Abstract：
:HB-GAM (heparin-binding growth-associated molecule) is a secretory, extracellular matrix-associated protein that was isolated by screening for proteins that enhance neurite outgrowth in perinatal rat brain neurons. In the present study we have investigated the possible role of HB-GAM in cell proliferation in the developing rat limb. Exogenously added recombinant HB-GAM was found to inhibit the proliferation of mesenchymal and epithelial cells in cultured limb buds, as demonstrated by bromodeoxyuridine incorporation and by staining for PCNA (proliferating cell nuclear antigen). The inhibitory effect of HB-GAM on cell proliferation was reversed by heparin, suggesting that HB-GAM may bind to a heparin-type carbohydrate epitope that is required for cell proliferation in the developing limb. Endogenous HB-GAM of the developing limb was found to be expressed in a proximal-to-distal pattern, in agreement with the putative role in proliferation arrest and cell differentiation. In addition, double immunostaining of HB-GAM with PCNA showed that in early (Embryonic Day 12) limb mesenchyme HB-GAM was associated mainly with the surface of growth-arrested cells. Furthermore, HB-GAM was associated with the muscle surface, as demonstrated in double immunostaining of HB-GAM with desmin and myosin heavy chain proteins. Coinciding with the onset of synapse formation (Embryonic Day 16), HB-GAM was found in patches on the muscle cell surface in close proximity to nicotinic acetylcholine receptor clusters. This finding is in agreement with a previous study that has suggested a role for HB-GAM in the differentiation of the neuromuscular junction in Xenopus muscle.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Szabat E,Rauvala Hdoi
10.1006/dbio.1996.0199subject
Has Abstractpub_date
1996-08-25 00:00:00pages
77-89issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(96)90199-2journal_volume
178pub_type
杂志文章abstract：:The rabbit sperm membrane autoantigen RSA-1 is a sialoglycoprotein of 13,000 daltons which first appears on the surface of pachytene spermatocytes. Using specific antiserum to RSA-1 the antigen has been localized by immunofluorescence and immunoperoxidase staining. On testicular cells labeled at 37 degrees C, RSA-1 is...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(81)90400-0
更新日期：1981-06-01 00:00:00
abstract：:The 3' penultimate exon (exon 18) of transcripts from the muscle myosin heavy chain (MHC) gene of Drosophila melanogaster is excluded from mRNAs of embryonic and larval muscle, while it is included in mRNAs of adult thoracic muscles. By transforming organisms with the MHC gene 5' end, linked in-frame to the MHC gene 3...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90235-u
更新日期：1991-08-01 00:00:00
abstract：:A fundamental question in biology is how animal segmentation arose during evolution. One particular challenge is to clarify whether segmental ganglia of the nervous system evolved once, twice, or several times within the Bilateria. As close relatives of arthropods, Onychophora play an important role in this debate sin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.08.011
更新日期：2009-11-01 00:00:00
abstract：:Mechanisms coupling growth and metabolism are conserved in Drosophila and mammals. In metazoans, such coupling is achieved across tissue scales through the regulated secretion of chemical messengers such as insulin that control the metabolism and growth of cells. Although the regulated secretion of Insulin like peptid...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.12.008
更新日期：2018-02-01 00:00:00
abstract：:The segmentation of somites from the paraxial mesoderm is a crucial event in vertebrate embryonic development; however, the mechanisms underlying this process are not well understood. In a yeast two-hybrid screen we have identified the novel basic-helix-loop-helix (bHLH) protein cMeso-1 which is expressed in the preso...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8919
更新日期：1998-07-15 00:00:00
abstract：:Monoclonal antibody 4F4, which was raised against a cell suspension of embryonic rat forebrain, reacts with acidic glycolipids and several high-molecular-weight glycoproteins in rodent brain. The major reactive glycolipid is maximally expressed at Embryonic Day 15 (E15) and is no longer detectable at Postnatal Day 14 ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90104-7
更新日期：1987-03-01 00:00:00
abstract：:Regulated changes in the cell cycle underlie many aspects of growth and differentiation. Prior to meiosis, germ cell cycles in many organisms become accelerated, synchronized, and modified to lack cytokinesis. These changes cause cysts of interconnected germ cells to form that typically contain 2(n) cells. In Drosophi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9570
