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Glutathione S-transferase in yeast: induction of mRNA, cDNA cloning and expression in Escherichia coli.

Abstract：

:Glutathione S-transferase Y-2 mRNA synthesis was induced in yeast Issatchenkia orientalis approximately 37-fold by cultivation with o-dinitrobenzene. A cDNA library complementary to poly (A)+RNA of I. orientalis grown with o-dinitrobenzene was screened by colony hybridization. Twenty positive clones were obtained from 6,000 clones and seven of twenty positive clones expressed glutathione S-transferase activity in E. coli. One of the expressing clones harboring plasmid pHT108 had 28 times more glutathione S-transferase activity induced by Isopropyl-beta-D-thio-galactopyranoside than a strain harboring plasmid pUC118. Expressed glutathione S-transferase Y-2 protein comigrated with yeast glutathione S-transferase Y-2 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as detected by immunoblot analysis.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Tamaki H,Kumagai H,Tochikura T

doi

10.1016/0006-291x(90)90726-4

subject

Has Abstract

pub_date

1990-10-30 00:00:00

pages

669-75

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(90)90726-4

journal_volume

172

pub_type

杂志文章

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