Alpha 2-antiplasmin's carboxy-terminal lysine residue is a major site of interaction with plasmin.

abstract：:We prepared three peptide fragments (fg.59-105, fg.63-105 and fg.64-105) by the BrCN cleavage of mutant lysozymes where Ile58, Trp62 and Trp63 were mutated to Met, respectively. From the analysis of formation of the disulfide bonds among Cys64, Cys76, Cys80 and Cys94 in the renaturation of each peptide fragment from t...

journal_title：Biochemical and biophysical research communications

authors： Ueda T,Ohkuri T,Imoto T

更新日期：1996-11-01 00:00:00

abstract：:Plasma membrane fractions from mouse livers were examined for the presence of growth regulatory peptides. Both growth inhibitory and growth stimulatory factors were found on these membranes. The growth inhibitory component could be enriched by extractions with both dimethylmaleic anhydride and octylglucoside. The grow...

journal_title：Biochemical and biophysical research communications

authors： Lieberman MA

更新日期：1984-05-16 00:00:00

abstract：:Peptidylarginine deiminases (PADs) convert arginine residues in proteins into citrulline residues Ca(2+)-dependently. PAD V was recently found in granulocyte-differentiated HL-60 cells. To find a target of PAD V, we incubated HL-60 granulocytes with the calcium ionophore A23187 and studied deiminated proteins by immun...

journal_title：Biochemical and biophysical research communications

authors： Hagiwara T,Nakashima K,Hirano H,Senshu T,Yamada M

更新日期：2002-01-25 00:00:00

abstract：:The present study has focused on the role of the 42- and 44-kDa mitogen-activated protein kinases (p42/44 MAPKs) and the 38-kDa mitogen-activated protein kinase (p38 MAPK) in the proliferation of the pancreatic beta-cell line MIN6. MIN6 beta-cell proliferation was assessed by measuring 5-bromo-2'-deoxyuridine (BrdU) i...

journal_title：Biochemical and biophysical research communications

authors： Burns CJ,Squires PE,Persaud SJ

更新日期：2000-02-16 00:00:00

abstract：:Hepatocytes isolated from normal adult rats were maintained at sub-confluent density in a defined medium in primary culture. In control cultures with added epidermal growth factor and low concentrations of dexamethasone, the rate of DNA synthesis was initially low but increased after about 30 hours in culture. Additio...

journal_title：Biochemical and biophysical research communications

authors： Edwards AM,Lucas CM

更新日期：1985-08-30 00:00:00

abstract：:Several protein quality control systems in bacteria and/or mitochondrial matrix from lower eukaryotes are absent in higher eukaryotes. These are transfer-messenger RNA (tmRNA), The N-end rule ATP-dependent protease ClpAP, and two more ATP-dependent proteases, HslUV and ClpXP (in yeast). The lost proteases resemble the...

journal_title：Biochemical and biophysical research communications

authors： Lehmann G,Ziv T,Braten O,Admon A,Udasin RG,Ciechanover A

更新日期：2016-06-17 00:00:00

abstract：:Mutations in vacuolar protein sorting 13A (VPS13A) gene are responsible for chorea-acanthocytosis (ChAc). We previously determined the full-length sequence and exon-intron structure of mouse VPS13A and generated a ChAc model mouse by using the gene targeting technique. In the process, we found diverse 5' and 3' transc...

journal_title：Biochemical and biophysical research communications

authors： Mizuno E,Nakamura M,Agemura A,Kusumoto A,Ichiba M,Kurano Y,Muroya S,Sano A

更新日期：2007-02-23 00:00:00

abstract：:Calreticulin isolated from spinach leaves has been specifically phosphorylated in vitro by protein kinase CK2 while animal calreticulin from rabbit liver is not a substrate of this kinase under the same conditions. Phosphoserine is the only phosphoamino acid detected. High affinity binding (Km = 4.4 microM) and a near...

journal_title：Biochemical and biophysical research communications

authors： Baldan B,Navazio L,Friso A,Mariani P,Meggio F

更新日期：1996-04-25 00:00:00

abstract：:An antifungal protein that inhibits the growth of filamentous fungal pathogens was isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) by affinity chromatography on Affi-gel blue gel and ion exchange chromatography on CM-Sepharose. The N-terminal amino acid sequence of the protein was highly homolog...

journal_title：Biochemical and biophysical research communications

authors： Lee JR,Park SC,Kim JY,Lee SS,Park Y,Cheong GW,Hahm KS,Lee SY

更新日期：2007-02-16 00:00:00

abstract：:Alanine:glyoxylate aminotransferase has been reported to be present as the apo form in the peroxisomes and as the holo form in the mitochondria in chicken kidney. In contrast, the enzyme was found to be present as the holo form both in the peroxisomes and in the mitochondria in pigeon kidney, suggesting that birds are...

