Hepatocyte growth factor increases mitochondrial mass in glioblastoma cells.


:Hepatocyte growth factor/scatter factor (HGF) is a multifunctional growth factor that is linked to the initiation and/or progression of numerous malignancies. HGF also alters cancer cell responses to DNA damaging cytotoxic agents. Many cell responses to Met activation require alterations in metabolic activity but how the metabolic machinery responds to Met activation remains poorly defined. Treating human glioblastoma cells with HGF followed by the topoisomerase inhibitor camptothecin was found to increase the activity per cell of the mitochondrial respiratory chain enzyme succinate-tetrazolium reductase (>80% increase, p < 0.05) and the tricarboxylic acid cycle enzyme succinate dehydrogenase (>25% increase, p < 0.05). Treatment with either HGF or camptothecin alone had no effect on enzyme activity. The mitochondrial enzymatic response to HGF was dose- and time-dependent with the maximum increase occurring in cells pre-treated with 30 ng/ml HGF for 48h prior to camptothecin exposure. This enzymatic response was associated with a concurrent increase in mitochondrial mass of comparable magnitude (approximately 56%, p < 0.05) as measured by fluorescent mitochondrial staining and flow cytometry. The mitochondrial mass response to HGF was prevented by the MAP-kinase pathway inhibitor PD98059 and was unaffected by the phosphatidylinositol 3-kinase inhibitors LY294002 and wortmannin. These findings suggest that HGF influences cell responses to chemotherapeutic stress, in part, by altering mitochondrial functions through a MAP-kinase dependent increase in mitochondrial mass.


Xia S,Laterra J




Has Abstract


2006-07-14 00:00:00














  • BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon heat stress.

    abstract::Bcl2-associated athoanogene (BAG) 3 is a member of the co-chaperone BAG family. It is induced by stressful stimuli such as heat shock and heavy metals, and it regulates cellular adaptive responses against stressful conditions. In this study, we identified a novel role for BAG3 in regulating the nuclear shuttling of HS...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Jin YH,Ahn SG,Kim SA

    更新日期:2015-08-21 00:00:00

  • Probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway.

    abstract::Probiotics exhibit beneficial functions for host homeostasis maintenance. We herein investigated the mechanism by which Lactobacillus brevis-derived poly P exhibited a beneficial function. Immunostaining indicated that poly P was captured in the plasma membrane via integrin β1 in Caco2/bbe cells. The uptake of poly P ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tanaka K,Fujiya M,Konishi H,Ueno N,Kashima S,Sasajima J,Moriichi K,Ikuta K,Tanabe H,Kohgo Y

    更新日期:2015-11-20 00:00:00

  • Pro-fibrotic effect of oxidized LDL in cardiac myofibroblasts.

    abstract::Inflammatory signals associated with cardiac diseases trigger trans-differentiation of cardiac fibroblasts to cardiac myofibroblasts. Cardiac myofibroblasts are the main cell type involved in the development of cardiac fibrosis, a diffuse and disproportionate accumulation of collagen in the myocardium. Although the ro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Villa M,Cerda-Opazo P,Jimenez-Gallegos D,Garrido-Moreno V,Chiong M,Quest AF,Toledo J,Garcia L

    更新日期:2020-04-09 00:00:00

  • Mg2+- or Mn2+-dependent endonuclease activities of human myeloid leukemia cells capable of producing nucleosomal-size DNA fragmentation.

    abstract::The presence of at least two distinct Mg2+- or Mn2+-dependent, Ca2+-independent endonuclease activities was shown in the myeloid leukemia cell line P39. One of them was recovered from nuclear extracts and the other from a cytoplasmic fraction. The molecular size of the former was 30 kDa in both gel filtration and acti...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kawabata H,Anzai N,Masutani H,Hirama T,Hishita T,Dodo M,Masuda T,Yoshida Y,Okuma M

    更新日期:1997-04-07 00:00:00

  • Trophoblast lineage cells derived from human induced pluripotent stem cells.

    abstract:BACKGROUND:During implantation, the blastocyst trophectoderm attaches to the endometrial epithelium and continues to differentiate into all trophoblast subtypes, which are the major components of a placenta. Aberrant trophoblast proliferation and differentiation are associated with placental diseases. However, due to e...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chen Y,Wang K,Chandramouli GV,Knott JG,Leach R

    更新日期:2013-07-12 00:00:00

  • Regulation of cellulolytic genes by McmA, the SRF-MADS box protein in Aspergillus nidulans.

