The H159A mutant of yeast enolase 1 has significant activity.

abstract：:The cell membrane receptor for epidermal growth factor (EGF) appears to be a glycoprotein of Mr 170,000 and mediates the mitogenic and metabolic responses of cells with EGF receptors (EGF-R). Normal rat kidney (NRK) have about 3 X 10(5) EGF-R per cell. Upon transformation of NRK cells by Kirsten sarcoma virus, the tra...

journal_title：Biochemical and biophysical research communications

authors： Chua CC,Geiman DE,Schreiber AB,Ladda RL

更新日期：1984-01-30 00:00:00

abstract：:Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear DNA binding protein that participates in processes involving nicking and resealing DNA strands. A genomically unstable subpopulation of PARP-1(-/-) cells has recently been described, which disappears after stable transfection of the cells with complete PARP-1 cDNA. H...

journal_title：Biochemical and biophysical research communications

authors： Cayuela ML,Carrillo A,Ramirez P,Parrilla P,Yélamos J

更新日期：2001-07-13 00:00:00

abstract：:The collagenase from the larvae Hypoderma lineatum is a serine proteinase sequentially related to the trypsin family. The tryptic peptide containing the serine residue of the active site, labelled with [3H] diisopropylfluorophosphate was isolated and determined to be Ser-Pro-Cys-Phe-Gly-Asp-Ser-Gly-Gly-Pro-(Phe-Ser)-L...

journal_title：Biochemical and biophysical research communications

authors： Lecroisey A,Keil B

更新日期：1983-05-16 00:00:00

abstract：:Abnormal perpetual inflammatory response and sequential cytokine-induced prostaglandin E2 (PGE2) play important roles in the pathogenesis of rheumatoid arthritis (RA). The underlying regulatory mechanism, however, remain largely unknown. Here, we discovered that expression level of Metastasis associated protein 1 (MTA...

journal_title：Biochemical and biophysical research communications

authors： Wang H,Dong BW,Zheng ZH,Wu ZB,Li W,Ding J

更新日期：2016-04-29 00:00:00

abstract：:Heparin binding neurite-promoting factor (HBNF) is a highly basic 136 amino acid protein containing 10 cysteine residues. We have determined the redox status and the disulfide arrangement of the cysteine residues in HBNF from bovine brain and refolded human recombinant protein produced in E. coli. Our data indicate th...

journal_title：Biochemical and biophysical research communications

authors： Hulmes JD,Seddon AP,Decker MM,Böhlen P

更新日期：1993-04-30 00:00:00

abstract：:The clottable protein (CP) was isolated from white shrimp, Penaeus vannamei plasma as a 400-kDa protein that splits to two identical 200-kDa subunits when it is reduced with 2-ME. However, using DTT as reducing agent, four main bands were observed; two of them (179 and 125 kDa) had the same N-terminus sequence of the ...

journal_title：Biochemical and biophysical research communications

authors： Reyes-Izquierdo T,Vargas-Albores F

更新日期：2001-09-21 00:00:00

abstract：:alpha-Difluoromethylornithine (DFMO) has been widely used for determining the amounts of ornithine decarboxylase (ODC) in cells. However, we have now found that DFMO does not react with ODC-antizyme complex, indicating that the amounts of radioactive DFMO bound to the protein may not reflect the amounts of ODC in crud...

journal_title：Biochemical and biophysical research communications

authors： Kitani T,Fujisawa H

更新日期：1986-06-30 00:00:00

abstract：:The tailspike protein (TSP) of bacteriophage P22 is a homotrimeric multifunctional protein responsible for recognition and hydrolysis of Salmonella typhimurium host receptors. Once properly folded, TSP shows an unusual stability to temperature and detergent denaturation, prompting the analysis of TSP as a framework fo...

journal_title：Biochemical and biophysical research communications

authors： Carbonell X,Villaverde A

更新日期：1998-03-17 00:00:00

abstract：:Maternally inherited diabetes and deafness (MIDD) is a mitochondrial syndrome characterized by the onset of sensorineural hearing loss and diabetes in adults. Some patients may have other additional clinical features common in mitochondrial disorders such as pigmentary retinopathy, ptosis, cardiomyopathy, myopathy and...

journal_title：Biochemical and biophysical research communications

authors： Mezghani N,Mnif M,Mkaouar-Rebai E,Kallel N,Charfi N,Abid M,Fakhfakh F

更新日期：2013-02-22 00:00:00

abstract：:As vectors, adenoviruses (Ads) have many attractive advantages for in vivo gene therapy. However, Ads do not usually integrate into the host genome and gene expression is, thus, transient. Adeno-associated virus (AAV) integrates into a specific locus (AAVS1) on the human host's chromosome 19, while conventional recomb...

