Abstract：
:The individual blastomeres of the preimplantation mouse embryo become polarized during the 8-cell stage. Microvilli become restricted to the free surface of the embryo and this region of the membrane shows increased labeling with FITC-Con A and trinitrobenzenesulfonate (TNBS). Previous studies have shown that this polarity develops in response to asymmetric cell-cell contact with stage specific induction competent blastomeres. In the present study, the ability of later stage embryos to induce 8-cell polarization has been investigated. Newly-formed, nonpolar 8-cell stage blastomeres (1/8 cells) were isolated, then aggregated with morulae, inner cell clusters (from morulae), blastocysts, or inner cell masses (ICM) and cultured for 8 hr. Aggregates were then assayed for polarity. The results show a hierarchy of inducing ability, with the ICM and IC cluster possessing greater activity than the morula and polar trophectoderm of the early blastocyst, while the mural trophectoderm shows very little inducing activity. Furthermore, the inducing ability of the polar trophectoderm decreases with complete expansion and hatching of the blastocyst. These results indicate that the ability to induce 8-cell blastomere polarization is retained by the embryo beyond the 8-cell stage and that this ability is lost with further differentiation.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Adler RR,Ziomek CAdoi
10.1016/0012-1606(86)90204-6subject
Has Abstractpub_date
1986-04-01 00:00:00pages
395-402issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(86)90204-6journal_volume
114pub_type
杂志文章abstract：:Effective transgenesis methods have been successfully employed in many organisms including zebrafish. However, accurate spatiotemporal control of transgene expression is still difficult to achieve. Here we describe a system for chemical-inducible gene expression and demonstrate its feasibility for generating transgeni...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.042
更新日期：2008-08-01 00:00:00
abstract：:The origin of the avian cardiovascular system from the primitive streak has been mapped by the construction of quail/chick transplantation chimeras, the use of QH-1 (an antiquail endothelial/endocardial cell marker), and injections of a vital fluorescent dye (DiI) into the primitive streak. Our studies reveal that the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1276
更新日期：1993-10-01 00:00:00
abstract：:Hox genes encode transcription factors which provide positional information during morphogenesis along the body axis; genetic interaction among Hox genes is thought to be necessary for correct pattern formation. One of the most curious features of the 39 vertebrate Hox genes is that they form four clusters each compos...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9651
更新日期：2000-04-15 00:00:00
abstract：:Octopamine is a major monoamine in invertebrates and affects many physiological processes ranging from energy metabolism to complex behaviors. Octopamine binds to receptors located on various cell types and activates distinct signal transduction pathways to produce these diverse effects. We previously identified one o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.07.018
更新日期：2003-12-01 00:00:00
abstract：:We have investigated the function of the neurogenic gene Notch (N) during development of the adult sensilla of Drosophila. Heat pulses were applied to flies carrying the temperature-sensitive Notch allele Nts1 at different larval and pupal stages. We can show that the reduction of Notch+ function during a short interv...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90147-b
更新日期：1990-11-01 00:00:00
abstract：:We report here the identification and characterization of a novel gene, T1 alpha, expressed in high abundance in adult rat lung, fetal lung, and early fetal brain. T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. The cDNA for...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1024
更新日期：1995-01-01 00:00:00
abstract：:In the developing retina, as in other regions of the CNS, neural progenitors give rise to individual cell types during discrete temporal windows. Pax6 is expressed in retinal progenitor cells (RPCs) throughout the course of retinogenesis, and has been shown to be required during early retinogenesis for generation of m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.09.030
更新日期：2017-12-01 00:00:00
abstract：:Pronuclear migration and formation of the first mitotic spindle depend upon assembly of a functional zygotic centrosome. For most animals, this involves both paternal and maternal contributions as sperm basal bodies are converted into centrosomes competent for microtubule nucleation through recruitment of egg proteins...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.10.001
更新日期：2004-01-15 00:00:00
abstract：:Three dominant second-chromosome rearrangement mutations in Drosophila melanogaster, Aristapedioid1 (Arp), vestigial-Depilate (vgD), and vestigial62 (vg62), result in developmental abnormalities of the bristle sense organs on the notum, abdomen, legs, and wing margin. The bristle abnormalities are associated with over...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1039
更新日期：1994-02-01 00:00:00
abstract：:To analyse cell migration and the differentiation potential of migratory stem cells in Hydractinia, we generated animals with an eGFP reporter gene stably expressed and transmitted via the germline. The transgene was placed under the control of two different actin promoters and the promoter of elongation factor-1α. On...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.08.017
更新日期：2010-12-01 00:00:00
abstract：:The location, timing and intensity of Nodal signalling are all critical for proper patterning of the vertebrate embryo. Genetic evidence from mouse and zebrafish indicates that EGF-CFC family members are essential for Nodal ligands to signal. However, the Xenopus EGF-CFC, FRL1, has been implicated in Wnt signalling an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.006
更新日期：2006-04-15 00:00:00
abstract：:GATA4, a transcription factor expressed in the proximal small intestine but not in the distal ileum, maintains proximal-distal distinctions by multiple processes involving gene repression, gene activation, and cell fate determination. Friend of GATA (FOG) is an evolutionarily conserved family of cofactors whose member...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.07.022
更新日期：2008-10-01 00:00:00
abstract：:Retinoic acid (RA) plays a critical role in neural patterning and organogenesis in the vertebrate embryo. Here we characterize a mutant of the zebrafish named giraffe (gir) in which the gene for the RA-degrading enzyme Cyp26a1 is mutated. The gir mutant displayed patterning defects in multiple organs including the com...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.11.023
更新日期：2005-02-15 00:00:00