    abstract::Cellobiose triggers the production of two endoglucanases, EglA and EglB, in Aspergillus nidulans. The cellulose responsive element (CeRE) cis-element that is essential for induction has been identified on the eglA promoter, but transcription factors that bind to CeRE have not yet been identified. CeRE contained a cons...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yamakawa Y,Endo Y,Li N,Yoshizawa M,Aoyama M,Watanabe A,Kanamaru K,Kato M,Kobayashi T

    更新日期:2013-02-22 00:00:00

  • Genetic evidence for involvement of two distinct nonhomologous end-joining pathways in repair of topoisomerase II-mediated DNA damage.

    abstract::In vertebrate cells, DNA double-strand breaks are efficiently repaired by homologous recombination or nonhomologous end-joining (NHEJ). The latter pathway relies on Ku (the Ku70/Ku86 heterodimer), DNA-PKcs, Artemis, Xrcc4, and DNA ligase IV (Lig4). Here, we show that a human pre-B cell line nullizygous for Lig4 exhibi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Adachi N,Iiizumi S,So S,Koyama H

    更新日期:2004-06-11 00:00:00

  • Synthesis and in vitro activity of a hormone-diphtheria toxin fragment A hybrid.

    abstract::The synthesis and in vitro biological activity of a hybrid protein composed of intact human chorionic gonadotropin and fragment A of diphtheria toxin in a disulfide conjugate is reported. This hybrid retained greater than 90% of the binding ability of uncoupled hCG and was shown to be specifically toxic to a mouse Ley...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Oeltmann TN

    更新日期:1985-12-17 00:00:00

  • pp120, a substrate of the insulin receptor tyrosine kinase, is associated with phosphatase activity.

    abstract::pp120, a plasma membrane glycoprotein, is phosphorylated on Tyr488 by the insulin receptor tyrosine kinase. This requires basal phosphorylation on Ser503 by cAMP-dependent serine kinase. Phe513, the other tyrosine residue in the intracellular domain, does not undergo insulin-stimulated phosphorylation. Phe488 mutation...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Najjar SM

    更新日期:1998-06-18 00:00:00

  • Protein kinase PKN1 associates with TRAF2 and is involved in TRAF2-NF-kappaB signaling pathway.

    abstract::PKN1 is a fatty acid and Rho-activated serine/threonine protein kinase whose catalytic domain is highly homologous to protein kinase C (PKC) family. In yeast two-hybrid screening for PKN1 binding proteins, we identified tumor necrosis factor alpha (TNFalpha) receptor-associated factor 2 (TRAF2). TRAF2 is one of the ma...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Gotoh Y,Oishi K,Shibata H,Yamagiwa A,Isagawa T,Nishimura T,Goyama E,Takahashi M,Mukai H,Ono Y

    更新日期:2004-02-13 00:00:00

  • Hepcidin inhibition on the effect of osteogenesis in zebrafish.

    abstract::Iron overload, as a risk factor for osteoporosis, can result in the up-regulation of Hepcidin, and Hepcidin knockout mice display defects in their bone microarchitecture. However, the molecular and genetic mechanisms underlying Hepcidin deficiency-derived bone loss remain unclear. Here, we show that hepcidin knockdown...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Jiang Y,Yan Y,Wang X,Zhu G,Xu YJ

    更新日期:2016-07-15 00:00:00

  • Effects of hydroxyl radical and sulfhydryl reagents on the open probability of the purified cardiac ryanodine receptor channel incorporated into planar lipid bilayers.

    abstract::We examine the effect of hydroxyl radical on the ion permeability of the ryanodine receptor, a calcium releasing channel of sarcoplasmic reticulum. The cardiac ryanodine receptor, purified from pig heart, was reconstituted to proteoliposomes and then incorporated into a planar bilayer membrane. A single channel activi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Anzai K,Ogawa K,Kuniyasu A,Ozawa T,Yamamoto H,Nakayama H

    更新日期:1998-08-28 00:00:00

  • Role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, a review dedicated to Dr. Setsuro Ebashi.

    abstract::Myosin light-chain kinase (MLCK) of smooth muscle consists of an actin-binding domain at the N-terminal, the catalytic domain in the central portion, and the myosin-binding domain at the C-terminal. The kinase activity is mediated by the catalytic domain that phosphorylates the myosin light-chain of 20kDa (MLC20), act...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审


    authors: Nakamura A,Xie C,Zhang Y,Gao Y,Wang HH,Ye LH,Kishi H,Okagaki T,Yoshiyama S,Hayakawa K,Ishikawa R,Kohama K

