Abstract：
:DNA amplification for two major DNA puffs (II/2B and II/9A) of the fungus fly Sciara coprophila increases steeply from 17 to 19 days after hatching (18 degrees C), resulting in almost 20-fold more DNA at these loci in mature larval salivary glands than in adult tissues. At 19 days after hatching when gene amplification reaches a plateau, there is a burst in the amount of mRNA encoded by these two DNA puffs. Expansion of the two puffs coincides with the increase in transcription rather than reinitiation of DNA replication. In contrast, an RNA puff (III/9B) undergoes no DNA amplification, and the burst in RNA produced by this locus occurs slightly later, at 20 days after hatching. The progression of cytological puffing and associated molecular events of DNA amplification and transcription correlate with features of the external phenotype of the larvae, especially the number of pigment granules in the eyespots that are the anlage to the adult eyes. The sequential and coordinated regulation of DNA puff replication and transcription is discussed.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Wu N,Liang C,DiBartolomeis SM,Smith HS,Gerbi SAdoi
10.1006/dbio.1993.1287subject
Has Abstractpub_date
1993-11-01 00:00:00pages
73-84issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(83)71287-Xjournal_volume
160pub_type
杂志文章abstract：:This work describes unknown aspects of chordotonal organ (ChO) morphogenesis revealed in post-embryonic stages through the use of new fluorescently labeled markers. We show that towards the end of embryogenesis a hitherto unnoticed phase of cell migration commences in which the cap cells of the ventral ChOs elongate a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.01.009
更新日期：2016-03-01 00:00:00
abstract：:Glycosaminoglycans in the form of heparan sulfate proteoglycans (HSPG) and chondroitin sulfate proteoglycans (CSPG) are required for normal kidney organogenesis. The specific roles of HSPGs and CSPGs on ureteric bud (UB) branching morphogenesis are unclear, and past reports have obtained differing results. Here we emp...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.04.029
更新日期：2004-08-15 00:00:00
abstract：:The thyroid gland synthesizes thyroxine (T4), which passes through the larval tadpole's circulatory system. The enzyme type II iodothyronine deiodinase (D2) converts thyroxine (T4) to the active hormone 3,5,3'-triiodothyronine (T3) in peripheral tissues. An early response to thyroid hormone (TH) in the Xenopus laevis ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.10.005
更新日期：2004-02-01 00:00:00
abstract：FOREWORD:The neural crest has been the main object of my investigations during my career in science, up to now. It is a fascinating topic for an embryologist because of its two unique characteristics: its large degree of multipotency and the fact that its development involves a phase during which its component cells mi...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.07.019
更新日期：2018-12-01 00:00:00
abstract：:In Xenopus, the hatching and cement glands form at important boundaries in the head. We have examined induction of these glands in order to see the basis for this ectodermal patterning. Ectoderm can be induced to form hatching and cement glands by dorsal mesoderm and by the neural plate. Because the neural plate is su...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1183
更新日期：1993-07-01 00:00:00
abstract：:An understanding of heart development is critical in any systems biology approach to cardiovascular disease. The interpretation of data generated from high-throughput technologies (such as microarray and proteomics) is also essential to this approach. However, characterizing the role of genes in the processes underlyi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.03.011
更新日期：2011-06-01 00:00:00
abstract：:The corepressor SMRT acts on a range of transcription factors, including the retinoid and thyroid hormone nuclear receptors. The carboxy-terminal region of SMRT contains CoRNR box motifs that mediate these interactions. We have shown, in Xenopus, that SMRT can exist as isoforms containing either two or three CoRNR box...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.007
更新日期：2006-04-15 00:00:00
abstract：:Initiation of transcription mediated by RNA polymerase II requires a number of transcription factors among which TFIID is the major core promoter recognition factor. TFIID is composed of highly conserved factors which include the TATA-binding protein (TBP) and about 14 TBP-associated factors (TAFs). Recently, the comp...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.06.006
