Abstract：
:Despite 30 years of Hox gene study, we have a remarkably limited knowledge of the downstream target genes that Hox transcription factors regulate to confer regional identity. Here, we have used a microarray approach to identify genes that function downstream of a single vertebrate Hox gene, zebrafish hoxb1a. This gene plays a critical and conserved role in vertebrate hindbrain development, conferring identity to hindbrain rhombomere (r) 4. For example, zebrafish Hoxb1a, similar to mouse Hoxb1, is required for the migration of r4-derived facial branchiomotor neurons into the posterior hindbrain. We have screened microarrays carrying more than 16,000 expressed sequence tags (ESTs) for genes that are differentially regulated in normal versus Hoxb1a-deficient r4 tissue. Using this approach, we have identified both positively and negatively regulated candidate Hoxb1a target genes. We have used in situ hybridization to validate twelve positively regulated Hoxb1a targets. These downstream targets are expressed in a variety of subdomains within r4, with one gene, a novel prickle homolog (pk1b), expressed specifically within the facial branchiomotor neurons. Using morpholino knock-down and cell transplantation, we demonstrate that the Hoxb1a target Prickle1b functions cell-autonomously to control facial neuron migration, a single aspect of r4 identity.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Rohrschneider MR,Elsen GE,Prince VEdoi
10.1016/j.ydbio.2007.06.012subject
Has Abstractpub_date
2007-09-15 00:00:00pages
358-72issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(07)01128-1journal_volume
309pub_type
杂志文章abstract：:Growth factors and the extracellular matrix have been shown to fulfill vital developmental roles in many embryonic systems. Our hypothesis is that a developmental role played by the extracellular matrix in sea urchins may be the binding of a PDGF-like growth factor to promote signaling activity. We report here that an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.8059
更新日期：1995-12-01 00:00:00
abstract：:Biomineralization, the biologically controlled formation of mineral deposits, is of widespread importance in biology, medicine, and engineering. Mineralized structures are found in most metazoan phyla and often have supportive, protective, or feeding functions. Among deuterostomes, only echinoderms and vertebrates pro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.07.047
更新日期：2006-12-01 00:00:00
abstract：:DSL (Delta, Serrate, Lag-2) ligands activate Notch signaling and thereby regulate the differentiation of many different cell types during development. We have isolated a novel Serrate-like gene, Jagged2, whose amino acid sequence and expression pattern during rat embryogenesis suggest that it functions as a ligand for...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0310
更新日期：1996-11-25 00:00:00
abstract：:Named for its assembly near exon-exon junctions during pre-mRNA splicing, the exon junction complex (EJC) regulates multiple aspects of RNA biochemistry, including export of spliced mRNAs from the nucleus and translation. Transcriptome analyses have revealed broad EJC occupancy of spliced metazoan transcripts, yet inh...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.09.010
更新日期：2020-01-01 00:00:00
abstract：:The evolution of canalized traits is a central question in evolutionary biology. Natural variation in highly conserved traits can provide clues about their evolutionary potential. Here we investigate natural variation in a conserved trait-even-skipped (eve) expression at the cellular blastoderm stage of embryonic deve...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.06.019
更新日期：2015-09-01 00:00:00
abstract：:Rex1 (Zfp42), GeneID 132625, is a gene whose expression is closely associated with pluripotency/multipotency in both mouse and human embryonic stem cells. To study the function of the murine Rex1 gene in vivo, we have used cre/lox technology to create Rex1(floxed) mice and mice deficient in Rex1 gene function. Rex1(-/...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.05.664
更新日期：2011-08-15 00:00:00
abstract：:The smaller AB-cell of the 2-cell embryo of Tubifex divides 40 min later than the larger CD-cell. The present study was undertaken to elucidate those factors that are responsible for this cleavage asynchrony. An examination of chromosome cycles showed that the AB-cell entered M-phase 5 min later and spent about 35 min...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1123
更新日期：1993-05-01 00:00:00
