Solution structure of intracellular signal-transducing peptide derived from human beta(2)-adrenergic receptor.

abstract：:Activation of the classical IκB kinases (IKKα and IKKβ) was previously shown to contribute to obesity-induced inflammation and insulin resistance. Using knockout mice, we investigated whether the related isoform IKKε plays a similar metabolic role. IKKε(-/-) mice had reduced body weight, leptin levels, as well as high...

journal_title：Biochemical and biophysical research communications

authors： Scheja L,Heese B,Seedorf K

更新日期：2011-04-08 00:00:00

abstract：:Ubiquitylation is an essential posttranslational protein modification that is implicated in a diverse array of cellular functions. Although cells contain eight structurally distinct types of polyubiquitin chains, detailed function of several chain types including K29-linked chains has remained largely unclear. Current...

journal_title：Biochemical and biophysical research communications

authors： Tsuchiya H,Tanaka K,Saeki Y

更新日期：2013-06-28 00:00:00

abstract：:A P1 phage clone containing the near complete protein coding region of the human histo-blood group ABO gene locus was isolated and characterized. The insert was estimated at approximately 41 kp, and an approximately 20 kbp BamHI fragment contained the near complete coding region as evaluated by Southern blot analysis....

journal_title：Biochemical and biophysical research communications

authors： Bennett EP,Steffensen R,Clausen H,Weghuis DO,van Kessel AG

更新日期：1995-01-05 00:00:00

abstract：:Genomic DNA of the phytopathogenic Erwinia carotovora subsp. carotovora LY34 was partially digested with Sau3AI, ligated into the BamHI site of pBlue-script II SK+, and introduced into E. coli. Two clones that were able to hydrolyse carboxymethylcellulose were selected. 1.5 kb and 1.2 kb fragments containing the celA ...

journal_title：Biochemical and biophysical research communications

authors： Park YW,Lim ST,Cho SJ,Yun HD

更新日期：1997-12-29 00:00:00

abstract：:ABCG2 (also called MXR (3), BCRP (4), or ABCP (5) is a recently-identified ABC half-transporter, which causes multidrug resistance in cancer. Here we report that the expression of the ABCG2 protein in Sf9 insect cells resulted in a high-capacity, vanadate-sensitive ATPase activity in isolated membrane preparations. AB...

journal_title：Biochemical and biophysical research communications

authors： Ozvegy C,Litman T,Szakács G,Nagy Z,Bates S,Váradi A,Sarkadi B

更新日期：2001-07-06 00:00:00

abstract：:Signaling by TNF-family receptor CD40 involves TRAF-family adaptor proteins, leading to activation of protein kinases that induce NFkappaB-family transcription factors. We report here that mitogen activated protein kinase kinase kinase-8 (MAP3K8), Tpl2/COT1, is recruited to the CD40 complex via a mechanism dependent o...

journal_title：Biochemical and biophysical research communications

authors： Chan H,Reed JC

更新日期：2005-03-04 00:00:00

abstract：:An aminopeptidase secreted from Streptomyces septatus TH-2 (SSAP) was identified as a heat stable enzyme, and the Ssap gene was cloned and sequenced. The primary structure of SSAP showed 71% identity with that of a Streptomyces griseus aminopeptidase (SGAP), however, it lacked a unique calcium binding site. The recomb...

journal_title：Biochemical and biophysical research communications

authors： Arima J,Iwabuchi M,Hatanaka T

更新日期：2004-04-30 00:00:00

abstract：:Tetrahymena p85 is localized to the presumptive division plane before division furrow formation; its molecular weight in SDS-polyacrylamide gel electrophoresis differs in wild-type and temperature-sensitive cell-division-arrest mutant cdaA1 cells. At the restrictive temperature, p85 localization and division furrow fo...

journal_title：Biochemical and biophysical research communications

authors： Gonda K,Nishibori K,Ohba H,Watanabe A,Numata O

更新日期：1999-10-14 00:00:00

abstract：:Endemic Alkanna cappadocica was used to isolate novel antitumor molecules from Turkish landscapes in our previous studies. In this study, deoxyalkannin (ALCAP1), β,β-dimethylacrylalkannin (ALCAP2), acetylalkannin (ALCAP3), and alkannin (ALCAP4) as well as the novel isolated compounds 5-methoxydeoxyalkannin (ALCAP5), 8...

journal_title：Biochemical and biophysical research communications

authors： Bogurcu N,Sevimli-Gur C,Ozmen B,Bedir E,Korkmaz KS

更新日期：2011-06-17 00:00:00

abstract：:We have investigated the subcellular localization of the inwardly rectifying K+ channel subunit Kir6.1 (uKATP-1). Immunoblot analysis of the mitochondrial fractions prepared from rat skeletal muscle and liver detected a single band of Kir6.1 at 51 kDa, the intensity of which was stronger than that found in the total h...

