当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Elevated glucose concentrations inhibit DNA synthesis and expression of c-myc in cultured hepatocytes.

Elevated glucose concentrations inhibit DNA synthesis and expression of c-myc in cultured hepatocytes.

Abstract：

:Glucose depressed DNA synthesis in rat hepatocytes in primary culture. As compared to controls cultured with 5.6 mM glucose, maximal (> or = 75%) inhibition was obtained at 20-30 mM, and half-maximal effect at 10-15 mM. Comparison of D- and L-glucose showed that the effect was specific for the D-form. Maximal inhibition required the presence of glucose during the first 24 hours of culture. The expression of the c-myc gene was reduced when the hepatocytes were cultured in the presence of elevated glucose. The responses to epidermal growth factor, insulin and vasopressin, in terms of percentual stimulation of DNA synthesis, were qualitatively similar at 5.6 and 16.8 mM glucose, while glucagon stimulated more strongly when the glucose concentration was increased; glucagon at concentrations > or = 1 nM reversed the inhibition by glucose. 8-Br-cAMP mimicked the effect of glucagon. These results suggest that an increase in the level of glucose depresses hepatocyte DNA synthesis. The effect is associated with lowered expression of the c-myc gene and is counteracted by cAMP.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Dajani OF,Refsnes M,Guren TK,Horn RS,Thoresen GH,Christoffersen T

doi

10.1006/bbrc.1994.2097

subject

Has Abstract

pub_date

1994-08-15 00:00:00

pages

1476-82

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(84)72097-3

journal_volume

202

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Crystal structure of the protein kinase domain of yeast AMP-activated protein kinase Snf1.

    abstract：:AMP-activated protein kinase (AMPK) is a master metabolic regulator, and is an important target for drug development against diabetes, obesity, and other diseases. AMPK is a hetero-trimeric enzyme, with a catalytic (alpha) subunit, and two regulatory (beta and gamma) subunits. Here we report the crystal structure at 2...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.09.181

    authors： Rudolph MJ,Amodeo GA,Bai Y,Tong L

    更新日期：2005-12-02 00:00:00

  • DNA-surfactant interactions: coupled cooperativity in ligand binding leads to duplex stabilization.

    abstract：:The cooperative nature of interaction of cationic surfactants with short oligonucleotides leading to eventual stabilization of DNA duplexes is demonstrated. At submicellar concentrations and DNA:surfactant charge ratios of 0.2 to 0.8, the association of single chain (CTAB) and double chain (DOTAP) surfactants to oligo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1266

    authors： Pattarkine MV,Ganesh KN

    更新日期：1999-09-16 00:00:00

  • Toxoplasma gondii exposes phosphatidylserine inducing a TGF-beta1 autocrine effect orchestrating macrophage evasion.

    abstract：:Toxoplasmosis is a worldwide disease caused by Toxoplasma gondii. Activated macrophages control T. gondii growth by nitric oxide (NO) production. However, T. gondii active invasion inhibits NO production, allowing parasite persistence. Here we show that the mechanism used by T. gondii to inhibit NO production persisti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.09.114

    authors： Seabra SH,de Souza W,Damatta RA

    更新日期：2004-11-12 00:00:00

  • Antisense repression of sucrose phosphate synthase in transgenic muskmelon alters plant growth and fruit development.

    abstract：:To unravel the roles of sucrose phosphate synthase (SPS) in muskmelon (Cucumis melo L.), we reduced its activity in transgenic muskmelon plants by an antisense approach. For this purpose, an 830 bp cDNA fragment of muskmelon sucrose phosphate synthase was expressed in antisense orientation behind the 35S promoter of t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.01.124

    authors： Tian H,Ma L,Zhao C,Hao H,Gong B,Yu X,Wang X

    更新日期：2010-03-12 00:00:00

  • Relative levels of RNA polII subunits differentially affect starvation response in budding yeast.

    abstract：:The Rpb4/7 subcomplex of RNA polymerase II in Saccharomyces cerevisiae is known to play an important role in stress response and stress survival. These two proteins perform overlapping functions ensuring an appropriate cellular response through transcriptional regulation of gene expression. Rpb4 and Rpb7 also perform ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.02.120

    authors： Singh SR,Pillai B,Balakrishnan B,Naorem A,Sadhale PP

    更新日期：2007-04-27 00:00:00

  • Characterization of the 5' flanking region of rat glucokinase gene.

    abstract：:The rat glucokinase (GK) gene containing the first exon was isolated and its 5' flanking region was characterized by the bacterial chloramphenicol acetyltransferase (CAT) assay. A transient expression assay with a series of 5' deletion constructs (-5.5 k to -48) of GK-CAT fusion genes indicated that the 5' flanking se...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91803-2

    authors： Noguchi T,Takenaka M,Yamada K,Matsuda T,Hashimoto M,Tanaka T

    更新日期：1989-11-15 00:00:00

  • High-level expression in Escherichia coli and purification of recombinant plant profilins: comparison of IgE-binding capacity and allergenic activity.

