Abstract：
:We previously isolated a p53 cDNA from a Xenopus oocyte library. To determine if p53 has a function in the developmental period, we have studied its expression at the RNA and protein levels during the early development of Xenopus laevis. Two p53 transcripts (3 and 2.2 kb) are expressed from the beginning of Xenopus oogenesis, and the major one (2.2 kb) reaches a level of 7 x 10(5) to 7 x 10(6) transcripts per mature oocyte. After fertilization only the 2.2-kb RNA is detected, but its level decreases and at the neurula stage p53 RNA becomes undetectable. The p53 protein is highly expressed during Xenopus development, in contrast to an undetectable level in Xenopus cells in culture. Most of the p53 protein is synthesized during late oogenesis and a stage VI oocyte contains 7 x 10(11) molecules of p53 protein. This maternal p53 store is maintained at a constant level during Xenopus development, at least until the tadpole stage. This high level of expression is mainly due to stabilization of the p53 protein. Unusually for p53, the protein is strictly located in the cytoplasm of oocytes and this localization might indicate that it is stored in an inactive form at this stage. These data are discussed relative to previous observations made in transgenic mice.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Tchang F,Gusse M,Soussi T,Méchali Mdoi
10.1006/dbio.1993.1230subject
Has Abstractpub_date
1993-09-01 00:00:00pages
163-72issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(83)71230-3journal_volume
159pub_type
杂志文章abstract：:A subset of macrophages that reside in adult tissues originate from the fetal yolk sac, while others derive from circulating monocytes. These ontologically different macrophage subsets have distinct roles in tissue injury responses, with the embryonic population overall having beneficial activity in cardiac repair. He...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.03.014
更新日期：2016-05-15 00:00:00
abstract：:The facial primordia in the chick embryo begin as rounded swellings that surround the primitive mouth and these grow out to form the beak. The control of proximodistal outgrowth is not well understood but may involve similar mechanisms to the limb bud. In order to test this hypothesis, combinations were made between e...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90069-s
更新日期：1992-12-01 00:00:00
abstract：:NK genes are related pan-metazoan homeobox genes. In the fruitfly, NK genes are clustered and involved in patterning various mesodermal derivatives during embryogenesis. It was therefore suggested that the NK cluster emerged in evolution as an ancestral mesodermal patterning cluster. To test this hypothesis, we cloned...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.02.013
更新日期：2008-05-15 00:00:00
abstract：:Using sea urchin embryos, we demonstrate that the MEK/MAPK/ERK cascade is essential for the proper progression of the cell cycle. Activation of a limited fraction of MAPK/ERK is required between S-phase and M-phase. Neither DNA replication nor CDK1 activation are impacted by the inhibition of this small active MAPK/ER...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.11.018
更新日期：2017-01-15 00:00:00
abstract：:This study evaluated Na/K-ATPase alpha 1- and alpha 3-subunit isoform polypeptide expression and localization during bovine preattachment development. Na/K-ATPase cation transport activity from the one-cell to blastocyst stage was also determined by measuring ouabain-sensitive 86Rb+ uptake. Both alpha1- and alpha 3-su...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8874
更新日期：1998-05-01 00:00:00
abstract：:We have isolated Xbr-1, a novel Xenopus homeobox gene that defines a new class of homeobox genes, distantly related to the Drosophila BarH1 and BarH2 genes. Xbr-1 is predominantly expressed in the developing Xenopus eye, starting as early as the neural plate stage. At early stages of eye development XBR-1 is expressed...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0055
更新日期：1996-02-25 00:00:00
abstract：:The homebox gene Hlxb9, encoding Hb9, exhibits a dual expression profile during pancreatic development. The early expression in the dorsal and ventral pancreatic epithelium is transient and spans from embryonic day (e) 8 to e9-e10, whereas the later expression is confined to differentiating beta-cells as they appear. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0440
更新日期：2001-12-01 00:00:00
