A form of human basic fibroblast growth factor with an extended amino terminus.

abstract：:The MoFe protein of the complex metalloenzyme nitrogenase folds as a heterotetramer containing two copies each of the homologous alpha and beta subunits, encoded by the nifD and the nifK genes respectively. Recently, the functional expression of a fusion NifD-K protein of nitrogenase was demonstrated in Azotobacter vi...

journal_title：Biochemical and biophysical research communications

authors： Lahiri S,Pulakat L,Gavini N

更新日期：2005-11-18 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARgamma), a member of nuclear receptors, is expressed at a high level in adipose tissue and plays an important role in adipocyte differentiation. In the present study, we identified the expression of PPARgamma in rat aortic smooth muscle cells (RASMC) using reverse t...

journal_title：Biochemical and biophysical research communications

authors： Iijima K,Yoshizumi M,Ako J,Eto M,Kim S,Hashimoto M,Sugimoto N,Liang YQ,Sudoh N,Toba K,Ouchi Y

更新日期：1998-06-18 00:00:00

abstract：:The cell number as well as the hexokinase and glucokinase activity of liver parenchymal and nonparenchymal cells were studied in methapyrilene treated rats. The number of nonparenchymal cells was doubled after treatment with methapyrilene for two weeks while that of hepatocytes remained constant. The hexokinase activi...

journal_title：Biochemical and biophysical research communications

authors： Fischer W,Wagle SR,Katz NR

更新日期：1983-09-30 00:00:00

abstract：:We have cloned and functionally expressed a sodium-dependent human nucleoside transporter, hCNT2, from a CNS cancer cell line U251. Our cDNA clone of hCNT2 had the same predicted amino acid sequence as the previously cloned hCNT2 transporter. Of the several cell lines studied, the best hCNT2 transport function was obt...

journal_title：Biochemical and biophysical research communications

authors： Shin HC,Landowski CP,Sun D,Vig BS,Kim I,Mittal S,Lane M,Rosania G,Drach JC,Amidon GL

更新日期：2003-08-01 00:00:00

abstract：:Recent studies have established that polypeptide growth factors cause an elevation of the cytoplasmic pH (pHi) in cultured mammalian cells by stimulating Na+/H+ exchange. We show that vanadate, previously found to act as a mitogen for a number of cells, reversibly activates Na+/H+ exchange at micromolar concentrations...

journal_title：Biochemical and biophysical research communications

authors： Cassel D,Zhuang YX,Glaser L

更新日期：1984-01-30 00:00:00

abstract：:A synthetic heptapeptide H-Ser-Phe-Phe-Leu-Arg-Asn-Pro-NH2, which corresponds to the ligand peptide latent in rodent thrombin receptors, was able to activate the thrombin receptor with no thrombin. In order to evaluate the structural requisites of two consecutive phenylalanines, three sets of analogs with substitution...

journal_title：Biochemical and biophysical research communications

authors： Shimohigashi Y,Nose T,Okazaki M,Satoh Y,Ohno M,Costa T,Shimizu N,Ogino Y

更新日期：1994-08-30 00:00:00

abstract：:Trilobolide and 2,5-di(tert-butyl)-1,4-benzohydroquinone (BHQ) are potent inhibitors of the Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum. Desoxytrilobolide and 2,5-di(tert-butyl)-1,4-diacetylphenol (acetyl-BHQ) have much lower potencies than their parent compounds and 2,5-di(tert-butyl)-1,4-benzoquinone (BQ...

journal_title：Biochemical and biophysical research communications

authors： Wictome M,Holub M,East JM,Lee AG

更新日期：1994-03-15 00:00:00

abstract：:Chitin oligosaccharides elicited chitinase activity in melon plants. Hexamer to nonamer were the most efficient elicitors: hexamer for maximal stimulation of colorimetrically assessed chitinase activity, heptamer for maximal stimulation of radiochemically assessed chitinase activity. Chitinase elicitation was a rapid ...

journal_title：Biochemical and biophysical research communications

authors： Roby D,Gadelle A,Toppan A

更新日期：1987-03-30 00:00:00

abstract：:Opioid binding properties of Tyr-D-Ser-Gly-Phe-Leu-Thr-NH-NH-Gly-Mal (DSLET-Mal), a novel enkephalin-framed affinity label, was determined in rat brain membranes. In competition studies the ligand showed high affinity for the delta opioid sites, labelled by [(3)H][Ile(5,6)]deltorphin II (K(i) = 8 nM), whereas its bind...

journal_title：Biochemical and biophysical research communications

authors： Szatmári I,Orosz G,Medzihradszky K,Borsodi A

更新日期：1999-11-19 00:00:00

abstract：:Circadian and photic regulation of mitogen-activated protein kinase (MAPK) has been shown to associate closely with the function of the circadian clock in vertebrate clock tissues such as the mouse suprachiasmatic nucleus (SCN). Here we show that, in the central region of the mouse SCN, MAPK exhibited circadian and da...

