells. Exogenous expression of a variety of GFP-fused and epitope-tagged NH(2) terminal domai...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02547-0

    authors： Stewart A,Ham C,Zachary I

    更新日期：2002-11-22 00:00:00

  • The catalytic mechanism of Escherichia coli aspartate carbamoyltransferase: a molecular modelling study.

    abstract：:Based on molecular modelling study, we propose that the reaction between L-aspartate an carbamoylphosphate, catalyzed by E. coli aspartate carbamoyltransferase, may proceed via a tetrahedral intermediate and that the breakdown of the intermediate is facilitated by an intramolecular proton transfer between the amino gr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91497-5

    authors： Gouaux JE,Krause KL,Lipscomb WN

    更新日期：1987-02-13 00:00:00

  • Inhibition of protein import into mitochondria by amphiphilic cations: potential targets and mechanism of action.

    abstract：:In this paper we describe for the first time the inhibitory effect of three amphiphilic cations, trifluoperazine, propranolol and dibucaine on mitochondrial protein import. The amphiphilic cations did not affect binding of mitochondrial precursor proteins to mitochondria. Import into mitoplasts was affected in a simil...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9590

    authors： Pavlov PF,Glaser E

    更新日期：1998-11-09 00:00:00

  • The role of the N-terminal propeptide of the pro-aminopeptidase processing protease: refolding, processing, and enzyme inhibition.

    abstract：:Pro-aminopeptidase processing protease (PA protease) is an extracellular zinc metalloprotease produced by Aeromonas caviae T-64 and it is classified as M04.016 according to the MEROPS database. The precursor of PA protease consists of four regions; a signal peptide, an N-terminal propeptide, a C-terminal propeptide, a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)00838-0

    authors： Tang B,Nirasawa S,Kitaoka M,Hayashi K

    更新日期：2002-08-09 00:00:00

  • Novel exons located more than 200 kb downstream of the previously described 3' exon of the K-sam gene for generating activated forms of KGF receptor.

    abstract：:The K-sam gene was first identified as an amplified gene in the poorly differentiated types, especially in the scirrhous type, of gastric cancers. We have recently found and reported that the carboxyl-terminal exons of K-sam are frequently deleted in the scirrhous type of gastric cancer. The deletion generates prefere...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1735

    authors： Ueda T,Sasaki H,Aoyagi K,Narikiyo M,Tsubosa Y,Kuwahara Y,Sakamoto H,Mafune K,Yoshida T,Makuuchi M,Terada M

    更新日期：1999-11-30 00:00:00

  • Scallop-derived plasmalogens attenuate the activation of PKCδ associated with the brain inflammation.

    abstract：:Activation of protein kinase C delta (PKCδ) has been linked to the neuroinflammation but the relationship with the various neurodegenerative diseases including the Alzheimer's disease (AD) was mostly elusive. In the AD brains, the special phospholipids, ethanolamine plasmalogens (Pls), were found to be reduced and our...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.06.084

    authors： Sejimo S,Hossain MS,Akashi K

    更新日期：2018-09-05 00:00:00

  • Salt-inducible kinase 2 regulates TFEB and is required for autophagic flux in adipocytes.

    abstract：:Dysregulation of autophagy has been observed in obesity and type 2 diabetes. Salt-inducible kinase 2 (SIK2), a member of the AMPK-related kinase family, is downregulated in adipocytes from obese or insulin resistant individuals and was previously demonstrated to regulate autophagy in cancer and normal cell lines. The ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.11.177

    authors： Negoita F,Säll J,Morén B,Stenkula K,Göransson O

    更新日期：2019-01-15 00:00:00

  • Ochratoxin A decreases the activity of phosphoenolpyruvate carboxykinase and its mRNA content in primary cultures of rat kidney proximal convoluted tubule cells.

    abstract：:The effect of ochratoxin A on the specific activities of phosphoenolpyruvate carboxykinase, gamma-glutamyl transpeptidase and phosphate-dependent glutaminase were investigated in primary cultures of rat kidney proximal convoluted tubule cells grown in a serum-free medium supplemented with growth factors. Addition of o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)90759-6

    authors： Thekkumkara TJ,Patel MS

    更新日期：1989-08-15 00:00:00

  • Quinone reduction by Rhodothermus marinus succinate:menaquinone oxidoreductase is not stimulated by the membrane potential.

