Novel cell culture device enabling three-dimensional cell growth and improved cell function.

Abstract:

:A better understanding of cell biology and cell-cell interactions requires three-dimensional (3-D) culture systems that more closely represent the natural structure and function of tissues in vivo. Here, we present a novel device that provides an environment for routine 3-D cell growth in vitro. We have developed a thin membrane of polystyrene scaffold with a well defined and uniform porous architecture and have adapted this material for cell culture applications. We have exemplified the application of this technology by growing HepG2 liver cells on 2- and 3-D substrates. The performance of HepG2 cells grown on scaffolds was significantly enhanced compared to functional activity of cells grown on 2-D plastic. The incorporation of thin membranes of porous polystyrene to create a novel device has been successfully demonstrated as a new 3-D cell growth technology for routine use in cell culture.

authors

Bokhari M,Carnachan RJ,Cameron NR,Przyborski SA

doi

10.1016/j.bbrc.2007.01.105

subject

Has Abstract

pub_date

2007-03-23 00:00:00

pages

1095-100

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)00168-4

journal_volume

354

pub_type

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