tsBN75 and tsBN423, temperature-sensitive x-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA.

abstract：:1,25(OH)2D3 receptors were studied in whole testes, Sertoli cells, seminiferous tubules, Leydig cells and spermatogonia of adult NMRI mice and SD rats. Specific reversible high affinity binding (KD 1.4 x 10(-10)M; Nmax 72 fmol/mg protein) by a 3.5 S macromolecule was demonstrated in whole testes, Sertoli cells and sem...

journal_title：Biochemical and biophysical research communications

authors： Merke J,Hügel U,Ritz E

更新日期：1985-02-28 00:00:00

abstract：:Terminal Schwann cells (TSCs) that cover motor neuron terminals are known to play important roles in maintaining neuromuscular junctions, as well as in the repair process after nerve injury. However, molecular characteristics of TSCs remain unknown, because of the difficulties in analyzing them due to their paucity. W...

journal_title：Biochemical and biophysical research communications

authors： Oda R,Yaoi T,Okajima S,Kobashi H,Kubo T,Fushiki S

更新日期：2003-09-05 00:00:00

abstract：:Akt/PKB plays a pivotal role in cell survival and proliferation. Previously, we reported that UV-irradiation induces extensive cell death in Akt2(-/-) mouse embryonic fibroblasts (MEFs) while Akt1(-/-) MEFs show cell cycle arrest. Here, we find that Akt1(-/-) MEFs exhibit phenotypic changes characteristics of senescen...

journal_title：Biochemical and biophysical research communications

authors： Jee HJ,Kim HJ,Kim AJ,Bae YS,Bae SS,Yun J

更新日期：2009-06-05 00:00:00

abstract：:In order to characterize the direct involvement of cAMP in the change of osteoblast proliferation by parathyroid hormone (PTH), we employed the diastereoisomers of adenosine 3',5'-cyclic phosphorothioate, Sp-cAMPS and Rp-cAMPS, which have been recently shown to act directly as agonist and antagonist, respectively in t...

journal_title：Biochemical and biophysical research communications

authors： Kano J,Sugimoto T,Fukase M,Fujita T

更新日期：1991-05-31 00:00:00

abstract：:cDNAs were molecularly cloned for proteins specifically expressed in embryo as well as in a chemically induced rat pancreatic B cell tumor in which virally related oncogenes such as v-myc, v-src, v-yes, v-mos and v-kis were previously demonstrated not to be expressed. A plasmid cDNA library consisting of 48,000 indepe...

journal_title：Biochemical and biophysical research communications

authors： Soma G,Kitahara N,Andoh T

更新日期：1984-10-15 00:00:00

abstract：:Monoclonal antibody BB1 was previously shown to recognize the CD80 protein as well as a putative B7.3 molecule, both ligands of the important T-cell receptors CD28 and CTLA-4. We report that BB1 coprecipitated from detergent lysates of human B- and pre-B-cell lines a protein kinase activity, as detected by solid phase...

journal_title：Biochemical and biophysical research communications

authors： Angelisová P,Cerný J,Horejsí V

更新日期：1996-11-12 00:00:00

abstract：:The membrane electroporation-induced inward current (IMEP) in pituitary tumor (GH3) cells was characterized. This current emerges irregularly when membrane hyperpolarizations to -200 mV with a holding potential of -80 mV were elicited. Neither E-4031 (10 μM), glibenclamide (30 μM), nor ZD7288 (30 μM) caused any effect...

journal_title：Biochemical and biophysical research communications

authors： Wu SN,Huang HC,Yeh CC,Yang WH,Lo YC

更新日期：2011-02-18 00:00:00

abstract：:Aminopeptidase A (APA; EC 3.4.11.7) is a transmembrane metalloprotease with several functions in tumor angiogenesis. To investigate the role of APA in the process of ischemia-induced angiogenesis, we evaluated the cellular angiogenic responses under hypoxic conditions and the process of perfusion recovery in the hindl...

journal_title：Biochemical and biophysical research communications

authors： Kubota R,Numaguchi Y,Ishii M,Niwa M,Okumura K,Naruse K,Murohara T

更新日期：2010-11-12 00:00:00

abstract：:Intracellular transport and storage of retinoids were shown to be conducted by fatty acid-binding protein (FABP). When rat liver cytosol was gel filtrated, retinyl palmitate-binding activity was mainly eluted in the fraction with a Mr. of around 14,000, in which both FABP and cellular retinol-binding protein (CRBP) co...

journal_title：Biochemical and biophysical research communications

authors： Fukai F,Kase T,Shidotani T,Nagai T,Katayama T

更新日期：1987-09-30 00:00:00

abstract：:The postnatal development and molecular properties of somatostatin receptor were studied in rat cerebral cortex. With [125I-Tyr11]SRIF as a radiolabeled ligand, the specific ligand binding to crude membrane increased transiently in the early phase of postnatal development and then decreased. This increase of somatosta...