    更新日期:2008-04-25 00:00:00

  • Photochemical intermediate of third rhodopsin-like pigment in Halobacterium halobium by simultaneous illumination with red and blue light.

    abstract::We found new photochemical intermediate of third rhodopsin-like pigment (tR) or slow cycling rhodopsin-like pigment (sR) in Halobacterium halobium, which was produced by simultaneous illumination with red and blue light. This illumination is employed for measurements of negative phototaxis. The formation of this inter...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tomioka H,Kamo N,Takahashi T,Kobatake Y

    更新日期:1984-09-28 00:00:00

  • Contribution of CD14 to endotoxin-induced liver injury may depend on types of macrophage activation in rats.

    abstract::Activated Kupffer cells and hepatic macrophages can produce massive liver necrosis through microcirculatory disturbance due to sinusoidal fibrin deposition. This mechanism is involved in the development of liver injury after endotoxin administration in rats pretreated with heat-killed Propionibacterium acnes (P.acnes)...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Toshima K,Mochida S,Ishikawa K,Matsui A,Arai M,Ogata I,Fujiwara K

    更新日期:1998-05-29 00:00:00

  • Identification and characterization of an endoplasmic reticulum localization motif.

    abstract::Sorting motifs are involved in the transport of diverse proteins. In the present study, we identified a hydrophobic peptide (WRPWRNFWWSIRVPWRRN) that was able to target enhanced green fluorescent protein- or DsRed2-enriched vesicular-like sub-compartments of the endoplasmic reticulum (ER). Analysis of mutation constru...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nie YW,Wang M,Zhang P,Huan L,Liu DJ

    更新日期:2016-04-22 00:00:00

  • Vectors with hidden cloning sites.

    abstract::A general strategy is described for using the cleavage site of restriction enzymes in vectors for cloning regardless of how many sites the given enzymes have in the vector. The application of this method allows one to open any vector at its cloning site with protruding ends which can be compatible with almost every co...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Welker E,Váradi A

    更新日期:2000-05-10 00:00:00

  • Cardioprotection by modulation of mitochondrial respiration during ischemia-reperfusion: role of apoptosis-inducing factor.

    abstract::The transient, reversible blockade of electron transport (BET) during ischemia or at the onset of reperfusion protects mitochondria and decreases cardiac injury. Apoptosis inducing factor (AIF) is located within the mitochondrial intermembrane space. A release of AIF from mitochondria into cytosol and nucleus triggers...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xu A,Szczepanek K,Hu Y,Lesnefsky EJ,Chen Q

    更新日期:2013-06-14 00:00:00

  • Efficient in vitro transduction of naive murine B cells with lentiviral vectors.

    abstract::The aim of this study was to determine the impact of lentiviral transduction on primary murine B cells. Studying B cell activities in vivo or using them for tolerance induction requires that the cells remain unaltered in their biological behavior except for expression of the transgene. As we show here, murine B cells ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Warncke M,Vogt B,Ulrich J,von Laer MD,Beyer W,Klump H,Micheel B,Sheriff A

    更新日期:2004-06-04 00:00:00

  • Effects of metoclopramide on rat spleen lymphocyte proliferation.

    abstract::The effect of metoclopramide on proliferation of spleen lymphocytes in rats was examined. The rate of [3H]thymidine incorporation into DNA was used as an index of lymphocytes proliferation. It was shown that the incorporation of [3H]thymidine into DNA of spleen lymphocytes in the rats treated with metoclopramide was s...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zerek-Melen G,Winczyk K,Kunert-Radek J,Pawlikowski M

    更新日期:1990-09-28 00:00:00

  • Genotoxicity and immunogenicity of DNA-advanced glycation end products formed by methylglyoxal and lysine in presence of Cu2+.

    abstract::The highly reactive electrophile, methylglyoxal (MG), a break down product of carbohydrates, is a major environmental mutagen having potential genotoxic effects. Previous studies have suggested the reaction of MG with free amino groups of proteins forming advanced glycation end products (AGEs). This results in the gen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ahmad S,Moinuddin,Dixit K,Shahab U,Alam K,Ali A

    更新日期:2011-04-15 00:00:00

  • The glutamate transporter GLAST is involved in spinal nociceptive processing.

    abstract::GLAST and GLT-1 are the most abundant glutamate transporters in the CNS and protect neurons from glutamate neurotoxicity. Here, we investigated the role of GLAST in spinal nociceptive processing. GLAST protein expression was not altered after treatment of rats with either formalin or zymosan. Surprisingly, knock-down ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Niederberger E,Schmidtko A,Coste O,Marian C,Ehnert C,Geisslinger G

