Purification and partial sequencing of human placental alkaline phosphatase.

abstract：:Reactive oxygen species such as hydrogen peroxide (H(2)O(2)) have taken center stage as bona fide second messengers in various signaling pathways. Here, we report the synthesis, metabolic fate, and effectiveness in modulating such pathways of a Tat-catalase conjugate. Incubation of L2 cells with Tat-catalase greatly i...

journal_title：Biochemical and biophysical research communications

authors： Watanabe N,Iwamoto T,Bowen KD,Dickinson DA,Torres M,Forman HJ

更新日期：2003-03-28 00:00:00

abstract：:Heat shock proteins are detectable in human atherosclerotic plaques, especially in endothelial cells. In this report we show by immunofluorescence that incubation "in vitro" with OxLDL is a stress capable of inducing the expression of heat shock protein 70 in both the EAhy-926 cell line and human umbilical vein endoth...

journal_title：Biochemical and biophysical research communications

authors： Zhu W,Roma P,Pellegatta F,Catapano AL

更新日期：1994-04-15 00:00:00

abstract：:Fish oil, which contains omega-3 fatty acids mainly in the form of triglycerides, has benefits for reducing breast cancer risk, similar to tamoxifen action. However, it remains to be elucidated whether the combination of omega-3 free fatty acid (ω-3FFA) with tamoxifen leads to improved treatment in breast cancer. In t...

journal_title：Biochemical and biophysical research communications

authors： Wu S,Guo Y,Wu Y,Zhu S,He Z,Chen YQ

更新日期：2015-04-03 00:00:00

abstract：:We previously reported that fucoidan, a dietary fiber purified from seaweed, inhibited IgE production by B cells in vitro. In this study, we examined the effect of fucoidan on IgE production in vivo. The OVA-induced increase of plasma IgE was significantly suppressed when fucoidan was intraperitoneally, but not orally...

journal_title：Biochemical and biophysical research communications

authors： Yanase Y,Hiragun T,Uchida K,Ishii K,Oomizu S,Suzuki H,Mihara S,Iwamoto K,Matsuo H,Onishi N,Kameyoshi Y,Hide M

更新日期：2009-09-25 00:00:00

abstract：:Alpha-crystallin high-molecular-weight (HMW) aggregates can be formed in vitro by many mechanisms, but the mechanism of in vivo aggregation has not been clearly established. HMW and LMW (low-molecular-weight) alpha-crystallins were isolated from human lenses 50-60 years of age and some spectroscopic measurements were ...

journal_title：Biochemical and biophysical research communications

authors： Liang JJ,Akhtar NJ

更新日期：2000-08-28 00:00:00

abstract：:Our knowledge at present on the structure of acrosome-reaction inducing substance (ARIS) in the jelly coat of starfish eggs is summarized. ARIS ia a proteoglycan-like molecule consisting of very long, linear, and highly sulfated glycans and three ARIS proteins, ARIS1-3. Detailed structures of the major glycan of ARIS ...

journal_title：Biochemical and biophysical research communications

authors： Hoshi M,Moriyama H,Matsumoto M

更新日期：2012-08-31 00:00:00

abstract：:Idiopathic epilepsy is characterized by seizures without a clear etiology and is believed to have a strong genetic component but exhibits a complex inheritance pattern. Genetic factors seem to confer a low seizure threshold to susceptible individuals and thereby enhance epileptogenesis. However, the identity of suscep...

journal_title：Biochemical and biophysical research communications

authors： Bae YS,Chung W,Han K,Park KY,Kim H,Kim E,Kim MH

更新日期：2013-04-05 00:00:00

abstract：:The coding sequence of type II phospholipase A2 from human placenta was cloned in a bovine papilloma virus-derived eukaryotic expression vector under the control of the metallothionein promoter. Stably transfected C127 mouse fibroblast lines were obtained with this vector. These transfected cells overexpressed a funct...

journal_title：Biochemical and biophysical research communications

authors： Pernas P,Masliah J,Olivier JL,Salvat C,Rybkine T,Bereziat G

更新日期：1991-08-15 00:00:00

abstract：:Loaded under whole cell patch-clamp configuration, the caged Ca2+ molecule DM-nitrophen was used to increase [Ca2+]i rapidly and reversibly in isolated Deiters cells of the organ of Corti. Photolysis of DM-nitrophen increased [Ca2+]i from resting concentrations of 20-50 nM to values above microM, as measured with the ...

journal_title：Biochemical and biophysical research communications

authors： Dulon D,Blanchet C,Laffon E

更新日期：1994-06-30 00:00:00

abstract：:Human neutrophil cathepsin G and human skin chymase can inactivate bradykinin by cleavage at the carboxy terminal phenylalanyl-arginyl peptide bond of this polypeptide. The mast cell enzyme is far more effective than cathepsin G, the rates of hydrolysis being comparable to that found for angiotensin I to angiotensin I...

