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Software for precise tracking of cell proliferation.

Abstract：

:We have developed a multi-target cell tracking program TADOR, which we applied to a series of fluorescence images. TADOR is based on an active contour model that is modified in order to be free of the problem of locally optimal solutions, and thus is resistant to signal fluctuation and morphological changes. Due to adoption of backward tracing and addition of user-interactive correction functions, TADOR is used in an off-line and semi-automated mode, but enables precise tracking of cell division. By applying TADOR to the analysis of cultured cells whose nuclei had been fluorescently labeled, we tracked cell division and cell-cycle progression on coverslips over an extended period of time.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kurokawa H,Noda H,Sugiyama M,Sakaue-Sawano A,Fukami K,Miyawaki A

doi

10.1016/j.bbrc.2011.12.100

subject

Has Abstract

pub_date

2012-01-20 00:00:00

pages

1080-5

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(11)02309-6

journal_volume

417

pub_type

杂志文章

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