journal_title：Biochemical and biophysical research communications

authors： Sakuraba H,Fujiwara S,Noguchi T

更新日期：1991-05-15 00:00:00

abstract：:The peptide deformylase in bacteria is involved in removal of N-formyl group from newly synthesized proteins. The gene encoding this enzyme from Mycobacterium tuberculosis was cloned and expressed in Escherichia coli. The enzyme activity of the recombinant protein (mPDF) was insensitive to modulation by common monoval...

journal_title：Biochemical and biophysical research communications

authors： Saxena R,Chakraborti PK

更新日期：2005-07-01 00:00:00

abstract：:The peptide endothelin causes a biphasic rise in intracellular free calcium levels in cultured Type-1 astrocytes and C6 glioma cells, suggesting that glial cells may be the physiological target of endothelin in the brain. Endothelin also causes a calcium-dependent increase [3H]thymidine incorporation in primary cultur...

journal_title：Biochemical and biophysical research communications

authors： Supattapone S,Simpson AW,Ashley CC

更新日期：1989-12-29 00:00:00

abstract：:Heme is central to oxygen sensing and utilization in all living organisms. It directly regulates numerous molecular and cellular processes for systems that sense or use oxygen. In mammals, heme plays an indispensable role in erythroid cell differentiation. To investigate heme regulatory functions, we identified, by di...

journal_title：Biochemical and biophysical research communications

authors： Zhu Y,Hon T,Zhang L

更新日期：1999-04-29 00:00:00

abstract：:The lectin wheat germ agglutinin (WGA) elicited a prompt and sharp increase in intracellular Ca2+ concentration in human platelets. The WGA-induced Ca2+ mobilization was markedly inhibited by a protein kinase inhibitor staurosporine, whereas Ca2+ mobilization by receptor-mediated agonists, including thrombin, platelet...

journal_title：Biochemical and biophysical research communications

authors： Yatomi Y,Ozaki Y,Koike Y,Satoh K,Kume S

更新日期：1993-03-15 00:00:00

abstract：:Recent studies have demonstrated that adult cells such as pancreatic exocrine cells can be converted to pancreatic β-cells in a process called cell reprogramming. Enteroendocrine cells and β-cells share similar pathways of differentiation during embryonic development. Notably, enteroendocrine K cells express many of t...

journal_title：Biochemical and biophysical research communications

authors： Lee E,Ryu GR,Moon SD,Ko SH,Ahn YB,Song KH

更新日期：2014-01-17 00:00:00

abstract：:Inflammatory processes within the cornea are known to be associated with corneal neovascularization (CN). We examined the effects of inflammatory mediators on the expression of angiogenic factors by corneal cells. TNF-alpha and IL-1 induced VEGF-A secretion by corneal fibroblasts (HCRF) and this was inhibited signific...

journal_title：Biochemical and biophysical research communications

authors： Kommineni VK,Nagineni CN,William A,Detrick B,Hooks JJ

更新日期：2008-09-26 00:00:00

abstract：:The relationship between gene expression and the patterns of histone methylation in Drosophila cells has been investigated using inhibitors of transcription acting at various levels. Inhibition of ribosomal RNA synthesis and processing by 5-fluorouridine or of general RNA synthesis by camptothecin, an inhibitor of top...

journal_title：Biochemical and biophysical research communications

authors： Desrosiers R,Tanguay RM

更新日期：1989-08-15 00:00:00

abstract：:Although mTOR (mammalian target of rapamycin) activation is frequently observed in acute myeloid leukemia (AML) patients, the precise function and the downstream targets of mTOR are poorly understood. Here we revealed that PFKFB3, but not PFKFB1, PFKFB2 nor PFKFB4 was a novel downstream substrate of mTOR signaling pat...

journal_title：Biochemical and biophysical research communications

authors： Feng Y,Wu L

更新日期：2017-02-05 00:00:00

abstract：:A focal freeze injury to rat cerebral cortex induces an early (less than 5 min) increase in brain ornithine decarboxylase activity and an accumulation of polyamines involving cerebral microvessels. This polyamine synthesis correlates with the abnormal increase in microvascular permeability, monitored by uptake of Evan...

journal_title：Biochemical and biophysical research communications

authors： Koenig H,Goldstone AD,Lu CY

更新日期：1983-11-15 00:00:00

abstract：:Two fragments of the E. coli rrnB ribosomal RNA P1 promoter Upstream Activation Region exhibit a constant gel retardation, over a polyacrylamide gel concentration range of 3% to 10%. Gel retardation is usually seen to increase with polyacrylamide gel concentration as in the case of the 219 base pair Crithidia fascicul...

journal_title：Biochemical and biophysical research communications

authors： Wheeler D

更新日期：1993-05-28 00:00:00

abstract：:Using small interfering RNA (siRNA) to induce sequence specific gene silencing is fast becoming a standard tool in functional genomics. As siRNAs in some cases tolerate mismatches with the mRNA target, knockdown of genes other than the intended target could make results difficult to interpret. In an investigation of 3...