journal_title：Biochemical and biophysical research communications

authors： Suzuki M,Kotake K,Fujikura K,Inagaki N,Suzuki T,Gonoi T,Seino S,Takata K

更新日期：1997-12-29 00:00:00

abstract：:Insulin-like growth factor I stimulates mitogenesis in smooth muscle cells, and upregulates elastin synthesis in embryonic aortic tissue. Increased smooth muscle elastin synthesis may play an important role in vascular remodeling in chronic pulmonary hypertension. Therefore, we studied the effect of IGF-I on elastin a...

journal_title：Biochemical and biophysical research communications

authors： Badesch DB,Lee PD,Parks WC,Stenmark KR

更新日期：1989-04-14 00:00:00

abstract：:In a chicken B cell line, DT40, the disruption of HDAC2 (chHDAC2) gene causes an alteration of several gene expressions including chicken IgM light chain (chIgM-L) gene by 2D-PAGE analysis. To investigate the transcriptional function of chHDAC2, we employed the chIgM-L promoter reporter plasmid. We found that chHDAC2 ...

journal_title：Biochemical and biophysical research communications

authors： Takechi S,Adachi M,Nakayama T

更新日期：2002-11-29 00:00:00

abstract：:Collagens are the most abundant structural proteins found in the extracellular matrix of vertebrates. Knowledge of the mechanical behavior of collagen monomers is essential for understanding the mechanical properties of collagen fibrils that constitute the main architectural framework of skin, bone, cartilage, and oth...

journal_title：Biochemical and biophysical research communications

authors： Sun YL,Luo ZP,Fertala A,An KN

更新日期：2002-07-12 00:00:00

abstract：:Both the epidermal growth factor receptor (EGFR) and type 1 insulin-like growth factor receptor (IGF1R) require homo- and hetero-dimerisation with their own family members to acquire full function. We recently showed that IGF1R gene silencing led to EGFR hyper-phosphorylation in human breast cancer cells, and hypothes...

journal_title：Biochemical and biophysical research communications

authors： Riedemann J,Takiguchi M,Sohail M,Macaulay VM

更新日期：2007-04-13 00:00:00

abstract：:Two different clones, named rEP3A (approximately 2.2 kb) and rEP3B (approximately 5.2 kb), were isolated from a rat kidney cDNA library by a homology screening approach. rEP3A was shown to encode the rat kidney prostaglandin E receptor EP3 subtype (rEP3A receptor) (Takeuchi, K. et al. B.B.R.C. (1993) 194: 885). rEP3B ...

journal_title：Biochemical and biophysical research communications

authors： Takeuchi K,Takahashi N,Abe T,Abe K

更新日期：1994-03-15 00:00:00

abstract：:Insulin-stimulated GLUT4 recruitment to the plasma membrane is impaired in insulin resistance. We recently reported that a cell permeable phosphoinositide-binding peptide induces GLUT4 recruitment as potently as insulin, but does not activate GLUT4 to initiate glucose uptake. Here we investigated whether the peptide-i...

journal_title：Biochemical and biophysical research communications

authors： Funaki M,Benincasa K,Randhawa PK

更新日期：2007-09-07 00:00:00

abstract：:A novel iron-binding protein from rat liver homogenates was purified 1,800-fold with a 5.7% yield, to apparent homogeneity. The molecular weight of the protein was estimated to be 16,000, by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The purified protein exhibited 0.43 mol of iron bi...

journal_title：Biochemical and biophysical research communications

authors： Furukawa T,Taketani S,Kohno H,Tokunaga R

更新日期：1991-11-27 00:00:00

abstract：:The effects of selective inhibition of cyclic AMP phosphodiesterase type III on insulin and glucose levels during an oral glucose challenge were evaluated in obese, diabetic ob/ob mice and in lean, non-diabetic littermates using the selective inhibitor, milrinone. Oral administration of milrinone increased plasma insu...

journal_title：Biochemical and biophysical research communications

authors： Parker JC,VanVolkenburg MA,Nardone NA,Hargrove DM,Andrews KM

更新日期：1997-07-30 00:00:00

abstract：:Human immunodeficiency virus type-1 (HIV-1) envelope glycoprotein gp140 interacts with its specific receptors on the surface of the target cells leading to cellular activation through various signaling pathways. The effect of blocking the chemokine repertoire in human brain microvascular endothelial cells in HIV demen...

journal_title：Biochemical and biophysical research communications

authors： Mendu DR,Katinger H,Sodroski J,Kim KS

更新日期：2007-11-23 00:00:00

abstract：:MelC1 regulates the copper incorporation and secretion of Streptomyces apotyrosinase (MelC2) via a transient, competent complex formation. His-102 and His-117 of the chaperone-like MelC1 are known to play important roles in this trans-activation activity of MelC1. In this study, we studied the side chain requirement a...

