Amylase secretion from isolated pure acinar cells.

abstract：:Serum leptin levels are elevated in subjects with exogenous obesity, indicating that obesity is associated with leptin resistance. Since in man no abnormalities have yet been found in either the genes for leptin or its receptor, the mechanism of leptin resistance in obesity remains unknown. To determine if resistance ...

journal_title：Biochemical and biophysical research communications

authors： Diamond FB Jr,Eichler DC,Duckett G,Jorgensen EV,Shulman D,Root AW

更新日期：1997-04-28 00:00:00

abstract：:The splicing of 3.36kb mouse mitochondrial mRNA precursor(L-strand) to 69bp RNA component is shown to be mediated by an endoribonuclease activity associated with Lettre Ehrlich ascites tumor cell mitochondria. The enzymic activity was partially purified by column chromatography and the in vitro reconstituted RNA proce...

journal_title：Biochemical and biophysical research communications

authors： Bhattacharya P

更新日期：1993-10-15 00:00:00

abstract：:Leukotrienes play an important pathophysiological role in chronic inflammatory states and, as previously shown, cause increased levels of cyclooxygenase-2 (COX-2) in intestinal epithelial cells. The anti-apoptotic protein Bcl-2 is also elevated by LTD(4) stimulation, and in colon cancer, so we studied the mechanisms o...

journal_title：Biochemical and biophysical research communications

authors： Wikström K,Ohd JF,Sjölander A

更新日期：2003-03-07 00:00:00

abstract：:Mutations in the breast cancer susceptibility gene (BRCA1) account for a significant proportion of hereditary breast and ovarian cancers. Cofactor of BRCA1 (COBRA1) was isolated as a BRCA1-interacting protein and exhibited a similar chromatin reorganizing activity to that of BRCA1. However, the biological role of COBR...

journal_title：Biochemical and biophysical research communications

authors： Zhong H,Zhu J,Zhang H,Ding L,Sun Y,Huang C,Ye Q

更新日期：2004-12-10 00:00:00

abstract：:Dehaloperoxidase (DHP) from Amphitrite ornata is the only heme-containing, hydrogen peroxide-dependent globin capable of oxidatively dehalogenating halophenols to yield the corresponding quinones. To ascertain that this enzymatic activity is intrinsic to DHP, we have cloned and expressed the enzyme in Escherichia coli...

journal_title：Biochemical and biophysical research communications

authors： Osborne RL,Taylor LO,Han KP,Ely B,Dawson JH

更新日期：2004-11-26 00:00:00

abstract：:Cultured human endothelial cells produce increased levels of PA-I when stimulated with IL-1. The stimulatory effect was elicited by both alpha (pI 5) and beta (pI 7) IL-1 molecules. The effect was both dose and time-dependent, plateau reached after 8 h. The PA-I measured in cell extracts was SDS-resistant with an appa...

journal_title：Biochemical and biophysical research communications

authors： Grames M,Breviario F,Pintucci G,Millet I,Dejana E,van Damme J,Donati MB,Mussoni L

更新日期：1986-09-14 00:00:00

abstract：:A hitherto unknown synergism exerted by retinol (vitamin A) and L-ascorbic acid (vitamin C) was discovered using endothelial cells. Retinol stimulated the extracellular and intracellular activities of plasminogen activator up to approximately 8- and 4-fold from the control values, respectively. L-Ascorbic acid enhance...

journal_title：Biochemical and biophysical research communications

authors： Inada Y,Hagiwara H,Kojima S,Shimonaka M,Saito Y

更新日期：1985-07-16 00:00:00

abstract：:Exploring the molecular mechanisms that regulate the osteogenesis of human mesenchymal stem cells (hMSCs) will bring us more efficient methods for improving the treatment of bone-related diseases. In this study, we analyzed the effects of miR-31 on the osteogenesis of hMSCs. The overexpression of miR-31 repressed the ...

journal_title：Biochemical and biophysical research communications

authors： Xie Q,Wang Z,Bi X,Zhou H,Wang Y,Gu P,Fan X

更新日期：2014-03-28 00:00:00

abstract：:A neuropeptide was isolated from the corpora cardiaca of the corn earworm moth Heliothis zea, and purified by sequential gradient elution in three reversed phase-high performance liquid chromatographic steps. The primary structure, pGlu-Leu-Thr-Phe-Ser-Ser-Gly-Trp-Gly-Asn-NH2 was determined by automated gas-phase Edma...

journal_title：Biochemical and biophysical research communications

authors： Jaffe H,Raina AK,Riley CT,Fraser BA,Bird TG,Tseng CM,Zhang YS,Hayes DK

更新日期：1988-08-30 00:00:00

abstract：:In mammalian cells, the Rab7 protein is a key element of late endocytic membrane traffic. Several results suggest that it is involved in the transport from early to late endosome or from late endosome to lysosome. We have previously characterized a Rab7 gene homologue (TcRAB7) in Trypanosoma cruzi. Now, using an affin...

