Abstract：
:Aurora A is a mitotic kinase essential for cell proliferation. In mice, ablation of Aurora A results in mitotic arrest and pre-implantation lethality, preventing studies at later stages of development. Here we report the effects of Aurora A ablation on embryo patterning at early post-implantation stages. Inactivation of Aurora A in the epiblast or visceral endoderm layers of the conceptus leads to apoptosis and inhibition of embryo growth, causing lethality and resorption at approximately E9.5. The effects on embryo patterning, however, depend on the tissue affected by the mutation. Embryos with an epiblast ablation of Aurora A properly establish the anteroposterior axis but fail to progress through gastrulation. In contrast, mutation of Aurora A in the visceral endoderm, leads to posteriorization of the conceptus or failure to elongate the anteroposterior axis. Injection of ES cells into Aurora A epiblast knockout blastocysts reconstitutes embryonic development to E9.5, indicating that the extra-embryonic tissues in these mutant embryos can sustain development to organogenesis stages. Our results reveal new ways to induce apoptosis and to ablate cells in a tissue-specific manner in vivo. Moreover, they show that epiblast-ablated embryos can be used to test the potency of stem cells.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Yoon Y,Cowley DO,Gallant J,Jones SN,Van Dyke T,Rivera-Pérez JAdoi
10.1016/j.ydbio.2012.08.010subject
Has Abstractpub_date
2012-11-01 00:00:00pages
77-85issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(12)00441-1journal_volume
371pub_type
杂志文章abstract：:Interleukin-6 is a member of a class of hormone-like molecules termed cytokines. The actions of IL-6 are highly pleiotropic. In adults IL-6 functions as a major mediator of inflammatory responses as well as inducing the synthesis of acute phase proteins by the liver following infection or injury. Based on in vitro and...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/0012-1606(92)90173-e
更新日期：1992-06-01 00:00:00
abstract：:We studied the pathway of 5S ribosomal RNA (rRNA) during oogenesis in Xenopus from its storage in the cytoplasm to incorporation into ribosomes in the nucleus. Ribonucleoprotein particle (RNP) assembly assays reveal striking differences in the behavior of oocyte-type and somatic-type 5S rRNA after microinjection into ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1080
更新日期：1995-04-01 00:00:00
abstract：:The HNK-1 antibody recognizes a carbohydrate moiety that is shared by a family of cell adhesion molecules and is also present on the surface of migrating neural crest cells. Here, the effects of the HNK-1 antibody on neural crest cells were examined in vitro and in vivo. When the HNK-1 antibody was added to neural tub...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90390-3
更新日期：1987-10-01 00:00:00
abstract：:The formation of the spinal cord during early embryonic development in vertebrate embryos is a continuous process that begins at gastrulation and continues through to the completion of somitogenesis. Despite the conserved usage of patterning mechanisms and gene regulatory networks that act to generate specific spinal ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2017.01.021
更新日期：2017-12-01 00:00:00
abstract：:The relationship between cellular position and growth control has been studied in cultures of dissociated fragments of mouse limb bud cells. Using cells derived from various positions along the anterior-posterior axis of the limb bud we have developed culture conditions that optimize growth of positionally isolated ce...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90208-x
更新日期：1992-05-01 00:00:00
abstract：:MicroRNAs (miRNAs) are post-transcriptional regulators of growth and development in both plants and animals. Flowering is critical for the reproduction of angiosperms. Flower development entails the transition from vegetative growth to reproductive growth, floral organ initiation, and the development of floral organs....
