Abstract：
:Elastic fibers are produced during fetal and neonatal periods, and by maturity elastin expression has ceased. Fetal bovine hyaline chondrocytes acquired an elastogenic phenotype within 24 hr after isolation from the tissue, even though the tissue does not produce elastic fibers or tropoelastin mRNA in vivo. By multiple parameters, hyaline chondrocytes produced elastic fibers that were indistinguishable from those made by elastic chondrocytes derived from fetal elastic ear cartilage. The levels of tropoelastin mRNA, secreted protein, and elastic fiber crosslinks as well as the immunostaining and ultrastructural appearance of elastic fibers produced by cultured hyaline chondrocytes were similar if not identical to those of cultured elastic chondrocytes. We also examined the expression of elastin-associated microfibrillar proteins. In intact hyaline cartilage, we did not detect mRNA for fibrillin 5 mRNA and saw only a relatively weak signal for fibrillin 15 mRNA. These microfibrillar products were expressed with tropoelastin in cultured hyaline chondrocytes as well as in intact elastic cartilage and cultured elastic chondrocytes, suggesting that fibrillin 5 and fibrillin 15 are required for elastic fiber formation. In contrast, the levels of microfibrillar-associated glycoprotein mRNA were decreased in both cell types relative to the high expression seen in vivo. These data indicate that conversion to the elastin phenotype includes induction or modulation of all known components of elastic fibers.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Lee KA,Pierce RA,Davis EC,Mecham RP,Parks WCdoi
10.1006/dbio.1994.1140subject
Has Abstractpub_date
1994-05-01 00:00:00pages
241-52issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(84)71140-7journal_volume
163pub_type
杂志文章abstract：:Aurora A is a mitotic kinase essential for cell proliferation. In mice, ablation of Aurora A results in mitotic arrest and pre-implantation lethality, preventing studies at later stages of development. Here we report the effects of Aurora A ablation on embryo patterning at early post-implantation stages. Inactivation ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.08.010
更新日期：2012-11-01 00:00:00
abstract：:Complement components have been implicated in a wide variety of functions including neurogenesis, proliferation, cell migration, differentiation, cancer, and more recently early development and regeneration. Following our initial observations indicating that C3a/C3aR signaling induces chick retina regeneration, we ana...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.05.019
更新日期：2017-08-01 00:00:00
abstract：:The distribution and ontogeny of four neuropeptides in developing chick lumbosacral sensory and sympathetic ganglia were studied using immunohistochemical techniques. Antibodies to two of these peptides, substance P (SP) and calcitonin gene-related peptide (CGRP), stained small neurons in the medial part of the dorsal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90137-5
更新日期：1986-08-01 00:00:00
abstract：:Expression of the c-abl proto-oncogene in the mouse testis is characterized by the production of a unique 4.7-kb transcript present in germ cells that have entered the haploid phase of spermatogenesis. A similar developmental stage specificity of expression is observed for a member of the 70-kDa heat-shock protein (hs...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90222-9
更新日期：1988-02-01 00:00:00
abstract：:While Wnt signaling is known to be involved in early steps of neural crest development, the mechanism remains unclear. Because Wnt signaling is able to posteriorize anterior neural tissues, neural crest induction by Wnts has been proposed to be an indirect consequence of posteriorization of neural tissues rather than ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.12.016
更新日期：2005-03-01 00:00:00
abstract：:We cloned Xenopus Strabismus (Xstbm), a homologue of the Drosophila planar cell or tissue polarity gene. Xstbm encodes four transmembrane domains in its N-terminal half and a PDZ-binding motif in its C-terminal region, a structure similar to Drosophila and mouse homologues. Xstbm is expressed strongly in the deep cell...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0673
更新日期：2002-07-01 00:00:00
abstract：:Caudal homeobox genes are found across animals, typically linked to two other homeobox genes in what has been called the ParaHox cluster. These genes have been proposed to pattern the anterior-posterior axis of the endoderm ancestrally, but the expression of Caudal in extant groups is varied and often occurs in other ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.10.010
更新日期：2021-02-01 00:00:00
