Differential roles of two consecutive phenylalanine residues in thrombin receptor-tethered ligand peptides (SFFLRNP) in thrombin receptor activation.

abstract：:Recent data has suggested a role for nitric oxide (NO) both in the induction of immunity and as an effector of tissue injury in experimental models of inflammation. In this study, we have tested the efficacy of two inhibitors of NO synthase, NG-monomethyl-L-arginine (L-NMMA) and aminoguanidine (AG), to modify the auto...

journal_title：Biochemical and biophysical research communications

authors： Woolfson RG,Qasim FJ,Thiru S,Oliveira DB,Neild GH,Mathieson PW

更新日期：1995-12-14 00:00:00

abstract：:PhyA gene products of Aspergillus ficuum (AF) and Peniophora lycii (PL) as expressed in industrial strains of Aspergillus niger and Aspergillus oryzae, respectively, were purified to homogeneity and then characterized for both physical and biochemical properties. The PL phytase is 26 amino acid residues shorter than t...

journal_title：Biochemical and biophysical research communications

authors： Ullah AH,Sethumadhavan K

更新日期：2003-04-04 00:00:00

abstract：:To study the effect of interleukin-6 (IL-6) on glucose transport, 2-deoxy-D-glucose (2-DOG) uptake in 3T3-L1 adipocytes after incubation with IL-6 was measured. IL-6 increased 2-DOG uptake maximally after 5 hours by 30 +/- 12%, with a half-maximal effect at an IL-6 concentration of approximately 800 U/mL. 3-O-methylgl...

journal_title：Biochemical and biophysical research communications

authors： Stouthard JM,Oude Elferink RP,Sauerwein HP

更新日期：1996-03-18 00:00:00

abstract：:We found that heparin at low concentrations increases the vascular endothelial growth factor (VEGF)-induced proliferation of human umbilical vein endothelial cells (HUVECs) but at high concentrations decreases it. To examine whether FLT-1, a VEGF receptor, interacts with heparin and which domain of FLT-1 binds to hepa...

journal_title：Biochemical and biophysical research communications

authors： Park M,Lee ST

更新日期：1999-11-02 00:00:00

abstract：:Kainate receptors are a class of ionotropic glutamate receptors that are widely expressed in the mammalian brain, yet little is known about their physiological role or the mechanisms by which they are regulated. Kainate receptors are composed of multiple subunits (GluR5-7; KA1-2), which can combine to form homomeric o...

journal_title：Biochemical and biophysical research communications

authors： Hayes DM,Braud S,Hurtado DE,McCallum J,Standley S,Isaac JT,Roche KW

更新日期：2003-10-10 00:00:00

abstract：:Polyphosphoinositide hydrolysis was examined in membranes from thyrotropin-releasing hormone (TRH)-responsive GH3 pituitary cells. [3H]Inositol phosphates (IP2 and IP3) were generated upon incubation of membranes from [3H]inositol-labeled cells indicating the presence of a membrane-associated polyphosphoinositide phos...

journal_title：Biochemical and biophysical research communications

authors： Lucas DO,Bajjalieh SM,Kowalchyk JA,Martin TF

更新日期：1985-10-30 00:00:00

abstract：:A truncated form of 24kDa FGF-2 consisting of 86 NH(2)-terminal amino acids (ATE+31) inhibits cell migration in vitro and tumor development and angiogenesis in vivo. Focal adhesion kinase (FAK) is phosphorylated on tyrosine and serine sites after cell stimulation by growth factors. This study examined the effect of AT...

journal_title：Biochemical and biophysical research communications

authors： Lin AH,Eliceiri BP,Levin EG

更新日期：2009-05-08 00:00:00

abstract：:An exposure of a human myeloma cell line to 2-difluoromethylornithine the mechanism-based inhibitor of ornithine decarboxylase (EC 4.1.1.17), resulted in a selection of tumor cells readily growing in the presence of 4 mM difluoromethylornithine, a concentration that swiftly halted the growth of the parental cells. Det...