journal_title：Biochemical and biophysical research communications

authors： Ueno T,Matsumura H,Tanaka K,Iwasaki T,Ueno M,Fujinaga K,Asada K,Kato I

更新日期：2000-07-05 00:00:00

abstract：:Comparative fluorimetric, flow cytofluorimetric and fluorescence ratiometric determinations of intracellular K+ concentrations in murine haemopoietic cells (FDCP-Mix clone A4) cultured in the presence and absence of the specific growth factor Interleukin-3 were carried out with the fluorescent potassium-binding benzof...

journal_title：Biochemical and biophysical research communications

authors： Laskay G,Várhelyi T,Dale RE,Dexter TM

更新日期：1995-09-14 00:00:00

abstract：:Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane cytokine and a potent inducer of apoptosis. Epidermal growth factor (EGF) signaling is well known to involve in tumor survival and overexpression of EGF receptor (EGF-R) attributes to decreased responsiveness to many avail...

journal_title：Biochemical and biophysical research communications

authors： Park SY,Seol DW

更新日期：2002-07-12 00:00:00

abstract：:The expression of the transcription factor Sp1 and EGF receptor (EGFR) was examined on human gastric carcinoma cell lines and gastric carcinoma tissues as well as corresponding normal mucosa in order to clarify the mechanism of overexpression of EGFR gene without gene amplification. All 7 carcinoma cell lines expresse...

journal_title：Biochemical and biophysical research communications

authors： Kitadai Y,Yasui W,Yokozaki H,Kuniyasu H,Haruma K,Kajiyama G,Tahara E

更新日期：1992-12-30 00:00:00

abstract：:Different groups including ours have shown that curcumin induces melanoma cell apoptosis, here we focused the role of mammalian Sterile 20-like kinase 1 (MST1) in it. We observed that curcumin activated MST1-dependent apoptosis in cultured melanoma cells. MST1 silencing by RNA interference (RNAi) suppressed curcumin-i...

journal_title：Biochemical and biophysical research communications

authors： Yu T,Ji J,Guo YL

更新日期：2013-11-08 00:00:00

abstract：:The disruption of intestinal mucosal immune tolerance can lead to the development of intestinal immune diseases such as food allergy (FA). Regulatory T cells (Tregs) in the mucosa play a critical role in maintaining peripheral immune tolerance in the intestine, and retinoic acid (RA) is absolutely required for the ind...

journal_title：Biochemical and biophysical research communications

authors： Yamamoto T,Matsunami E,Komori K,Hayashi S,Kadowaki M

更新日期：2019-08-27 00:00:00

abstract：:Using a monospecific antibody to the major cytosolic glutathione-S-transferase of human liver, we have isolated a cDNA clone from a human liver cDNA expression vector library in lambda gt11. The clone cross-hybridizes with a rat liver ligandin (glutathione-S-transferase 1-2) cDNA probe. The clone has an insert of 1.25...

journal_title：Biochemical and biophysical research communications

authors： Butera L,Monnier JR,Campbell E,Bhargava MM

更新日期：1987-02-13 00:00:00

abstract：:Glutathione S-transferase Y-2 mRNA synthesis was induced in yeast Issatchenkia orientalis approximately 37-fold by cultivation with o-dinitrobenzene. A cDNA library complementary to poly (A)+RNA of I. orientalis grown with o-dinitrobenzene was screened by colony hybridization. Twenty positive clones were obtained from...

journal_title：Biochemical and biophysical research communications

authors： Tamaki H,Kumagai H,Tochikura T

更新日期：1990-10-30 00:00:00

abstract：:In this study, we examined the endothelin (ET) receptor subtype involved in mitogenic signaling in human primary and metastatic melanoma cell lines. In a reverse transcriptase-polymerase chain reaction (RT-PCR) study, ET(B) mRNA expression in metastatic melanoma cells was decreased from that of primary melanoma. Only ...

journal_title：Biochemical and biophysical research communications

authors： Kikuchi K,Nakagawa H,Kadono T,Etoh T,Byers HR,Mihm MC,Tamaki K

更新日期：1996-02-27 00:00:00

abstract：:Dopamine D4 receptor (DRD4) has received attention in terms of pathogenesis of schizophrenia and association with human personalities. We isolated the human DRD4 gene containing the 5'-flanking region and determined its sequence. Analysis of the DRD4 transcripts by 5'-RACE (5'-rapid amplification of cDNA ends) reveale...

journal_title：Biochemical and biophysical research communications

authors： Kamakura S,Iwaki A,Matsumoto M,Fukumaki Y

更新日期：1997-06-18 00:00:00

abstract：:Fibrinogen deposition and smooth muscle cell migration are important causes of atherosclerosis and angiogenesis. Involvement of calpains in vascular smooth muscle cell adhesion onto fibrinogen was investigated. Using calpain inhibitors, we showed that activation of calpains was required for smooth muscle cell spreadin...

