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The overexpression of insulin receptor makes CHO cells resistant to the action of IGF-1: role of IRS-1.

Abstract：

:We investigated the influence of the relative abundance of insulin and IGF-1 receptors on cellular growth, by measuring the stimulation of c-fos expression and of H3-thymidine incorporation into DNA by insulin and IGF-1 in CHO cells overexpressing insulin Receptor (CHO-IR). We found that CHO-IR cells were resistant to the action of IGF-1, but were more responsive to insulin, compared to parental clone. This result suggest the presence of a limiting step, distal to the IGF-1 receptor, in the transduction pathway. To address this question we measured the IGF-1 effect on c-fos expression in CHO-IR cells, transiently transfected with the cDNA for IRS-1, the common intracellular substrate for both insulin and IGF-1 receptors (CHO-IR/IRS-1 cells). In these cells IGF-1 stimulated a 10 fold higher c-fos expression compared to CHO-IR cells. These results suggest that the abundance of IRS-1, relative to the number of insulin and IGF-1 receptors, represents a limiting step for the intracellular transduction of insulin and IGF-1 biological messages.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Maggi D,Laurino C,Andraghetti G,Cordera R

doi

10.1006/bbrc.1994.2721

subject

Has Abstract

pub_date

1994-11-30 00:00:00

pages

693-9

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(84)72721-5

journal_volume

205

pub_type

杂志文章

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