更新日期：2000-02-01 00:00:00
abstract：:The fertilized ascidian egg is thought to be comprised of distinct regions of tissue-specific cytoplasmic determinants. This idea was tested by bisecting fertilized eggs into egg fragments and culturing them until the unoperated controls developed into larvae. Fertilized eggs were bisected using a microsurgical method...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90178-9
更新日期：1988-09-01 00:00:00
abstract：:Previous studies from this laboratory have identified a novel alpha-D-mannosidase on the sperm plasma membranes of several species, including man, which may have a role in fertilization. The polyclonal antibody raised against an isoform of the enzyme purified from rat epididymal fluid was found to cross-react with the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1050
更新日期：1995-02-01 00:00:00
abstract：:The Mesp bHLH genes play a conserved role during segmental patterning of the mesoderm in the vertebrate embryo by specifying segmental boundaries and anteroposterior (A-P) segmental polarity. Here we use a xenotransgenic approach to compare the transcriptional enhancers that drive expression of the Mesp genes within s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.12.038
更新日期：2008-03-15 00:00:00
abstract：:To examine whether the 200-kDa neurofilament protein (200K NFP) is involved in mechanically stabilizing axons, we studied the developmental appearance of immunoreactivity to nonphosphorylated and phosphorylated 200K NFP at the neuromuscular junction. Polyinnervated rat muscle fibers become singly innervated during the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90422-8
更新日期：1988-11-01 00:00:00
abstract：:Unequal first cleavage is characteristic of a diverse group of protostome animals. In the nematode Caenorhabditis elegans, unequal first cleavage is achieved through the interaction of an apparently symmetric mitotic spindle apparatus with a clearly polarized cell cortex. In the clitellate annelid Tubifex tubifex, by ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.12.049
更新日期：2006-04-01 00:00:00
abstract：:T-box family transcription factors play many roles in Metazoan development. Here we characterise Tbx6r, a unique Tbx6 paralogue isolated from the amphibian Xenopus. The evolution and developmental integration of this divergent T-box gene within the vertebrates reveals an unexpected level of plasticity within this cons...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.01.013
更新日期：2010-04-01 00:00:00
abstract：:To examine the conditions of cell composition necessary for formation of a complete tadpole, defect embryos of 35 series were prepared by removing particular cells from 16- and 32-cell embryos of Xenopus laevis. These defect embryos were cultured and their development was examined. Formation of a complete tadpole did ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1223
更新日期：1995-08-01 00:00:00
abstract：:The development of the vertebrate inner ear is a complex process that has been investigated in several model organisms. In this work, we examined genetic interactions regulating early development of otic structures in medaka. We demonstrate that misexpression of Fgf8, Dlx3b and Foxi1 during early gastrulation is suffi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.04.022
更新日期：2007-07-15 00:00:00
abstract：:The t haplotypes are variant forms of the proximal portion of chromosome 17 in the mouse. The t haplotypes alter spermatogenesis and many also contain lethal factors. Although the lethal factors vary between t haplotypes, all t haplotypes have the same effect on sperm, that of altering sperm function in fertilization....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1002
更新日期：1993-01-01 00:00:00
abstract：:Hoxa2 gene is a primary player in regulation of craniofacial programs of head development in vertebrates. Here we investigate the evolution of a Hoxa2 neural crest enhancer identified originally in mouse by comparing and contrasting the fugu hoxa2a and hoxa2b genes with their orthologous teleost and mammalian sequence...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.11.006
更新日期：2016-01-15 00:00:00
abstract：:The universal signal for egg activation at fertilization is a rise in cytoplasmic Ca(2+) with defined spatial and temporal kinetics. Mammalian and amphibian eggs acquire the ability to produce such Ca(2+) signals during a maturation period that precedes fertilization and encompasses resumption of meiosis and progressi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.08.004
更新日期：2004-11-01 00:00:00
