Down-regulation of RalBP1 expression reduces seizure threshold and synaptic inhibition in mice.


:Idiopathic epilepsy is characterized by seizures without a clear etiology and is believed to have a strong genetic component but exhibits a complex inheritance pattern. Genetic factors seem to confer a low seizure threshold to susceptible individuals and thereby enhance epileptogenesis. However, the identity of susceptibility genes and the mechanisms regulating seizure threshold are still poorly understood. Here, we describe that reduced expression of RalBP1, a downstream effector of the small GTPases RalA and RalB, lowers the seizure threshold in mice. The intraperitoneal injection of the chemoconvulsant pentylenetetrazol induced more severe seizures in RalBP1 hypomorphic mice than in their wild-type littermates. The reduction of RalBP1 in the brain has no effect on neuronal excitability, but does decrease the inhibitory synaptic transmission onto CA1 pyramidal neurons. This impaired synaptic inhibition was associated with the loss of GABAergic interneurons in the CA1 subfield of the hippocampus. The present study identifies RalBP1 as a gene regulating the seizure threshold in mice and provides direct evidence for the role of RalBP1 in synaptic inhibition in vivo.


Bae YS,Chung W,Han K,Park KY,Kim H,Kim E,Kim MH




Has Abstract


2013-04-05 00:00:00














  • Presence of several cytokine-like molecules in molluscan hemocytes.

    abstract::The presence of a variety of cytokine-like molecules (IL-1 alpha, IL-1 beta, IL-2, IL-6, TNF-alpha) in the hemocytes of two molluscs, i.e., Planorbarius corneus and Viviparus ater, is reported. These molecules are present only in hemocytes with phagocytic activity. These data, together with recent reports on the prese...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ottaviani E,Franchini A,Franceschi C

    更新日期:1993-09-15 00:00:00

  • Estradiol and pregnenolone sulfate could modulate PMA-stimulated and Ca2+/calmodulin-dependent synaptosomal membrane protein phosphorylation from rat brain in vivo.

    abstract::We have examined the effect of two steroids, pregnenolone sulfate and 17-beta-estradiol, on the activity of two protein kinases: protein kinase C and Ca2+/calmodulin-dependent protein kinase. The activity of the kinases was evaluated as phosphorylation of two synapsins Ia and Ib in cerebral cortex and hippocampus of t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rebas E,Lachowicz A,Lachowicz L

    更新日期:1995-02-15 00:00:00

  • Interaction between cytochrome P450 2B1 and cytochrome bs: inhibition by synthetic peptides indicates a role for P450 residues Lys-122 and Arg-125.

    abstract::Binding of cytochrome b5 to rat cytochrome P450 2B1 was inhibited (by 75%) by a synthetic peptide corresponding to P450 residues 116-134. The role of Lys-122 and Arg-125 were evaluated using peptides in which one or both of these basic residues were replaced with Glu. The Lys-122 substitution nearly abolished while th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Omata Y,Sakamoto H,Robinson RC,Pincus MR,Friedman FK

    更新日期:1994-06-30 00:00:00

  • Platelet-derived growth factor-BB modulates membrane mobility of beta1 integrins.

    abstract::Platelet-derived growth factor (PDGF)-BB elicits a migratory response including reorganization of the actin cytoskeleton in different cell types. Here we have investigated the effects of PDGF-BB stimulation on beta(1) integrin containing focal adhesions in human diploid fibroblasts adhered to collagen type I. Stimulat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ahlén K,Ring P,Tomasini-Johansson B,Holmqvist K,Magnusson KE,Rubin K

    更新日期:2004-01-30 00:00:00

  • Lung oxidative metabolism after exposure to ambient particles.

    abstract::The aim of this work was to study the time course of the oxidative metabolism in mice lung after exposure to ambient particles (ROFA). Swiss mice were intranasally instilled with a ROFA suspension (0.20 mg/kg). Animals were sacrificed 1 or 3 h after the exposure. Eighty percentage of increased oxygen consumption was o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Magnani ND,Marchini T,Tasat DR,Alvarez S,Evelson PA

    更新日期:2011-09-09 00:00:00

  • BDNF is induced by wild-type alpha-synuclein but not by the two mutants, A30P or A53T, in glioma cell line.

