Abstract:
:Named for its assembly near exon-exon junctions during pre-mRNA splicing, the exon junction complex (EJC) regulates multiple aspects of RNA biochemistry, including export of spliced mRNAs from the nucleus and translation. Transcriptome analyses have revealed broad EJC occupancy of spliced metazoan transcripts, yet inhibition of core subunits has been linked to surprisingly specific phenotypes and a growing number of studies support gene-specific regulatory roles. Here we report results from a classroom-based RNAi screen revealing the EJC is necessary for regeneration in the planarian flatworm Schmidtea mediterranea. RNAi animals rapidly lost the stem and progenitor cells that drive formation of new tissue during both regeneration and cell turnover, but exhibited normal amputation-induced changes in gene expression in differentiated tissues. Together with previous reports that partial loss of EJC function causes stem cell defects in Drosophila and mice, our observations implicate the EJC as a conserved, posttranscriptional regulator of gene expression in stem cell lineages. This work also highlights the combined educational and scientific impacts of discovery-based research in the undergraduate biology curriculum.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Kimball C,Powers K,Dustin J,Poirier V,Pellettieri Jdoi
10.1016/j.ydbio.2019.09.010subject
Has Abstractpub_date
2020-01-01 00:00:00pages
119-127issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(19)30029-6journal_volume
457pub_type
杂志文章abstract::We have investigated the ability of growing dictyate oocytes and early preimplantation embryos of the mouse to process extrachromosomal DNA molecules with free ends by intranuclearly microinjecting DNA fragments containing a region of homology of various extent at either the 5' or 3' terminus. Homologous recombination...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0199
更新日期:2001-05-01 00:00:00
abstract::The ubiquitin-proteasome system is essential for intracellular protein degradation, but there are few studies of this system in the extracellular milieu. Recently, we reported that a 70-kDa sperm receptor, HrVC70, on the vitelline coat is ubiquitinated and then degraded by the sperm proteasome during fertilization of ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.07.016
更新日期:2003-12-01 00:00:00
abstract::In a previous study, we demonstrated trophic effects of vitamin A and its active metabolite, retinoic acid (RA), on perinatal rat spinal cord neurons and astrocytes in vitro. We now report that RA increases the survival of cholinergic neurons without affecting that of GABAergic neurons. These results were supported by...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90435-6
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abstract::We investigated whether the inhibition of proteoglycan synthesis and salivary branching morphogenesis by beta-D-xyloside was related to the deposition and processing of newly synthesized glycosaminoglycans at the basal epithelial surface that correlates with normal branching activity. Forty eight-hour cultures of cont...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90358-6
更新日期:1985-05-01 00:00:00
abstract::An important developmental question concerns whether neurotransmitter phenotype is an inherent property of neurons or is influenced by target tissues. This issue can be addressed in the avian ciliary ganglion (CG) which contains two cholinergic populations, ciliary and choroid neurons, that differentially express the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8998
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abstract::We have identified cDNA clones encoding a testis-specific poly(A) polymerase, termed TPAP, a candidate molecule responsible for cytoplasmic polyadenylation of preexisting mRNAs in male haploid germ cells. The TPAP gene was most abundantly expressed coincident with the additional elongation of mRNA poly(A) tails in rou...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9894
更新日期:2000-12-01 00:00:00
abstract::In Caenorhabditis elegans, Wnt signaling regulates many asymmetric cell divisions. During embryogenesis, the C. elegans Dishevelled (Dsh) homolog, DSH-2, regulates asymmetric neuroblast division of the ABpl/rpppa blast cell. Dsh is a key intracellular component of both beta-catenin dependent and beta-catenin independe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.01.025
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abstract::Hox genes are thought to participate in patterning the anteroposterior (a-p) axis during vertebrate embryogenesis. In this investigation, the spatial expression of six Hoxb genes was analyzed in early embryos of Xenopus laevis by in situ hybridization. Hoxb gene expression was first detected in late gastrulae/early ne...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1330
更新日期:1994-12-01 00:00:00
abstract::When a posterior fragment of the chick's marginal zone (PM) was exchanged with equal sized lateral marginal zone fragment (LM), of the same blastoderm, its capacity to initiate an ectopic primitive streak (PS) was found to be both size and stage dependent. Good correlation was demonstrated between the areas of PM frag...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90091-2
更新日期:1989-07-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.07.013
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1287
更新日期:1993-11-01 00:00:00
abstract::Peptide growth factors can initiate changes in cell fate in Xenopus ectodermal explants and induce the formation of mesoderm. Marker genes expressed in mesoderm allow the analysis of whether, or how much, induction has occurred, but do not tell us what molecules are involved in carrying out the response. In this repor...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0158
更新日期:1996-07-10 00:00:00
abstract::Fertilization triggers activation of Src-family kinases in eggs of various species including marine invertebrates and lower vertebrates. While immunofluorescence studies have localized Src-family kinases to the plasma membrane or cortical cytoplasm, no information is available regarding the extent to which these kinas...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.03.041