journal_title：Biochemical and biophysical research communications

authors： Araripe JR,Cunha e Silva NL,Leal ST,de Souza W,Rondinelli E

更新日期：2004-08-20 00:00:00

abstract：:The immunosuppressant FK506 inhibits thymocyte positive selection. Calcineurin, a FK506-sensitive Ca2+/calmodulin-dependent protein phosphatase, is presumed to be involved in this event without direct evidence. We have previously shown that moderate stimulation of CD4(+)CD8(+) thymocytes with a combination of the calc...

journal_title：Biochemical and biophysical research communications

authors： Kuwata T,Asada A,Ohoka Y,Mukai M,Miyaike M,Iwata M

更新日期：1998-06-18 00:00:00

abstract：:Activated Kupffer cells and hepatic macrophages can produce massive liver necrosis through microcirculatory disturbance due to sinusoidal fibrin deposition. This mechanism is involved in the development of liver injury after endotoxin administration in rats pretreated with heat-killed Propionibacterium acnes (P.acnes)...

journal_title：Biochemical and biophysical research communications

authors： Toshima K,Mochida S,Ishikawa K,Matsui A,Arai M,Ogata I,Fujiwara K

更新日期：1998-05-29 00:00:00

abstract：:Enzymes belonging to the S9 family of prolyl oligopeptidases are of interest because of their pharmacological importance and have a non-catalytic β-propeller domain. In this study, we found that the oxidation of Met203, which lies on surface of the β-propeller domain, leads to change in the substrate specificity of er...

journal_title：Biochemical and biophysical research communications

authors： Tokai S,Bito T,Shimizu K,Arima J

更新日期：2017-05-27 00:00:00

abstract：:The main protein of enriched and purified amyloid from Octodon degus pancreatic islets was identified as insulin. The material was reduced and alkylated and the A- and the B-chain were separated by reversed phase chromatography and subjected to Edman degradation and amino acid analysis. It was shown that the A-chain c...

journal_title：Biochemical and biophysical research communications

authors： Hellman U,Wernstedt C,Westermark P,O'Brien TD,Rathbun WB,Johnson KH

更新日期：1990-06-15 00:00:00

abstract：:Liver kinase B1 (LKB1), a tumour suppressor, participates in many cellular processes, including cell survival, growth, apoptosis, transformation, and metabolism. Upon performing yeast two-hybrid screening, co-immunoprecipitation, and GST pull-down, we identified that BRCA1-associated protein 1 (BAP1), a deubiquitinase...

journal_title：Biochemical and biophysical research communications

authors： Yang C,Ding H,Yang Y,Yang L,Yang Y,Fang M,Ren J,Hu R,Wang C,Geng W

更新日期：2020-09-03 00:00:00

abstract：:Two proteins (25- and 22-kDa) that cross-react with anti-gizzard SM22 alpha antibody were isolated from bovine aorta. The former, identical to a SM22 alpha homolog reported previously (Kobayashi, R., Kubota, T., and Hidaka, H. (1994) Biochem. Biophys. Res. Commun. 198, 1275-1280), associates with the membrane fraction...

journal_title：Biochemical and biophysical research communications

authors： Shishibori T,Yamashita K,Bandoh J,Oyama Y,Kobayashi R

更新日期：1996-12-04 00:00:00

abstract：:S100A8 and S100A9 are members of the S100A8 protein family that exist as homodimers and heterodimers in neutrophils, monocytes, and macrophages. Recent studies have shown the pivotal roles of S100A8 and S100A9 in the propagation of inflammation and keratinocyte proliferation in psoriasis. We found significant up-regul...

journal_title：Biochemical and biophysical research communications

authors： Lee Y,Jang S,Min JK,Lee K,Sohn KC,Lim JS,Im M,Lee HE,Seo YJ,Kim CD,Lee JH

更新日期：2012-07-13 00:00:00

abstract：:The envelope glycoprotein E2 is the major immunodominant protein of the classical swine fever virus and can induce neutralizing antibodies and protective host-immune responses in infected swine. We designed, expressed, and purified multi-epitope protein (GST-BT22) that contains a tandem repeat of the E2 antigenic-dete...

journal_title：Biochemical and biophysical research communications

authors： Tian H,Hou X,Liu X

更新日期：2013-02-08 00:00:00

abstract：:A polyclonal antiserum raised against purified alpha-agglutinin was made specific for alpha-agglutinin after adsorption with a cells. The adsorbed antiserum identified alpha-agglutinin peptides on Western blots and bound to cell surface alpha-agglutinin, inhibiting the binding of alpha cells to a cells. Using the anti...

journal_title：Biochemical and biophysical research communications

authors： Wojciechowicz D,Lipke PN

更新日期：1989-05-30 00:00:00

abstract：:The expression of mouse CYP27B1 in Escherichia coli has been dramatically enhanced by coexpression of GroEL/ES. To reveal the enzymatic properties of CYP27B1, we measured its hydroxylation activity toward vitamin D3 and 1alpha-hydroxyvitamin D3 (1alpha(OH)D3) in addition to the physiological substrate 25(OH)D3. Surpri...

journal_title：Biochemical and biophysical research communications

authors： Uchida E,Kagawa N,Sakaki T,Urushino N,Sawada N,Kamakura M,Ohta M,Kato S,Inouye K