更新日期：2005-09-01 00:00:00
abstract：:TGF-beta superfamily members signal through a heteromeric receptor complex to regulate craniofacial development. TGF-beta type II receptor appears to bind only TGF-beta, whereas TGF-beta type I receptor (ALK5) also binds to ligands in addition to TGF-beta. Our previous work has shown that conditional inactivation of T...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.03.045
更新日期：2008-08-01 00:00:00
abstract：:Oct-4 is the earliest expressed gene known to encode a transcription factor which is developmentally regulated during mammalian embryogenesis. In order to understand the role of Oct-4 in early murine embryogenesis, we carried out an analysis of the temporal and spatial pattern of protein expression. We report the pres...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1312
更新日期：1994-11-01 00:00:00
abstract：:Sex-determination in Caenorhabditis elegans requires regulation of gene transcription and protein activity and stability. sel-10 encodes a WD40-repeat-containing F-box protein that likely mediates the ubiquitin-mediated degradation of important sex-determination factors. Loss of sel-10 results in a mild masculinizatio...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.07.035
更新日期：2008-10-15 00:00:00
abstract：:Monoclonal antibodies have been generated to determine the fate of sperm-specific surface components of the sea urchin, Arbacia punctulata, subsequent to gamete fusion. Monoclonal antibody-7 (MAB-7) reacted with two polypeptide bands having apparent molecular masses of 33 and 35 kDa derived from the sperm plasma membr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(89)80036-3
更新日期：1989-01-01 00:00:00
abstract：:In vertebrates, nodal-related genes are crucial for specifying mesendodermal cell fates. Six nodal-related genes have been identified in Xenopus, but only one, nodal, has been identified in the mouse. The Xenopus nodal-related gene 3 (Xnr3), however, lacks the mesoderm-inducing activity of the other five nodal-related...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.09.015
更新日期：2004-01-01 00:00:00
abstract：:Elastic fibers are produced during fetal and neonatal periods, and by maturity elastin expression has ceased. Fetal bovine hyaline chondrocytes acquired an elastogenic phenotype within 24 hr after isolation from the tissue, even though the tissue does not produce elastic fibers or tropoelastin mRNA in vivo. By multipl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1140
更新日期：1994-05-01 00:00:00
abstract：:Lineage specification of multipotent progenitor cells is governed by a balance of lineage-affiliated transcription factors, such as GATA1 and PU.1, which regulate the choice between erythroid and myelomonocytic fates. But how ratios of lineage-determining transcription factors stabilize progenitor cells and resolve th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.02.036
更新日期：2007-05-15 00:00:00
abstract：:To investigate the lineage relationships of neurons and astroglial cells early in central nervous system development, we have analyzed the progeny of neural plate cells in an amphibian embryo (Pleurodeles waltl). A fluorescent tracer, lysinated rhodamine-dextran, was iontophoretically injected into individual precurso...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1233
更新日期：1993-09-01 00:00:00
abstract：:Eye cups from stage 14-28 (E2 to E5) chick and quail embryos consisting of neural retina, lens, and vitreous body were cultured for 1 or 2 days. These eyes expanded by proliferation of the retinal cells and the surface areas of the retinae increased several-fold. The area covered by ganglion cells and axons also expan...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90194-6
更新日期：1986-04-01 00:00:00
abstract：:Joint and skeletal development is highly regulated by extracellular matrix (ECM) proteoglycans, of which chondroitin sulfate proteoglycans (CSPGs) are a major class. Despite the requirement of joint CSPGs for skeletal flexibility and structure, relatively little is understood regarding their role in establishing joint...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.01.005
更新日期：2012-03-15 00:00:00
abstract：:CHARGE syndrome is caused by spontaneous loss-of-function mutations to the ATP-dependant chromatin remodeller chromodomain-helicase-DNA-binding protein 7 (CHD7). It is characterised by a distinct pattern of congenital anomalies, including cardiovascular malformations. Disruption to the neural crest lineage has previou...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.06.017
