Abstract:
:The MEI-1/MEI-2 microtubule-severing complex, katanin, is required for oocyte meiotic spindle formation and function in C. elegans, but the microtubule-severing activity must be quickly downregulated so that it does not interfere with formation of the first mitotic spindle. Post-meiotic MEI-1 inactivation is accomplished by two parallel protein degradation pathways, one of which requires MEL-26, the substrate-specific adaptor that recruits MEI-1 to a CUL-3 based ubiquitin ligase. Here we address the question of how MEL-26 mediated MEI-1 degradation is triggered only after the completion of MEI-1's meiotic function. We find that MEL-26 is present only at low levels until the completion of meiosis, after which protein levels increase substantially, likely increasing the post-meiotic degradation of MEI-1. During meiosis, MEL-26 levels are kept low by the action of another type of ubiquitin ligase, which contains CUL-2. However, we find that the low levels of meiotic MEL-26 have a subtle function, acting to moderate MEI-1 activity during meiosis. We also show that MEI-1 is the only essential target for MEL-26, and possibly for the E3 ubiquitin ligase CUL-3, but the upstream ubiquitin ligase activating enzyme RFL-1 has additional essential targets.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Johnson JL,Lu C,Raharjo E,McNally K,McNally FJ,Mains PEdoi
10.1016/j.ydbio.2009.04.004subject
Has Abstractpub_date
2009-06-15 00:00:00pages
349-57issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(09)00236-Xjournal_volume
330pub_type
杂志文章abstract::In the course of embryogenesis multicellular structures and organs are assembled from constituent cells. One structural component common to many organs is the tube, which consists most simply of a luminal space surrounded by a single layer of epithelial cells. The notochord of ascidian Ciona forms a tube consisting of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.03.015
更新日期:2009-06-15 00:00:00
abstract::The relative sizes of the various structures in Hydra attenuata were compared over a broad range of animal sizes to determine in detail the ability to regulate proportions during regeneration. The three components of the head, namely hypostome, tentacles, and tentacle zone from which the tentacles emerge, the body col...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90318-x
更新日期:1984-06-01 00:00:00
abstract::The contributions that control of cell proliferation and cell growth make to developmental regulation of organ and body size remain poorly explored, particularly with respect to endocycles in polyploid tissues. The epithelium of the marine chordate Oikopleura dioica is composed of a fixed number of cells grouped in te...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0834
更新日期:2002-12-01 00:00:00
abstract::The facial primordia in the chick embryo begin as rounded swellings that surround the primitive mouth and these grow out to form the beak. The control of proximodistal outgrowth is not well understood but may involve similar mechanisms to the limb bud. In order to test this hypothesis, combinations were made between e...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90069-s
更新日期:1992-12-01 00:00:00
abstract::Suppression of wing fate and specification of haltere fate in Drosophila by the homeotic gene Ultrabithorax is a classical example of Hox regulation of serial homology (Lewis, E.B. 1978. Nature 276, 565-570) and has served as a paradigm for understanding homeotic gene function. We have used DNA microarray analyses to ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.12.022
更新日期:2006-03-15 00:00:00
abstract::During development and maturation, mammalian oocytes are surrounded by the zona pellucida which in the mouse is comprised of three sulfated glycoproteins, ZP-1, ZP-2, and ZP-3. Previously, monoclonal antibodies to ZP-2 have been isolated. The isolation and characterization of monoclonal antibodies specific for ZP-3, t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90454-3
更新日期:1985-06-01 00:00:00
abstract::In tetrapods, the anteroposterior (AP) patterning of the limb is under the control of the antagonistic activities of the secreted factor Sonic hedgehog (Shh) and Gli3R, the truncated repressor form of the transcription factor Gli3. In this report, we show that Msx1 and Msx2 are targets and downstream effectors of Gli3...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.04.038
更新日期:2009-07-15 00:00:00
abstract::The ubiquitin ligase Smurf1 can target a handful of signaling proteins for ubiquitin-mediated proteasomal destruction or functional modification, including TGF-beta receptors, Smads, transcription factors, RhoA and MEKK2. Smurf1 was initially implicated in BMP pathway regulation in embryonic development, but its poten...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.08.009