    abstract：:Because of their structural similarity and ubiquitous distribution as actin binding proteins, plant profilins represent important cross-reactive allergens for almost 20% of patients suffering from Type I allergy to pollen and other plant products. The cDNAs coding for three birch profilin variants (Tyr44, Glu47, and A...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1309

    authors： Vrtala S,Wiedemann P,Mittermann I,Eichler HG,Sperr WR,Valent P,Kraft D,Valenta R

    更新日期：1996-09-04 00:00:00

  • Cysteine-106 of DJ-1 is the most sensitive cysteine residue to hydrogen peroxide-mediated oxidation in vivo in human umbilical vein endothelial cells.

    abstract：:Mutation in DJ-1 gene is the cause of autosomal recessive Parkinson's disease, however, its physiological function remains unclear. The isoelectric point of DJ-1 shows an acidic shift after cells are treated with hydrogen peroxide. This suggests that DJ-1 is modified in response to oxidative stress. Here we report the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.03.110

    authors： Kinumi T,Kimata J,Taira T,Ariga H,Niki E

    更新日期：2004-05-07 00:00:00

  • Proatherogenic flow conditions initiate endothelial apoptosis via thrombospondin-1 and the integrin-associated protein.

    abstract：:Recently it has been shown that vascular endothelial cells (EC) are completely devoid of apoptosis if cultivated under a steady laminar flow and that apoptosis is induced by turning off the flow. An autocrine loop of thrombospondin-1 (TSP-1) and the alpha(v)beta(3) integrin/integrin-associated protein (IAP) complex ha...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5314

    authors： Freyberg MA,Kaiser D,Graf R,Buttenbender J,Friedl P

    更新日期：2001-08-10 00:00:00

  • A cryptic protease activity from Trypanosoma cruzi revealed by preincubation with kininogen at low temperatures.

    abstract：:Cysteine proteases have been identified in parasitic protozoa including the causative agent of Chagas' disease Trypanosoma cruzi. T. cruzi lysates subjected to substrate-containing SDS-polyacrylamide gel electrophoresis exhibit major bands of proteolytic activity in the 45-55 kDa molecular mass range (cruzipain activi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7690

    authors： Wiser MF,Lonsdale-Eccles JD,D'Alessandro A,Grab DJ

    更新日期：1997-11-26 00:00:00

  • Receptor binding and Nb2 cell mitogenic activities of glycosylated vs. unglycosylated porcine prolactin.

    abstract：:Purified fractions of glycosylated (pGPrl) and unglycosylated (pUGPrl) porcine prolactin were prepared by affinity chromatography on Concanavalin A-Sepharose. The relative binding activities of these two forms of prolactin for receptors from porcine mammary, adrenal cortex and rabbit mammary, as well as their Nb2 cell...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)81265-8

    authors： Atkinson PR,Seely JE,Klemcke HG,Hughes JP

    更新日期：1988-09-30 00:00:00

  • Functional characterization and genomic organization of the human Na(+)-sulfate cotransporter hNaS2 gene (SLC13A4).

    abstract：:Sulfate plays an essential role in human growth and development. Here, we characterized the functional properties of the human Na(+)-sulfate cotransporter (hNaS2), determined its tissue distribution, and identified its gene (SLC13A4) structure. Expression of hNaS2 protein in Xenopus oocytes led to a Na(+)-dependent tr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.11.102

    authors： Markovich D,Regeer RR,Kunzelmann K,Dawson PA

    更新日期：2005-01-28 00:00:00

  • Hyperphosphorylated p47-phox lost the ability to activate NADPH oxidase in guinea pig neutrophils.

    abstract：:p47-phox is one of the cytosolic activation factors of NADPH oxidase in neutrophils and known to translocate to plasma membranes and function by protein kinase C-phosphorylation. In cytosol fraction, prepared from calyculin A-treated neutrophils, the activity of cytosolic factor to activate NADPH oxidase was more redu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2611

    authors： Yamaguchi M,Saeki S,Yamane H,Okamura N,Ishibashi S

    更新日期：1995-11-02 00:00:00

  • F0F1-ATPase as biosensor to detect single virus.

    abstract：:F(0)F(1)-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody-antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F(0)F(1)-ATPase, which was indicated by F1300, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.02.103

    authors： Liu X,Zhang Y,Yue J,Jiang P,Zhang Z

    更新日期：2006-04-21 00:00:00

  • Spectroscopic evidence for the formation of a transient species during cytochrome P-450scc induced hydroperoxysterol-glycol conversions.