journal_title：Biochemical and biophysical research communications

authors： Liaw LL,Lee YH

更新日期：1995-09-14 00:00:00

abstract：:Chitin oligosaccharides elicited chitinase activity in melon plants. Hexamer to nonamer were the most efficient elicitors: hexamer for maximal stimulation of colorimetrically assessed chitinase activity, heptamer for maximal stimulation of radiochemically assessed chitinase activity. Chitinase elicitation was a rapid ...

journal_title：Biochemical and biophysical research communications

authors： Roby D,Gadelle A,Toppan A

更新日期：1987-03-30 00:00:00

abstract：:Inhibition experiments with D-mannose oligosaccharides establish that conglutinin recognises terminal alpha 1----2 mannobiosyl units present in the glycopeptide of the alpha-chain of the complement component C3b. On the basis of its three domain structure and the homology of its N-terminal amino-acid sequence to that ...

journal_title：Biochemical and biophysical research communications

authors： Young NM,Leon MA

更新日期：1987-03-13 00:00:00

abstract：:2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and related compounds modulate various endocrine functions by enhancing ligand metabolism, altering hormone synthesis, down regulating receptor levels, and interfering with gene transcription. In the present study, we investigated the effects of TCDD on testosterone signal tr...

journal_title：Biochemical and biophysical research communications

authors： Jana NR,Sarkar S,Ishizuka M,Yonemoto J,Tohyama C,Sone H

更新日期：1999-03-24 00:00:00

abstract：:Protection against hypoxic injury by supraphysiological glycine and alanine concentrations was investigated in the isolated perfused rat kidney (IPRK). 23Na NMR detects consistent increases in total renal Na in IPRK during hypoxic perfusion. Increasing the concentration of glycine and alanine to 5 mM each produced a 3...

journal_title：Biochemical and biophysical research communications

authors： Endre ZH,Cowin GJ,Stewart-Richardson P,Cross M,Willgoss DA,Duggleby RG

更新日期：1994-08-15 00:00:00

abstract：:Four monoclonal antibodies, M-T21, M-T32, M-T41 and UM4D4, which belong to the new CDw 60 cluster of antibodies specific for a subpopulation of human T-lymphocytes, were found to bind mainly to acetylated forms of ganglioside GD3. After O-deacetylation of the antigen, binding was reduced ("M-T"-antibodies) or abolishe...

journal_title：Biochemical and biophysical research communications

authors： Kniep B,Peter-Katalinić J,Flegel W,Northoff H,Rieber EP

更新日期：1992-09-30 00:00:00

abstract：:The author found a sugar-containing 70 KDa factor in rat liver nuclei as described in the previous report (1), which repressed RNA chain initiation by all classes of rat liver RNA polymerases and specifically stimulated chromatin-dependent RNA chain elongation catalyzed by RNA polymerase II. In this communication, the...

journal_title：Biochemical and biophysical research communications

authors： Okai Y

更新日期：1984-11-30 00:00:00

abstract：:To investigate regulation mechanisms of G2/M phase transition, we studied the association of cell cycle progression with p53-dependent p21/waf-1 and cyclinG expression. We used doxorubicin (DOX) and sodium butyrate (NaB) to accumulate p53 protein. DOX treatment resulted in an apparent increase of cells in the G2/M fra...

journal_title：Biochemical and biophysical research communications

authors： Shimizu A,Nishida J,Ueoka Y,Kato K,Hachiya T,Kuriaki Y,Wake N

更新日期：1998-01-26 00:00:00

abstract：:The GABAA receptor (GABAAR) is the main inhibitory receptor in the adult mammalian brain. GABAAR function is dependent on its expression, distribution, and the chloride (Cl-) transmembrane gradient, which is determined by the potassium-chloride cotransporter 2 (KCC2) in the adult brain. KCC2 and GABAAR are downregulat...

journal_title：Biochemical and biophysical research communications

authors： Wan L,Chen L,Yu J,Wang G,Wu Z,Qian B,Liu X,Wang Y

更新日期：2020-11-12 00:00:00

abstract：:The bromodomain and extraterminal (BET) domain family proteins are epigenetic modulators involved in the reading of acetylated lysine residues. The first BET protein inhibitor to be identified, (+)-JQ1, a thienotriazolo-1, 4-diazapine, binds selectively to the acetyl lysine-binding pocket of BET proteins. We evaluated...

journal_title：Biochemical and biophysical research communications

authors： Goupille O,Penglong T,Kadri Z,Granger-Locatelli M,Fucharoen S,Maouche-Chrétien L,Prost S,Leboulch P,Chrétien S

更新日期：2016-04-15 00:00:00

abstract：:Down regulation of prolactin (PRL) receptors resulting from i.v. infusion of oPRL or human growth hormone (hGH) into female virgin rats was demonstrated. A decrease of over 85% in the number of free receptors was achieved within 15 - 30 min using infusion of oPRL or hGH at 25 micrograms/h and remained at this level un...

journal_title：Biochemical and biophysical research communications

authors： Barash I,Madar Z,Gertler A

更新日期：1983-10-31 00:00:00