journal_title：Biochemical and biophysical research communications

authors： Alhonen-Hongisto L,Leinonen P,Laine R,Jänne J

更新日期：1987-04-14 00:00:00

abstract：:The aim of this study is to investigate the mechanism underling cardiac dysfunction during sepsis, as well as the possible amelioration of this dysfunction by exogenous carbon monoxide (CO) administration. For this purpose, rats (six-week-old, male, Sprague-Dawley) were administered LPS (15 mg/kg body weight, i.p. 6 h...

journal_title：Biochemical and biophysical research communications

authors： Unuma K,Aki T,Nagano S,Watanabe R,Uemura K

更新日期：2018-01-08 00:00:00

abstract：:Previous studies have shown that some inflammatory cytokines promote the expression of corticotropin-releasing hormone (CRH) in trophoblasts during pregnancy and that placental CRH could induce the production of adrenocorticotropic hormone (ACTH) in humans. However, whether the same is true in rodent placenta remains ...

journal_title：Biochemical and biophysical research communications

authors： Wang H,Tsukada T,Shimada H,Sakata-Haga H,Iida Y,Zhang S,Shoji H,Hatta T

更新日期：2020-01-29 00:00:00

abstract：:A large number of mutations in rhodopsin are associated with autosomal dominant retinitis pigmentosa (ADRP). We analyzed the biochemical phenotypes of the ADRP-associated cysteine mutants C167R, C222R, and C264del. C222R behaved as wild type in every aspect testable and is classified as a class I mutant. C167R produce...

journal_title：Biochemical and biophysical research communications

authors： Breikers G,Portier-VandeLuytgaarden MJ,Bovee-Geurts PH,DeGrip WJ

更新日期：2002-10-04 00:00:00

abstract：:Myocardial cells respond to changes in the mechanical forces imposed on them with changes in myocardial tension in the short term and with structural remodeling in the long term. Since these responses involve intercellular communication, we have investigated regulation of the gap junction proteins, connexin 43 (Cx43),...

journal_title：Biochemical and biophysical research communications

authors： Wang TL,Tseng YZ,Chang H

更新日期：2000-01-19 00:00:00

abstract：:Reversion of adenine auxotrophic strains of D. radiodurans by cross-links in DNA was studied. Mutation was induced in a wild-type but not in a mitomycin C sensitive mutant after irradiation with near UV (approximately 365nm) in the presence of 4,5',8-trimethylpsoralen (TMP). To get information on implication of cross-...

journal_title：Biochemical and biophysical research communications

authors： Yatagai F,Kitayama S

更新日期：1983-04-29 00:00:00

abstract：:Serine416 of human tau protein is believed to be phosphorylated in Alzheimer neurofibrillary tangles. We synthesized a fragment of tau, consisting of amino acids 408-421 in both non-phosphorylated and serine416-phosphorylated forms. Circular dichroism in a trifluoroethanol-water mixture indicated a beta-turn----beta-p...

journal_title：Biochemical and biophysical research communications

authors： Lang E,Szendrei GI,Elekes I,Lee VM,Otvos L Jr

更新日期：1992-01-15 00:00:00

abstract：:Cisplatin plays a key role in treating small cell lung cancer (SCLC); however, the rapid development of cisplatin resistance limits its treatment effect. The detailed mechanisms of cisplatin-resistance, particularly in SCLC, remain unclear. We analyzed the differentially expressed genes (DEGs) between cisplatin-resist...

journal_title：Biochemical and biophysical research communications

authors： Ma K,Li S,Huo X,Guo M,Du X,Li C,Liu X,Lv J,Chen Z

更新日期：2020-12-10 00:00:00

abstract：UNLABELLED:The L-arginine-nitric oxide (NO) pathway was investigated in human internal mammary artery (HIMA) in vitro. HIMA rings were mounted in organ bath, and then incubated in Krebs buffer for 1 to 8 hrs, relaxing agents were tested. Under these conditions, L-arginine (0.1 microM - 1 mM) elicited only minor relaxat...