abstract：:Previous experiments in which vitamin A has been administered to developing or regenerating limbs have shown that different limb axes are affected. In regenerating axolotl limbs, serial reduplications in the proximodistal axis are produced. In the developing chick limb bud, mirror-imaged reduplications in the anteropo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90370-6
更新日期：1983-08-01 00:00:00
abstract：:Epidermal growth factor (EGF) expression and branching morphogenesis were inhibited using a 5' 15-mer antisense oligodeoxynucleotide (ODN) directed against EGF precursor mRNA in embryonic mouse lung in culture under chemically defined, serumless conditions. Antisense EGF ODN resulted in > 90% inhibition of EGF immunor...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1213
更新日期：1993-08-01 00:00:00
abstract：:Cell surface and cytoplasmic polarity is exhibited by the blastomeres of mouse preimplantation embryos following compaction at the 8-cell stage of cleavage. It has been hypothesized that cytoplasmic polarity is initiated by plasma membrane functions of polar blastomeres that are absent from apolar blastomeres. To test...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90433-2
更新日期：1988-11-01 00:00:00
abstract：:Spermatogonial stem cells are required for the initiation of spermatogenesis and the continuous production of sperm. In addition, they can acquire pluripotency and differentiate into derivatives of the three embryonic germ layers when cultured in the appropriate conditions. Therefore, understanding the signaling pathw...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.12.013
更新日期：2007-04-01 00:00:00
abstract：:The acquisition of fertilizability in coelomic eggs of Xenopus laevis has been shown to be correlated with the physical, biochemical, and ultrastructural alterations of the egg envelope [coelomic envelope (CE)] induced during the passage of eggs through the pars recta portion of the oviduct. However, no direct evidenc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9285
更新日期：1999-06-15 00:00:00
abstract：:Despite 30 years of Hox gene study, we have a remarkably limited knowledge of the downstream target genes that Hox transcription factors regulate to confer regional identity. Here, we have used a microarray approach to identify genes that function downstream of a single vertebrate Hox gene, zebrafish hoxb1a. This gene...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.06.012
更新日期：2007-09-15 00:00:00
abstract：:Vitelline coats (VCs) of Phallusia mammillata were isolated and purified following homogenization of live eggs in order to investigate the molecular basis of sperm-egg recognition. Clean VCs were partly solubilized by sonication in H2O and the soluble fraction (SFVC), derived from the outer surface of VCs, was used fo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90272-5
更新日期：1991-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.03.004
更新日期：2005-05-15 00:00:00
abstract：:Genetic interactions regulating intermediate stages of tubulogenesis in the developing kidney have been difficult to define. A systems biology strategy using microarray was combined with in vitro/ex vivo and genetic approaches to identify pathways regulating specific stages of tubulogenesis. Analysis of the progressio...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.01.014
更新日期：2012-04-01 00:00:00
abstract：:The mammalian kidney collecting ducts are critical for water, electrolyte and acid-base homeostasis and develop as a branched network of tubular structures composed of principal cells intermingled with intercalated cells. The intermingled nature of the different collecting duct cell types has made it challenging to id...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.02.007
更新日期：2017-04-01 00:00:00
abstract：:We have cloned a member of the fork head/HNF-3 family of transcription factors from the nematode Caenorhabditis elegans. Within the predicted DNA binding domain, this gene, called Ce-fkh-1, is 75-78% identical to the Drosophila fork head and rat liver HNF-3 alpha, beta, and gamma genes. Ce-fkh-1 mRNA is highly enriche...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0219
更新日期：1996-09-15 00:00:00
abstract：:The GATA family of transcription factors are known to play multiple critical roles in vertebrate developmental processes, including erythropoiesis, endoderm formation and cardiogenesis. There have been no previous demonstrations of a functional role for any GATA family member being associated with musculoskeletal deve...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.12.001
更新日期：2008-02-15 00:00:00