    abstract：:Succinate:quinone oxidoreductase (SQR), a di-haem enzyme purified from Rhodothermus marinus, reveals an HQNO-sensitive succinate:quinone oxidoreductase activity with several menaquinone analogues as electron acceptors that decreases with lowering the redox midpoint potential of the quinones. A turnover with the low-po...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.015

    authors： Fernandes AS,Konstantinov AA,Teixeira M,Pereira MM

    更新日期：2005-05-06 00:00:00

  • Identification of human plasma C1 inhibitor as a target protein for staphylococcal superantigen-like protein 5 (SSL5).

    abstract：:The family of staphylococcal superantigen-like proteins (SSLs) have a structure similar to bacterial superantigens but exhibit no superantigenic activity. These exoproteins have recently been shown to disturb the host immune defense system. One family member, SSL5, was reported to bind to human leukocyte P-selectin gl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.12.026

    authors： Oku T,Kurisaka C,Ando Y,Tsuji T

    更新日期：2019-01-22 00:00:00

  • MicroRNA-140 suppresses osteosarcoma tumor growth by enhancing anti-tumor immune response and blocking mTOR signaling.

    abstract：:Immune checkpoint blockade is proved to be a promising therapeutic strategy against several human malignancies. MicroRNAs (miRNAs) are ∼22 nucleotide RNAs that play important roles in regulating gene expression, either suppressing its translation or speeding up its degradation. In this study, we demonstrated that miR-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.11.120

    authors： Ji X,Wang E,Tian F

    更新日期：2018-01-01 00:00:00

  • Inhibition of the infectivity and cytopathic effect of human immunodeficiency virus by water-soluble lignin in an extract of the culture medium of Lentinus edodes mycelia (LEM).

    abstract：:Inhibition of the infectivity and cytopathic effect of human immunodeficiency virus type 1 (HIV-1) by the immunoactive fractions obtained from LEM, which is an extract of the culture medium of Lentinus edodes mycelia, is reported. A purified fraction, EPS4, obtained from LEM by ethanol precipitation followed by hydrop...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91665-3

    authors： Suzuki H,Okubo A,Yamazaki S,Suzuki K,Mitsuya H,Toda S

    更新日期：1989-04-14 00:00:00

  • Adipose tissue-deprived stem cells acquire cementoblast features treated with dental follicle cell conditioned medium containing dentin non-collagenous proteins in vitro.

    abstract：:Adipose tissue-derived stem cells (ADSCs), which are easily harvested and show excellent pluripotency potential, have generated considerable interest in regenerative medicine. In this study, the differentiation of ADSCs was assessed after treatment with dental follicle cell conditioned medium (DFCCM) containing dentin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.05.067

    authors： Wen X,Nie X,Zhang L,Liu L,Deng M

    更新日期：2011-06-10 00:00:00

  • Stimulation of Ca2+ release-activated Ca2+ channels as a potential mechanism involved in non-genomic 1,25(OH)2-vitamin D3-induced Ca2+ entry in skeletal muscle cells.

    abstract：:As in other vitamin D target cells, activation of voltage-dependent Ca2+ channels (VDCC) mediates the fast, non-genomic, 1,25(OH)2D3 stimulation of Ca2+ influx in skeletal muscle cells (SMC). 1,25(OH)2D3 has also been shown to rapidly induce the release of Ins(1,4,5)P3 in SMC. Experiments were performed to investigate...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7501

    authors： Vazquez G,de Boland AR,Boland R

    更新日期：1997-10-20 00:00:00

  • Calumenin, a multiple EF-hands Ca2+-binding protein, interacts with ryanodine receptor-1 in rabbit skeletal sarcoplasmic reticulum.

    abstract：:Calumenin is a multiple EF-hand Ca2+-binding protein located in endo/sarcoplasmic reticulum of mammalian tissues. In the present study, we cloned two rabbit calumenin isoforms (rabbit calumenin-1 and -2, GenBank Accession Nos. SY225335 and AY225336, respectively) by RT-PCR. Both isoforms contain a 19 aa N-terminal sig...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.02.115

    authors： Jung DH,Mo SH,Kim DH

    更新日期：2006-04-28 00:00:00

  • Different roles of β-arrestin and the PKA pathway in mitochondrial ROS production induced by acute β-adrenergic receptor stimulation in neonatal mouse cardiomyocytes.

