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Antibodies reveal the cytolocalization and subunit structure of the 1,4-dihydropyridine component of the neuronal Ca2+ channel.

Abstract：

:Rabbit brain synaptosomes bind the 1,4-dihydropyridine derivative (+)[3H]-PN 200-110 with an equilibrium dissociation constant of 0.04 nM and a maximal binding capacity of 400 fmol/mg of protein. Using polyclonal antibodies raised against the different components of the skeletal muscle 1,4-dihydropyridine receptor, we have demonstrated that the brain and muscle receptors share the same subunit composition comprising a large polypeptide chain of Mr 140,000 associated by disulfide bridges with a smaller peptide of Mr 32,000. These antibodies have been used in immunofluorescence staining of brain sections. They reveal a distribution of the Ca2+ channel protein similar to that of 1,4-dihydropyridine binding sites with (+)[3H]PN 200-110 by the autoradiographic technique.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Schmid A,Barhanin J,Mourre C,Coppola T,Borsotto M,Lazdunski M

doi

10.1016/s0006-291x(86)80276-5

subject

Has Abstract

pub_date

1986-09-30 00:00:00

pages

996-1002

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(86)80276-5

journal_volume

139

pub_type

杂志文章

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