Abstract：
:We have studied the effect of saline and acid extracts of chick brain on the total number of acetylcholine (ACh) receptors and the number of receptor clusters in cultured chick muscle cells. Myotubes in 7-day cultures responded more rapidly to brain extract than did myotubes in 4-day cultures, so the older cells were used in subsequent bioassays. A large percentage of the receptor inducing activity was soluble in 2% trifluoroacetic acid (TFA), and this material appeared by Sephadex G-25 chromatography to be about 1000 daltons in size. Activity was retained on octadecasilyl silica and was further purified by reverse-phase high-pressure liquid chromatography using a TFA-acetonitrile gradient system. Material that eluted between 35 and 40% acetonitrile, termed C4018, was 500- to 1000-fold more potent than saline extract. The receptor accumulation induced by C4018 was associated with an increased rate of receptor incorporation, presumably receptor synthesis, rather than to a decrease in receptor degradation. An increase in incorporation was detected as early as 3 hr after C4018 was added to 7-day cultures and the effect was maximal after 10 hr. C4018 also promoted the aggregation of receptors that were already incorporated in the surface membrane at the time to addition. It is not yet known if aggregation of "old" receptors and increased receptor synthesis are related or if the two phenomena are mediated by the same molecule.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Buc-Caron MH,Nystrom P,Fischbach GDdoi
10.1016/0012-1606(83)90039-8subject
Has Abstractpub_date
1983-02-01 00:00:00pages
378-86issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(83)90039-8journal_volume
95pub_type
杂志文章abstract：:Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations. ...
journal_title：Developmental biology
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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journal_title：Developmental biology
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journal_title：Developmental biology
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