abstract：:To gain an understanding of the genes and mechanisms that govern morphogenesis and its evolution, we have analyzed mutations that disrupt this process in a simple model structure, the male tail tip of the rhabditid nematode C. elegans. During the evolution of rhabditid male tails, there have been several independent c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.04.472
更新日期：2006-09-01 00:00:00
abstract：:We examined the effects of ciliary neurotrophic factor (CNTF) and depolarization, two environmental signals that influence noradrenergic and cholinergic function, on neuropeptide expression by cultured sympathetic neurons. Sciatic nerve extract, a rich source of CNTF, increased levels of vasoactive intestinal peptide ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90242-9
更新日期：1992-04-01 00:00:00
abstract：:It is well established that the notochord influences the development of adjacent neural and mesodermal tissue. Involvement of the notochord in the differentiation of the dorsal pancreas has been demonstrated. However, our knowledge of the signals involved in pancreatic development is still incomplete. In order to iden...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9348
更新日期：1999-08-15 00:00:00
abstract：:John W. Saunders, Jr. made seminal discoveries unveiling how chick embryos develop their limbs. He discovered the apical ectodermal ridge (AER), the zone of polarizing activity (ZPA), and the domains of interdigital cell death within the developing limb and determined their function through experimental analysis. Thes...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2016.11.005
更新日期：2017-09-15 00:00:00
abstract：:We have examined the expression of mRNAs encoding five major neurotransmitter-synthesizing enzymes in MAH cells, a clonal cell line derived by retroviral immortalization of a rat embryonic sympathoadrenal progenitor cell. These mRNAs include tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), tryptophan hydro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90313-r
更新日期：1991-11-01 00:00:00
abstract：:The chicken retina is capable of limited regeneration. In response to injury, some Müller glia proliferate and de-differentiate into progenitor cells. However, most of these progenitors fail to differentiate into neurons. The Notch pathway is upregulated during retinal regeneration in both fish and amphibians. Since t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.09.046
更新日期：2007-12-01 00:00:00
abstract：:We previously isolated a p53 cDNA from a Xenopus oocyte library. To determine if p53 has a function in the developmental period, we have studied its expression at the RNA and protein levels during the early development of Xenopus laevis. Two p53 transcripts (3 and 2.2 kb) are expressed from the beginning of Xenopus oo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1230
更新日期：1993-09-01 00:00:00
abstract：:An abundant phosphoprotein with an apparent molecular mass of 65 kDa (p65) has been identified that is enriched in developing new macronuclei (or anlagen) isolated from the holotrichous ciliate, Tetrahymena thermophila. During early stages of macronuclear development, p65 is actively synthesized and deposited into you...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1264
更新日期：1994-10-01 00:00:00
abstract：:In many animals, the first cue for development is transcripts and/or proteins that are provided maternally and are localized at specific regions of fertilized eggs and early embryos. The ascidian is known to exhibit a mosaic mode of development, which is largely dependent on localized maternal factors. In early Ciona ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.05.018
更新日期：2006-08-15 00:00:00
abstract：:We report the patterns of migration of Sertoli cells plated on specific substrata, and the influences of testicular peritubular cells on these processes. Data presented indicate that while peritubular cells readily spread when explanted onto Type I collagen, Sertoli cells do not. A delay of 4 to 6 days occurs after Se...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90114-4
更新日期：1986-01-01 00:00:00
abstract：:We examined the structure, expression, and possible functions of a nanos homologue during gametogenesis and blastogenesis in the colonial ascidian Botryllus primigenus. An approximately 1.3-kb-long cDNA was cloned; it was termed BpNos since the deduced amino acid sequence (288 aa) contained 2 Nanos-like CCHC zinc fing...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.08.023
更新日期：2008-12-01 00:00:00
abstract：:In marine teleosts, the aqp1ab water channel plays a vital role in the development of the pelagic egg phenotype. However, the developmental control of aqp1ab activation during oogenesis remains to be established. Here, we report the isolation of the 5'-flanking region of the teleost gilthead seabream aqp1ab gene, in w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.03.001
更新日期：2013-05-15 00:00:00