    abstract::Parkinson's disease (PD) is one of the most prevalent neurodegenerative diseases but its etiology is unclear. Alpha-synuclein (alpha-SN) is a major component of Lewy bodies and Lewy neurites, and its missense mutations, A30P and A53T, cause familial PD. In PD, alpha-SN-positive glial inclusions are distributed mainly ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kohno R,Sawada H,Kawamoto Y,Uemura K,Shibasaki H,Shimohama S

    更新日期:2004-05-21 00:00:00

  • Systematic time-dependent visualization and quantitation of the neurogenic rate in brain organoids.

    abstract::Organoids mimicking the formation of the brain cortex have been demonstrated to be powerful tools for developmental studies as well as pathological investigations of brain malformations. Here, we report an integrated approach for the quantification of temporal neural production (neurogenic rate) in organoids derived f...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kosodo Y,Suetsugu T,Kobayashi TJ,Matsuzaki F

    更新日期:2017-01-29 00:00:00

  • A 35-kDa co-aggregation factor is a hemin binding protein in Porphyromonas gingivalis.

    abstract::It has been known that Porphyromonas gingivalis has an obligate requirement for hemin or selected heme- or Fe-containing compounds for its growth. In addition, the influence of hemin on the expression of several putative virulence factors produced by this bacterium has also been recently documented; however, the mecha...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shibata Y,Hiratsuka K,Hayakawa M,Shiroza T,Takiguchi H,Nagatsuka Y,Abiko Y

    更新日期:2003-01-10 00:00:00

  • Novel cell culture device enabling three-dimensional cell growth and improved cell function.

    abstract::A better understanding of cell biology and cell-cell interactions requires three-dimensional (3-D) culture systems that more closely represent the natural structure and function of tissues in vivo. Here, we present a novel device that provides an environment for routine 3-D cell growth in vitro. We have developed a th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bokhari M,Carnachan RJ,Cameron NR,Przyborski SA

    更新日期:2007-03-23 00:00:00

  • Kinetic studies of the electron exchange reaction between the octaheme cytochrome c3 (Mr 26000) and the hydrogenase from Desulfovibrio desulfuricans Norway.

    abstract::The octaheme cytochrome c3 (Mr 26000) from Desulfovibrio desulfuricans Norway was studied using cyclic voltammetry at the pyrolytic graphite electrode. The kinetics of reduction of the octaheme cytochrome c3 (Mr 26000) from D. desulfuricans Norway by the Ni-Fe-Se hydrogenase purified from the same organism was investi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Haladjian J,Bianco P,Guerlesquin F,Bruschi M

    更新日期:1991-08-30 00:00:00

  • Phenobarbital and some other liver tumor promoters stimulate DNA synthesis in cultured rat hepatocytes.

    abstract::Hepatocytes isolated from normal adult rats were maintained at sub-confluent density in a defined medium in primary culture. In control cultures with added epidermal growth factor and low concentrations of dexamethasone, the rate of DNA synthesis was initially low but increased after about 30 hours in culture. Additio...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Edwards AM,Lucas CM

    更新日期:1985-08-30 00:00:00

  • Stimulation of free radical generation in human leukocytes by various agents including tumor necrosis factor is a calmodulin dependent process.

    abstract::The mechanism(s) involved in the generation of free radicals in human leukocytes by phorbol myristate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (FMP), lipopolysaccharide (LPS), arachidonic acid (AA), and recombinant-tumor necrosis factor-1-alpha (r-TNF-1 alpha) was investigated. Calmodulin antagonists, chlo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Das UN,Padma M,Sagar PS,Ramesh G,Koratkar R

    更新日期:1990-03-30 00:00:00

  • Semaphorin-3F attracts the growth cone of cerebellar granule cells through cGMP signaling pathway.

    abstract::Growth cone extension is guided by extracellular factors during the brain development but the underlying cellular mechanisms remain largely unclear. Here, we examined the potential function of class-3 semaphorins in cultured cerebellar granule cells. We found neuropilin-2 (NP2), the high-affinity receptor for semaphor...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ding S,Luo JH,Yuan XB

    更新日期:2007-05-18 00:00:00

  • Osteopontin gene expression and protein synthesis in cultured rat mesangial cells.

    abstract::It is controversial whether osteopontin (OP) is expressed in glomeruli and involved in glomerular diseases. We examined whether the OP expression is present at gene and protein levels in cultured rat mesangial cells (MCs). Northern blotting revealed a 1.7 kb OP-mRNA expression in MCs. Fetal calf serum (FCS) and TNF-al...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nagasaki T,Ishimura E,Shioi A,Jono S,Inaba M,Nishizawa Y,Morii H,Otani S