journal_title：Biochemical and biophysical research communications

authors： Reilly CF,Schechter NB,Travis J

更新日期：1985-03-15 00:00:00

abstract：:Due to their immune stimulating and apoptosis inducing properties, ligands of the TNF family attract increasing interest as therapeutic proteins. A general limitation of in vivo applications of recombinant soluble TNF ligands is their notoriously rapid clearance from circulation. To improve the serum half life of the ...

journal_title：Biochemical and biophysical research communications

authors： Müller N,Schneider B,Pfizenmaier K,Wajant H

更新日期：2010-06-11 00:00:00

abstract：:The aim of this study was to determine molecular mechanism(s) responsible for the reduced thymidine kinase activity (TK) observed earlier in an arabinosylcytosine (araC) resistant lymphoid cell line (H9-araC cells), which was obtained following continuous cultivation of H9 cells in the presence of 0.5 microM araC. Com...

journal_title：Biochemical and biophysical research communications

authors： Han T,Fernandez M,Sarkar M,Agarwal RP

更新日期：2003-08-01 00:00:00

abstract：:A method for investigating the cellular response of polymorphonuclear leukocytes to various stimuli was introduced using simultaneously native (luminol-independent) and luminol dependent luminescence as an indicator for myeloperoxidase (MPO)-H2O2-halide and O2- mediated reactions. In experimental systems containing lo...

journal_title：Biochemical and biophysical research communications

authors： Roschger P,Graninger W,Klima H

更新日期：1984-09-28 00:00:00

abstract：:Experiments were undertaken to examine the effects of reduced (GSH) and oxidized (GSSG) glutathione on lipid peroxidation of rat liver microsomes. Dependence on microsomal alpha-tocopherol was shown for the GSH inhibition of lipid peroxidation. However, when GSH (5 mM) and GSSG (2.5 mM) were combined in the assay syst...

journal_title：Biochemical and biophysical research communications

authors： Scholz RW,Graham KS,Reddy CC

更新日期：1990-01-30 00:00:00

abstract：:2-keto-3-deoxy-6-phosphogluconate aldolase (E.C. 4.1.2.14) has been purified in two chromatographic steps to 99% purity in 73% overall yield from Azotobacter vinelandii. The pure enzyme is a 70 kD trimeric Class I aldolase, inhibitable by bromopyruvate or pyruvate plus sodium borohydride, with a specific activity of 6...

journal_title：Biochemical and biophysical research communications

authors： Taha TS,Deits TL

更新日期：1994-04-15 00:00:00

abstract：:Post-translational modifications (PTMs) play pivotal roles in controlling the stability and activity of the tumor suppressor p53 in response to distinct stressors. Here we report an unexpected finding of a short chain fatty acid modification of p53 in human cells. Crotonic acid (CA) treatment induces p53 crotonylation...

journal_title：Biochemical and biophysical research communications

authors： Liao P,Bhattarai N,Cao B,Zhou X,Jung JH,Damera K,Fuselier TT,Thareja S,Wimley WC,Wang B,Zeng SX,Lu H

更新日期：2020-04-09 00:00:00

abstract：:A novel human GPCR gene was cloned by PCR with degenerate primers designed from cannabinoid type 1 receptor (CB1) sequences and a full-length clone was isolated by screening of a human genomic DNA library. This gene, termed EDG-3, is highly homologous (51.9 % overall and 69.2 % in seven transmembrane regions) to human...

journal_title：Biochemical and biophysical research communications

authors： Yamaguchi F,Tokuda M,Hatase O,Brenner S

更新日期：1996-10-14 00:00:00

abstract：:Mitochondrial pyruvate carrier (MPC), composed of MPC1 and MPC2, can modulate pyruvate oxidation in mitochondrial and MPC1 expression correlates with poor prognosis of multiple cancers. Here, we reported that MPC1 is acetylated and its main acetylation sites are: K45 and K46. Sirt3 binds to and deacetylates MPC1. High...

journal_title：Biochemical and biophysical research communications

authors： Liang L,Li Q,Huang L,Li D,Li X

更新日期：2015-12-25 00:00:00

abstract：:In addition to inhibiting matrix metalloproteinases, tissue inhibitor of metalloproteinase-1 (TIMP-1) is involved in the regulation of cell growth and survival. To determine its mechanism of action, we investigated effects of TIMP-1 on cell proliferation and survival and signaling pathways induced by TIMP-1 in the hum...

journal_title：Biochemical and biophysical research communications

authors： Lee SJ,Yoo HJ,Bae YS,Kim HJ,Lee ST

更新日期：2003-12-26 00:00:00

abstract：:Human milk Lewis-type (alpha 1,3/4) fucosyltransferase (FT) was separated from the plasma-type by chromatography on bovine IgG glycopep-Sepharose using lactose as the selective eluent and further purified on a column of Sephacryl S-100 HR. The alpha 1,3-FT activity towards 2'-fucosyllactose was found to be associated ...

