Abstract:
:The monoclonal antibody 6E2 raised against the embryonal carcinoma cell line NCR-G3 had been shown to also react with human germ cells. Thin-layer chromatography (TLC) immunostaining revealed that 6E2 specifically reacts with sialosylglobopenta osylceramide (sialylGb5), which carries an epitope of stage-specific embryonic antigen-4 (SSEA-4), known as an important cell surface marker of embryogenesis. The immunostaining of mouse preimplantation embryos without fixation showed that the binding of 6E2 caused the clustering and consequent accumulation of sialylGb5 at the interface between blastomeres. These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between blastomeres after binding of 6E2.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Sato B,Katagiri YU,Miyado K,Akutsu H,Miyagawa Y,Horiuchi Y,Nakajima H,Okita H,Umezawa A,Hata J,Fujimoto J,Toshimori K,Kiyokawa Ndoi
10.1016/j.bbrc.2007.10.093subject
Has Abstractpub_date
2007-12-28 00:00:00pages
838-43issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(07)02243-7journal_volume
364pub_type
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