Abstract:
:Polycomb group (PcG) proteins are best known for their role in maintaining stable, mitotically heritable silencing of the homeotic (HOX) genes during development. In addition to loss of homeotic gene silencing, some PcG mutants also have small imaginal discs. These include mutations in E(z), Su(z)12, esc and escl, which encode Polycomb repressive complex 2 (PRC2) subunits. The cause of this phenotype is not known, but the human homologs of PRC2 subunits have been shown to play a role in cell proliferation, are over-expressed in many tumors, and appear to be required for tumor proliferation. Here we show that the small imaginal disc phenotype arises, at least in part, from a cell growth defect. In homozygous E(z) mutants, imaginal disc cells are smaller than cells in normally proliferating discs. We show that the Thor gene, which encodes eIF4E-binding protein (4E-BP), the evolutionarily conserved inhibitor of cap-dependent translation and potent inhibitor of cell growth, is involved in the development of this phenotype. The Thor promoter region contains DNA binding motifs for transcription factors found in well-characterized Polycomb response elements (PREs), including PHO/PHOL, GAGA factor, and others, suggesting that Thor may be a direct target of Polycomb silencing. We present chromatin immunoprecipitation evidence that PcG proteins are bound to the Thor 5' region in vivo. The Thor gene is normally repressed in imaginal discs, but Thor mRNA and 4E-BP protein levels are elevated in imaginal discs of PRC2 subunit mutant larvae. Deletion of the Thor gene in E(z) mutants partially restores imaginal disc size toward wild-type and results in an increase in the fraction of larvae that pupariate. These results thus suggest that PcG proteins can directly modulate cell growth in Drosophila, in part by regulating Thor expression.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Mason-Suares H,Tie F,Yan CM,Harte PJdoi
10.1016/j.ydbio.2013.03.011subject
Has Abstractpub_date
2013-08-01 00:00:00pages
111-24issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(13)00140-1journal_volume
380pub_type
杂志文章abstract::GDF11, a new member of the TGF-beta gene superfamily, regulates anterior/posterior patterning in the axial skeleton during mouse embryogenesis. Gdf11 null mice display skeletal abnormalities that appear to represent anterior homeotic transformations of vertebrae consistent with high levels of Gdf11 expression in...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9981
更新日期:2001-01-15 00:00:00
abstract::Drosophila Polycomb Repressive Complex 2 (PRC2) is a lysine methyltransferase that trimethylates histone H3 lysine 27 (H3K27me3), a modification essential for Polycomb silencing. Mutations in its catalytic subunit, E(Z), that abolish its methyltransferase activity disrupt Polycomb silencing, causing derepression of Po...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.12.007
更新日期:2012-04-15 00:00:00
abstract::Runx2-Cbfa1, a Runt transcription factor, plays important roles during skeletal development. It is required for differentiation and function of osteoblasts. In its absence, chondrocyte hypertrophy is severely impaired and there is no vascularization of cartilage templates during skeletal development. These tissue-spec...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.03.003
更新日期:2004-06-15 00:00:00
abstract::Ephrins and semaphorins regulate a wide variety of developmental processes, including axon guidance and cell migration. We have studied the roles of the ephrin EFN-4 and the semaphorin MAB-20 in patterning cell-cell contacts among the cells that give rise to the ray sensory organs of Caenorhabditis elegans. In wild-ty...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(02)00129-x
更新日期:2003-04-15 00:00:00
abstract::The mouse protamine mRNAs, Prm-1 and Prm-2, are translationally repressed for several days during male germ cell differentiation. The translational delay of mouse Prm-1 mRNA has previously been shown to be dependent upon cis-acting elements that reside in the last 62 nucleotides of the Prm-1 3' untranslated region (3'...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8705
更新日期:1997-11-01 00:00:00
abstract::Completion of cytokinesis, abscission, has been studied little despite the intensive studies of the onset and contractile mechanism of the earlier phases of division. It has been well documented that microtubule (MT) disruption before furrow stimulation prevents furrowing, while MT disruption after furrow stimulation ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9395
更新日期:1999-10-01 00:00:00
abstract::About 12-17% of the embryos obtained by mating mice carrying the In(X)1H or Paf mutations are of the 39,X (X0) genotype. Depending on the mutant mice used for mating, the monosomic X chromosome can be inherited from the paternal (XP) or the maternal (XM) parent. The XP0 embryos display developmental retardation at gas...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8972
