Abstract：
:We showed immunohistochemically the localization of 5 alpha-reductase-containing cells in the rat brain, using a rabbit antibody generated against 5 alpha-reductase rat type 1. The antibody was produced by injecting the synthetic peptide corresponding to the amino acids 38-53 of 5 alpha-reductase rat type 1, conjugated to keyhole limpet hemocyanin with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride. Western blot analysis revealed that this antiserum recognized the protein with a molecular weight of 29,000 Da. The immunoreactive cells were distributed throughout the brain and they were preferentially located in the white matter rather than in the grey matter. These cells were mostly small and round and had a few fine processes. The immunoreaction was confined to the cytoplasm and processes. These findings indicate that 5 alpha-reductase rat type 1-containing cells are widely distributed in the rat brain and are located preferentially in the white matter rather than in the grey matter.
journal_name
Brain Resjournal_title
Brain researchauthors
Tsuruo Y,Miyamoto T,Yokoi H,Kitagawa K,Futaki S,Ishimura Kdoi
10.1016/0006-8993(96)00188-6subject
Has Abstractpub_date
1996-05-25 00:00:00pages
207-11issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(96)00188-6journal_volume
722pub_type
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