abstract：:Superior cervical ganglia of postnatal mice with a targeted disruption of the gene for neurotrophin-3 have 50% fewer neurons than those of wild-type mice. In culture, neurotrophin-3 increases the survival of proliferating sympathetic precursors. Both precursor death (W. ElShamy et al., 1996, Development 122, 491-500) ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9269
更新日期：1999-06-15 00:00:00
abstract：:In higher organisms, mononucleated myoblasts fuse to form multinucleated myotubes. During this process, myoblasts undergo specific changes in cell morphology and cytoarchitecture. Previously, we have shown that the actin regulator Kette (Hem-2/Nap-1) is essential for myoblast fusion. In this study, we describe the rol...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.01.015
更新日期：2007-04-15 00:00:00
abstract：:In the full grown oocyte of Xenopus laevis, a sodium permeability can be induced by depolarization to positive potential with current injection. Voltage-clamp analysis has shown that depolarization causes a long-lasting modification of the membrane during which voltage-gated sodium channels become functional. In the p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90302-0
更新日期：1983-10-01 00:00:00
abstract：:TGF-beta (TGFβ) family mediated Smad signaling is involved in mesoderm and endoderm specifications, left-right asymmetry formation and neural tube development. The TGFβ1/2/3 and Activin/Nodal signal transduction cascades culminate with activation of SMAD2 and/or SMAD3 transcription factors and their overactivation are...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.09.025
更新日期：2014-12-01 00:00:00
abstract：:Metazoan replication-dependent histone mRNAs accumulate to high levels during S phase as a result of an increase in the rate of histone gene transcription, pre-mRNA processing, and mRNA stability at the G1-S transition. However, relatively little is known about the contribution of these processes to histone expression...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.06.019
更新日期：2004-10-01 00:00:00
abstract：:An important developmental question concerns whether neurotransmitter phenotype is an inherent property of neurons or is influenced by target tissues. This issue can be addressed in the avian ciliary ganglion (CG) which contains two cholinergic populations, ciliary and choroid neurons, that differentially express the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8998
更新日期：1998-10-15 00:00:00
abstract：:The mouse protamine mRNAs, Prm-1 and Prm-2, are translationally repressed for several days during male germ cell differentiation. The translational delay of mouse Prm-1 mRNA has previously been shown to be dependent upon cis-acting elements that reside in the last 62 nucleotides of the Prm-1 3' untranslated region (3'...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8705
更新日期：1997-11-01 00:00:00
abstract：:The individual blastomeres of the preimplantation mouse embryo become polarized during the 8-cell stage. Microvilli become restricted to the free surface of the embryo and this region of the membrane shows increased labeling with FITC-Con A and trinitrobenzenesulfonate (TNBS). Previous studies have shown that this pol...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90204-6
更新日期：1986-04-01 00:00:00
abstract：:This first study of the onset of spermatogenesis in the sea urchin, Strongylocentrotus purpuratus, was undertaken using individuals reared in the laboratory. Spermatogenesis commences about 11-12 months after metamorphosis in these animals. Bindin message accumulates in late spermatocytes and early spermatids which li...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90149-d
更新日期：1990-11-01 00:00:00
abstract：:This study evaluated Na/K-ATPase alpha 1- and alpha 3-subunit isoform polypeptide expression and localization during bovine preattachment development. Na/K-ATPase cation transport activity from the one-cell to blastocyst stage was also determined by measuring ouabain-sensitive 86Rb+ uptake. Both alpha1- and alpha 3-su...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8874
更新日期：1998-05-01 00:00:00
abstract：:The effect of epidermal growth factor (EGF) on cellular differentiation of the neonatal mouse mandibular incisor was examined autoradiographically using tritiated thymidine ([3H]TDR) and tritiated proline ([3H]PRO). On days 0 (day of birth), 1, and 2, EGF was administered (3 micrograms/g body wt) sc to neonates. Mice ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90506-9
更新日期：1987-12-01 00:00:00
abstract：:Retinal ganglion cells (RGCs) are the first cell type to differentiate during retinal histogenesis. It has been postulated that specified RGCs subsequently influence the number and fate of the remaining progenitors to produce the rest of the retinal cell types. However, several genetic knockout models have argued agai...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.01.015
