Abstract：
:Oct-4 is the earliest expressed gene known to encode a transcription factor which is developmentally regulated during mammalian embryogenesis. In order to understand the role of Oct-4 in early murine embryogenesis, we carried out an analysis of the temporal and spatial pattern of protein expression. We report the presence of Oct-4 protein in cultured cells and murine embryos as determined by immunohistochemistry using confocal microscopy. Oct-4 protein is present in both embryonic stem cells and embryonal carcinoma cells, but is down-regulated following differentiation of these cells by culture in the absence of leukemia inhibitory factor or in the presence of retinoic acid, respectively. In embryos, the protein is found at low levels in unfertilized eggs and is localized predominantly to the pro-nuclei upon their formation following fertilization. The protein is present in the nuclei in all cleavage stages, but following differentiation of the trophectoderm at the blastocyst stage, Oct-4 protein is only expressed in the inner cell mass. The pattern of protein expression to this stage correlates well with previously reported in situ hybridization results; however, a striking difference was seen in primitive endoderm cells which had begun to differentiate and migrate along the inner surface of the trophectoderm. In direct contrast to RNA localization studies which demonstrate that there are only low levels of Oct-4 transcripts in primitive endoderm cells, protein expression in these early migrating cells is higher than in the inner cell mass.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Palmieri SL,Peter W,Hess H,Schöler HRdoi
10.1006/dbio.1994.1312subject
Has Abstractpub_date
1994-11-01 00:00:00pages
259-67issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(84)71312-1journal_volume
166pub_type
杂志文章abstract：:Gap junctions are intercellular channels made of connexins (Cxs) that allow direct communication between adjacent cells. Modulation of Cxs has been associated with abnormal development and function of the mammary gland and breast cancer. However, the mechanisms underlying their expression during normal mammary gland a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.06.011
更新日期：2016-08-01 00:00:00
abstract：:Specification of the trophectoderm (TE) and inner cell mass (ICM) lineages in the mouse blastocyst correlates with cell position, as TE derives from outer cells whereas ICM from inner cells. Differences in position are reflected by cell polarization and Hippo signaling. Only in outer cells, the apical-basal cell polar...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.06.023
更新日期：2014-10-01 00:00:00
abstract：:Immunocytochemical studies were performed to determine if denervated rodent iris produces nerve growth factor (NGF) in a form chemically similar to that of the 7S NGF complex in mouse submandibular glands. Antisera to the alpha, beta, and gamma subunits of 7S NGF were raised in rabbits and characterized on immunoblots...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90201-0
更新日期：1986-04-01 00:00:00
abstract：:Development of the mammalian external genitalia is controlled by a network of signaling molecules and transcription factors. Because FGF signaling plays a central role in this complicated morphogenetic process, we investigated the role of Sprouty genes, which are important intracellular modulators of FGF signaling, du...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.12.014
更新日期：2014-02-01 00:00:00
abstract：:Male germline stem cells (GSCs) in Drosophila melanogaster divide asymmetrically by orienting the mitotic spindle with respect to the niche, a microenvironment that specifies stem cell identity. The spindle orientation is prepared during interphase through stereotypical positioning of the centrosomes. We recently demo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.10.010
更新日期：2012-01-01 00:00:00
abstract：:Acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF) are present in high levels in most areas of the embryonic rodent brain. To begin to understand the role of these growth factors in brain development, the effects of aFGF and bFGF on dissociated cell cultures prepared from embryonic and ne...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90143-5
更新日期：1992-08-01 00:00:00
abstract：:Melanoblasts, the precursors of the pigment-producing cells of the skin and hair, are derived from the neural crest and migrate to the skin around 12 days of gestation in the mouse. In adult mice almost all the melanoblasts are confined to the hair follicles except for the epidermal layers of nonhairy skin. The recept...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9856
更新日期：2000-09-15 00:00:00
abstract：:We describe the identification, biochemical characterisation, and mutation of a novel mouse gene: Sp5. Sp5 encodes a protein having a C-terminal C(2)H(2) zinc finger domain closely related to that of the transcription factor Sp1. In vitro, DNA binding studies show that it binds to the GC box, a DNA motif present in th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9878
更新日期：2000-11-15 00:00:00
abstract：:Cranial nerves VII, IX and X provide both gustatory (taste) and non-gustatory (touch, pain, temperature) innervation to the oral cavity of vertebrates. Gustatory neurons innervate taste buds and project centrally to the rostral nucleus of the solitary tract (NTS), whereas neurons providing general epithelial innervati...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.07.042
更新日期：2007-10-15 00:00:00
abstract：:Syndecan-3 is one of four identified members of a family of transmembrane proteoglycans (the syndecans) that possess highly similar cytoplasmic and transmembrane domains and may function as extracellular matrix receptors and/or low affinity receptors for signaling molecules such as FGF. We previously reported the clon...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1093
更新日期：1995-04-01 00:00:00
abstract：:Xenopus laevis is among the few species that are capable of fully regenerating a lost lens de novo. This occurs upon removal of the lens, when secreted factors from the retina are permitted to reach the cornea epithelium and trigger it to form a new lens. Although many studies have investigated the retinal factors tha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.12.036
更新日期：2014-02-15 00:00:00
abstract：:The Wnt pathways are evolutionarily well-conserved signal transduction pathways that are known to play important roles in all Metazoans investigated to date. Here, we examine the Wnt pathway genes and target genes present in the genome of the echinoderm Strongylocentrotus purpuratus. Analysis of the Wnt genes revealed...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.08.045
更新日期：2006-12-01 00:00:00
