Expression of peroxisome proliferator-activated receptor gamma (PPARgamma) in rat aortic smooth muscle cells.

abstract：:A general strategy is described for using the cleavage site of restriction enzymes in vectors for cloning regardless of how many sites the given enzymes have in the vector. The application of this method allows one to open any vector at its cloning site with protruding ends which can be compatible with almost every co...

journal_title：Biochemical and biophysical research communications

authors： Welker E,Váradi A

更新日期：2000-05-10 00:00:00

abstract：:Peroxynitrite, formed by the interaction of superoxide with nitric oxide, has previously been implicated mostly as a cytotoxic agent. In contrast, its physiological and, possibly, beneficial effects are largely unknown. We have previously shown [Journal of Biological Chemistry, 1997, 272, 7253] that RAW 264.7 macropha...

journal_title：Biochemical and biophysical research communications

authors： Scivittaro V,Boggs S,Mohr S,Lapetina EG

更新日期：1997-12-08 00:00:00

abstract：:The plasma half-lives of glycosylated and unglycosylated alpha 1-proteinase inhibitor-radioactively labeled with [35S]methionine in rat hepatocyte primary cultures - were determined in the rat. Unglycosylated alpha 1-proteinase inhibitor was synthesized by hepatocytes in the presence of tunicamycin. Media from hepatoc...

journal_title：Biochemical and biophysical research communications

authors： Weber W,Steube K,Gross V,Tran-Thi TA,Decker K,Gerok W,Heinrich PC

更新日期：1985-01-16 00:00:00

abstract：:We present the first direct biochemical evidence for the turnover of intact type VI collagen microfibrils. Matrix-degrading enzymes of the serine proteinase class, including rat mast cell chymases I and II, human mast cell tryptase, neutrophil elastase, cathepsin G and trypsin, were able to catabolize intact type VI c...

journal_title：Biochemical and biophysical research communications

authors： Kielty CM,Lees M,Shuttleworth CA,Woolley D

更新日期：1993-03-31 00:00:00

abstract：:Prior to the somite segmentation, the cells in the anterior presomitic mesoderm (PSM) express a set of genes that is required for defining the segmental border and polarity of the prospective somite. However, little is known how the expression of these genes is repressed upon segmentation. Here we report that Bowline,...

journal_title：Biochemical and biophysical research communications

authors： Kondow A,Hitachi K,Okabayashi K,Hayashi N,Asashima M

更新日期：2007-08-10 00:00:00

abstract：:Hydrogen sulfide (H(2)S) is an important gasotransmitter that generated in mammalian cells from l-cysteine metabolism. Little is known about its protective role in oxidative stress. In the present study, we investigated whether H(2)S could affect homocysteine (HCY)-induced cytotoxicity and oxidative stress in vascular...

journal_title：Biochemical and biophysical research communications

authors： Yan SK,Chang T,Wang H,Wu L,Wang R,Meng QH

更新日期：2006-12-15 00:00:00

abstract：:Cachexia, observed in most cancer patients, is a syndrome that includes wasting of bodily energy reserves and is characterized by muscle atrophy and fat loss. We have previously demonstrated that isoflavones, such as genistein and daidzein, prevent muscle wasting in tumor-bearing mice. In this study, we examined the e...

journal_title：Biochemical and biophysical research communications

authors： Yoshimura T,Saitoh K,Sun L,Wang Y,Taniyama S,Yamaguchi K,Uchida T,Ohkubo T,Higashitani A,Nikawa T,Tachibana K,Hirasaka K

更新日期：2018-12-02 00:00:00

abstract：:The mutation responsible for the juvenile form of Batten disease was mapped to a single gene, Cln3 (T. J. Lerner et al. (1995) Cell 82:949-957). Yeast Saccharomyces cerevisiae has an open reading frame, BTN1 (YHC3), that encodes the putative homologue of Cln3p. Primary structure comparison indicates that the human Cln...

journal_title：Biochemical and biophysical research communications

authors： Croopnick JB,Choi HC,Mueller DM

更新日期：1998-09-18 00:00:00

abstract：:In platelets, activation of protein kinase C and mobilization of Ca2+ were selectively induced by the addition of 1-oleoyl-2-acetyl-glycerol and a low concentration of A23187, respectively (Kaibuchi, K., Takai, Y., Sawamura, M., Hoshijima, M., Fujikura, T. and Nishizuka, Y. (1983) J. Biol. Chem. 258, 6701-6704). Using...

journal_title：Biochemical and biophysical research communications

authors： Kajikawa N,Kaibuchi K,Matsubara T,Kikkawa U,Takai Y,Nishizuka Y,Itoh K,Tomioka C

更新日期：1983-10-31 00:00:00

abstract：:Recently, a novel oxysterol, 5-cholesten-3beta, 25-diol 3-sulfate (25HC3S) was identified in primary rat hepatocytes following overexpression of the cholesterol transport protein, StarD1. This oxysterol was also detected in human liver nuclei. In the present study, 25HC3S was chemically synthesized. Addition of 25HC3S...