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2013.05.009
更新日期：2013-08-15 00:00:00
abstract：:Previous studies have suggested a role of the homeodomain Six family proteins in patterning the developing vertebrate head that involves appropriate segmentation of three tissue layers, the endoderm, the paraxial mesoderm and the neural crest cells; however, the developmental programs and mechanisms by which the Six g...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.12.015
更新日期：2006-05-15 00:00:00
abstract：:Integrin α5-null embryos die in mid-gestation from severe defects in cardiovascular morphogenesis, which stem from defective development of the neural crest, heart and vasculature. To investigate the role of integrin α5β1 in cardiovascular development, we used the Mesp1(Cre) knock-in strain of mice to ablate integrin ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.09.014
更新日期：2014-11-15 00:00:00
abstract：:Stimulation of epithelial cell cycling by the neuropeptide head activator was analyzed in Hydra magnipapillata and compared with the action of head activator on bud formation and tentacle formation during head regeneration. The results obtained indicate that head activator treatment stimulates epithelial cell division...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.8491
更新日期：1997-03-01 00:00:00
abstract：:The Drosophila brain is generated by a complex series of morphogenetic movements. To better understand brain development and to provide a guide for experimental manipulation of brain progenitors, we created a fate map using photoactivated gene expression to mark cells originating within specific mitotic domains and ti...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00220-3
更新日期：2003-08-01 00:00:00
abstract：:The MEI-1/MEI-2 microtubule-severing complex, katanin, is required for oocyte meiotic spindle formation and function in C. elegans, but the microtubule-severing activity must be quickly downregulated so that it does not interfere with formation of the first mitotic spindle. Post-meiotic MEI-1 inactivation is accomplis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.04.004
更新日期：2009-06-15 00:00:00
abstract：:Actin dynamics are necessary at multiple steps in the formation of multinucleated muscle cells. BAR domain proteins can regulate actin dynamics in several cell types, but have been little studied in skeletal muscle. Here, we identify novel functions for the N-BAR domain protein, Bridging integrator 3 (Bin3), during my...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.07.004
更新日期：2013-10-01 00:00:00
abstract：:The epithelial-mesenchymal transition of cardiac endothelium is a critical developmental event in the formation of valvular and septal anlagen. We have demonstrated previously that this event can be mimicked in culture by treating atrioventricular canal (AV) endothelium with EDTA-soluble proteins extracted from embryo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90121-i
更新日期：1991-06-01 00:00:00
abstract：:The cDNA encoding the TATA binding protein was isolated from 8- to 16-cell and morula-stage embryonic libraries of two distantly related species of sea urchin, Strongylocentrotus purpuratus and Lytechinus variegatus, respectively. The two proteins are 96% identical over both the N- and C-terminal domains, suggesting a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9052
更新日期：1998-12-01 00:00:00
abstract：:Although mitogen-activated protein kinase (MAPK) is a well-known cell cycle regulator, emerging studies have also implicated its activity in the regulation of intracellular calcium concentration ([Ca2+](i)) and secretion. Those studies raise the hypothesis that MAPK activity during oocyte maturation and early fertiliz...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.03.029
更新日期：2007-06-15 00:00:00
abstract：:Lineage specification of multipotent progenitor cells is governed by a balance of lineage-affiliated transcription factors, such as GATA1 and PU.1, which regulate the choice between erythroid and myelomonocytic fates. But how ratios of lineage-determining transcription factors stabilize progenitor cells and resolve th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.02.036
更新日期：2007-05-15 00:00:00
abstract：:A glycoprotein from the liver, which shares epitopes with chorion (egg envelope or zona pellucida) glycoproteins, is present only in the spawning female fish, Oryzias latipes, under natural conditions. This spawning female-specific (SF) substance is distinct from vitellogenin but closely resembles a major glycoprotein...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90301-1
更新日期：1989-05-01 00:00:00
abstract：:Wnt signaling is essential to many events during organogenesis, including the development of the mammalian lung. The Wnt family member Wnt4 has been shown to be required for the development of kidney, gonads, thymus, mammary and pituitary glands. Here, we show that Wnt4 is critical for proper morphogenesis and growth ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.08.017