更新日期：2004-10-15 00:00:00

abstract：:Arrestins have important roles in G protein-coupled receptor (GPCR) signaling including desensitization of GPCRs and G protein-independent signaling. There have been four arrestins identified: arrestin1, arrestin2 (e.g. β-arrestin1), arrestin3 (e.g. β-arrestin2), and arrestin4. β-Arrestin1 and β-arrestin2 are ubiquito...

journal_title：Biochemical and biophysical research communications

authors： Yun Y,Kim DK,Seo MD,Kim KM,Chung KY

更新日期：2015-01-30 00:00:00

abstract：:A hammerhead ribozyme directed against murine TNFalpha (mTNFalpha) mRNA has been constructed. In vitro studies showed that this ribozyme was released from the parent molecule by flanking cis-acting hammerhead and hairpin ribozymes. This same anti-mTNFalpha ribozyme specifically cleaved both synthetically derived subst...

journal_title：Biochemical and biophysical research communications

authors： MacKay SL,Tannahill CL,Auffenberg T,Ksontini R,Copeland EM 3rd,Moldawer LL

更新日期：1999-07-05 00:00:00

abstract：:The pathway employed for pentachlorophenol (PCP) degradation by an aerobic, chlorophenol-utilizing Flavobacterium sp. was initiated by conversion of PCP to tetrachloro-p-hydroquinone (TCH). 18O labelling experiments demonstrated that the first dechlorination, where a hydroxyl replaced the chlorine at PCP ring position...

journal_title：Biochemical and biophysical research communications

authors： Steiert JG,Crawford RL

更新日期：1986-12-15 00:00:00

abstract：:We questioned whether glucocorticoids alter the known potent effect of serum to increase glucose transport and GLUT-1 expression in cultured L6 myotubes. Myotubes were exposed to serum-free medium for 24h in the presence or absence of 300 nM dexamethasone and subsequently treated for 8h with 10% serum or serum free me...

journal_title：Biochemical and biophysical research communications

authors： Sivitz WI,Pasley AM

更新日期：1995-01-05 00:00:00

abstract：:Oxidants and inflammatory mediators such as tumour necrosis factor-alpha (TNF-alpha) activate transcription factors such as NF-kappa B. Interleukin-8 (IL-8) is a ubiquitous inflammatory chemokine that mediates a multitude of inflammatory events in the lung. Ergothioneine is a naturally occurring thiol compound, which ...

journal_title：Biochemical and biophysical research communications

authors： Rahman I,Gilmour PS,Jimenez LA,Biswas SK,Antonicelli F,Aruoma OI

更新日期：2003-03-21 00:00:00

abstract：:Cultured human lymphocytes and fibroblasts accumulate methotrexate during 24 hours and synthesize methotrexate polyglutamates up to the hexaglutamate, with the triglutamate predominating. In the interval after incubation with methotrexate, drug is lost, metabolites are converted to longer chain-lengths, and methotrexa...

journal_title：Biochemical and biophysical research communications

authors： Whitehead VM,Kalman TI,Vuchich MJ

更新日期：1987-04-14 00:00:00

abstract：:Monoclonal antibody BB1 was previously shown to recognize the CD80 protein as well as a putative B7.3 molecule, both ligands of the important T-cell receptors CD28 and CTLA-4. We report that BB1 coprecipitated from detergent lysates of human B- and pre-B-cell lines a protein kinase activity, as detected by solid phase...

journal_title：Biochemical and biophysical research communications

authors： Angelisová P,Cerný J,Horejsí V

更新日期：1996-11-12 00:00:00

abstract：:CPI-17 is a protein phosphatase 1 (PP1) inhibitor that has been shown to act on the myosin light chain phosphatase. CPI-17 is phosphorylated on Thr-38 in vivo, thus enhancing its ability to inhibit PP1. Thr-38 has been shown to be the target of several protein kinases in vitro. Originally, the expression of CPI-17 was...

journal_title：Biochemical and biophysical research communications

authors： Dubois T,Howell S,Zemlickova E,Learmonth M,Cronshaw A,Aitken A

更新日期：2003-03-07 00:00:00

abstract：:A novel inhibitory receptor of immunoglobin superfamily (IgSF), IgSF member 13 (IgSF13), has been identified from human dendritic cells (DC). IgSF13 is a type I transmembrane protein containing an N-terminal signal peptide, a extracellular region with a single Ig V-like domain, a transmembrane region, and a cytoplasmi...

journal_title：Biochemical and biophysical research communications

authors： Sui L,Li N,Liu Q,Zhang W,Wan T,Wang B,Luo K,Sun H,Cao X

更新日期：2004-07-02 00:00:00

abstract：:Brewers yeast contains factors that increase and decrease glucose tolerance. Hop components (lupulones) that adhere to yeast during the brewing process elicit a variety of biological effects including the induction of hepatic cytochrome P4503A. Colupulone was tested for its effects on glucose tolerance and cytochrome ...

journal_title：Biochemical and biophysical research communications

authors： Mannering GJ,Shoeman JA,Shoeman DW

更新日期：1994-05-16 00:00:00