abstract：:In vertebrates, nodal-related genes are crucial for specifying mesendodermal cell fates. Six nodal-related genes have been identified in Xenopus, but only one, nodal, has been identified in the mouse. The Xenopus nodal-related gene 3 (Xnr3), however, lacks the mesoderm-inducing activity of the other five nodal-related...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.09.015
更新日期：2004-01-01 00:00:00
abstract：:Neurons of the dorsal hindbrain and spinal cord are central in receiving, processing and relaying sensory perception and participate in the coordination of sensory-motor output. Numerous cellular and molecular mechanisms that underlie neuronal development in both regions of the nervous system are shared. We discuss he...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2016.10.008
更新日期：2017-12-01 00:00:00
abstract：:The zebrafish fin is an excellent system to study the mechanisms of dermal bone patterning. Fin rays are segmented structures that form successive bifurcations both during ontogenesis and regeneration. Previous studies showed that sonic hedgehog (shha) may regulate regenerative bone patterning based on its expression ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.03.008
更新日期：2012-05-15 00:00:00
abstract：:We have characterized a phosphoprotein protein with a death effector domain that has a novel bifunctional role in programmed cell death. The 15-kDa phosphoprotein enriched in astrocytes (PEA-15) inhibits Fas-mediated apoptosis and increases tumor necrosis factor receptor-1 (TNF-R1)-mediated apoptosis in the same cell ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9510
更新日期：1999-12-01 00:00:00
abstract：:One of the earliest steps in embryonic development is the establishment of the future body axes. Morphological and molecular data place the Ambulacraria (echinoderms and hemichordates) within the Deuterostomia and as the sister taxon to chordates. Extensive work over the last decades in echinoid (sea urchins) echinode...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.03.030
更新日期：2011-06-01 00:00:00
abstract：:The cellular mechanisms underlying the amazing ability of sea cucumbers to regenerate their autotomized intestines have been widely described by us and others. However, the signaling pathways that control these mechanisms are unknown. Previous studies have shown that Wnt homologs are upregulated during early intestina...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.10.005
更新日期：2020-02-01 00:00:00
abstract：:Mutations that inactivate either merlin (mer) or expanded (ex) result in increased cell growth and proliferation in Drosophila. Both Mer and Ex are members of the Band 4.1 protein superfamily, and, based on analyses of mer ex double mutants, they are proposed to function together in at least a partially redundant mann...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.12.021
更新日期：2007-04-01 00:00:00
abstract：:Vulval epithelial tubes invaginate through concerted cell migration, ring formation, stacking of rings and intra-ring cell fusion in the nematodes Caenorhabditis elegans, Oscheius tipulae and Pristionchus pacificus. The number of rings forming the invaginations is invariantly seven, six, and eight, respectively. We hy...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.10.010
更新日期：2008-01-01 00:00:00
abstract：:We have examined the morphology of fetal rat sympathetic neurons grown in serum-free medium in the absence of nonneuronal cells. Because cell density can affect phenotypic expression in vitro, the morphological analysis was subdivided into the study of isolated neurons (neurons whose somata were at least 150 micron fr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90295-3
更新日期：1988-08-01 00:00:00
abstract：:Pluripotent stem cells, termed embryonic germ (EG) cells, have been generated from both human and mouse primordial germ cells (PGCs). Like embryonic stem (ES) cells, EG cells have the potential to differentiate into all germ layer derivatives and may also be important for any future clinical applications. The developm...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.11.007
更新日期：2008-01-15 00:00:00
abstract：:The Endo16 gene codes for an RGD-containing calcium-binding protein that is found on the basal surfaces and in the extracellular matrix of cells of the invaginating archenteron during sea urchin gastrulation. Previously, we have shown that Endo16 is a single copy gene and we have determined the coding sequence and ana...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0247
更新日期：1996-10-10 00:00:00
abstract：:How species-specific developmental timing is controlled is largely unknown. By following human embryonic stem (ES) cell and mouse epiblast stem (EpiS) cell differentiation through detailed RNA-sequencing time courses, here we show that pluripotent stem cells closely retain in vivo species-specific developmental timing...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.02.002