journal_title：Biochemical and biophysical research communications

authors： Ren S,Li X,Rodriguez-Agudo D,Gil G,Hylemon P,Pandak WM

更新日期：2007-09-07 00:00:00

abstract：:Reversible phosphorylation of RNA polymerase (RNAP) II's largest subunit C-terminal domain (CTD) is a key event during mRNA metabolism. The CTD phosphatase, FCP1, catalyzes the dephosphorylation of RNAP II and is thought to play a major role in polymerase recycling. In this study, we isolated a novel phosphatase gene ...

journal_title：Biochemical and biophysical research communications

authors： Zheng H,Ji C,Gu S,Shi B,Wang J,Xie Y,Mao Y

更新日期：2005-06-17 00:00:00

abstract：:A growing body of evidence suggests that MYC induced long noncoding RNA (MINCR) is involved in the initiation and progression of various tumors. However, little is known about the biological function and clinical value of MINCR in non-small cell lung cancer (NSCLC). In the present study, results found that MINCR over ...

journal_title：Biochemical and biophysical research communications

authors： Wang J,Ding M,Zhu H,Cao Y,Zhao W

更新日期：2019-01-15 00:00:00

abstract：:ATP binding cassette (ABC) transporters have a functional unit formed by two transmembrane domains and two nucleotide binding domains (NBDs). ATP-bound NBDs dimerize in a head-to-tail arrangement, with two nucleotides sandwiched at the dimer interface. Both NBDs contribute residues to each of the two nucleotide-bindin...

journal_title：Biochemical and biophysical research communications

authors： Zoghbi ME,Altenberg GA

更新日期：2014-01-03 00:00:00

abstract：:In chaperoning dithiothreitol-denatured alpha-lactabumin, alpha-crystallin forms a chaperoning complex. In order to study the kinetics of such chaperoning it needs to be established whether the formation of the chaperoning complex is a reversible or irreversible process. The chaperoning reaction was studied by dynamic...

journal_title：Biochemical and biophysical research communications

authors： Neal R,Zigler JS Jr,Bettelheim FA

更新日期：2001-01-12 00:00:00

abstract：:Usefulness of adenoviral vectors derived from human adenovirus (HAd) type 5 (HAd5) is mainly limited by wide prevalence of preexisting anti-HAd5 immunity as well as non-specific tissue tropism of these vectors. As an alternative, non-human adenoviral vectors including bovine adenovirus type 3 (BAd3) are currently bein...

journal_title：Biochemical and biophysical research communications

authors： Bangari DS,Sharma A,Mittal SK

更新日期：2005-06-17 00:00:00

abstract：:Recently it has been shown that vascular endothelial cells (EC) are completely devoid of apoptosis if cultivated under a steady laminar flow and that apoptosis is induced by turning off the flow. An autocrine loop of thrombospondin-1 (TSP-1) and the alpha(v)beta(3) integrin/integrin-associated protein (IAP) complex ha...

journal_title：Biochemical and biophysical research communications

authors： Freyberg MA,Kaiser D,Graf R,Buttenbender J,Friedl P

更新日期：2001-08-10 00:00:00

abstract：:Nutritional and pharmacological factors are needed to prevent bone loss that occurs with increasing age. The chemical compounds that act on bone metabolism as nutrients in food, however, are poorly understood. The effect of resveratrol, a natural phytoestrogen, on the proliferation and differentiation of osteoblastic ...

journal_title：Biochemical and biophysical research communications

authors： Mizutani K,Ikeda K,Kawai Y,Yamori Y

更新日期：1998-12-30 00:00:00

abstract：:Exposure of [3H]-lysine labeled elastin to either purified myeloperoxidase plus H2O2 and halides or human neutrophils plus phorbol myristate acetate resulted in oxidation of lysine side chains quantitated as 3H2O release. In both the enzyme and cell system oxidation was blocked by azide, cyanide or catalase, but not b...

journal_title：Biochemical and biophysical research communications

authors： Clark RA,Szot S,Williams MA,Kagan HM

更新日期：1986-03-13 00:00:00

abstract：:The patch clamp technique applied to canine atrial myocyte sarcolemma, showed that 2 types of K+ channels were differentially affected by Ach. The 2 channel types differed in conductance, responses to [Ca++], and sensitivity to membrane potential. Addition of Ach (10(-9) M to 10(-6) M) to pipette solutions increased o...

journal_title：Biochemical and biophysical research communications

authors： Bubien JK,Woods WT Jr

更新日期：1987-02-13 00:00:00

abstract：:Drosophila Dicer-2 processes RNA substrates into short interfering RNAs (siRNAs). Loquacious-PD (Loqs-PD), a dsRNA-binding protein that associates with Dicer-2, is required for processing of a subset of RNA substrates including hairpin RNAs into siRNAs. Inorganic phosphate-a small molecule present in all cell types-in...