journal_title：Biochemical and biophysical research communications

authors： Qi X,Chen D,Nottin R,Mace L,Herve P,Weiss M

更新日期：1993-08-31 00:00:00

abstract：:A series of polyamine analogs has been examined for their ability to support protein synthesis in an in vitro rabbit reticulocyte translation system. Diamines were found to stimulate protein synthesis to the greatest extent (8-12 fold). Triamines, tetraamines and pentaamines only stimulated 2-4 fold under these condit...

journal_title：Biochemical and biophysical research communications

authors： Snyder RD,Edwards ML

更新日期：1991-05-15 00:00:00

abstract：:It has been proposed that the inositol 1,4,5-trisphosphate receptor (InsP(3)R) type III acts as a trigger for InsP(3)-mediated calcium (Ca(2+)) signaling, because this InsP(3) isoform lacks feedback inhibition by cytosolic Ca(2+). We tested this hypothesis in RIN-m5F cells, which express predominantly the type III rec...

journal_title：Biochemical and biophysical research communications

authors： O'Neill AF,Hagar RE,Zipfel WR,Nathanson MH,Ehrlich BE

更新日期：2002-06-14 00:00:00

abstract：:To date, tick-borne flaviviruses responsible for hemorrhagic fever in humans have been isolated in Siberia (Omsk hemorrhagic fever virus), India (Kyasanur Forest disease virus, KFDV), and in Saudi Arabia (Alkhurma virus, ALKV). Prior to this study, only partial coding sequences of these severe pathogens had been deter...

journal_title：Biochemical and biophysical research communications

authors： Charrel RN,Zaki AM,Attoui H,Fakeeh M,Billoir F,Yousef AI,de Chesse R,De Micco P,Gould EA,de Lamballerie X

更新日期：2001-09-21 00:00:00

abstract：:Microglia, brain macrophages, are thought to be the primary target of HIV-1 infection in the brain, because they exclusively express the CD4 antigen which is effectively used for viral entry. The expression of CD4 mRNA in cultured microglia could be detected by the reverse-PCR method. Using this and immunohistochemica...

journal_title：Biochemical and biophysical research communications

authors： Sawada M,Suzumura A,Marunouchi T

更新日期：1992-12-15 00:00:00

abstract：:We examined whether chondroitin sulfates (CSs) exert inhibitory effects on heparanase (Hpse), the sole endoglycosidase that cleaves heparan sulfate (HS) and heparin, which also stimulates chemokine production. Hpse-mediated degradation of HS was suppressed in the presence of glycosaminoglycans derived from a squid car...

journal_title：Biochemical and biophysical research communications

authors： Higashi N,Maeda R,Sesoko N,Isono M,Ishikawa S,Tani Y,Takahashi K,Oku T,Higashi K,Onishi S,Nakajima M,Irimura T

更新日期：2019-11-26 00:00:00

abstract：:Src and Src-family protein-tyrosine kinases are regulatory proteins that play key roles in cell differentiation, motility, proliferation, and survival. The initially described phosphorylation sites of Src include an activating phosphotyrosine 416 that results from autophosphorylation, and an inhibiting phosphotyrosine...

journal_title：Biochemical and biophysical research communications

authors： Roskoski R Jr

更新日期：2005-05-27 00:00:00

abstract：:Two novel alpha-neurotoxins, Oh-6A and Oh-6B, isolated from the king cobra (Ophiophagus hannah) venom, consist of 70 amino acid residues with 10 cysteine residues and share the same amino acid sequences as determined by Edman degradation on the peptide fragments generated from the proteolytic hydrolysates. Their seque...