journal_title：Biochemical and biophysical research communications

authors： Snøve O Jr,Holen T

更新日期：2004-06-18 00:00:00

abstract：:This present study aims at establishing a novel in vivo gene delivery system for intra-articular tissues. Plasmid DNA (pDNA) carrying the firefly luciferase or enhanced green fluorescent protein (EGFP) genes as markers was injected into a joint space and electric stimuli were given percutaneously with a pair of electr...

journal_title：Biochemical and biophysical research communications

authors： Ohashi S,Kubo T,Kishida T,Ikeda T,Takahashi K,Arai Y,Terauchi R,Asada H,Imanishi J,Mazda O

更新日期：2002-05-24 00:00:00

abstract：:Sirolimus (rapamycin), a new immunosuppressive drug, inhibits proliferation of a wide spectrum of T and B cells. The immunosuppressive mechanism of sirolimus is still unclear. We recently isolated a membrane associated protein with an apparent molecular weight of 210 kDa, p210, from cultured Molt 4 cells and BJAB cell...

journal_title：Biochemical and biophysical research communications

authors： Chen Y,Chen H,Rhoad AE,Warner L,Caggiano TJ,Failli A,Zhang H,Hsiao CL,Nakanishi K,Molnar-Kimber KL

更新日期：1994-08-30 00:00:00

abstract：:A family of short interspersed repetitive elements (SINEs), designated mermaid, is present in the genomes of fish, amphibian and primates, but absent in the mouse genome. We have demonstrated that the sequences of the mermaid family are highly polymorphic in the zebrafish genome as in the human genome. We have also sh...

journal_title：Biochemical and biophysical research communications

authors： Shimoda N,Chevrette M,Ekker M,Kikuchi Y,Hotta Y,Okamoto H

更新日期：1996-03-07 00:00:00

abstract：:Conjugated linoleic acid (CLA), a group of minor fatty acids from ruminant origin, has long been recognized as a body fat lowering agent. Given the trans(t)10,cis(c)12-CLA well documented interference on lipolysis, we hypothesized for adipocytes altered permeation to glycerol when supplemented with this isomer. 3T3-L1...

journal_title：Biochemical and biophysical research communications

authors： Martins SV,Madeira A,Lopes PA,Pires VM,Alfaia CM,Prates JA,Moura T,Soveral G

更新日期：2015-03-06 00:00:00

abstract：:We examined the contribution of apoptosis- and oxidative stress-associated genes to apoptosis induction in trophoblast cells of human fetal membrane tissues undergoing apoptosis during in vitro incubation. RT-PCR analyses demonstrated an increased level of HO-1, Mn-SOD, Cox-2, iNOS, TNFalpha, TNFR1, IL-1beta, IL-6, Ba...

journal_title：Biochemical and biophysical research communications

authors： Yuan B,Ohyama K,Bessho T,Toyoda H

更新日期：2006-03-17 00:00:00

abstract：:The complete amino acid sequence of the cysteine proteinase inhibitor cystatin B (formerly named CPI-B) from human liver was determined. The 98-residue sequence (Mr = 11,175) was obtained by automated solid-phase Edman degradation of a large cyanogen bromide fragment and peptides generated by enzymatic cleavage. The p...

journal_title：Biochemical and biophysical research communications

authors： Ritonja A,Machleidt W,Barrett AJ

更新日期：1985-09-30 00:00:00

abstract：:The synthesis of sufficient amounts of oligosaccharides is the bottleneck for the study of their biological function and their possible use as drug. As an alternative for chemical synthesis, we propose to use Escherichia coli as a "living factory." We have addressed the production of the Galp alpha(1-3)Galp beta(1-4)G...

journal_title：Biochemical and biophysical research communications

authors： Bettler E,Imberty A,Priem B,Chazalet V,Heyraud A,Joziasse DH,Geremia RA

更新日期：2003-03-14 00:00:00

abstract：:In order to search for novel estrogen-responsive genes, we performed serial analysis of gene expression (SAGE) for estrogen-treated MCF-7 human breast cancer cells. SAGE analysis of 31,000 and 30,856 tags from non-treated and 17 beta-estradiol (E2)-treated cells for 24 h, respectively, facilitated the identification o...

journal_title：Biochemical and biophysical research communications

authors： Inadera H,Hashimoto S,Dong HY,Suzuki T,Nagai S,Yamashita T,Toyoda N,Matsushima K

更新日期：2000-08-18 00:00:00

abstract：:Thyroid cells transformed by the Kirsten-ras oncogene become tumorigenic in syngeneic animals. Their growth is no longer dependent on TSH but becomes dependent on serum. Combining morphological and biochemical evidence, we show that serum withdrawal induces apoptotic cell death in Kirsten and Harvey-ras transformed th...

journal_title：Biochemical and biophysical research communications

authors： di Jeso B,Ulianich L,Racioppi L,D'Armiento F,Feliciello A,Pacifico F,Consiglio E,Formisano S

更新日期：1995-09-25 00:00:00