Position-specific growth of mouse limb bud cells in vitro.


:The relationship between cellular position and growth control has been studied in cultures of dissociated fragments of mouse limb bud cells. Using cells derived from various positions along the anterior-posterior axis of the limb bud we have developed culture conditions that optimize growth of positionally isolated cells. Under these conditions limb bud cells display an inherent, position-specific growth response; proliferation of cells derived from anterior and central regions of the limb is enhanced over that of posterior derived cells. Thus, within the total population of limb bud cells the in vitro growth of posterior cells is unique and correlates with the positional activity associated with the zone of polarizing activity. Anterior and posterior cells were cocultured to determine whether interactions between these two groups of positionally distinct cells lead to the stimulation of growth that has been observed in vivo. We observe a slight but consistent position-dependent stimulation of growth that is indicative of a mitogenic signal passing between these positionally disparate cells. Similarities between position-related growth dynamics in vivo and in vitro suggest that positional interactions that are important for limb formation can occur between dissociated cells cultured under standard conditions.


Dev Biol


Developmental biology


Shi C,Muneoka K




Has Abstract


1992-05-01 00:00:00














  • Ultrastructural studies of the ontogeny of fetal human and porcine endocrine pancreas, with special reference to colocalization of the four major islet hormones.

    abstract::Most, if not all, endocrine cells seem capable of synthesizing and storing more than one hormone. Such cellular colocalization of hormones can be due either to the presence of two or more specific granules within the cells or to colocalization of the hormones within a single granule. The present study was performed to...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Lukinius A,Ericsson JL,Grimelius L,Korsgren O

    更新日期:1992-10-01 00:00:00

  • Sgn1, a basic helix-loop-helix transcription factor delineates the salivary gland duct cell lineage in mice.

    abstract::The salivary system in mammals is comprised of three independently developed pairs of organs, the parotid, submaxillar, and sublingual glands. Each gland is composed of various ductal and acinar cell types that fulfill multiple roles. However, the molecular mechanisms regulating their biogenesis and functions are stil...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Yoshida S,Ohbo K,Takakura A,Takebayashi H,Okada T,Abe K,Nabeshima Y

    更新日期:2001-12-15 00:00:00

  • The representation of heart development in the gene ontology.

    abstract::An understanding of heart development is critical in any systems biology approach to cardiovascular disease. The interpretation of data generated from high-throughput technologies (such as microarray and proteomics) is also essential to this approach. However, characterizing the role of genes in the processes underlyi...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Khodiyar VK,Hill DP,Howe D,Berardini TZ,Tweedie S,Talmud PJ,Breckenridge R,Bhattarcharya S,Riley P,Scambler P,Lovering RC

    更新日期:2011-06-01 00:00:00

  • Directed Bmp4 expression in neural crest cells generates a genetic model for the rare human bony syngnathia birth defect.

    abstract::Congenital bony syngnathia, a rare but severe human birth defect, is characterized by bony fusion of the mandible to the maxilla. However, the genetic mechanisms underlying this birth defect are poorly understood, largely due to limitation of available animal models. Here we present evidence that transgenic expression...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: He F,Hu X,Xiong W,Li L,Lin L,Shen B,Yang L,Gu S,Zhang Y,Chen Y

    更新日期:2014-07-15 00:00:00

  • Strains matter: Success of murine in vitro spermatogenesis is dependent on genetic background.

    abstract::The current strategy to preserve fertility of male prepubertal cancer patients consists of cryopreservation of a testicular tissue biopsy containing spermatogonial stem cells (SSCs). While in humans, fertility restoration strategies from prepubertal testicular tissues are still under investigation and have not yet res...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Portela JMD,Mulder CL,van Daalen SKM,de Winter-Korver CM,Stukenborg JB,Repping S,van Pelt AMM

    更新日期:2019-12-01 00:00:00

  • Competition for factors and cellular resources as a principle of pattern formation in Hydra. II. Assistance of foot formation by heads and buds and a new model of pattern control.