更新日期：2017-03-15 00:00:00
abstract：:Hedgehog (Hh) proteins are secreted molecules that play an essential role in development and tumorigenesis. In Drosophila cultured cells, phosphorylation of the kinesin-like Costal2 (Cos2) protein at Ser572 is triggered by the kinase fused (Fu) upon Hh pathway activation. Here, we validate the first phospho-antibody f...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.04.021
更新日期：2010-08-01 00:00:00
abstract：:During the process of gastrulation in the sea urchin embryo, the vegetal plate invaginates to form the archenteron, a long narrow tube that extends across the blastocoel. Rearrangement of cells within the archenteron is thought to be a key component of the process of archenteron elongation. While these cell rearrangem...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1154
更新日期：1994-06-01 00:00:00
abstract：:Embryonic morphogenesis relies on the intrinsic ability of cells, often through remodeling the cytoskeleton, to shape epithelial tissues during development. Epithelial invagination is an example of morphogenesis that depends on this remodeling but the cellular mechanisms driving arrangement of cytoskeletal elements ne...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.02.014
更新日期：2020-06-01 00:00:00
abstract：:Using sea urchin embryos, we demonstrate that the MEK/MAPK/ERK cascade is essential for the proper progression of the cell cycle. Activation of a limited fraction of MAPK/ERK is required between S-phase and M-phase. Neither DNA replication nor CDK1 activation are impacted by the inhibition of this small active MAPK/ER...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.11.018
更新日期：2017-01-15 00:00:00
abstract：:Mammalian eggs remain arrested at metaphase of the second meiotic division (metII) for an indeterminate time before fertilization. During this period, which can last several hours, the continued attachment of sister chromatids is thought to be achieved by inhibition of the protease separase. Separase is known to be in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.06.036
更新日期：2008-09-15 00:00:00
abstract：:Caenorhabditis elegans embryonic elongation depends on both epidermal and muscle cells. The hemidesmosome-like junctions, commonly called fibrous organelles (FOs), that attach the epidermis to the extracellular matrix ensure muscle anchoring to the cuticular exoskeleton and play an essential role during elongation. To...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.11.025
更新日期：2011-02-15 00:00:00
abstract：:The Hedgehog (Hh) signal transduction pathway plays a central role in the development of invertebrates and vertebrates. While much is known about the pathway, the role of Suppressor of fused (Su(fu)), a component of the pathway's signaling complex has remained enigmatic. Previous studies have linked Su(fu) to the cyto...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.02.050
更新日期：2006-06-01 00:00:00
abstract：:Primitive erythropoiesis is regulated in a non cell-autonomous fashion across evolution from frogs to mammals. In Xenopus laevis, signals from the overlying ectoderm are required to induce the mesoderm to adopt an erythroid fate. Previous studies in our lab identified the transcription factor GATA2 as a key regulator ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.08.012
更新日期：2015-11-01 00:00:00
abstract：:The embryonic dorsal vessel in Drosophila possesses anteroposterior polarity and is subdivided into two chamber-like portions, the aorta in the anterior and the heart in the posterior. The heart portion features a wider bore as compared with the aorta and develops inflow valves (ostia) that allow the pumping of hemoly...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0839
更新日期：2002-11-15 00:00:00
abstract：:The t haplotypes are variant forms of the proximal portion of chromosome 17 in the mouse. The t haplotypes alter spermatogenesis and many also contain lethal factors. Although the lethal factors vary between t haplotypes, all t haplotypes have the same effect on sperm, that of altering sperm function in fertilization....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1002
更新日期：1993-01-01 00:00:00
abstract：:Intestinal tract development is a coordinated process involving signaling among the progenitors and developing cells from all three germ layers. Development of endoderm-derived intestinal epithelium has been shown to depend on epigenetic modifications, but whether that is also the case for intestinal tract cell types ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.08.002
更新日期：2019-11-15 00:00:00