journal_title：Biochemical and biophysical research communications

authors： Kimura N

更新日期：1989-04-14 00:00:00

abstract：:Several fusion proteins of mouse Interleukins (mILs) and the enhanced green fluorescent protein (EGFP) were expressed in fibroblast and epithelial cells. Among these proteins, the mIL-31 derivative was the most efficiently secreted into the medium in a N-glycosylation-dependent manner. From the analysis of deletion mu...

journal_title：Biochemical and biophysical research communications

authors： Akiyama N,Ohno Y,Fukuda T,Manome Y,Saito S

更新日期：2009-04-17 00:00:00

abstract：:The PAX3 transcription factor is the key regulator of melanocyte development during embryogenesis and is also frequently found in melanoma cells. While PAX3 is known to regulate melanocyte differentiation, survival, proliferation and migration during development, it is not clear if its function is maintained in adult ...

journal_title：Biochemical and biophysical research communications

authors： Medic S,Rizos H,Ziman M

更新日期：2011-08-12 00:00:00

abstract：:Opening of high conductance permeability transition pores in mitochondria initiates onset of the mitochondrial permeability transition (MPT). The MPT is a causative event, leading to necrosis and apoptosis in hepatocytes after oxidative stress, Ca(2+) toxicity, and ischemia/reperfusion. CsA blocks opening of permeabil...

journal_title：Biochemical and biophysical research communications

authors： Kim JS,He L,Lemasters JJ

更新日期：2003-05-09 00:00:00

abstract：:Steatosis is an important clinical manifestation of hepatitis C virus (HCV) infection. The molecular mechanisms of HCV-associated steatosis are not well understood. Sterol regulatory element-binding protein-1c (SREBP-1c) is a key transcription factor which activates the transcription of lipogenic genes. Here we showed...

journal_title：Biochemical and biophysical research communications

authors： Xiang Z,Qiao L,Zhou Y,Babiuk LA,Liu Q

更新日期：2010-11-19 00:00:00

abstract：:The nuclear-mitochondrial stringent response was examined in isonuclear rho+, 21S rRNA-containing rho-, and rho o strains of S. cerevisiae. By 30 min after nutritional downshift, nuclear rDNA transcription falls to 15% of control levels congruently in all strains, as assayed via whole-cell RNA or by hybrid selection o...

journal_title：Biochemical and biophysical research communications

authors： McEntee CM,Cantwell R,Thomas LC,Hudson AP

更新日期：1989-10-16 00:00:00

abstract：:The sum of the magnetic anisotropy and polarization contributions to the magnetic shielding constants of the alpha protons is calculated as a function of the torsion angles about the NC alpha (phi) and C alpha C' (psi) bonds of the dipeptide. The results show that the polarization or electric field effect is several f...

journal_title：Biochemical and biophysical research communications

authors： Gresh N,Giessner-Prettre C

更新日期：1990-09-28 00:00:00

abstract：:Lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria, induces strong proinflammatory responses, including the release of cytokines and nitric oxide from macrophage. In this study, we found that a murine macrophage-derived line, RAW264.7, became multinuclear through cell-cell fusion after inc...

journal_title：Biochemical and biophysical research communications

authors： Nakanishi-Matsui M,Yano S,Matsumoto N,Futai M

更新日期：2012-08-24 00:00:00

abstract：:Although compelling data have demonstrated the effectiveness of estrogen replacement therapy for the treatment of accelerated bone loss in postmenopausal osteoporosis and ovariectomized animals, the mechanisms by which estrogens reduce bone resorption remain to be elucidated. To address this issue, in the present stud...

journal_title：Biochemical and biophysical research communications

authors： Zecchi-Orlandini S,Formigli L,Tani A,Benvenuti S,Fiorelli G,Papucci L,Capaccioli S,Orlandini GE,Brandi ML

更新日期：1999-02-24 00:00:00

abstract：:First messengers and viral transfection are the two most common ways to stimulate cells for signal output, although their applications are limited. We investigated mechanisms of inducing neural stem cell differentiation using recombinant ephrin-B2/Fc and found that it acted as a ligand and inhibited endogenous ephrin-...

journal_title：Biochemical and biophysical research communications

authors： Xiaodong H,Zhen H,Min S,Zhiming C,Hongyan J,Chong Z,Xuefeng T,Guohua J

更新日期：2013-10-18 00:00:00

abstract：:The present study aimed to establish a novel efficient nonviral strategy for suicide gene transfer in hepatocellular carcinoma (HCC) in vivo. We employed branched polyethylenimine (PEI) and combined it with Epstein-Barr virus (EBV)-based plasmid vectors. The HCC cells transfected with an EBV-based plasmid carrying the...

journal_title：Biochemical and biophysical research communications

authors： Iwai M,Harada Y,Tanaka S,Muramatsu A,Mori T,Kashima K,Imanishi J,Mazda O

