Osterix is dispensable for the development of the mouse olfactory bulb.

Abstract:

:Osterix (Osx) has been shown to be an osteoblast-specific transcription factor for bone formation. Recently, it has been reported that Osx is significantly expressed in the mouse olfactory bulb, proving that Osx may play a role in olfactory bulb development, as well as bone development. Here, we studied morphological differences and neuronal cell alterations in the olfactory bulb using an Osx gene-modified mouse model. Although Osx expression was reduced, morphological differences were not observed in the olfactory bulb of Osx heterozygous mice compared with that of wild-type mice. Immunofluorescence using the neuronal marker genes DCX, MAP2, NeuN, and GFAP showed neuronal cell alterations caused by Osx deficiency in the mitral cell layer (MCL) and granule cell layer (GCL) of the olfactory bulb at postnatal stage. The number, morphology, and expression patterns of immature neurons, mature neurons, and astrocytes were identical in both wild-type and Osx heterozygous mice. At the post-embryonic stage, the expression of neuronal markers DCX, Nestin, MAP2, and NeuN were examined in the MCL and GCL of the olfactory bulb in wild-type, Osx heterozygous, and Osx knockout embryos. Both DCX- and Nestin-positive immature neurons, and MAP2- and NeuN-positive mature neurons, revealed a similar expression pattern in all mouse types. These results indicated that olfactory bulb development was not significantly impaired in the absence of Osx. Further study may be necessary to explain the functional properties of the olfactory bulb caused by Osx deficiency.

authors

Park JS,Park GI,Kim JE

doi

10.1016/j.bbrc.2016.07.085

subject

Has Abstract

pub_date

2016-09-09 00:00:00

pages

110-115

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(16)31207-4

journal_volume

478

pub_type

杂志文章
  • Proliferation of hematopoietic cells is accompanied by suppressed expression of heat shock protein 70.

    abstract::In this study, we have examined the synthesis of heat shock protein (HSP70) in leukemia cells from acute myelogenous leukemia (AML) patients and in mononuclear cells from normal individuals, before and after growth factor stimulation. We have shown that the HSP70 protein was expressed in these cells in the absence of ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(92)90544-u

    authors: Zhang W,Drach J,Andreeff M,Deisseroth A

    更新日期:1992-03-16 00:00:00

  • HSP70 interacts with Rheb, inhibiting mTORC1 signaling.

    abstract::The small GTPase Rheb binds and activates mTORC1, which plays a pivotal role in diverse cellular physiologies. To increase our understanding of how Rheb regulates mTORC1 signaling, we set out to identify Rheb binding proteins using shotgun proteomics approaches. In this study, we characterized HSP70, one of the identi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2020.07.053

    authors: Ryu HH,Ha SH

    更新日期:2020-12-17 00:00:00

  • Vesicular monoamine transporter in microvesicles from bovine posterior pituitaries is immunologically similar to but distinct from the chromaffin granule counterpart in its sensitivities to 1-methyl-4-phenylpyridinium and histamine.

    abstract::The microvesicles (MVs) in bovine posterior pituitaries contain the reserpine-sensitive vesicular monoamine transporter (Moriyama et al. (1995) J. Biol. Chem. 270, 11424-11429). An antibody against the N-terminal region of the monoamine transporter from bovine chromaffin granules recognized a polypeptide in the MVs wi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.0675

    authors: Moriyama Y,Yamada H

    更新日期:1996-04-25 00:00:00

  • Insulin or bFGF and C2 ceramide increase newborn rat retinal ganglion cell survival rate.

    abstract::Treatment of RGCs with insulin or C2 ceramide alone increased survival rate by 30%. Adding both insulin and C2 ceramide increased survival rate by 80%. Protein phosphatase 2A (PP2A) inhibitor okadaic acid (OA) eliminated the effect of C2 ceramide, but not that of insulin. Protein kinase inhibitor K252a decreased the e...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(03)00012-3

    authors: Ito M

    更新日期:2003-02-07 00:00:00

  • Non-electrostatic binding and self-association of amyloid beta-peptide on the surface of tightly packed phosphatidylcholine membranes.

    abstract::Self-association of amyloid beta-peptide (Abeta) is considered to be an initial step in the development of Alzheimer's disease and is known to be promoted by negatively charged lipid membranes. We have examined the possibility of non-electrostatic Abeta-membrane interaction by using neutral phosphatidylcholine lipids....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.08.093

    authors: Yoda M,Miura T,Takeuchi H

    更新日期:2008-11-07 00:00:00

  • Simultaneous assessment of the effects of exonic mutations on RNA splicing and protein functions.