    更新日期:1997-04-07 00:00:00

  • Decrease of calmodulin and actin in the plasma membrane of rat liver cells during proliferative activation.

    abstract::After proliferative activation of rat liver cells in vivo by a partial hepatectomy a decrease of the calmodulin content in the three plasma membrane domains (blood sinusoidal, canalicular and lateral) was observed. At 24 hours after partial hepatectomy calmodulin was found to be 3 fold lower in the sinusoidal and late...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pujades C,Bastos R,Enrich C,Bachs O

    更新日期:1990-12-31 00:00:00

  • Prominin-2 expression increases protrusions, decreases caveolae and inhibits Cdc42 dependent fluid phase endocytosis.

    abstract:BACKGROUND:Membrane protrusions play important roles in biological processes such as cell adhesion, wound healing, migration, and sensing of the external environment. Cell protrusions are a subtype of membrane microdomains composed of cholesterol and sphingolipids, and can be disrupted by cholesterol depletion. Promini...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Singh RD,Schroeder AS,Scheffer L,Holicky EL,Wheatley CL,Marks DL,Pagano RE

    更新日期:2013-05-10 00:00:00

  • Transcriptional activation of SHP by PPAR-gamma in liver.

    abstract::The mechanism of how PPARgamma decrease gluconeogenic gene expressions in liver is still unclear. Since PPARgamma is a transcriptional activator, it requires a mediator to decrease the transcription of gluconeogenic genes. Recently, SHP has been shown to mediate the bile acid-dependent down regulation of gluconeogenic...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim HI,Koh YK,Kim TH,Kwon SK,Im SS,Choi HS,Kim KS,Ahn YH

    更新日期:2007-08-24 00:00:00

  • Phosphorylation of the band 4.1-like proteins of the bovine lens.

    abstract::Crude membrane fractions prepared from the superficial cortical fiber cells of bovine lenses were incubated with [gamma-32P]-ATP. Membrane-associated 4.1-like proteins were then immunoprecipitated and analyzed for 32P-incorporation. Band 4.1-like proteins of 150 kd, 80 kd, and 78 kd were found to be labeled in a time-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Aster JC,Brinn K,Maisel H,Brewer GJ

    更新日期:1985-02-15 00:00:00

  • Molecular cloning of the cytochrome aa3 gene from the archaeon (Archaebacterium) Halobacterium halobium.

    abstract::A novel aa3-type cytochrome oxidase from the extremely halophilic archaeon, Halobacterium halobium, differs significantly from those of other prokaryotic and eukaryotic cytochrome oxidases (Fujiwara, T., Fukumori, Y., and Yamanaka, T. (1989) J. Biochem. 105, 287-292). In the present study, we cloned and sequenced the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Denda K,Fujiwara T,Seki M,Yoshida M,Fukumori Y,Yamanaka T

    更新日期:1991-11-27 00:00:00

  • Determining the 3D structure of human ASC2 protein involved in apoptosis and inflammation.

    abstract::ASC2 structure has been well defined by 1141 NOE experimental restraints. The model consists of five alpha helices. alpha-Helices are connected by short random structure loops. The sole exception is the loop connecting helices 2 and 3, which has a 20-residue length. Folding generally agrees with the folding of recentl...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Espejo F,Patarroyo ME

    更新日期:2006-02-17 00:00:00

  • Crystal structure of BrlR with c-di-GMP.

    abstract::The transcriptional regulator BrlR is a member of the MerR family of multidrug transport activators in Pseudomonas aeruginosa. Recent study indicates that BrlR is a novel 3',5'-cyclic diguanylic acid (c-di-GMP) receptor and can be activated by c-di-GMP. To gain insight into BrlR function, we determined the structure o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Raju H,Sharma R

    更新日期:2017-08-19 00:00:00

  • Expression and characterization of a rabbit liver cytochrome P450 belonging to P450IIB subfamily with the aid of the baculovirus expression vector system.

    abstract::Using baculovirus a cDNA for cytochrome P450 (P450F1) belonging to rabbit P450IIB subfamily was expressed in Spodoptera frugiperda cells, where P450F1 was located on electron-dense structures (derived from the endoplasmic reticulum) present in both the cytoplasm and nucleus. Partially purified P450F1 exhibited absorpt...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ohta D,Matsu-ura Y,Sato R

    更新日期:1991-03-15 00:00:00

  • Isolation of Bcl-2 binding proteins that exhibit homology with BAG-1 and suppressor of death domains protein.