journal_title：Biochemical and biophysical research communications

authors： Nakaya M,Sanada K,Fukada Y

更新日期：2003-06-06 00:00:00

abstract：:Marked increase in protein levels of Mn-superoxide dismutase (Mn-SOD) was found in TNF-resistant cell lines after treatment with Tumor Necrosis Factor (TNF). No such increase was observed in Cu,Zn-superoxide dismutase (Cu, Zn-SOD) protein in either TNF-resistant or sensitive cells. These results support the data that ...

journal_title：Biochemical and biophysical research communications

authors： Kawaguchi T,Takeyasu A,Matsunobu K,Uda T,Ishizawa M,Suzuki K,Nishiura T,Ishikawa M,Taniguchi N

更新日期：1990-09-28 00:00:00

abstract：:We have isolated and sequenced a cDNA encoding the human luteinizing hormone--choriogonadotropin (LH/hCG) receptor. The deduced amino acid sequence (699 residues) containing seven putative transmembrane segments displays sequence similarity to G protein-coupled receptors. The receptor consists of 335 residue extracell...

journal_title：Biochemical and biophysical research communications

authors： Minegishi T,Nakamura K,Takakura Y,Miyamoto K,Hasegawa Y,Ibuki Y,Igarashi M,Minegish T corrected to Minegishi T

更新日期：1990-11-15 00:00:00

abstract：:The cardiac transcription factor GATA4 is essential for cardiac development, and mutations in this gene have been implicated in a wide variety of congenital heart diseases in both animal models and humans. However, whether mutated GATA4 predisposes to dilated cardiomyopathy (DCM) remains unknown. In this study, the wh...

journal_title：Biochemical and biophysical research communications

authors： Li RG,Li L,Qiu XB,Yuan F,Xu L,Li X,Xu YJ,Jiang WF,Jiang JQ,Liu X,Fang WY,Zhang M,Peng LY,Qu XK,Yang YQ

更新日期：2013-10-04 00:00:00

abstract：:VHL tumor suppressor protein contains two domains, alpha and beta. The alpha-domain is involved in the formation of a large protein complex suggested to be involved in ubiquitin-mediated protein degradation. However, the role of the beta-domain, which may recognize the target proteins for protein degradation, remains ...

journal_title：Biochemical and biophysical research communications

authors： Okuda H,Hirai S,Takaki Y,Kamada M,Baba M,Sakai N,Kishida T,Kaneko S,Yao M,Ohno S,Shuin T

更新日期：1999-09-24 00:00:00

abstract：:Homeobox gene expression in osteoblast-like cells was investigated using the polymerase chain reaction (PCR). A total of 13 homeobox genes was detected in U-2 OS (human osteosarcoma) and MC3T3-E1 (mouse osteoblast) cells by sequencing cloned PCR products. Using specific primers, a different pattern of Hox gene express...

journal_title：Biochemical and biophysical research communications

authors： Kloen P,Visker MH,Olijve W,van Zoelen EJ,Boersma CJ

更新日期：1997-04-17 00:00:00

abstract：:We examined the affinity of transthyretin (TTR) for lipoproteins and the effect of lipoproteins on TTR-related amyloidogenesis using serum samples from healthy volunteers and patients with familial amyloidotic polyneuropathy (FAP) ATTRVal30Met. In both volunteers and patients, TTR levels were highest in the VLDL fract...

journal_title：Biochemical and biophysical research communications

authors： Sun X,Ando Y,Haraoka K,Katsuragi S,Yamashita T,Yamashita S,Okajima M,Terazaki H,Okabe H

更新日期：2003-11-14 00:00:00

abstract：:Our previous study demonstrated that angiotensin II (Ang II) upregulates the expression of Kv1.5, a promising target for atrial fibrillation (AF) therapy, by activating ROS-dependent P-Smad2/3 and P-ERK 1/2. A recent study showed that hydrogen sulfide (H2S) may modulate the effects of angiotensin II (Ang II) by inhibi...

journal_title：Biochemical and biophysical research communications

authors： Lu G,Xu C,Tang K,Zhang J,Li Q,Peng L,Wang Y,Huang Z,Gao X

更新日期：2017-01-29 00:00:00

abstract：:Deficiency of Smad3, an intracellular mediator of TGF-β, was shown to significantly accelerate re-epithelialization of the colonic mucosa. This study was performed to investigate the molecular mechanisms by which Smad3 controls colonic epithelial cell proliferation and crypt formation. Smad3(ex8/ex8) C57BL/6 mice were...

journal_title：Biochemical and biophysical research communications

authors： Furukawa K,Sato T,Katsuno T,Nakagawa T,Noguchi Y,Tokumasa A,Yokote K,Yokosuka O,Saito Y

更新日期：2011-02-25 00:00:00

abstract：:Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) alpha interacting protein by yeast two-hybrid screening. I...

journal_title：Biochemical and biophysical research communications

authors： Qi C,Zhu YT,Chang J,Yeldandi AV,Rao MS,Zhu YJ

更新日期：2005-03-11 00:00:00

abstract：:Epididymal fluid from rats rendered infertile by oral administration of ornidazole contains a protein CAP1 (contraception-associated protein 1) that is absent from epididymal fluid, but present on epididymal sperm, from fertile vehicle-treated rats. The gene for CAP1 has been isolated from a rat testis cDNA library an...