更新日期：2008-04-15 00:00:00
abstract：:We have used a subtractive method to clone novel messages enriched in the heart. Here we show that one such message, bves (blood vessel/epicardial substance) is a novel protein that is highly conserved between chicken and mouse. The bves message is detected at high levels in early chick hearts. Using anti-Bves antibod...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9246
更新日期：1999-05-01 00:00:00
abstract：:Egg yolk phosvitin is one of the most highly phosphorylated extracellular matrix proteins known in nature with unique physico-chemical properties deemed to be critical during ex-vivo egg embryo development. We have utilized our unique live mouse calvarial bone organ culture models under conditions which dissociates th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.06.005
更新日期：2013-09-01 00:00:00
abstract：:A major cause for vision impairment and blindness in industrialized countries is the loss of the light-sensing retinal tissue in the eye. Photoreceptor damage is one of the main characteristics found in retinal degeneration diseases, such as Retinitis Pigmentosa or age-related macular degeneration. The lack of effecti...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2017.09.028
更新日期：2018-01-15 00:00:00
abstract：:In the nematode Caenorhabditis elegans, the BMP-related growth factor DBL-1 regulates body size and male tail morphogenesis via a conserved receptor/Smad signaling pathway. Smads are transcription factors, but rely on transcription cofactors for appropriate regulation of target genes in response to TGF-beta- and BMP-r...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.02.038
更新日期：2007-05-15 00:00:00
abstract：:Mf1, which encodes a winged-helix/forkhead transcription factor, is the murine homolog of human FKHL7, mutated in individuals with autosomal dominant inherited dysgenesis of the anterior segment of the eye (Axenfeld-Reiger anomaly). Mouse embryos homozygous for null mutations in Mf1 (Mf1(lacZ) and Mf1(ch)) show severe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9314
更新日期：1999-07-15 00:00:00
abstract：:Individual somatic muscles and heart progenitors are specified at defined positions within the mesodermal layer of Drosophila. The expression of the homeobox gene even-skipped (eve) identifies one specific subset of cells in the dorsal mesoderm, which give rise to particular pericardial cells and dorsal body wall musc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0397
更新日期：2001-10-01 00:00:00
abstract：:The tadpole-type larva of Ciona has emerged as an intriguing model system for the study of neurodevelopment. The Ciona intestinalis connectome has been recently mapped, revealing the smallest central nervous system (CNS) known in any chordate, with only 177 neurons. This minimal CNS is highly reminiscent of larger CNS...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.09.023
更新日期：2019-04-15 00:00:00
abstract：:This work describes unknown aspects of chordotonal organ (ChO) morphogenesis revealed in post-embryonic stages through the use of new fluorescently labeled markers. We show that towards the end of embryogenesis a hitherto unnoticed phase of cell migration commences in which the cap cells of the ventral ChOs elongate a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.01.009
更新日期：2016-03-01 00:00:00
abstract：:Fertilized eggs of the leech Helobdella triserialis undergo a cytoplasmic reorganization which generates domains of nonyolky cytoplasm, called teloplasm, at the animal and vegetal poles. The segregation of teloplasm to one cell of the eight-cell embryo is responsible for a unique developmental fate of that cell, i.e.,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90181-4
更新日期：1989-10-01 00:00:00
abstract：:The myelin of the peripheral nervous system from the shiverer mutant mice is characterized by the absence of myelin basic protein, while the other myelin protein components are present at normal levels. Myelin lamella formation is normal in the shiverer mutant. Therefore, by using antiserum against myelin basic protei...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90277-x
更新日期：1984-09-01 00:00:00
abstract：:Previous work has documented the importance of BMPs in eye development. Loss-of-function studies in mice, with targeted deletions in either the Bmp7 or Bmp4 genes, have shown that these molecules are critical for early eye development. On the basis of the asymmetry in the dorsal-ventral expression patterns of several ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00115-x
更新日期：2003-04-01 00:00:00