    abstract::The Bcl-2 oncoprotein is a potent inhibitor of apoptosis and is overexpressed in a variety of different malignancies. Bcl-2 function is regulated through heterodimerization with other members of the Bcl-2 protein family. In addition, several proteins that are not members of the Bcl-2 family can bind to Bcl-2, includin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Antoku K,Maser RS,Scully WJ Jr,Delach SM,Johnson DE

    更新日期:2001-09-07 00:00:00

  • A selective splicing variant of hepcidin mRNA in hepatocellular carcinoma cell lines.

    abstract::Hepcidin is a main regulator of iron metabolism, of which abnormal expression affects intestinal absorption and reticuloendothelial sequestration of iron by interacting with ferroportin. It is also noted that abnormal iron accumulation is one of the key factors to facilitate promotion and progression of cancer includi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Toki Y,Sasaki K,Tanaka H,Yamamoto M,Hatayama M,Ito S,Ikuta K,Shindo M,Hasebe T,Nakajima S,Sawada K,Fujiya M,Torimoto Y,Ohtake T,Kohgo Y

    更新日期:2016-08-05 00:00:00

  • Molecular cloning of rat liver glucocorticoid-receptor translocation promoter.

    abstract::We previously found a new endogenous factor in rat liver cytosol, named ATP-stimulated glucocorticoid-receptor translocation promoter (ASTP), that increased the binding of activated glucocorticoid-receptor to nuclei in the presence of ATP. We have cloned and sequenced a cDNA (2989 bp) encoding the ASTP protein. An ope...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Okamoto K,Hirano H,Isohashi F

    更新日期:1993-06-30 00:00:00

  • Unrestricted agonist activity on murine and human cells of a lipopeptide derived from IFN-gamma.

    abstract::We describe the isolation of a synthetic agonist of IFN-gamma (L-mIFN-gamma-CT) active on mouse and human cells. Its biological activity is the result of the ability of the C-terminal extremity of murine IFN-gamma to interact with the intracellular part of IFNgamma-R and the observation that the modification of peptid...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Thiam K,Loing E,Delanoye A,Diesis E,Gras-Masse H,Auriault C,Verwaerde C

    更新日期:1998-12-30 00:00:00

  • Tissue factor pathway inhibitor attenuates ER stress-induced inflammation in human M2-polarized macrophages.

    abstract::Endoplasmic reticulum (ER) stress has been shown to play a key role during the initiation and clinical progression of the cardiovascular diseases, such as atherosclerosis. We have recently shown that expression of tissue factor pathway inhibitor (TFPI) in human monocyte-derived macrophages (MDMs) was induced by choles...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Espada S,Stavik B,Holm S,Sagen EL,Bjerkeli V,Skjelland M,Dahl TB,Espevik T,Kanse S,Sandset PM,Skretting G,Halvorsen B

    更新日期:2017-09-16 00:00:00

  • A three-residue signal confers localization of a reporter protein in the inner nuclear membrane.

    abstract::By alignment to the carboxy-terminal-deduced aa sequence of human cytomegalovirus glycoprotein B (gB), conserved hexameric aa motifs with putative function for localization in the inner nuclear membrane (INM) were identified in the nucleoplasmic tails of herpes simplex virus type 1 gB and of the cellular lamin B recep...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Meyer G,Gicklhorn D,Strive T,Radsak K,Eickmann M

    更新日期:2002-03-08 00:00:00

  • BR140, a novel zinc-finger protein with homology to the TAF250 subunit of TFIID.

    abstract::The Br140 protein was initially identified due to its co-purification with an integrin. We have cloned and sequenced the cDNA corresponding to the Br140 protein. Analysis of the amino acid sequence indicates that Br140 has features characteristic of gene regulatory proteins, including zinc fingers. Furthermore, Br140 ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Thompson KA,Wang B,Argraves WS,Giancotti FG,Schranck DP,Ruoslahti E

    更新日期:1994-02-15 00:00:00

  • Alterations in intermediate filament proteins in rat kidney proximal tubule epithelial cells.

    abstract::Changes in the intermediate filament composition of rat kidney proximal tubule cells in culture have been investigated. The data suggest that differentiated tubular epithelial cells do not express vimentin, but vimentin expression is induced when the cells begin to proliferate in culture. The cultured cells are positi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hatzinger PB,Chen Q,Dong LQ,Stevens JL

    更新日期:1988-12-30 00:00:00