abstract：:Spermatogonia are adult germline stem cells that can both self-renew and differentiate into spermatocytes. Little is known about factors necessary for spermatogonial differentiation. We identified a novel germ-cell-specific transcription factor that we named Sohlh1 (testis and ovary expressed basic helix-loop-helix (b...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.02.027
更新日期：2006-06-01 00:00:00
abstract：:The segmental origin and migratory pattern of neural crest cells at the trunk level of avian embryos was studied, with special emphasis on the formation of the dorsal root ganglia (DRG) which organize in the anterior half of each somite. Neural crest cells were visualized using the quail-chick marker and HNK-1 immunof...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90236-3
更新日期：1987-04-01 00:00:00
abstract：:This study tested the proposition that Na/K-ATPase activity could be involved in the morphogenetic aspects of mouse blastocyst formation by facilitating the localization of certain organelles to apposed borders, the production of nascent blastocoele fluid, and cavitation. It was assumed that such Na/K-ATPase activity ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90290-2
更新日期：1984-10-01 00:00:00
abstract：:Clotting is critical in limiting hemolymph loss and initiating wound healing in insects as in vertebrates. It is also an important immune defense, quickly forming a secondary barrier to infection, immobilizing bacteria and thereby promoting their killing. However, hemolymph clotting is one of the least understood immu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.03.019
更新日期：2006-07-01 00:00:00
abstract：:The murine homeobox genes Msx-1 and Msx-2 are related to the Drosophila msh gene and are expressed in a variety of tissues during mouse embryogenesis. We now report the developmentally regulated expression of Msx-1 and Msx-2 in the mouse mammary gland and show that their expression patterns point toward significant fu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0168
更新日期：1996-07-10 00:00:00
abstract：:Skeletal muscle development (myogenesis) is a complex but precisely orchestrated process involving spatiotemporal regulation of the proliferation, differentiation and fusion of myogenic progenitor cells (myoblasts). Here we identify brain expressed x-linked gene 1 (Bex1) as a transient, developmentally regulated gene ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.11.007
更新日期：2016-01-01 00:00:00
abstract：:Mutations in the Pax6 gene disrupt telencephalic development, resulting in a thin cortical plate, expansion of proliferative layers, and the absence of the olfactory bulb. The primary defect in the neuronal cell population of the developing cerebral cortex was analysed by using mouse chimeras containing a mixture of w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00046-5
更新日期：2003-03-01 00:00:00
abstract：:Mutations that inactivate either merlin (mer) or expanded (ex) result in increased cell growth and proliferation in Drosophila. Both Mer and Ex are members of the Band 4.1 protein superfamily, and, based on analyses of mer ex double mutants, they are proposed to function together in at least a partially redundant mann...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.12.021
更新日期：2007-04-01 00:00:00
abstract：:To understand cell interactions during induction of skin appendages, we studied the roles of adhesion molecules N-CAM, tenascin, integrin, and fibronectin during feather development. Tenascin appeared in a periodic pattern on epithelia and was so far the earliest molecule detected in placodes. Three placode domains we...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90009-6
更新日期：1992-03-01 00:00:00
abstract：:The segmentation of somites from the paraxial mesoderm is a crucial event in vertebrate embryonic development; however, the mechanisms underlying this process are not well understood. In a yeast two-hybrid screen we have identified the novel basic-helix-loop-helix (bHLH) protein cMeso-1 which is expressed in the preso...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8919
更新日期：1998-07-15 00:00:00
abstract：:Skeletal muscle fibers express members of the myosin heavy chain (MyHC) gene family in a fiber-type-specific manner. In avian skeletal muscle it is the expression of the slow MyHC isoforms that most clearly distinguishes slow- from fast-contracting fiber types. Two hypotheses have been proposed to explain fiber-type-s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8619
更新日期：1997-08-01 00:00:00
abstract：:CREB-2 (also called ATF4, TAXREB67, or C/ATF) is an evolutionarily conserved member of the CREB/ATF family of basic-leucine zipper transcription factors. CREB-2 is expressed ubiquitously in the adult mouse and can function as both a transcriptional activator and a repressor. However, little was understood about the no...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9699
更新日期：2000-06-01 00:00:00
abstract：:The location and distribution of neural crest-derived Schwann cells during development of the peripheral nerves of chick forelimbs were examined using chick-quail chimeras. Neural crest cells were labeled by transplantation of the dorsal part of the neural tube from a quail donor to a chick host at levels of the neura...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90014-8
更新日期：1992-03-01 00:00:00
abstract：:Selected stages of the developing chicken cornea have been examined for type VI collagen, employing monoclonal antibodies specific for this molecule. By immunofluorescence, the molecule is not detectable in 5 1/2 day corneas, a time at which the epithelial-derived, acellular primary stroma is the only corneal matrix p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90013-8
更新日期：1986-12-01 00:00:00
abstract：:Mesenchyme to epithelium transitions are crucial to embryonic development. The early mouse embryo offers an excellent model to study epithelium formation as during the first three days of development two epithelia are formed, the trophectoderm (TE) and the primitive endoderm (PrE). We have previously shown that PrE ce...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.10.048
更新日期：2008-01-15 00:00:00
abstract：:Similar amounts of expression of a luciferase reporter gene that is driven by the SV40 early promoter are detected in G2 of 1-cell mouse embryo following injection of the male pronucleus in either early or mid/late S. No luciferase activity is observed when the male pronucleus is injected in early S and the embryos an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1101
更新日期：1993-04-01 00:00:00
abstract：:Cells that overexpress PKA as a consequence of carrying multiple copies of the gene encoding the catalytic subunit can be induced to sporulate when developing as single cells. A peptide phosphorylated by PKA, termed SDF-1, has recently been shown to stimulate this process (Anjard et al., 1997). Several genes have been...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8804
更新日期：1998-01-15 00:00:00