    更新日期:2006-07-28 00:00:00

  • Type I collagen induces expression of bone morphogenetic protein receptor type II.

    abstract::The extracellular matrix regulates many fundamental cellular processes such as proliferation, migration, and differentiation. Among the ECM components, type I collagen induces endothelial tube formation in vitro. By analysing genes participating in this event, the bone morphogenetic protein receptor-II (BMPR-II) was d...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Regazzoni C,Winterhalter KH,Rohrer L

    更新日期:2001-05-04 00:00:00

  • 2'-Amino-2'-deoxyguanosine is a cofactor for self-splicing in group I catalytic RNA.

    abstract::In investigations on self-splicing in the group I intron of the pre-mRNA from the nrdB gene of bacteriophage T4 it was found that 2'-amino-2'-deoxyguanosine can replace guanosine as cofactor. This is the first guanosine-analogue with a modification in the 2'-position and substantial activity in a group I self-splicing...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Strömberg R,Hahne S,Sjögren AS,Sjöberg BM

    更新日期:1992-03-16 00:00:00

  • Presence of centrin in the human parasite Giardia: a further indication of its ubiquity in eukaryotes.

    abstract::The presence and distribution of the cytoskeletal protein centrin in the lower eukaryote Giardia were determined using a monoclonal antibody to algal centrin. Immunofluorescence shows that centrin is a major component of rodlike structures associated with the flagella and strongly attached to the ventral disc and the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Belhadri A

    更新日期:1995-09-14 00:00:00

  • TcRho1 of Trypanosoma cruzi: role in metacyclogenesis and cellular localization.

    abstract::Here we have investigated the function of TcRho1, a Rho family orthologue from the parasite Trypanosoma cruzi. We have selected parasites overexpressing wild-type TcRho1 and a truncated form of TcRho1 (TcRho1-DeltaCaaX) which is unable to undergo farnesylation and supposed to interfere with recruitment of Rho effector...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: de Melo LD,Nepomuceno-Silva JL,Sant'Anna C,Eisele N,Ferraro RB,Meyer-Fernandes JR,de Souza W,Cunha-e-Silva NL,Lopes UG

    更新日期:2004-10-22 00:00:00

  • Purification and characterization of a novel tetradecapeptide that modulates oesophagus motility in Lymnaea stagnalis.

    abstract::The isolated oesophagus of Lymnaea stagnalis was used as a bioassay preparation to identify peptides that modulate the activity of the oesophagus. Several modulatory messengers were detected from the oesophageal extract. One of these, having an excitatory action, was further purified from both the oesophagus and the c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Li KW,Holling T,de With ND,Geraerts WP

    更新日期:1993-12-30 00:00:00

  • Transplantation of dermal fibroblasts expressing MyoD1 in mouse muscles.

    abstract::Transplantation of normal myoblasts into dystrophic muscles is a potential treatment for Duchenne muscular dystrophy (DMD). However, the success of such grafts is limited by the immune system responses. To avoid rejection problems, autologous transplantation of the patient's corrected myoblasts has been proposed. Regr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Huard C,Moisset PA,Dicaire A,Merly F,Tardif F,Asselin I,Tremblay JP

    更新日期:1998-07-30 00:00:00

  • Eicosapentaenoic acid inhibits oxidation of high density lipoprotein particles in a manner distinct from docosahexaenoic acid.

    abstract::The omega-3 fatty acid eicosapentaenoic acid (EPA) reduces oxidation of ApoB-containing particles in vitro and in patients with hypertriglyceridemia. EPA may produce these effects through a potent antioxidant mechanism, which may facilitate LDL clearance and slow plaque progression. We hypothesize that EPA antioxidant...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sherratt SCR,Mason RP

    更新日期:2018-02-05 00:00:00

  • Sofalcone, a gastric mucosa protective agent, increases vascular endothelial growth factor via the Nrf2-heme-oxygenase-1 dependent pathway in gastric epithelial cells.

    abstract::Sofalcone, 2'-carboxymethoxy-4,4-bis(3-methyl-2-butenyloxy)chalcone, is an anti-ulcer agent that is classified as a gastric mucosa protective agent. Recent studies indicate heat shock proteins such as HSP32, also known as heme-oxygenase-1(HO-1), play important roles in protecting gastrointestinal tissues from several ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shibuya A,Onda K,Kawahara H,Uchiyama Y,Nakayama H,Omi T,Nagaoka M,Matsui H,Hirano T

    更新日期:2010-07-30 00:00:00