更新日期:2006-11-15 00:00:00
abstract::Joint and skeletal development is highly regulated by extracellular matrix (ECM) proteoglycans, of which chondroitin sulfate proteoglycans (CSPGs) are a major class. Despite the requirement of joint CSPGs for skeletal flexibility and structure, relatively little is understood regarding their role in establishing joint...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.01.005
更新日期:2012-03-15 00:00:00
abstract::We present a genetic map for Xenopus tropicalis, consisting of 2886 Simple Sequence Length Polymorphism (SSLP) markers. Using a bioinformatics-based strategy, we identified unique SSLPs within the X. tropicalis genome. Scaffolds from X. tropicalis genome assembly 2.0 (JGI) were scanned for Simple Sequence Repeats (SSR...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.03.022
更新日期:2011-06-01 00:00:00
abstract::Germ line transformation has been used to map the cis regulatory DNA elements responsible for the precise and evolutionarily stable developmental expression of the s18 chorion gene. Constructs containing chimeric combinations of Drosophila melanogaster and D. grimshawi DNA regions, as well as D. grimshawi sequences al...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90160-i
更新日期:1992-07-01 00:00:00
abstract::Tissue specificity of cell adhesion was directly characterized in a unit cell interaction using a novel laser trapping cell manipulator in combination with a fixed micropipet. We quantified the adhesive specificity of endodermal and ectodermal epithelial cells from Hydra, which are known to sort out within hours after...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1068
更新日期:1994-03-01 00:00:00
abstract::The colonial tunicate Botrylloides leachi can regenerate functional adults from minute vasculature fragments, in a poorly understood phenomenon termed Whole Body Regeneration (WBR). Using Piwi expression (Bl-Piwi), blood cell labeling and electron microscopy, we show that WBR develops through activation, mobilization ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.05.500
更新日期:2010-09-01 00:00:00
abstract::Catecholaminergic cells are transiently present during development of the fetal murine bowel. These transient catecholaminergic (TC) cells appear at Day E10, but by Day E13 can no longer be detected. In order to evaluate the hypothesis that these cells are the precursors of enteric neurons, we investigated the possibi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90217-0
更新日期:1989-03-01 00:00:00
abstract::We have examined the interfaces between protein and lipid sulfation, the response of progenitor cells to specific growth factors, and developmental lineage progression. The experimental system used is the oligodendrocyte (OL), the cell responsible for myelination of axons in the central nervous system. The ability to ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1105
更新日期:1994-04-01 00:00:00
abstract::Directed cell movement is integral to both embryogenesis and hematopoiesis. In the adult, the chemokine family of secreted proteins signals migration of hematopoietic cells through G-coupled chemokine receptors. We detected embryonic expression of chemokine receptor messages by RT-PCR with degenerate primers at embryo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9405
更新日期:1999-09-15 00:00:00
abstract::Differentiation from a haploid round spermatid to a highly streamlined, motile sperm requires temporal and spatial regulation of the expression of numerous proteins. One form of regulation is the storage of translationally repressed mRNAs. In Drosophila spermatocytes, the transcription of many of these translationally...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.02.018
更新日期:2013-05-01 00:00:00
abstract::Zygote arrest (Zar) proteins are crucial for early embryonic development, but their molecular mechanism of action is unknown. The Translational Control Sequence (TCS) in the 3' untranslated region (UTR) of the maternal mRNA, Wee1, mediates translational repression in immature Xenopus oocytes and translational activati...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.06.012
更新日期:2012-09-15 00:00:00
abstract::Lysine methylation of histones is associated with both transcriptionally active chromatin and with silent chromatin, depending on what residue is modified. Histone methyltransferases and demethylases ensure that histone methylations are dynamic and can vary depending on cell cycle- or developmental stage. KDM4A demeth...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.11.011