    abstract::To simultaneously assess the effects of exonic mutations on RNA splicing and protein functions, we report here an intron-inclusive cDNA (Intinc) expression system. As a test model, twenty-four mutations in exon 9 of the phenylalanine hydroxylase (PAH) gene were examined in an Intinc expression plasmid composed of the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.06.072

    authors: Ho PY,Huang MZ,Fwu VT,Lin SC,Hsiao KJ,Su TS

    更新日期:2008-09-05 00:00:00

  • An unusual C-H...O hydrogen bond mediated reversal of polypeptide chain direction in a synthetic peptide helix.

    abstract::An unusual C-terminal conformation has been detected in a synthetic decapeptide designed to analyze the stereochemistry of helix termination in polypeptides. The crystal structure of the decapeptide Boc-Leu-Aib-Val-Ala-Leu-Aib-Val-(D)Ala-(D)Leu-Aib-OMe reveals a helical segment spanning residues 1-7 and helix terminat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2000.3026

    authors: Aravinda S,Shamala N,Pramanik A,Das C,Balaram P

    更新日期:2000-07-14 00:00:00

  • Meprin A and meprin alpha generate biologically functional IL-1beta from pro-IL-1beta.

    abstract::The present study demonstrates that both oligomeric metalloendopeptidase meprin A purified from kidney cortex and recombinant meprin alpha are capable of generating biologically active IL-1beta from its precursor pro-IL-1beta. Amino-acid sequencing analysis reveals that meprin A and meprin alpha cleave pro-IL-1beta at...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.12.161

    authors: Herzog C,Haun RS,Kaushal V,Mayeux PR,Shah SV,Kaushal GP

    更新日期:2009-02-20 00:00:00

  • Prediction of structurally conserved regions of D-specific hydroxy acid dehydrogenases by multiple alignment with formate dehydrogenase.

    abstract::We propose a multiple alignment of the sequence of formate dehydrogenase with the D-specific 2-hydroxy acid dehydrogenases family. Structurally conserved regions are predicted for those sequences corresponding to important regions of the catalytic and the coenzyme binding domains defined from the known three-dimension...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.1398

    authors: Vinals C,Depiereux E,Feytmans E

    更新日期:1993-04-15 00:00:00

  • Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta).

    abstract::Intestinal N-acetylglucosamine 6-O-sulfotransferase (I-GlcNAc6ST, GST-4alpha) and corneal N-acetylglucosamine 6-O-sulfotransferases (C-GlcNAc6ST, GST-4beta) are two highly homologous GlcNAc 6-O-sulfotransferase isozymes encoded by two intronless open reading frames that reside approximately 50 kb apart on human chromo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.4668

    authors: Bartes A,Bhakta S,Hemmerich S

    更新日期:2001-04-13 00:00:00

  • Ceramide induces neuronal apoptosis through the caspase-9/caspase-3 pathway.

    abstract::C(2)-ceramide, a cell-permeable analog of ceramide, caused cell death in cultured rat cortical neuronal cells. C(2)-ceramide-induced neuronal loss was accompanied by upregulation of caspase-3 activity, measured by cleavage of its fluorogenic substrate Ac-DEVD-AMC. Similar results were obtained when cortical neuronal c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(02)02593-7

    authors: Movsesyan VA,Yakovlev AG,Dabaghyan EA,Stoica BA,Faden AI

    更新日期:2002-11-29 00:00:00

  • Characterization of rodent epidermal growth factor receptor transcripts using a mouse genomic probe.

    abstract::Two genomic DNA clones encoding part of the mouse epidermal growth factor (EGF) receptor were isolated: an 8.0 kb insert that hybridized with the human EGF receptor kinase domain; and a 2.4 kb insert that mapped between the kinase and 3' autophosphorylation domains of the human EGF receptor gene. The 2.4 kb insert was...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(86)80158-9

    authors: Hung MC,Thompson KL,Chiu IM,Rosner MR

    更新日期:1986-12-30 00:00:00

  • Analysis of Drosophila yellow-B cDNA reveals a new family of proteins related to the royal jelly proteins in the honeybee and to an orphan protein in an unusual bacterium Deinococcus radiodurans.

    abstract::The yellow locus in Drosophila is involved in both cuticle development and behaviour. However, the function of the encoded protein is unknown. Here we have characterised the sequence and expression pattern of a new Drosophila gene, designated yellow-B, encoding a 453-amino-acid protein that is 57% identical to Yellow....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2000.2506

    authors: Maleszka R,Kucharski R

    更新日期:2000-04-21 00:00:00

  • Selenoprotein S is required for clearance of C99 through endoplasmic reticulum-associated degradation.

    abstract::Amyloid beta precursor protein (APP) is normally cleaved by α-secretase, but can also be cleaved by β-secretase (BACE1) to produce C99 fragments in the endoplasmic reticulum (ER) membrane. C99 is subsequently cleaved to amyloid β (Aβ), the aggregation of which is known to cause Alzheimer's disease. Therefore, C99 remo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.03.060

    authors: Jang JK,Park KJ,Lee JH,Ko KY,Kang S,Kim IY

    更新日期:2017-04-29 00:00:00

  • Vaccine based on antibody-dependent cell-mediated cytotoxicity epitope on the H1N1 influenza virus increases mortality in vaccinated mice.