    abstract::In Hydra long-range interactions between head, bud, and foot formation take place in addition to short-range interactions which are recognized as induction phenomena, occurring when transplants are placed into an environment of disparate positional value. Here the long-range promotion of foot formation is analyzed. Th...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Müller WA

    更新日期:1995-01-01 00:00:00

  • Inhibition of RHO-ROCK signaling enhances ICM and suppresses TE characteristics through activation of Hippo signaling in the mouse blastocyst.

    abstract::Specification of the trophectoderm (TE) and inner cell mass (ICM) lineages in the mouse blastocyst correlates with cell position, as TE derives from outer cells whereas ICM from inner cells. Differences in position are reflected by cell polarization and Hippo signaling. Only in outer cells, the apical-basal cell polar...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kono K,Tamashiro DA,Alarcon VB

    更新日期:2014-10-01 00:00:00

  • Directed overexpression of suppressor 2 of zeste and Posterior Sex Combs results in bristle abnormalities in Drosophila melanogaster.

    abstract::Three dominant second-chromosome rearrangement mutations in Drosophila melanogaster, Aristapedioid1 (Arp), vestigial-Depilate (vgD), and vestigial62 (vg62), result in developmental abnormalities of the bristle sense organs on the notum, abdomen, legs, and wing margin. The bristle abnormalities are associated with over...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Sharp EJ,Martin EC,Adler PN

    更新日期:1994-02-01 00:00:00

  • Quantitative analysis of cyclic AMP waves mediating aggregation in Dictyostelium discoideum.

    abstract::We have previously reported the detection of cAMP waves within monolayers of aggregating Dictyostelium discoideum cells (K. J. Tomchik and P.N. Devreotes, 1981, Science 212, 443-446). The computer-assisted analysis presented here of the fluorographic images of the cAMP waves reveals (1) all the waves have a consistent...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Devreotes PN,Potel MJ,MacKay SA

    更新日期:1983-04-01 00:00:00

  • fucosyltransferase1 and H-type complex carbohydrates modulate epithelial cell proliferation during prostatic branching morphogenesis.

    abstract::The prostate undergoes branching morphogenesis dependent on paracrine interactions between the prostatic epithelium and the urogenital mesenchyme. To identify cell-surface molecules that function in this process, monoclonal antibodies raised against epithelial cell-surface antigens were screened for antigen expression...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Marker PC,Stephan JP,Lee J,Bald L,Mather JP,Cunha GR

    更新日期:2001-05-01 00:00:00

  • Inhibition by dibutyryl cyclic AMP of the transition to metaphase of mouse oocyte nuclei and its reversal by cell fusion to metaphase oocytes.

    abstract::Mouse oocytes at metaphase I of meiotic maturation were treated with puromycin, which caused the condensed chromosomes to become decondensed to form an interphase nucleus. The chromosomes returned to a metaphase state 6.3 hr after the oocytes were transferred to puromycin-free medium [H. J. Clarke and Y. Masui (1983) ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Clarke HJ,Masui Y

    更新日期:1985-03-01 00:00:00

  • Mesodermal expression of integrin α5β1 regulates neural crest development and cardiovascular morphogenesis.

    abstract::Integrin α5-null embryos die in mid-gestation from severe defects in cardiovascular morphogenesis, which stem from defective development of the neural crest, heart and vasculature. To investigate the role of integrin α5β1 in cardiovascular development, we used the Mesp1(Cre) knock-in strain of mice to ablate integrin ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Liang D,Wang X,Mittal A,Dhiman S,Hou SY,Degenhardt K,Astrof S

    更新日期:2014-11-15 00:00:00

  • Distinct roles of Fgf8, Foxi1, Dlx3b and Pax8/2 during otic vesicle induction and maintenance in medaka.

    abstract::The development of the vertebrate inner ear is a complex process that has been investigated in several model organisms. In this work, we examined genetic interactions regulating early development of otic structures in medaka. We demonstrate that misexpression of Fgf8, Dlx3b and Foxi1 during early gastrulation is suffi...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Aghaallaei N,Bajoghli B,Czerny T