journal_title：Biochemical and biophysical research communications

authors： Paulhe F,Bogyo A,Chap H,Perret B,Racaud-Sultan C

更新日期：2001-11-09 00:00:00

abstract：:Inorganic pyrophosphate can function as phosphate donor in protein phosphorylation reactions in yeast mitochondria. It was shown that, when PPi substitutes for ATP as inhibitor of the pyruvate dehydrogenase reaction, maximal activity is reached after a lag-period of 30-60 minutes. 32P-labeling of peptides shows that [...

journal_title：Biochemical and biophysical research communications

authors： da Silva LP,Lindahl M,Lundin M,Baltscheffsky H

更新日期：1991-08-15 00:00:00

abstract：:RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a large family of functionally distinct proteins. We describe the cloning and characterization of a novel human transcript RNF38 encoding a new member of the RING finger protein family. The complete mRNA consists of about 6.8 kb widely expr...

journal_title：Biochemical and biophysical research communications

authors： Eisenberg I,Hochner H,Levi T,Yelin R,Kahan T,Mitrani-Rosenbaum S

更新日期：2002-06-28 00:00:00

abstract：:Mitofusin1 (Mfn1) mediates outer mitochondrial membrane (OMM) fusion in Opisthokonts. The uncharacterized TM comprises to two helices (namely, the TM1 and TM2) connected by an intermembrane loop. Consistent with previous studies, our results from in silico analyses show that all mitofusins lack N terminal-MTS and the ...

journal_title：Biochemical and biophysical research communications

authors： Sinha S,Aradhyam GK

更新日期：2019-02-12 00:00:00

abstract：:Hypothetical lambda protein ORF314 shows significant homology with the carboxyl end of phage T4 tail-fiber protein gp37. Homology can also be demonstrated between hypothetical lambda protein ORF194 and a fragment of bacteriophage T4 protein gp38. This sequence homology is also reflected in the genomic sequences of the...

journal_title：Biochemical and biophysical research communications

authors： George DG,Yeh LS,Barker WC

更新日期：1983-09-30 00:00:00

abstract：:The mechanisms underlying diabetes-mediated bone loss are not well defined. It has been reported that the advanced glycation endproducts (AGEs) and receptor for AGEs (RAGEs) are involved in diabetic complications. Here, mice deficient in RAGE were used as a model for investigating the effects of RAGE on bone mass. We ...

journal_title：Biochemical and biophysical research communications

authors： Ding KH,Wang ZZ,Hamrick MW,Deng ZB,Zhou L,Kang B,Yan SL,She JX,Stern DM,Isales CM,Mi QS

更新日期：2006-02-24 00:00:00

abstract：:Interleukin 6 is a pleiotropic cytokine produced in the central nervous system (CNS) that has been involved in both direct neurotrophic activities and in the regulation of the production of acute phase proteins both at peripheral and central levels. In rat cortical type I astrocytes, interleukin 6 release is under the...

journal_title：Biochemical and biophysical research communications

authors： Grimaldi M,Florio T,Schettini G

更新日期：1997-06-09 00:00:00

abstract：:In this report, we demonstrate that HL-60 nuclei isolated in calcium but not EGTA containing buffers specifically bind PE and express approximately 37,000 receptor sites/nucleus. Nuclear phorbol ester binding is lost by isolation in the absence of calcium, but can be repleted by the addition of partially purified prot...

journal_title：Biochemical and biophysical research communications

authors： Kraft AS,Appling C,Berkow RL

更新日期：1987-04-14 00:00:00

abstract：:The effect of tyrosine-phosphorylation on the conformation of three tyrosine-based immunoreceptor activation motifs, zeta(69-86), zeta(106-126), and zeta(138-155), located in the T cell receptor/CD3 zeta-chain was investigated. Circular dichroism and Fourier-transform infrared spectroscopy of the nonphosphorylated and...

journal_title：Biochemical and biophysical research communications

authors： Laczkó I,Hollósi M,Vass E,Hegedüs Z,Monostori E,Tóth GK

更新日期：1998-01-26 00:00:00

abstract：:In this study, we assessed the profile of cell proliferation, Na,K-ATPase activity and apoptosis in primary cultures of proximal tubule cells. Freshly isolated proximal tubules exhibited apoptosis very early in culture: floated cells within 6 hr; attached tubule cells within 20 hr. The onset of apoptosis in either att...

journal_title：Biochemical and biophysical research communications

authors： Tang MJ,Cheng YR,Lin HH

更新日期：1996-01-26 00:00:00

abstract：:Structural determinants of L-[(3)H]nicotine binding to synthetic peptides comprising residues 188-207 of nicotinic acetylcholine receptor alpha subunits were invesitigated by equilibrium binding analysis. Two binding components were detected, one of low affinity (K(d) approximately 1.5 microM) that did not differ sign...

journal_title：Biochemical and biophysical research communications

authors： Lentz TL

更新日期：2000-02-16 00:00:00