    abstract::Antibody-dependent cell-mediated cytotoxicity bridges humoral immunity and cellular immunity. Thus vaccine candidates which can elicit both broadly neutralizing antibodies and potent antibody-dependent cell-mediated cytotoxicity (ADCC) are recommended. Previously, a panel of functional epitopes that can elicit ADCC ef...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.07.129

    authors: Wang J,Liu M,Ding N,Li Y,Shao J,Zhu M,Xie Z,Sun K

    更新日期:2018-09-10 00:00:00

  • Necrostatin-7 suppresses RANK-NFATc1 signaling and attenuates macrophage to osteoclast differentiation.

    abstract::Osteoclasts play a crucial role in osteolytic bone diseases, such as osteoporosis, rheumatoid arthritis, periodontitis, Paget's disease of bone and bone metastatic tumors. Therefore, controlling osteoclast differentiation and function has been considered a promising therapeutic strategy. Here, we show that necrostatin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.05.153

    authors: Fuji H,Ohmae S,Noma N,Takeiri M,Yasutomi H,Izumi K,Ito M,Toyomoto M,Iwaki S,Takemoto K,Seo S,Taura K,Hida S,Aoyama M,Ishihama Y,Hagiwara M,Takeda N,Hatano E,Iwaisako K,Uemoto S,Asagiri M

    更新日期:2018-09-05 00:00:00

  • The human tumour suppressor LATS1 is activated by human MOB1 at the membrane.

    abstract::Downregulation of the LATS1 tumour suppressor protein kinase contributes to tumour formation in mammals and flies. Strikingly, the tumour suppressor activity depends on the interaction with Dmob (Drosphila Mps1-One binder) in Drosophila melanogaster. Recently, human LATS1 was reported to interact with human MOB1 (hMOB...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.03.244

    authors: Hergovich A,Schmitz D,Hemmings BA

    更新日期:2006-06-23 00:00:00

  • Structural analysis of a putative SAM-dependent methyltransferase, YtqB, from Bacillus subtilis.

    abstract::S-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTases) methylate diverse biological molecules using a SAM cofactor. The ytqB gene of Bacillus subtilis encodes a putative MTase and its biological function has never been characterized. To reveal the structural features and the cofactor binding mode of YtqB,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.03.026

    authors: Park SC,Song WS,Yoon SI

    更新日期:2014-04-18 00:00:00

  • Probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway.

    abstract::Probiotics exhibit beneficial functions for host homeostasis maintenance. We herein investigated the mechanism by which Lactobacillus brevis-derived poly P exhibited a beneficial function. Immunostaining indicated that poly P was captured in the plasma membrane via integrin β1 in Caco2/bbe cells. The uptake of poly P ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.09.159

    authors: Tanaka K,Fujiya M,Konishi H,Ueno N,Kashima S,Sasajima J,Moriichi K,Ikuta K,Tanabe H,Kohgo Y

    更新日期:2015-11-20 00:00:00

  • PAAn-1b and PAAn-E: two phosphorothioate antisense oligodeoxynucleotides inhibit human aromatase gene expression.

    abstract::Estrogen-dependent diseases, especially breast cancers, are frequently treated with aromatase inhibitors. Another more recent strategy is the antisense technology. In this study, after predicting aromatase mRNA secondary structure, we describe the design, the efficiency, and the toxicity of two antisense phosphorothio...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1998.9683

    authors: Auvray P,Sourdaine P,Séralini GE

    更新日期:1998-12-09 00:00:00

  • NAD+ repletion prevents PARP-1-induced glycolytic blockade and cell death in cultured mouse astrocytes.

    abstract::Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear enzyme that is involved in DNA repair and activated by DNA damage. When activated, PARP-1 consumes NAD(+) to form ADP-ribose polymers on acceptor proteins. Extensive activation of PARP-1 leads to glycolytic blockade, energy failure, and cell death. These events have ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(03)01483-9

    authors: Ying W,Garnier P,Swanson RA

    更新日期:2003-09-05 00:00:00

  • AtVps11 is essential for vacuole biogenesis in embryo and participates in pollen tube growth in Arabidopsis.

    abstract::Vacuoles are multiple functional and essential organelles in plants. Studies in Saccharomyces cerevisiae had identified a tethering factor HOPS (Homotypic Fusion and Vacuolar Protein Sorting) complex that plays a critical role in vacuole biogenesis. The HOPS complex consists of four core subunits (Vps11, Vps16, Vps18 ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.07.059

    authors: Tan X,Wei J,Li B,Wang M,Bao Y

    更新日期:2017-09-23 00:00:00

  • Synthesis and processing of killer toxin from Ustilago maydis virus P4.