    更新日期:2007-07-15 00:00:00

  • Development of the enteric nervous system in the moth. I. Diversity of cell types and the embryonic expression of FMRFamide-related neuropeptides.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Copenhaver PF,Taghert PH

    更新日期:1989-01-01 00:00:00

  • Hand transcription factors cooperatively regulate development of the distal midline mesenchyme.

    abstract::Hand proteins are evolutionally conserved basic helix-loop-helix (bHLH) transcription factors implicated in development of neural crest-derived tissues, heart and limb. Hand1 is expressed in the distal (ventral) zone of the branchial arches, whereas the Hand2 expression domain extends ventrolaterally to occupy two-thi...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Barbosa AC,Funato N,Chapman S,McKee MD,Richardson JA,Olson EN,Yanagisawa H

    更新日期:2007-10-01 00:00:00

  • Inner cell allocation in the mouse morula: the role of oriented division during fourth cleavage.

    abstract::Two populations of blastomeres become positionally distinct during fourth cleavage in the mouse embryo; the inner cells become enclosed within the embryo and the outer cells form the enclosing layer. The segregation of these two cell populations is important for later development, because it represents the initial ste...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Sutherland AE,Speed TP,Calarco PG

    更新日期:1990-01-01 00:00:00

  • The coupling of cyclic GMP and photopolarization of Pelvetia zygotes.

    abstract::Unidirectional blue light directs the rhizoid-thallus axis in the apolar zygotes of Fucus and Pelvetia. Here, it is shown that blue light (but not red light) increased cyclic GMP levels of Pelvetia zygotes by about a factor of 2. When the increase in cyclic GMP was blocked by a guanylyl cyclase inhibitor, photopolariz...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Robinson KR,Miller BJ

    更新日期:1997-07-01 00:00:00

  • Ecdysone regulates morphogenesis and function of Malpighian tubules in Drosophila melanogaster through EcR-B2 isoform.

    abstract::Malpighian tubules are the osmoregulatory and detoxifying organs of Drosophila and its proper development is critical for the survival of the organism. They are made up of two major cell types, the ectodermal principal cells and mesodermal stellate cells. The principal and stellate cells are structurally and physiolog...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Gautam NK,Verma P,Tapadia MG

    更新日期:2015-02-15 00:00:00

  • The sea urchin kinome: a first look.

    abstract::This paper reports a preliminary in silico analysis of the sea urchin kinome. The predicted protein kinases in the sea urchin genome were identified, annotated and classified, according to both function and kinase domain taxonomy. The results show that the sea urchin kinome, consisting of 353 protein kinases, is close...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Bradham CA,Foltz KR,Beane WS,Arnone MI,Rizzo F,Coffman JA,Mushegian A,Goel M,Morales J,Geneviere AM,Lapraz F,Robertson AJ,Kelkar H,Loza-Coll M,Townley IK,Raisch M,Roux MM,Lepage T,Gache C,McClay DR,Manning G

    更新日期:2006-12-01 00:00:00

  • Multi-scale regulation of cell branching: Modeling morphogenesis.

    abstract::Plant growth and development are driven by extended phases of irreversible cell expansion generating cells that increase in volume from 10- to 100-fold. Some specialized cell types define cortical sites that reinitiate polarized growth and generate branched cell morphology. This structural specialization of individual...

    journal_title:Developmental biology

    pub_type: 杂志文章,评审


    authors: Li J,Kim T,Szymanski DB

    更新日期:2019-07-01 00:00:00

  • Roles of ADAM13-regulated Wnt activity in early Xenopus eye development.

    abstract::Pericellular proteolysis by ADAM family metalloproteinases has been widely implicated in cell signaling and development. We recently found that Xenopus ADAM13, an ADAM metalloproteinase, is required for activation of canonical Wnt signaling during cranial neural crest (CNC) induction by regulating a novel crosstalk be...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Wei S,Xu G,Bridges LC,Williams P,Nakayama T,Shah A,Grainger RM,White JM,DeSimone DW

    更新日期:2012-03-01 00:00:00

  • Induction of an epithelial-mesenchymal transition by an in vivo adheron-like complex.