journal_title：Biochemical and biophysical research communications

authors： Wagenfeld A,Gromoll J,Cooper TG

更新日期：1998-10-20 00:00:00

abstract：:Protein Z was purified from rabbit liver cytosol by affinity chromatography on oleic acid-agarose and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After removal of sodium dodecyl sulfate, the renatured protein was found to bind heme and bilirubin with a Kd of approximately 1 microM which prod...

journal_title：Biochemical and biophysical research communications

authors： Vincent SH,Holeman B,Muller-Eberhard U

更新日期：1985-10-30 00:00:00

abstract：:We have constructed two phagemid vectors containing the gene coding for human Prostate Specific Antigen (PSA) translationally fused to a pelB signal sequence and minor coat protein III of phage fd leading to phage particles that carry PSA on their tips. Phages were characterized by Western blotting and by panning, usi...

journal_title：Biochemical and biophysical research communications

authors： Eerola R,Saviranta P,Lilja H,Pettersson K,Lövgren T,Karp M

更新日期：1994-05-16 00:00:00

abstract：:We previously demonstrated several nicotinic acetylcholine receptor (nAChR) subunits and associated proteins in human sperm. Here, we identified in sperm for the first time two additional nAChR-associated molecules: (1) agrin(SN)Z(+) in human sperm localized in the posterior post-acrosomal, neck, and flagellar mid-pie...

journal_title：Biochemical and biophysical research communications

authors： Kumar P,Ferns MJ,Meizel S

更新日期：2006-04-07 00:00:00

abstract：:Tyrosine hydroxylase (TH) is the rate-limiting enzyme in catecholamine biosynthesis, and its N-terminus plays a critical role in the intracellular stability of the enzyme. In the present study, we investigated the mechanism by which the N-terminal region of TH affects this stability. TH molecules phosphorylated at the...

journal_title：Biochemical and biophysical research communications

authors： Nakashima A,Mori K,Kaneko YS,Hayashi N,Nagatsu T,Ota A

更新日期：2011-04-08 00:00:00

abstract：:Ligand-blotting and dot-blotting procedures were used to investigate the binding of [125I]-heparin to apolipoprotein E, its thrombin fragments E22 (residues 1-191) and E12 (residues 192-299), and to nine apolipoprotein E synthetic fragments. E22 and E12 bound [125I] heparin indicating multiple heparin-binding domains....

journal_title：Biochemical and biophysical research communications

authors： Cardin AD,Hirose N,Blankenship DT,Jackson RL,Harmony JA,Sparrow DA,Sparrow JT

更新日期：1986-01-29 00:00:00

abstract：:Chloride channels in the ocular ciliary epithelium are believed to play a key role in aqueous humor formation. We isolated a cDNA clone from a lambda Uni-ZAP cDNA library of human nonpigmented ciliary epithelial (NPE) cells encoding the swelling-induced chloride channel/channel regulator pICln. The human clone contain...

journal_title：Biochemical and biophysical research communications

authors： Anguita J,Chalfant ML,Civan MM,Coca-Prados M

更新日期：1995-03-08 00:00:00

abstract：:Platelet aggregation plays a major role in thrombogenesis. This study was undertaken to examine the inhibition of platelet aggregation induced by adenosine diphosphate. It is known that cyclic AMP (adenosine monophosphate) and its dibutyryl derivative inhibit platelet aggregation. This study showed that prostagland...

journal_title：Biochemical and biophysical research communications

authors： Marquis NR,Vigdahl RL,Tavormina PA

更新日期：1969-09-10 00:00:00

abstract：BACKGROUND:- Tadalafil, a long-acting phosphodiesterase 5 (PDE5) inhibitor, alleviates preeclampsia (PE), and decreases the fetal and infant deaths associated with fetal growth restriction (FGR) in phase II clinical trial. Recently, we demonstrated that tadalafil alleviates FGR and hypertension in the dams with PE indu...

journal_title：Biochemical and biophysical research communications

authors： Sekimoto A,Tanaka K,Hashizume Y,Sato E,Sato H,Ikeda T,Takahashi N

更新日期：2020-01-15 00:00:00

abstract：:Wortmannin caused normal and strong inhibition on catecholamine secretion from bovine adrenal chromaffin cells and in vitro phosphoinositide 3-kinase activity in NaCl-, Na isethionate-, choline Cl-, Na acetate-, and N-acetyl glycine-based media. However, brief preincubation of wortmannin with the media containing amin...

journal_title：Biochemical and biophysical research communications

authors： Isosaki M

更新日期：2004-11-26 00:00:00

abstract：:The transcription regulator SNAI1 triggers a transcriptional program leading to epithelial to mesenchymal transition (EMT), providing epithelial cells with mesenchymal features and invasive properties during embryonic development and tumor progression. To identify early transcriptional changes occurring during SNAI1-i...

journal_title：Biochemical and biophysical research communications

authors： Vetter G,Le Béchec A,Muller J,Muller A,Moes M,Yatskou M,Al Tanoury Z,Poch O,Vallar L,Friederich E

更新日期：2009-08-07 00:00:00