    abstract：:Reactive oxygen species (ROS) play a crucial role in various physiological and pathological processes mediated by β-adrenergic receptors (β-ARs) in cardiomyocytes. However, the sources and signaling pathways involved in ROS production induced by acute β-AR activation have not yet been fully defined. In primary neonata...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.05.140

    authors： Zhang J,Xiao H,Shen J,Wang N,Zhang Y

    更新日期：2017-08-05 00:00:00

  • Interaction of Trypanosoma cruzi with cells with altered glycosylation patterns.

    abstract：:Glycosylation mutants of chinese hamster ovary cells were used to analyse the role played by surface-exposed carbohydrates on the process of interaction of Trypanosoma cruzi with the host cell. Adhesion and invasion of the parasites were markedly reduced in cells which express very few sialic acid residues. Infection ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1684

    authors： Ciavaglia Mdo C,de Carvalho TU,de Souza W

    更新日期：1993-06-15 00:00:00

  • Fibroblast growth factor 2 positively regulates expression of activating transcription factor 4 in osteoblasts.

    abstract：:Our previous studies showed that basic fibroblast growth factor 2 (FGF2) null mice display markedly reduced bone mass and bone formation. However, the mechanism by which FGF2 regulates bone mass or bone formation is not fully defined. Activating transcription factor 4 (ATF4), one member of activating transcription fac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.11.059

    authors： Fei Y,Xiao L,Hurley MM

    更新日期：2010-01-01 00:00:00

  • Regulation of stretch-activated intracellular calcium transients by actin filaments.

    abstract：:Stretch activation of cation-permeable channels may be an important proximal sensory mechanism in mechanotransduction. As actin filaments may mediate cellular responses to changes of the mechanical properties of the substrate and regulate stretch-induced calcium transients, we examined the role of actin filaments and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1057

    authors： Wu Z,Wong K,Glogauer M,Ellen RP,McCulloch CA

    更新日期：1999-08-02 00:00:00

  • Silkworm hemolymph as a potent inhibitor of apoptosis in Sf9 cells.

    abstract：:We have previously shown that silkworm hemolymph exhibits anti-apoptotic activity against baculovirus-induced Sf9 cell apoptosis. In this study, using various chemical inducers, such as actinomycin D, camptothecin, and staurosporine, we found that silkworm hemolymph inhibits insect cell apoptosis induced not only by b...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)00746-5

    authors： Rhee WJ,Kim EJ,Park TH

    更新日期：2002-07-26 00:00:00

  • Effects of a protein kinase C inhibitor, K-252a, on human polymorphonuclear neutrophil responsiveness.

    abstract：:We investigated the capacity of K-252a, an inhibitor of rat brain protein kinase C (PKC), to influence polymorphonuclear neutrophil (PMN) PKC and PMN activation with chemically and structurally dissimilar agonists. K-252a inhibited PMN PKC (IC50 = 0.58 microM), and caused a concentration-dependent (0.1-10 microM) inhi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80455-8

    authors： Smith RJ,Justen JM,Sam LM

    更新日期：1988-05-16 00:00:00

  • A protective role of 27-kDa heat shock protein in glucocorticoid-evoked apoptotic cell death of hippocampal progenitor cells.

    abstract：:Hippocampus is one of the most vulnerable tissues to glucocorticoid (GC). In the present study, we demonstrate that dexamethasone (DEX), a synthetic GC, induces apoptotic cell death in hippocampal progenitor HiB5 cells without any additional insult. Interestingly, expression of 27-kDa heat shock protein (HSP27) was ma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.10.152

    authors： Son GH,Geum D,Chung S,Park E,Lee KH,Choi S,Kim K

    更新日期：2005-12-30 00:00:00

  • PICOT binding to the polycomb group protein, EED, alters H3K27 methylation at the MYT1 PRC2 target gene.