abstract：:After induction and specification in the ectoderm, at the border of the neural plate, the neural crest (NC) population leaves its original territory through a delamination process. Soon afterwards, the NC cells migrate throughout the embryo and colonize a myriad of tissues and organs where they settle and differentiat...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2011.12.041
更新日期：2012-06-01 00:00:00
abstract：:Unidirectional blue light directs the rhizoid-thallus axis in the apolar zygotes of Fucus and Pelvetia. Here, it is shown that blue light (but not red light) increased cyclic GMP levels of Pelvetia zygotes by about a factor of 2. When the increase in cyclic GMP was blocked by a guanylyl cyclase inhibitor, photopolariz...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8600
更新日期：1997-07-01 00:00:00
abstract：:External genital development begins with formation of paired genital swellings, which develop into the genital tubercle. Proximodistal outgrowth and axial patterning of the genital tubercle are coordinated to give rise to the penis or clitoris. The genital tubercle consists of lateral plate mesoderm, surface ectoderm,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0668
更新日期：2002-07-01 00:00:00
abstract：:The cranial base is a component of the neurocranium and has a central role in the structural integration of the face, brain and vertebral column. Consequently, alteration in the shape of the human cranial base has been intimately linked with primate evolution and defective development is associated with numerous human...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.12.024
更新日期：2016-07-15 00:00:00
abstract：:The sensory neurons of the Drosophila wing differentiate during the initial stages of metamorphosis, appearing in the imaginal wing disc as it everts and flattens. These identifiable neurons arise in a stereotyped sequence, and lay down a specific pattern of axon bundles which travel proximally to the CNS. In several ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90044-2
更新日期：1985-04-01 00:00:00
abstract：:The development of the action potential and responses to neurotransmitters have been described for a population of embryonic spinal neurons developing in vivo. A comparable pattern is seen for spinal neurons developing in dissociated cell culture. The impulse appears very early in this developmental sequence, and the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90065-4
更新日期：1984-11-01 00:00:00
abstract：:To understand mechanisms of sex determination, it is important to know the lineage relationships of cells comprising the gonads. For example, in mice, the Y-linked gene Sry triggers differentiation of Sertoli cells from a cell population originating in the coelomic epithelium overlying the nascent gonad that also give...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.09.007
更新日期：2007-02-01 00:00:00
abstract：:The olfactory epithelium (OE) of the mouse provides a unique system for understanding how cell birth and cell death interact to regulate neuron number during development and regeneration. We have examined cell death in the OE in normal adult mice; in adult mice subjected to unilateral olfactory bulbectomy (surgical re...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.0025
更新日期：1995-11-01 00:00:00
abstract：:The remarkable regenerative capacity displayed by plants and various vertebrates, such as amphibians, is largely based on the capability of somatic cells to undergo dedifferentiation. In this process, mature cells reverse their state of differentiation and acquire pluripotentiality--a process preceding not only reentr...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2003.12.027
更新日期：2004-04-01 00:00:00
abstract：:The level of early histone mRNA in Strongylocentrotus purpuratus changes abruptly at 6 hr of development, increasing an average of 10-fold by 9-10.5 hr and then decreasing over 2-fold by 13.5-15 hr. These changes occur when the late embryonic mRNA is still a very minor component of histone gene transcription. The exac...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90340-8
更新日期：1983-07-01 00:00:00
abstract：:In the full grown oocyte of Xenopus laevis, a sodium permeability can be induced by depolarization to positive potential with current injection. Voltage-clamp analysis has shown that depolarization causes a long-lasting modification of the membrane during which voltage-gated sodium channels become functional. In the p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90302-0
更新日期：1983-10-01 00:00:00
abstract：:We have identified cDNA clones encoding a testis-specific poly(A) polymerase, termed TPAP, a candidate molecule responsible for cytoplasmic polyadenylation of preexisting mRNAs in male haploid germ cells. The TPAP gene was most abundantly expressed coincident with the additional elongation of mRNA poly(A) tails in rou...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9894
更新日期：2000-12-01 00:00:00
abstract：:During mammalian spermiogenesis transitional proteins and protamines replace histones on the DNA as the chromatin condenses. While previous studies suggested that histone genes are inactive postmeiotically, we have shown both by steady-state RNA analysis and nuclear run-off transcription assays that histone 2b (H2b) t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90299-6
更新日期：1989-05-01 00:00:00
abstract：:Epidermal growth factor (EGF) expression and branching morphogenesis were inhibited using a 5' 15-mer antisense oligodeoxynucleotide (ODN) directed against EGF precursor mRNA in embryonic mouse lung in culture under chemically defined, serumless conditions. Antisense EGF ODN resulted in > 90% inhibition of EGF immunor...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1213
更新日期：1993-08-01 00:00:00
abstract：:The location, timing and intensity of Nodal signalling are all critical for proper patterning of the vertebrate embryo. Genetic evidence from mouse and zebrafish indicates that EGF-CFC family members are essential for Nodal ligands to signal. However, the Xenopus EGF-CFC, FRL1, has been implicated in Wnt signalling an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.006
更新日期：2006-04-15 00:00:00
abstract：:Copyright ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8954
更新日期：1998-07-15 00:00:00
abstract：:Mesenchyme to epithelium transitions are crucial to embryonic development. The early mouse embryo offers an excellent model to study epithelium formation as during the first three days of development two epithelia are formed, the trophectoderm (TE) and the primitive endoderm (PrE). We have previously shown that PrE ce...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.10.048
更新日期：2008-01-15 00:00:00
abstract：:The lethal(3)discs overgrown (dco) locus of Drosophila melanogaster, located on the third chromosome at cytogenetic position 100A5,6-100B1,2, is necessary for normal development and growth control in the imaginal discs of the larva. Three recessive lethal alleles (dco2, dco3, and dco18) in heteroallelic combinations a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90090-6
更新日期：1990-08-01 00:00:00
