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Stimulation of Mn-superoxide dismutase expression by tumor necrosis factor-alpha: quantitative determination of Mn-SOD protein levels in TNF-resistant and sensitive cells by ELISA.

Abstract：

:Marked increase in protein levels of Mn-superoxide dismutase (Mn-SOD) was found in TNF-resistant cell lines after treatment with Tumor Necrosis Factor (TNF). No such increase was observed in Cu,Zn-superoxide dismutase (Cu, Zn-SOD) protein in either TNF-resistant or sensitive cells. These results support the data that the Mn-SOD is one of the rescue proteins required for resistance to TNF cytotoxicity in these cell lines (Wong et al., Cell 58, 923-931, 1990). Mn-SOD was also responsive to TNF stimulation in KURAMOCHI, a human ovarian adenocarcinoma cell line. This may explain our previous result that Mn-SOD protein is highly expressed in epithelial ovarian cancer (Ishikawa et al. Cancer Res. 50, 2538-2542, 1990).

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kawaguchi T,Takeyasu A,Matsunobu K,Uda T,Ishizawa M,Suzuki K,Nishiura T,Ishikawa M,Taniguchi N

doi

10.1016/0006-291x(90)90838-e

subject

Has Abstract

pub_date

1990-09-28 00:00:00

pages

1378-86

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(90)90838-E

journal_volume

171

pub_type

杂志文章

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