更新日期:1998-09-01 00:00:00
abstract::A major cause for vision impairment and blindness in industrialized countries is the loss of the light-sensing retinal tissue in the eye. Photoreceptor damage is one of the main characteristics found in retinal degeneration diseases, such as Retinitis Pigmentosa or age-related macular degeneration. The lack of effecti...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2017.09.028
更新日期:2018-01-15 00:00:00
abstract::Despite 30 years of Hox gene study, we have a remarkably limited knowledge of the downstream target genes that Hox transcription factors regulate to confer regional identity. Here, we have used a microarray approach to identify genes that function downstream of a single vertebrate Hox gene, zebrafish hoxb1a. This gene...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.06.012
更新日期:2007-09-15 00:00:00
abstract::Hair shafts are produced from stem cells located in the bulge. Our knowledge of the genetic pathways regulating cell fate acquisition in the immediate descendents of these stem cells, and fate maintenance in their committed progeny, is still incomplete. One pathway involved in fate maintenance within the hair matrix i...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.11.021
更新日期:2009-02-15 00:00:00
abstract::We examined the development of epithelial Na+ conductance in 6- and 7-day post coitus (p.c.) preimplantation rabbit embryos using the whole-cell patch-clamp technique on dissociated rabbit trophectodermal cells and by immunocytochemical localization using a polyclonal antibody directed against subunits of an apical ep...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90289-f
更新日期:1991-10-01 00:00:00
abstract::An anterior-posterior concentration difference of fibronectin associated with the endoderm in early chick embryos has been implicated in the directional migration of precardiac mesoderm cells. We have examined the effect of increasing concentrations of an antibody to fibronectin (FN) to test the essentiality of FN to ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90378-8
更新日期:1988-10-01 00:00:00
abstract::Epigenetic gene control is involved in mechanisms of development. Little is known about the cooperation of nuclear and chromatin events in programmed differentiation from mouse embryonic stem cells (ESC). To address this, Oct3/4-positive ESC and differentiated progenies, Sox1-positive neural precursor cells (NPC) and ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.04.450
更新日期:2006-10-15 00:00:00
abstract::Proper patterning and growth of oral structures including teeth, tongue, and palate rely on epithelial-mesenchymal interactions involving coordinated regulation of signal transduction. Understanding molecular mechanisms underpinning oral-facial development will provide novel insights into the etiology of common congen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.05.002
更新日期:2011-08-01 00:00:00
abstract::The changes in the intracellular pH (pHi) of sea urchin sperm associated with motility initiation and acrosome reaction were investigated using uptake of two different probes; 9-aminoacridine and methylamine, as a qualitative index. Sperm suspended in Na+-free sea water were immotile and able to concentrate these amin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90004-0
更新日期:1983-01-01 00:00:00
abstract::Crest-derived glomus cells of the carotid body (CB) are O(2)-sensitive chemoreceptors, which resemble sympathoadrenal (SA) chromaffin cells. In this study, we tested whether perinatal rat glomus cells are sensitive to basic fibroblast growth factor (bFGF) in vitro and whether their sensitivity is regulated by oxygen. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8539
更新日期:1997-04-15 00:00:00
abstract::In this report we describe the development of a sensitive assay for gene expression in zebrafish embryos using beta-lactamase as a reporter gene. We show that injection of a green fluorescent substrate for beta-lactamase allows the detection of reporter gene expression in live embryos. The beta-lactamase enzyme cataly...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8999
更新日期:1998-11-15 00:00:00
abstract::Using an in vitro model of rat epiphyseal chondrocyte differentiation in which cells are maintained in a three-dimensional cell pellet, we show that exogenous TGF-beta 1 reversibly prevents terminal differentiation of epiphyseal chondrocytes into hypertrophic cells. Through maintenance of gene expression for the carti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1200
更新日期:1993-08-01 00:00:00
abstract::There is a natural curiosity about how organisms give rise to offspring like themselves through a series of reproducible developmental events and how, once mature, these offspring mate and continue the process giving rise the next generation. In the mid-1800s investigators started using gametes and embryos to explore ...