journal_title：Biochemical and biophysical research communications

authors： Lin SR,Chang LS,Chang CC

更新日期：1999-01-08 00:00:00

abstract：:Administration of streptozotocin (STZ) induces destruction of β-cells and is widely used as an experimental animal model of type I diabetes. In neonatal rat, after low-doses of STZ-mediated destruction of β-cells, β-cells regeneration occurs and reversal of hyperglycemia was observed. However, in neonatal mice, β-cell...

journal_title：Biochemical and biophysical research communications

authors： Kataoka M,Kawamuro Y,Shiraki N,Miki R,Sakano D,Yoshida T,Yasukawa T,Kume K,Kume S

更新日期：2013-01-18 00:00:00

abstract：:A series of C-terminal linear peptides of endothelin (ET)-1 and their N alpha-succinyl (Suc) analogs were synthesized and their binding affinities for the two subtypes of ET receptor, ETA and ETB, in porcine lung membranes were examined. Among the synthetic analogs, Suc-[Glu9,Ala11,15]-ET-1(8-21), IRL 1620, was the mo...

journal_title：Biochemical and biophysical research communications

authors： Takai M,Umemura I,Yamasaki K,Watakabe T,Fujitani Y,Oda K,Urade Y,Inui T,Yamamura T,Okada T

更新日期：1992-04-30 00:00:00

abstract：:The novel cyclic dinucleotide, 3',5'-cyclic diguanylic acid, cGpGp (c-di-GMP), is a naturally occurring small molecule that regulates important signaling mechanisms in prokaryotes. Recently, we showed that c-di-GMP has "drug-like" properties and that c-di-GMP treatment might be a useful antimicrobial approach to atten...

journal_title：Biochemical and biophysical research communications

authors： Karaolis DK,Cheng K,Lipsky M,Elnabawi A,Catalano J,Hyodo M,Hayakawa Y,Raufman JP

更新日期：2005-04-01 00:00:00

abstract：:We report that N-oleoylethanolamine (NOE), widely employed as a ceramidase inhibitor, also inhibits glucosylation of naturally occurring ceramides. When CHP-100 neuroepithelioma cells were exposed for 18h to non-toxic NOE concentrations (i.e. up to 70 microM), basal incorporation of labelled hexose into glucosylcerami...

journal_title：Biochemical and biophysical research communications

authors： Spinedi A,Di Bartolomeo S,Piacentini M

更新日期：1999-02-16 00:00:00

abstract：:Recently, we reported that Lumbricusin, an antimicrobial peptide isolated from earthworm Lumbricus terrestris, enhanced neuronal proliferation and ameliorated motor dysfunction and dopaminergic neurodegeneration. Accumulating evidence suggests that neurodegeneration is the primary pathological feature of acute or chro...

journal_title：Biochemical and biophysical research communications

authors： Seo M,Lee JH,Baek M,Kim MA,Ahn MY,Kim SH,Yun EY,Hwang JS

更新日期：2017-08-26 00:00:00

abstract：:The death of nigral neurons in Parkinson's disease is thought to involve the formation of the endogenous neurotoxin, 5-S-cysteinyl-dopamine. In the present study, we show that the polyphenols, (+)-catechin and caffeic acid, which contain a catechol moiety, inhibit tyrosinase-induced formation of 5-S-cysteinyl-dopamine...

journal_title：Biochemical and biophysical research communications

authors： Vauzour D,Vafeiadou K,Spencer JP

更新日期：2007-10-19 00:00:00

abstract：:The ability to simultaneously isolate intact DNA-free RNA, genomic DNA, and proteins from a biological specimen can be useful in cloning genes and analyzing gene expression. Equilibrium density gradient centrifugation with CsCl is a useful tool for fractionating, quantitatively separating, and characterizing RNA, DNA,...

journal_title：Biochemical and biophysical research communications

authors： Zhang H,Chen HT,Glisin V

更新日期：2003-12-05 00:00:00