journal_title：Biochemical and biophysical research communications

authors： Fukunaga R

更新日期：2018-04-15 00:00:00

abstract：:Mammalian homologues of the Drosophila trp gene have been invoked as the structural basis for the currents associated with capacitative Ca2+ entry (CCE) in many cell types. Trp homologues are members of a large protein family that may associate as channel subunits providing an explanation for the functional diversity ...

journal_title：Biochemical and biophysical research communications

authors： Garcia RL,Schilling WP

更新日期：1997-10-09 00:00:00

abstract：:This study examines the influence of insoluble matrix components of glioma (astrocytoma) cells on LPS-mediated inducible nitric oxide (NO)/NO synthase (iNOS) induction in microglia cells. Insoluble matrix components prepared from C6 rat glioma cells strongly suppressed iNOS induction and subsequent NO release induced ...

journal_title：Biochemical and biophysical research communications

authors： Kim YJ,Hwang SY,Han IO

更新日期：2006-09-01 00:00:00

abstract：:The Mrr protein of Escherichia coli K12 is a cryptic Type IV restriction endonuclease whose activity appears to be triggered by high pressure stress. In this report we used high pressure to isolate and analyze several Mrr mutants, and generated a new structural model of the Mrr protein. The activity of a number of spo...

journal_title：Biochemical and biophysical research communications

authors： Orlowski J,Mebrhatu MT,Michiels CW,Bujnicki JM,Aertsen A

更新日期：2008-12-19 00:00:00

abstract：:C1 inhibitor (C1IN) is a multi-facet serine protease inhibitor in the plasma cascades, inhibiting several proteases, notably, regulates both complement and contact system activation. Despite huge advancements in the understanding of C1IN based on biochemical properties and its roles in the plasma cascades, the phyloge...

journal_title：Biochemical and biophysical research communications

authors： Kumar A,Bhandari A,Sarde SJ,Goswami C

更新日期：2014-07-18 00:00:00

abstract：:Periplasmic proteins of Gram-negative bacteria like Escherichia coli are subjected to immediate affect of environmental fluctuation that may unfold proteins, due to the permeability of the outer membrane to small molecules. They are thus supposedly protected by certain molecular chaperones. Nevertheless, no homologues...

journal_title：Biochemical and biophysical research communications

authors： Liu Y,Fu X,Shen J,Zhang H,Hong W,Chang Z

更新日期：2004-04-09 00:00:00

abstract：:Increased microvessel density in atherosclerotic plaques plays a major role in promoting plaque destabilization resulting in increased risk of stroke and myocardial infarction. Previously we have shown that expression of the inflammatory cytokine, Oncostatin-M (OSM), in human atherosclerotic plaques correlated with in...

journal_title：Biochemical and biophysical research communications

authors： Albasanz-Puig A,Murray J,Namekata M,Wijelath ES

更新日期：2012-11-09 00:00:00

abstract：:The yellow locus in Drosophila is involved in both cuticle development and behaviour. However, the function of the encoded protein is unknown. Here we have characterised the sequence and expression pattern of a new Drosophila gene, designated yellow-B, encoding a 453-amino-acid protein that is 57% identical to Yellow....

journal_title：Biochemical and biophysical research communications

authors： Maleszka R,Kucharski R

更新日期：2000-04-21 00:00:00

abstract：:A decline in intracellular nicotinamide adenine mononucleotide (NAD+) causes adipose tissue dysfunction. Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the rate-limiting step in the NAD+ biosynthesis pathway. However, the molecular mechanism that mediates regulation of NAMPT expression in adipocytes is yet t...

journal_title：Biochemical and biophysical research communications

authors： Mitani T,Watanabe S,Wada K,Fujii H,Nakamura S,Katayama S

更新日期：2020-02-12 00:00:00

abstract：:In general, viral infection is supposed to induce stress responses in the host cell. However, very few detailed observations about virus-induced stress responses have been reported. Here we investigated specific stress responses in Vero cells infected with Sindbis virus (SV), a single-stranded RNA virus, acute infecti...

journal_title：Biochemical and biophysical research communications

authors： Nakatsue T,Katoh I,Nakamura S,Takahashi Y,Ikawa Y,Yoshinaka Y

更新日期：1998-12-09 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) is a gamma-herpesvirus that is frequently associated with the formation of tumors like Kaposi's sarcoma and primary effusion lymphomas. KSHV RTA is required for lytic replication/reactivation in KSHV-infected cells. We developed an inducible RTA expression cell line to se...

journal_title：Biochemical and biophysical research communications

authors： Yada K,Do E,Sakakibara S,Ohsaki E,Ito E,Watanabe S,Ueda K

更新日期：2006-06-23 00:00:00