journal_title:Developmental biology
pub_type: 历史文章,杂志文章
doi:10.1016/j.ydbio.2011.06.021
更新日期:2011-10-15 00:00:00
abstract::The expression of heat shock genes is induced in all living cells by a series of proteotoxic treatments. Heat shock genes are also activated spontaneously during different phases of embryonic development. HSP89 alpha and HSC70 are expressed at a high level in the mouse blastocyst. A family of factors, called HSFs, are...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1361
更新日期:1994-12-01 00:00:00
abstract::Proper development of a multicellular organism relies on well-coordinated regulation of cell fate specification, cell proliferation and cell differentiation. The C. elegans postembryonic mesoderm provides a useful system for uncovering factors involved in these processes and for further dissecting their regulatory rel...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.06.011
更新日期:2017-09-01 00:00:00
abstract::Early (alpha) histone genes are one of several histone gene families in the sea urchin genome. They are expressed at high levels in blastula-stage embryos and are inactivated by the early gastrula stage. By microinjecting mutant early H2B genes into sea urchin zygotes and monitoring their transcriptional activity in b...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90248-f
更新日期:1992-04-01 00:00:00
abstract::The vertebrate intestinal epithelium is renewed continuously from stem cells at the base of the crypt in mammals or base of the fold in fish over the life of the organism. As stem cells divide, newly formed epithelial cells make an initial choice between a secretory or enterocyte fate. This choice has previously been ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.01.013
更新日期:2013-04-15 00:00:00
abstract::Complex intrinsic and extrinsic mechanisms determine neural cell fate during development of the nervous system. Using Id4 deficient mice, we show that Id4 is required for normal development of the central nervous system (CNS), timing neural differentiation in the developing forebrain. In the absence of Id4, the ventri...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.02.001
更新日期:2005-04-15 00:00:00
abstract::We have examined the expression of mRNAs encoding five major neurotransmitter-synthesizing enzymes in MAH cells, a clonal cell line derived by retroviral immortalization of a rat embryonic sympathoadrenal progenitor cell. These mRNAs include tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), tryptophan hydro...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90313-r
更新日期:1991-11-01 00:00:00
abstract::Mammalian heart development involves complex morphogenetic events which lead to the formation of fully separated left and right atrial and ventricular chambers from a tubular heart. Separation of left and right ventricular chambers is dependent on a single structure, the interventricular septum (IVS), which has both m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.045
更新日期:2006-06-15 00:00:00
abstract::The HNK-1 antibody recognizes a carbohydrate moiety that is shared by a family of cell adhesion molecules and is also present on the surface of migrating neural crest cells. Here, the effects of the HNK-1 antibody on neural crest cells were examined in vitro and in vivo. When the HNK-1 antibody was added to neural tub...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90390-3
更新日期:1987-10-01 00:00:00
abstract::Unequal first cleavage is characteristic of a diverse group of protostome animals. In the nematode Caenorhabditis elegans, unequal first cleavage is achieved through the interaction of an apparently symmetric mitotic spindle apparatus with a clearly polarized cell cortex. In the clitellate annelid Tubifex tubifex, by ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.12.049
更新日期:2006-04-01 00:00:00
abstract::Hoxa-11 and Hoxd-11 are paralogous genes required for proper development of the vertebral column, the limbs, and the urogenital system. To further explore the functional relationship between these genes, as well as the potential rescue of one function by the other, we have introduced a Hoxd-11-expressing transgene int...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0137
更新日期:1996-06-15 00:00:00
abstract::Similar amounts of expression of a luciferase reporter gene that is driven by the SV40 early promoter are detected in G2 of 1-cell mouse embryo following injection of the male pronucleus in either early or mid/late S. No luciferase activity is observed when the male pronucleus is injected in early S and the embryos an...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1101
更新日期:1993-04-01 00:00:00