更新日期：2015-10-15 00:00:00
abstract：:Using lineage tracers, we recently showed dorsal and ventral ectodermal compartments along the sides of the body in chick embryos. The compartments are formed both in presumptive limb-forming regions where they position the apical ridge and also in presumptive interlimb (flank). Here we show, using a novel technique c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9659
更新日期：2000-06-15 00:00:00
abstract：:To examine whether the extracellular matrix (ECM) plays a role in mediating interactions between the apical ectodermal ridge (AER) and the subjacent mesoderm in the chick wing bud, we used immunohistochemistry to locate the following tissue components during wing morphogenesis: types I and IV collagens, fibronectin, t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1141
更新日期：1994-05-01 00:00:00
abstract：:Previous studies have established that embryonal carcinoma (EC) cells produce several different growth factors, but express few, if any, receptors for epidermal growth factor, platelet-derived growth factor, or transforming growth factor type-beta. In this study, the production and utilization of fibroblast growth fac...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90161-3
更新日期：1988-09-01 00:00:00
abstract：:The calmodulin levels in stage 6 Xenopus oocytes averaged 89 +/- 24 (SD) ng/oocyte and had largely accumulated by stage 3 of oogenesis. From stage 3 to early stage 6, calmodulin levels did not increase further. However, in large stage 6 oocytes (greater than 1.25 mm diam) calmodulin levels again rose to a level as hig...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90120-x
更新日期：1986-01-01 00:00:00
abstract：:Cardiac troponin T (cTnT) is a component of the troponin (Tn) complex in cardiac myocytes, and plays a regulatory role in cardiac muscle contraction by anchoring two other Tn components, troponin I (TnI) and troponin C, to tropomyosin (Tm) on the thin filaments. In order to determine the in vivo function of cTnT, we c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.07.007
更新日期：2008-10-01 00:00:00
abstract：:In this report we describe the development of a sensitive assay for gene expression in zebrafish embryos using beta-lactamase as a reporter gene. We show that injection of a green fluorescent substrate for beta-lactamase allows the detection of reporter gene expression in live embryos. The beta-lactamase enzyme cataly...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8999
更新日期：1998-11-15 00:00:00
abstract：:FOG-2 is a multi-zinc finger protein that binds the transcriptional activator GATA4 and modulates GATA4-mediated regulation of target genes during heart development. Our previous work has demonstrated that the Nucleosome Remodeling and Deacetylase (NuRD) complex physically interacts with FOG-2 and is necessary for FOG...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.08.030
更新日期：2014-11-01 00:00:00
abstract：:We have examined the roles of cell lineage and interactions in the determination of individual identified neurons in the grasshopper embryo by selective ablations of individual cells and/or their neighbors at successive stages following their birth. The neurons in the grasshopper central nervous system (CNS) are produ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90069-7
更新日期：1985-07-01 00:00:00
abstract：:Calcium influx is known to be prerequisite for membrane fusion of myoblasts. However, little is known about the channels that are responsible for the entry of calcium into the cells. Here we show that K(Ca) channels and stretch-activated channels are involved in the calcium influx. Upon analysis of single-channel reco...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0091
更新日期：1996-04-10 00:00:00
abstract：:The unc-52 gene of Claenorhabditis elegans encodes a homologue of the basement membrane heparan sulfate proteoglycan perlecan. Viable alleles reduce the abundance of UNC-52 in late larval stages and increase the frequency of distal tip cell (DTC) migration defects caused by mutations disrupting the UNC-6/netrin guidan...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00014-9
更新日期：2003-04-01 00:00:00
abstract：:Bone development is dependent on the functionality of three essential cell types: chondrocytes, osteoclasts and osteoblasts. If any of these cell types is dysfunctional, a developmental bone phenotype can result. The bone disease osteopetrosis is caused by osteoclast dysfunction or impaired osteoclastogenesis, leading...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.06.018
更新日期：2010-08-15 00:00:00
abstract：:Using sea urchin embryos, we demonstrate that the MEK/MAPK/ERK cascade is essential for the proper progression of the cell cycle. Activation of a limited fraction of MAPK/ERK is required between S-phase and M-phase. Neither DNA replication nor CDK1 activation are impacted by the inhibition of this small active MAPK/ER...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.11.018
更新日期：2017-01-15 00:00:00