    abstract：:Spectroscopic analysis of the interaction of the epimeric 20-hydroperoxy derivatives of cholesterol with bovine adrenocortical cytochrome P-450scc preparations suggested the formation of a transient species. The intermediate was detected at 4 degrees C and characterized by a minimum at 412 nm in the difference spectru...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91513-9

    authors： Larroque C,van Lier JE

    更新日期：1983-04-29 00:00:00

  • Increased copper toxicity in Saccharomyces cerevisiae lacking VPS35, a component of the retromer and monogenic Parkinson disease gene in humans.

    abstract：:The Saccharomyces cerevisiae gene VPS35 encodes a component of the retromer complex which is involved in vesicle transport from endosomes to the trans-Golgi network. Yeast and human VPS35 orthologs are highly conserved and mutations in human VPS35 cause an autosomal dominant form of late-onset Parkinson disease (PD). ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.05.157

    authors： Sowada N,Stiller B,Kubisch C

    更新日期：2016-08-05 00:00:00

  • Human erythrocyte ankyrin, a cytoskeleton component, generates the p57 membrane proteinase which cleaves C3, the third component of complement.

    abstract：:Human erythrocytes express a p57 membrane serine proteinase which cleaves C3, the third component of complement. Amino acid analysis of the first 20 N-terminal residues of the purified p57 proteinase demonstrated 100% homology with residues 910-929 of erythrocyte ankyrin, a sequence localized in its Mr = 62 kDa fragme...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2481

    authors： Hermann J,Barel M,Frade R

    更新日期：1994-10-28 00:00:00

  • Role of CXC chemokines in the enhancement of LPS-induced neutrophil accumulation in the lung of mice by dexamethasone.

    abstract：:Lipopolysaccharide (LPS)-induced multiple organ injury was mediated in part by a transcription factor, nuclear factor-kappaB (NF-kappaB). Mice were pretreated with dexamethasone (DEX), an inhibitor of NF-kappaB activation, to elucidate its effects on LPS-induced early responses in vivo. Early responses measured 1 h af...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/S0006-291X(02)00573-9

    authors： Aoki K,Ishida Y,Kikuta N,Kawai H,Kuroiwa M,Sato H

    更新日期：2002-06-28 00:00:00

  • Comparative studies of polyhemic cytochromes c isolated from Desulfovibrio vulgaris (Hildenborough) and Desulfovibrio desulfuricans (Norway).

    abstract：:Cytochrome c3 (Mr 26,000) has been characterized in Desulfovibrio vulgaris (Hildenborough) and its properties compared with polyhemic cytochromes c isolated from the same organism and from D. desulfuricans (Norway). It can be described as an octaheme cytochrome c3 constituted of two identical subunits. Absorption spec...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)90047-8

    authors： Loutfi M,Guerlesquin F,Bianco P,Haladjian J,Bruschi M

    更新日期：1989-03-15 00:00:00

  • In situ hybridization patterns of zebrafish homeobox genes homologous to Hox-2.1 and En-2 of mouse.

    abstract：:Vertebrate homeobox genes have been identified on the basis of sequence homology to Drosophila segmentation and homeotic genes. We have analyzed the spatial distribution patterns in hatching larvae of transcripts from two different types of zebrafish (Brachydanio rerio) homeobox genes. Tritiated DNA fragments from one...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80266-3

    authors： Njølstad PR,Fjose A

    更新日期：1988-12-15 00:00:00

  • Oxidative DNA damage by minor metabolites of toluene may lead to carcinogenesis and reproductive dysfunction.

    abstract：:Recently, the concern that toluene might have carcinogenic and reproductive toxic potential has been raised. We investigated the ability of DNA damage by minor metabolites of toluene, methylhydroquinone, and methylbenzoquinone, using (32)P-5'-end-labeled DNA fragments obtained from the human genes. Methylhydroquinone ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1041

    authors： Murata M,Tsujikawa M,Kawanishi S

    更新日期：1999-08-02 00:00:00

  • Activation of calcium-sensing receptor-mediated autophagy in angiotensinII-induced cardiac fibrosis in vitro.

    abstract：:Cardiac fibrosis is one of the primary mechanisms of ventricular remodeling, and there is no effective method for reversal. Activation of calcium sensing receptor (CaSR) has been reported to be involved in the development of myocardial fibrosis, but the molecular mechanism for CaSR activation has not yet been clarifie...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.02.098

    authors： Chi J,Wang L,Zhang X,Fu Y,Liu Y,Chen W,Liu W,Shi Z,Yin X

    更新日期：2018-03-04 00:00:00

  • Role of excess lipoyl dehydrogenase in reconstituted alpha-ketoglutarate dehydrogenase complex of Escherichia coli.