更新日期:2006-07-15 00:00:00
abstract::The cellular mechanisms underlying the amazing ability of sea cucumbers to regenerate their autotomized intestines have been widely described by us and others. However, the signaling pathways that control these mechanisms are unknown. Previous studies have shown that Wnt homologs are upregulated during early intestina...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.10.005
更新日期:2020-02-01 00:00:00
abstract::Copyright ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8954
更新日期:1998-07-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90039-8
更新日期:1983-02-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8823
更新日期:1998-03-01 00:00:00
abstract::Syndecan-3 is one of four identified members of a family of transmembrane proteoglycans (the syndecans) that possess highly similar cytoplasmic and transmembrane domains and may function as extracellular matrix receptors and/or low affinity receptors for signaling molecules such as FGF. We previously reported the clon...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1093
更新日期:1995-04-01 00:00:00
abstract::Hand proteins are evolutionally conserved basic helix-loop-helix (bHLH) transcription factors implicated in development of neural crest-derived tissues, heart and limb. Hand1 is expressed in the distal (ventral) zone of the branchial arches, whereas the Hand2 expression domain extends ventrolaterally to occupy two-thi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.07.036
更新日期:2007-10-01 00:00:00
abstract::Polycomb group (PcG) proteins are best known for their role in maintaining stable, mitotically heritable silencing of the homeotic (HOX) genes during development. In addition to loss of homeotic gene silencing, some PcG mutants also have small imaginal discs. These include mutations in E(z), Su(z)12, esc and escl, whi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.03.011
更新日期:2013-08-01 00:00:00
abstract::Mesenchyme to epithelium transitions are crucial to embryonic development. The early mouse embryo offers an excellent model to study epithelium formation as during the first three days of development two epithelia are formed, the trophectoderm (TE) and the primitive endoderm (PrE). We have previously shown that PrE ce...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.10.048
更新日期:2008-01-15 00:00:00
abstract::We previously demonstrated that male mice deficient in the soluble adenylyl cyclase (sAC) are sterile and produce spermatozoa with deficits in progressive motility and are unable to fertilize zona-intact eggs. Here, analyses of sAC(-/-) spermatozoa provide additional insights into the functions linked to cAMP signalin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.05.038
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abstract::ragged seedling2 (rgd2) is a novel, recessive mutation affecting lateral organ development in maize. The mutant phenotype of homozygous rgd2-R leaves is variable. Mild leaf phenotypes have a reduced midrib and may be moderately narrow and furcated; severe Rgd2-R(-) leaves are filamentous or even radial. Despite their ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.03.027
更新日期:2005-06-15 00:00:00
abstract::A chronic neuromuscular blockade during those embryonic stages when naturally occurring spinal motoneuron death occurs, results in the prevention of this cell loss. The excess motoneurons are maintained as long as the neuromuscular blockade is continued; once embryonic neuromuscular activity resumes, however, the exce...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90114-3
更新日期:1984-01-01 00:00:00
abstract::Fibroblast growth factor (FGF) signalling has been implicated in the generation of mesoderm and neural fates in chordate embryos including ascidians and vertebrates. In Ciona, FGF9/16/20 has been implicated in both of these processes. However, in FGF9/16/20 knockdown embryos, notochord fate recovers during later devel...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.08.075
更新日期:2007-02-01 00:00:00
abstract::To examine the relationship between growth hormone (GH) and insulin-like growth factor 1 (IGF1) in controlling postnatal growth, we performed a comparative analysis of dwarfing phenotypes manifested in mouse mutants lacking GH receptor, IGF1, or both. This genetic study has provided conclusive evidence demonstrating t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9975
更新日期:2001-01-01 00:00:00
abstract::Plants often display a high competence for regeneration under stress conditions. Signals produced in response to various types of stress serve as critical triggers for de novo organogenesis, but the identity of these signaling molecules underlying cellular reprogramming are largely unknown. We previously identified an...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.07.006
更新日期:2018-10-01 00:00:00
abstract::A gene regulatory network subcircuit comprising the otx, wnt8, and blimp1 genes accounts for a moving torus of gene expression that sweeps concentrically across the vegetal domain of the sea urchin embryo. Here we confirm by mutation the inputs into the blimp1cis-regulatory module predicted by network analysis. Its es...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.10.042
更新日期:2008-01-15 00:00:00
abstract::We utilized a strain of mice, derived from a radiation mutagenesis experiment and carrying an activity-attenuated allele of the X-linked enzyme glucose-6-phosphate dehydrogenase (G6PD), to analyze the development of the cell lineage leading to cerebellar Purkinje neurons. Due to random X inactivation during early embr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0083
更新日期:1996-03-15 00:00:00
abstract::The dopaminergic neurons of the basal ganglia play critical roles in CNS function and human disease, but specification of dopamine neuron phenotype is poorly understood in vertebrates. We performed an in vivo screen in zebrafish to identify dopaminergic neuron enhancers, in order to facilitate studies on the specifica...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.01.023
更新日期:2011-04-15 00:00:00