更新日期：2002-02-15 00:00:00

abstract：:Partial 3'-end nucleotide sequence of the pkI gene (GenBank accession No. AF019143) from Photobacterium leiognathi ATCC 25521 has been determined, and the encoded pyruvate kinase I is deduced. Pyruvate kinase I is the key enzyme of glycolysis, which converts phosphoenol pyruvate to pyruvate. Alignment and comparison o...

journal_title：Biochemical and biophysical research communications

authors： Lin JW,Lu HC,Chen HY,Weng SF

更新日期：1997-10-09 00:00:00

abstract：:The frequency of p73 mutation is low in hematologic malignancies as well as solid tumors. Aberrant DNA methylation of multiple promoter associated CpG islands is a frequent phenomenon in acute lymphoblastic leukemia (ALL). In the present study, we scanned for mutations in the exons 4, 5, 6, and 7 of p73 gene. Using PC...

journal_title：Biochemical and biophysical research communications

authors： Sahu GR,Das BR

更新日期：2005-02-18 00:00:00

abstract：:Membrane rafts are small and dynamic regions enriched in sphingolipids, cholesterol, ganglioside GM1 and protein markers like flotillins, forming the flatter domains or caveolins, which are characterized as stable flask-shape invaginations. We explored whether membrane rafts participate in the entry of Trypanosoma cru...

journal_title：Biochemical and biophysical research communications

authors： Barrias ES,Dutra JM,De Souza W,Carvalho TM

更新日期：2007-11-23 00:00:00

abstract：:2H-NMR spectroscopy has been used to study the distribution of 2H atoms in brain lactate, after intraperitoneal injection of 2H2O. Information on brain metabolism was obtained from the ratio [(3-2H) lactate]/[(2-2H)-lactate] which was found very sensitive to the PO2 in the breathing air. The dependence of this ratio o...

journal_title：Biochemical and biophysical research communications

authors： Vianello F,Scarpa M,Viglino P,Rigo A

更新日期：1997-08-28 00:00:00

abstract：:The distribution, labeling and interrelationship of microsomal and lysosomal dolichol and dolichyl-P in rat liver was investigated. After membrane induction with phenobarbital, N-nitrosodiethylamine and diethylhexylphthalate, the amount of microsomal and lysosomal dolichols are modulated independently. Liposomal label...

journal_title：Biochemical and biophysical research communications

authors： Edlund C,Chojnacki T,Dallner G

更新日期：1983-09-30 00:00:00

abstract：:Glucocorticoids inhibit rodent alpha-fetoprotein (AFP) gene activity but stimulate expression of the human homologue. Like human, activity of the AFP promoter from other primates was stimulated by the synthetic glucocorticoid dexamethasone (Dex) in various cell lines. A glucocorticoid responsive element (GRE) is locat...

journal_title：Biochemical and biophysical research communications

authors： Nakabayashi H,Koyama Y,Sakai M,Li HM,Wong NC,Nishi S

更新日期：2001-09-14 00:00:00

abstract：:The intensity of the fluorescence emission of the fluorescent 1,4-dihydropyridine (DHP) derivative felodipine increased upon binding to both isolated cardiac sarcolemma (SLM) and skeletal muscle sarcoplasmic reticulum (SR) preparations, the latter containing SR-transversal tubule junctional diads and triads. The fluor...

journal_title：Biochemical and biophysical research communications

authors： Minarovic I,Mészáros LG

更新日期：1998-03-17 00:00:00

abstract：:We identified 13 transcript isoforms of a trinucleotide-repeat-containing gene, cag-8, that is expressed almost exclusively in the mouse brain. The polypeptide deduced from the cDNA consists of 137 AAs, of which 74 are glutamines. The 130-kb gene is composed of 16 exons, from which at least 13 isoforms with variable U...

journal_title：Biochemical and biophysical research communications

authors： Ji JW,Yang HL,Kim SJ

更新日期：2006-08-11 00:00:00

abstract：:IRE1α (Inositol-requiring enzyme 1 α), an endoplasmic reticulum (ER)-resident sensor for mammalian unfolded protein response, is a type I transmembrane protein which has a bifunctional enzyme containing kinase and RNase domains. Although the luminal domain and cytosolic domain of IRE1α are thought to play crucial role...

journal_title：Biochemical and biophysical research communications

authors： Cho H,Lamarca R,Chan C

更新日期：2012-11-02 00:00:00

abstract：:Lysophosphatidic acid (LPA) receptors belong to G protein-coupled transmembrane receptors (LPA receptors; LPA(1) to LPA(6)). They indicate a variety of cellular response by the interaction with LPA, including cell proliferation, migration and differentiation. Recently, we have reported that constitutive active mutated...

journal_title：Biochemical and biophysical research communications

authors： Kitayoshi M,Kato K,Tanabe E,Yoshikawa K,Fukui R,Fukushima N,Tsujiuchi T

更新日期：2012-06-01 00:00:00