    abstract：:The alpha-ketoglutarate dehydrogenase complex of Escherichia coli can bind up to 12 dimers of dihydrolipoyl dehydrogenase (E3) besides those already present. Maximal activity does not increase, however, when surplus E3 is present. This observation was previously interpreted to mean that the excess enzyme is inactive. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90999-x

    authors： Wagenknecht T,Francis N,DeRosier D

    更新日期：1986-03-28 00:00:00

  • Purification and characterization of mouse CYP27B1 overproduced by an Escherichia coli system coexpressing molecular chaperonins GroEL/ES.

    abstract：:The expression of mouse CYP27B1 in Escherichia coli has been dramatically enhanced by coexpression of GroEL/ES. To reveal the enzymatic properties of CYP27B1, we measured its hydroxylation activity toward vitamin D3 and 1alpha-hydroxyvitamin D3 (1alpha(OH)D3) in addition to the physiological substrate 25(OH)D3. Surpri...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.08.110

    authors： Uchida E,Kagawa N,Sakaki T,Urushino N,Sawada N,Kamakura M,Ohta M,Kato S,Inouye K

    更新日期：2004-10-15 00:00:00

  • Expression of P-glycoprotein in L1210 cells is linked with rise in sensitivity to Ca2+.

    abstract：:L1210/VCR cell line (R) was obtained by adaptation of the L1210 mouse leukaemia cells (S) to vincristine and showed P-glycoprotein (P-gp) mediated multidrug resistance (MDR). R cells were observed to be more sensitive to high external calcium as parental S. More pronounced calcium uptake was observed for R cells. More...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.07.144

    authors： Sulová Z,Orlický J,Fiala R,Dovinová I,Uhrík B,Seres M,Gibalová L,Breier A

    更新日期：2005-09-30 00:00:00

  • The effect of interaction between human urokinase and its competitive inhibitor, N alpha-benzoyl-L-arginine amide, on reduction of a specific SS bond related to enzymatic activity.

    abstract：:The 55- (H-UK) and 36-kDa forms (L-UK) of human urinary urokinase lost most of esterase activity toward acetyl-glycyl-L-lysine methyl ester upon reductive cleavage of 3 SS bonds with dithiothreitol in the presence of the competitive inhibitor, N alpha-benzoyl-L-arginine amide (BAA), bound to polyacrylyl azide with C16...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91526-7

    authors： Miwa N,Obata Y,Suzuki A

    更新日期：1983-04-29 00:00:00

  • Antigenic and size differences between somatic and testicular cytochromes c.

    abstract：:Enzyme-linked immunosorbent assays (ELISA) of somatic (cs) and testicular (ct) cytochromes of rat, mouse, rabbit, and beef with rabbit anti-rat cyt ct antibody exhibited two different antigenic profiles, indicating the presence of two different antigenic structure between cyt ct and cs. SDS-polyacrylamide gel electrop...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80344-8

    authors： Kim IC,Sabourin CL

    更新日期：1986-11-26 00:00:00

  • Monolayer expansion induces an oxidative metabolism and ROS in chondrocytes.

    abstract：:This study tests the hypothesis that articular chondrocytes shift from a characteristically glycolytic to an oxidative energy metabolism during population expansion in monolayer. Bovine articular chondrocytes were cultured in monolayer under standard incubator conditions for up to 14 days. Cellular proliferation, oxyg...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.06.011

    authors： Heywood HK,Lee DA

    更新日期：2008-08-22 00:00:00

  • microRNA-183 plays as oncogenes by increasing cell proliferation, migration and invasion via targeting protein phosphatase 2A in renal cancer cells.

    abstract：:The aim of this study was to investigate the function of miR-183 in renal cancer cells and the mechanisms miR-183 regulates this process. In this study, level of miR-183 in clinical renal cancer specimens was detected by quantitative real-time PCR. miR-183 was up- and down-regulated in two renal cancer cell lines ACHN...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.08.067

    authors： Qiu M,Liu L,Chen L,Tan G,Liang Z,Wang K,Liu J,Chen H

    更新日期：2014-09-12 00:00:00

  • Human salivary eicosanoids: circadian variation.

    abstract：:A circadian rhythm in the concentrations of prostaglandins (PG) E2, PGF2, PGI2 (measured as 6-keto-PGF1 alpha), immunoreactive h hydroxyeicosatetraenoic acids and immunoreactive 6-sulfidopeptide containing leukotrienes in human mixed saliva was observed. The rhythm reflected changes in the absolute amounts of these co...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)90989-0

    authors： Rigas B,Levine L

    更新日期：1983-08-30 00:00:00