journal_title：Biochemical and biophysical research communications

authors： Chandrasekaran EV,Rhodes JM,Jain RK,Matta KL

更新日期：1994-01-14 00:00:00

abstract：:MicroRNA-26a is a newly discovered microRNA that has a strong anti-tumorigenic capacity and is capable of suppressing cell proliferation and activating tumor-specific apoptosis. However, whether miR-26a can inhibit the over-growth of lung fibroblasts remains unclear. The relationship between miR-26a and lung fibrosis ...

journal_title：Biochemical and biophysical research communications

authors： Li X,Liu L,Shen Y,Wang T,Chen L,Xu D,Wen F

更新日期：2014-11-28 00:00:00

abstract：:The neural system appears before the vascular system in the phylogenetic tree. During evolution, vascular system generation takes advantage of the pre-existing vascular endothelial growth factor (VEGF) in order to form its networks. Nevertheless, the role of VEGF in neuronal and glial cells is not yet completely under...

journal_title：Biochemical and biophysical research communications

authors： Fusco MA,Wajsenzon IJ,de Carvalho SL,da Silva RT,Einicker-Lamas M,Cavalcante LA,Allodi S

更新日期：2014-05-02 00:00:00

abstract：:The effects of chlorpromazine on either the activity of mouse brain nitric oxide synthase or the induction of lung nitric oxide synthase in mice and rats were studied. Chlorpromazine inhibited the nitric oxide synthase activity in mouse brain cytosol. This effect could be reversed by adding an excess of calmodulin. In...

journal_title：Biochemical and biophysical research communications

authors： Palacios M,Padron J,Glaria L,Rojas A,Delgado R,Knowles R,Moncada S

更新日期：1993-10-15 00:00:00

abstract：:Rab5 is a GTP-binding protein that is crucial for endocytic machinery functions. We previously identified L-plastin as a binding protein for Rab5, using an affinity column with constitutively active Rab5. L- and T-plastin are isoforms of a plastin protein family belonging to actin-bundling proteins that are implicated...

journal_title：Biochemical and biophysical research communications

authors： Hagiwara M,Shinomiya H,Kashihara M,Kobayashi K,Tadokoro T,Yamamoto Y

更新日期：2011-04-15 00:00:00

abstract：:We report the discovery, semi-purification and characterization of a novel Ca2+/calmodulin-dependent protein kinase (peak I kinase) using syntide 2 as a substrate from the rabbit heart. In the study of dependence of peak I kinase on the concentration of calmodulin, half-maximal activation was obtained at approx. 2.0 x...

journal_title：Biochemical and biophysical research communications

authors： Uemura A,Okazaki K,Takesue H,Matsubara T,Hidaka H

更新日期：1995-06-15 00:00:00

abstract：:Polyamine biosynthesis pathway has long been considered an essential drug target for trypanosomatids including Leishmania. S-adenosylmethionine decarboxylase (AdoMetDc) and spermidine synthase (SpdSyn) are enzymes of this pathway that catalyze successive steps, with the product of the former, decarboxylated S-adenosyl...

journal_title：Biochemical and biophysical research communications

authors： Mishra AK,Agnihotri P,Srivastava VK,Pratap JV

更新日期：2015-01-09 00:00:00

abstract：:A two fold stimulation in the incorporation of [3H-methyl] groups from [3H-methyl] methionine into phospholipids was seen in intact pancreatic islets within six minutes of exposure to a glucose concentration that stimulates insulin release. Nonstimulatory sugars, L-glucose and D-galactose, as well as dibutyryl cAMP, d...

journal_title：Biochemical and biophysical research communications

authors： Kowluru A,Rana RS,MacDonald MJ

更新日期：1984-07-31 00:00:00

abstract：:Porins are trimeric proteins in the outer membranes of Gram-negative bacteria. Several of them, among them matrix porin of Escherichia coli, form symmetrically voltage-gated ion channels in planar bilayers. Trimers exhibit negative resistance at potentials larger than +/- 90mV. Here we show that, after two pores withi...

journal_title：Biochemical and biophysical research communications

authors： Buehler LK,Rosenbusch JP

更新日期：1993-01-29 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is a complicated reproductive endocrine disease characterized by hyperandrogenism, polycystic ovaries, and anovulation. Previous studies have revealed that androgen receptors (ARs) are strongly associated with hyperandrogenism and abnormalities in folliculogenesis in patients with PCOS...

journal_title：Biochemical and biophysical research communications

authors： Yu CJ,Liu X,Zhou ZY,Chen XJ,Meng YC,Gu HC,Xu JJ,Ding GL,Liu XM,Sheng JZ,Huang HF

更新日期：2020-02-17 00:00:00

abstract：:A high-pressure stopped-flow apparatus developed in our laboratories provides the capability to use dissolved gaseous reactants at elevated concentrations in solution (in equilibrium with gas pressures up to ca. 30 atm) for measurement of reaction kinetics. We have used this apparatus to follow the reaction of dioxyge...

journal_title：Biochemical and biophysical research communications

authors： Bailey JA,James CA,Woodruff WH

更新日期：1996-03-27 00:00:00