更新日期：2015-09-01 00:00:00
abstract：:The formation of the spinal cord during early embryonic development in vertebrate embryos is a continuous process that begins at gastrulation and continues through to the completion of somitogenesis. Despite the conserved usage of patterning mechanisms and gene regulatory networks that act to generate specific spinal ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2017.01.021
更新日期：2017-12-01 00:00:00
abstract：:Embryonic morphogenesis relies on the intrinsic ability of cells, often through remodeling the cytoskeleton, to shape epithelial tissues during development. Epithelial invagination is an example of morphogenesis that depends on this remodeling but the cellular mechanisms driving arrangement of cytoskeletal elements ne...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.02.014
更新日期：2020-06-01 00:00:00
abstract：:Full-grown Xenopus oocytes, Stage VI (1200-1300 microns), undergo meiotic maturation when exposed to progesterone. Smaller stage IV (800 microns) and stage V (1000 microns) oocytes remain in prophase arrest when exposed to this steroid. The larger stage VI oocytes undergo an intracellular alkalization from 7.2 to 7.6,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90288-4
更新日期：1984-10-01 00:00:00
abstract：:Kinesin light chain 3 (KLC3) is the only known kinesin light chain expressed in post-meiotic male germ cells. We have reported that in rat spermatids KLC3 associates with outer dense fibers and mitochondrial sheath. KLC3 is able to bind to mitochondria in vitro and in vivo employing the conserved tetratrico-peptide re...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.04.026
更新日期：2012-06-15 00:00:00
abstract：:The heart is the first organ to form and function in the vertebrate embryo. Furthermore, differences between the left and right sides of the embryo become first detectable during cardiac development. We observed strong cardiac laterality phenotypes in medaka embryos by manipulating Groucho protein activity. The phenot...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.11.020
更新日期：2007-03-01 00:00:00
abstract：:Intracellular pH (pHi) measurements were performed in surf clam (Spisula solidissima) oocytes before and after artificial activation or fertilization [evidenced by germinal vesicle breakdown (GVBD)] by the dimethyloxazolidinedione (DMO) and 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) methods. Resul...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8682
更新日期：1997-10-01 00:00:00
abstract：:Little is known about the biochemical changes underlying the morphological differentiation of the sea urchin egg during oogenesis. Because of this and the essential role of gamete recognition in fertilization, we studied the developmental expression of the recently identified egg surface receptor for sperm during ooge...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1233
更新日期：1994-09-01 00:00:00
abstract：:A careful reanalysis of the literature indicates that the initial mechanism of activation in sea urchin eggs is remarkably similar to the mechanism established in medaka eggs: i.e., sea urchin eggs are activated by a qualitatively and quantitatively similar calcium explosion; one which is propagated in a wave sustaine...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/0012-1606(83)90276-2
更新日期：1983-10-01 00:00:00
abstract：:The current academic and business landscape does not provide a clear path for doctoral trainees graduating. Moreover, the fundamental need of the hour is diversification of the workforce where PhD graduates need to be trained to do more beyond basic scientific research. One of the possible paths that is now becoming p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.10.025
更新日期：2020-03-01 00:00:00
abstract：:alpha11beta1 integrin constitutes a recent addition to the integrin family. Here, we present the first in vivo analysis of alpha11 protein and mRNA distribution during human embryonic development. alpha11 protein and mRNA were present in various mesenchymal cells around the cartilage anlage in the developing skeleton ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0363
更新日期：2001-09-01 00:00:00
abstract：:Neonatal mouse hearts fully regenerate after ventricular resection similar to adult zebrafish. We established cryoinjury models to determine if different types and varying degrees of severity in cardiac injuries trigger different responses in neonatal mouse hearts. In contrast to ventricular resection, neonatal mouse ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.12.018
更新日期：2015-03-01 00:00:00