    abstract::The synthesis of toxin protein from Ustilago maydis virus (UmV) strain P4 was studied in vitro and in vivo. The protein synthesized in vitro and in vivo has a molecular weight of approximately 30 kd whereas the native toxin has a molecular weight of about 12 kd. In the presence of protease inhibitors and glycosylation...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(87)90379-2

    authors: Podila GK,Bozarth RF,Flurkey WH

    更新日期:1987-12-16 00:00:00

  • Troglitazone increases expression of E-cadherin and claudin 4 in human pancreatic cancer cells.

    abstract::We examined the effects of troglitazone on expression of E-cadherin and claudin 4 in human pancreatic cancer cells. Troglitazone dose-dependently increased expression of E-cadherin and claudin 4 mRNA and protein in PK-1 cells. Snail, Slug and ZEB1, mRNAs were not changed by troglitazone, indicating that these three tr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.01.134

    authors: Kumei S,Motomura W,Yoshizaki T,Takakusaki K,Okumura T

    更新日期:2009-03-13 00:00:00

  • A serine point mutation in the adenosine A2AR C-terminal tail reduces receptor heteromerization and allosteric modulation of the dopamine D2R.

    abstract::Evidence exists that the adenosine receptor A(2A)R and the dopamine receptor D(2)R form constitutive heteromers in living cells. Mass spectrometry and pull-down data showed that an arginine-rich domain of the D(2)R third intracellular loop binds via electrostatic interactions to a specific motif of the A(2A)R C-termin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.02.168

    authors: Borroto-Escuela DO,Marcellino D,Narvaez M,Flajolet M,Heintz N,Agnati L,Ciruela F,Fuxe K

    更新日期:2010-03-26 00:00:00

  • Potent ectopic bone-inducing activity of bone morphogenetic protein-4/7 heterodimer.

    abstract::We have purified and characterized recombinant Xenopus bone morphogenetic proteins (xBMPs): homodimers of xBMP-4, 7 and heterodimers (xBMP-4/7) produced by a baculovirus expression system. Highly purified xBMPs had homogeneous NH2-termini predicted from a consensus motif, Arg-X-X-Arg, while they possessed diverse suga...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.1712

    authors: Aono A,Hazama M,Notoya K,Taketomi S,Yamasaki H,Tsukuda R,Sasaki S,Fujisawa Y

    更新日期:1995-05-25 00:00:00

  • Two domains of the epidermal growth factor receptor are involved in cytoskeletal interactions.

    abstract::Epidermal growth factor receptor can interact directly with F-actin through an actin-binding domain. In the present study, a mutant EGFR, lacking a previously identified actin-binding domain (ABD 1), was still able to bind elements of the cytoskeleton. A second EGFR actin-binding domain (ABD 2) was identified in the r...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.03.138

    authors: Song W,Wu J,Ge G,Lin Q

    更新日期:2008-06-13 00:00:00

  • A rat model of human FENIB (familial encephalopathy with neuroserpin inclusion bodies).

    abstract::FENIB (familial encephalopathy with neuroserpin inclusion bodies) is caused by intracellular accumulation/polymerization of mutant neuroserpins in the endoplasmic reticulum (ER). Transgenic rats overexpressing megsin (Tg meg), a newly identified serine protease inhibitor (serpin), demonstrated intraneuronal periodic-a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.06.016

    authors: Takano K,Kitao Y,Inagi R,Momoi T,Matsuyama T,Miyata T,Yoneda Y,Iso H,Stern DM,Hori O,Ogawa S

    更新日期:2006-08-04 00:00:00

  • Regulation of c-myc through intranuclear localization of its RNA subspecies.

    abstract::We used fluorescence in situ hybridization (FISH) to detect c-myc RNA subspecies in human COLO 320DM tumor cells. Although the FISH procedure removed the majority of RNAs from the nucleolus, c-myc RNA continued to be detected in both the nucleoplasm and nucleolus. This finding suggests stable association between c-myc...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.05.201

    authors: Shimizu N,Kawamoto JK,Utani K

    更新日期:2007-08-03 00:00:00

  • Identification and characterisation of a nuclear localisation signal in the SMN associated protein, Gemin4.

    abstract::Gemin4 is a ubiquitously expressed multifunctional protein that is involved in U snRNP assembly, apoptosis, nuclear/cytoplasmic transportation, transcription, and RNAi pathways. Gemin4 is one of the core components of the Gemin-complex, which also contains survival motor neuron (SMN), the seven Gemin proteins (Gemin2-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.07.113

    authors: Lorson MA,Dickson AM,Shaw DJ,Todd AG,Young EC,Morse R,Wolstencroft C,Lorson CL,Young PJ

    更新日期:2008-10-10 00:00:00