更新日期:2013-01-15 00:00:00
abstract::The transforming growth factor-beta (TGF-beta) superfamily contains a variety of growth factors which all share common sequence elements and structural motifs. These proteins are known to exert a wide spectrum of biological responses on a large variety of cell types in both vertebrates and invertebrates. Many of them ...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:
更新日期:2002-10-15 00:00:00
abstract::Ephrin-B2, a member of the Eph/ephrin family of cell signaling molecules, has been implicated in the guidance of cranial and trunk neural crest cells (NCC) and development of the branchial arches(BA), but detailed examination in mice has been hindered by embryonic lethality of Efnb2 null loss of function due to a requ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.08.021
更新日期:2015-10-15 00:00:00
abstract::About 12-17% of the embryos obtained by mating mice carrying the In(X)1H or Paf mutations are of the 39,X (X0) genotype. Depending on the mutant mice used for mating, the monosomic X chromosome can be inherited from the paternal (XP) or the maternal (XM) parent. The XP0 embryos display developmental retardation at gas...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8972
更新日期:1998-09-01 00:00:00
abstract::This investigation provides the first systematic determination of the cellular and molecular progression of vocal fold (VF) epithelium development in a murine model. We define five principal developmental events that constitute the progression from VF initiation in the embryonic anterior foregut tube to fully differen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.12.037
更新日期:2015-03-15 00:00:00
abstract::The organizer is an essential signaling center required for axial formation during vertebrate embryonic development. In the basal chordate amphioxus, the dorsal blastopore lip of the gastrula has been proposed to be homologous to the vertebrate organizer. Lefty is one of the first genes to be expressed in the organize...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.08.006
更新日期:2019-12-01 00:00:00
abstract::Prophase-arrested oocytes of Ruditapes philippinarum are triggered to undergo germinal vesicle breakdown under the influence of the neurohormone serotonin (5HT) and then arrest in metaphase 1. Our data show that these oocytes possess a single class of original 5HT receptors. Their binding parameters have been determin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1222
更新日期:1994-08-01 00:00:00
abstract::Choroideremia (CHM) is a hereditary eye disease caused by mutations in the X-linked CHM gene. Disruption of the Chm gene in mice resulted in prenatal death of Chm-/Y males and Chm-/Chm+ females that had inherited the mutation from their mothers. Male chimeras and Chm+/Chm- females with paternal transmission of the mut...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.04.016
更新日期:2004-08-01 00:00:00
abstract::Drosophila melanogaster mature oocytes in ovaries are arrested at metaphase I of meiosis. Eggs that have reached the uterus have released this arrest. It was not known where in the female reproductive tract egg activation occurs and what triggers it. We investigated when and where the egg is activated in Drosophila in...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0246
更新日期:2001-06-15 00:00:00
abstract::Previous study of the development of the levator ani muscle (LA) revealed that the sex difference in muscle fiber number is under the control of testosterone during the perinatal period. In this study, the development of the LA muscle was examined in both sexes to test the hypothesis that testosterone action determine...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1070
更新日期:1994-03-01 00:00:00
abstract::The cranial neural crest (CNC) is a highly motile and multipotent embryonic cell population, which migrates directionally on defined routes throughout the embryo, contributing to facial structures including cartilage, bone and ganglia. Cadherin-mediated cell-cell adhesion is known to play a crucial role in the directi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.02.007
更新日期:2016-03-15 00:00:00
abstract::A gene regulatory network subcircuit comprising the otx, wnt8, and blimp1 genes accounts for a moving torus of gene expression that sweeps concentrically across the vegetal domain of the sea urchin embryo. Here we confirm by mutation the inputs into the blimp1cis-regulatory module predicted by network analysis. Its es...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.10.042
更新日期:2008-01-15 00:00:00