    abstract：:PICOT is a ubiquitous protein that has no functional redundant ortholog and is critical for mouse embryonic development. It is involved in the regulation of signal transduction in T lymphocytes and cardiac muscle, and in cellular iron metabolism and biogenesis of Fe/S proteins. However, very little is known about the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.12.153

    authors： Pandya P,Pasvolsky R,Babichev Y,Braiman A,Witte S,Altman A,Isakov N

    更新日期：2019-02-05 00:00:00

  • PES1 regulates sensitivity of colorectal cancer cells to anticancer drugs.

    abstract：:PES1 (also known as Pescadillo), a nucleolar protein, was involved in biogenesis of ribosomal RNA. Up-regulation of PES1 has been documented in some human cancers, indicating that PES1 may play some crucial roles in tumorigenesis. In our previous study, it was found that silencing of PES1 resulted in decreased prolife...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.12.145

    authors： Xie W,Qu L,Meng L,Liu C,Wu J,Shou C

    更新日期：2013-02-15 00:00:00

  • Sequencing and characterization of the human thiazide-sensitive Na-Cl cotransporter (SLC12A3) gene promoter.

    abstract：:The thiazide-sensitive Na-Cl cotransporter SLC12A3 displays expression restricted to distal convoluted tubule cells where it catalyzes the uptake of sodium and chloride through the apical membrane. We sequenced 1959 bp of the 5' flanking region of human SLC12A3, located the area of transcription initiation, and used d...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4673

    authors： MacKenzie S,Vaitkevicius H,Lockette W

    更新日期：2001-04-13 00:00:00

  • Function of P-glycoprotein expressed in placenta and mole.

    abstract：:We examined the expression of P-glycoprotein in human placentas and hydatidiform moles. Trophoblasts in all the examined placentas and moles expressed P-glycoprotein, and the size of the P-glycoprotein was smaller than that in multidrug-resistant human epidermoid carcinoma KB cells. The P-glycoprotein in the placenta ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6855

    authors： Nakamura Y,Ikeda S,Furukawa T,Sumizawa T,Tani A,Akiyama S,Nagata Y

    更新日期：1997-06-27 00:00:00

  • Nucleotide sequence of a cDNA clone for human aldolase: a messenger RNA in the liver.

    abstract：:Nearly complete cDNA clones for human aldolase A mRNA were isolated from human liver cDNA library and the nucleotide sequence determined. Using the cDNA clone as a probe the length of human aldolase A mRNAs, isolated from the skeletal muscle, liver and placenta tissues, was measured by RNA blotting and estimated to be...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91818-2

    authors： Sakakibara M,Mukai T,Hori K

    更新日期：1985-08-30 00:00:00

  • Interactions in the active site of Deinococcus radiodurans RNA polymerase during RNA proofreading.

    abstract：:Co-transcriptional RNA proofreading by RNA polymerase (RNAP) is essential for accurate mRNA synthesis and reactivation of stalled transcription complexes, which can otherwise compromise genome integrity. RNAP from the stress-resistant bacterium Deinococcus radiodurans exhibits high levels of RNA cleavage in comparison...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.12.095

    authors： Esyunina D,Kulbachinskiy A

    更新日期：2019-01-29 00:00:00

  • Effects of osmotic stress and hyperglycemia on aldose reductase gene expression in human renal proximal tubule cells.

    abstract：:Sorbitol levels in proximal tubule cells cultured for 96 h in the presence of 16.5 mM and 27.5 mM glucose were significantly elevated when compared to cells cultured in 5.5 mM glucose. No changes in the levels of aldose reductase activity, mRNA and immunoreactivity were observed in cells cultured for up to 168 h in me...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81479-2

    authors： Petrash JM,Flath M,Sens D,Bylander J

    更新日期：1992-08-31 00:00:00

  • Kinetin delays the onset of ageing characteristics in human fibroblasts.

    abstract：:Kinetin (Kn) is a synthetic cytokinin plant growth hormone having some senescence-retarding effects in plants. Its effects on animal cells have been, until now, best considered as growth inhibitory and anti-tumorigenic. However, we have observed that an addition of 40-200 microM Kn in the culture medium of human diplo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1752

    authors： Rattan SI,Clark BF

    更新日期：1994-06-15 00:00:00