abstract：:Tissue specificity of cell adhesion was directly characterized in a unit cell interaction using a novel laser trapping cell manipulator in combination with a fixed micropipet. We quantified the adhesive specificity of endodermal and ectodermal epithelial cells from Hydra, which are known to sort out within hours after...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1068
更新日期：1994-03-01 00:00:00
abstract：:The lateral line system displays highly divergent patterns in adult teleost fish. The mechanisms underlying this variability are poorly understood. Here, we demonstrate that the lateral line mechanoreceptor, the neuromast, gives rise to a series of accessory neuromasts by a serial budding process during postembryonic ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.02.017
更新日期：2010-04-15 00:00:00
abstract：:We have cloned a partial cDNA of chicken glial cell line-derived neurotrophic factor (GDNF) and systematically examined its expression pattern as well as that of GDNF-binding components (GDNF family receptor alpha-1 and 2: GFRalpha-1 and 2) and a common signal transduction receptor (c-ret protooncogene: RET) during ve...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9543
更新日期：2000-01-01 00:00:00
abstract：:Previous studies have shown that cultured Schwann cells secrete platelet-derived growth factor (PDGF) and express PDGF receptors which transmit a mitogenic response particularly when their levels are upregulated by elevation of intracellular cAMP. In this study the expression of PDGF and PDGF alpha- and beta-receptors...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1044
更新日期：1993-02-01 00:00:00
abstract：:During development of Dictyostelium discoideum, the cellular specific activity of beta-glucosidase increases before aggregation, declines to low levels during pseudoplasmodium formation, and increases rapidly during culmination. In addition, two electrophoretically distinct isozymes of beta-glucosidase are present at ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90407-6
更新日期：1987-10-01 00:00:00
abstract：:CD9 is a membrane protein belonging to the tetraspanin family. Despite CD9's broad tissue distribution, the only abnormality observed in CD9-deficient mice was infertility of females, which was responsible for a defect in the sperm-egg fusion process. However, the function of CD9 in sperm-egg fusion is not clear at al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0694
更新日期：2002-07-15 00:00:00
abstract：:Antibodies to nonerythroid alpha spectrin (p 230) were used to study the distribution of this polypeptide in mouse germ cells, zygote, and early embryonic cells. In the primordial germ cells, fetal oocytes, and spermatogonia, spectrin was found predominantly in the form of a narrow condensed subplasmalemmal band, as i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90389-1
更新日期：1986-03-01 00:00:00
abstract：:The left-right body axis is coordinately aligned with the orthogonal dorsoventral and anterioposterior body axes. The developmental mechanisms that regulate axis coordination are unknown. Here it is shown that the cardiac left-right orientation in zebrafish (Danio rerio) is randomized in notochord-defective no tail an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0148
更新日期：1996-07-10 00:00:00
abstract：:The spe-9 gene is required for fertility in Caenorhabditis elegans and encodes a sperm transmembrane protein with an extracellular domain (ECD) that contains 10 epidermal growth factor (EGF) repeats. Deletion analysis reveals that the EGF repeats and the transmembrane domain are required for fertilization. In contrast...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.05.014
更新日期：2004-08-15 00:00:00
abstract：:The subventricular zone (SVZ) of the developing mammalian forebrain gives rise to astrocytes and oligodendrocytes in the neocortex and white matter, and neurons in the olfactory bulb in perinatal life. We have examined the developmental fates and spatial distributions of the descendants of single SVZ cells by infectin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.02.024
更新日期：2004-06-01 00:00:00
abstract：:The shoot meristem generates all of the aerial structures of an adult plant. It is organized in three layers which produce the epidermis (L1 layer) and the subepidermal layers (L2 and L3). The origin of adult structures has previously been fate mapped to the primary meristem for L2 and L3 tissues. In this work we cons...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0112
更新日期：1996-05-01 00:00:00
