Abstract:
:A better understanding of cell biology and cell-cell interactions requires three-dimensional (3-D) culture systems that more closely represent the natural structure and function of tissues in vivo. Here, we present a novel device that provides an environment for routine 3-D cell growth in vitro. We have developed a thin membrane of polystyrene scaffold with a well defined and uniform porous architecture and have adapted this material for cell culture applications. We have exemplified the application of this technology by growing HepG2 liver cells on 2- and 3-D substrates. The performance of HepG2 cells grown on scaffolds was significantly enhanced compared to functional activity of cells grown on 2-D plastic. The incorporation of thin membranes of porous polystyrene to create a novel device has been successfully demonstrated as a new 3-D cell growth technology for routine use in cell culture.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Bokhari M,Carnachan RJ,Cameron NR,Przyborski SAdoi
10.1016/j.bbrc.2007.01.105subject
Has Abstractpub_date
2007-03-23 00:00:00pages
1095-100issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(07)00168-4journal_volume
354pub_type
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