    abstract::The embryonic vertebrate heart consists of two epithelia: the myocardium and endothelium, separated by the myocardial basement membrane (MBM). The myocardium has been shown to induce endothelial transformation into prevalvular mesenchyme in a temporally and site restricted manner. Previously, we hypothesized that the ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Mjaatvedt CH,Markwald RR

    更新日期:1989-11-01 00:00:00

  • Single-cell analysis of regulatory gene expression in quiescent and activated mouse skeletal muscle satellite cells.

    abstract::Repair and regeneration of adult skeletal muscle are mediated by satellite cells. In healthy muscle these rare mononucleate muscle precursor cells are mitotically quiescent. Upon muscle injury or degeneration, members of this self-renewing pool are activated to proliferate and then differentiate. Here we analyzed in s...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Cornelison DD,Wold BJ

    更新日期:1997-11-15 00:00:00

  • Sequential acquisition of transcriptional control during early embryonic development in the rabbit.

    abstract::Regulation of gene expression during early embryonic development in the rabbit was investigated by quantitative assay of firefly luciferase activity obtained by microinjection of three plasmid constructs using the regulatory region of polyomavirus promoter (PrPyV) with two different enhancer sequences (wild type or mu...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Christians E,Rao VH,Renard JP

    更新日期:1994-07-01 00:00:00

  • Stage-specific increase in cell surface galactosyltransferase activity during spermatogenesis in mice bearing t alleles.

    abstract::Mouse sperm surface galactosyltransferase (GalTase) mediates fertilization by binding to its appropriate glycoconjugate substrate in the egg zona pellucida. GalTase is present throughout all stages of spermatogenesis, during which time it redistributes within the plasma membrane from a uniform, diffuse distribution on...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Scully NF,Shur BD

    更新日期:1988-01-01 00:00:00

  • Constitutive activation of hedgehog signaling adversely affects epithelial cell fate during palatal fusion.

    abstract::Cleft palate is one of the most common craniofacial congenital defects in humans. It is associated with multiple genetic and environmental risk factors, including mutations in the genes encoding signaling molecules in the sonic hedgehog (Shh) pathway, which are risk factors for cleft palate in both humans and mice. Ho...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Li J,Yuan Y,He J,Feng J,Han X,Jing J,Ho TV,Xu J,Chai Y

    更新日期:2018-09-01 00:00:00

  • Inhibition of protein kinase A phenocopies ectopic expression of hedgehog in the CNS of wild-type and cyclops mutant embryos.

    abstract::The zebrafish hedgehog (hh) family members tiggy-winkle hedgehog (twhh) and sonic hedgehog (shh) are involved in patterning the ventral CNS and proximal eye. Using a dominant negative protein kinase A regulatory subunit mutant, we show that these hh activities are mediated by protein kinase A. The effects of dominant ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Ungar AR,Moon RT

    更新日期:1996-08-25 00:00:00

  • NFAT5/TonEBP controls early acquisition of notochord phenotypic markers, collagen composition, and sonic hedgehog signaling during mouse intervertebral disc embryogenesis.

    abstract::High osmolarity, bound water, and hydrostatic pressure contribute to notochord mechanics and its morphogenesis into the nucleus pulposus (NP) compartment of the intervertebral disc. Indeed, the osmoadaptive transcription factor, nuclear factor of activated T-cells 5 (NFAT5 aka TonEBP), is robustly expressed by residen...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Tessier S,Madhu V,Johnson ZI,Shapiro IM,Risbud MV

    更新日期:2019-11-15 00:00:00

  • Reception and transduction of the serotonin signal responsible for meiosis reinitiation in oocytes of the Japanese clam Ruditapes philippinarum.

    abstract::Prophase-arrested oocytes of Ruditapes philippinarum are triggered to undergo germinal vesicle breakdown under the influence of the neurohormone serotonin (5HT) and then arrest in metaphase 1. Our data show that these oocytes possess a single class of original 5HT receptors. Their binding parameters have been determin...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Gobet I,Durocher Y,Leclerc C,Moreau M,Guerrier P

    更新日期:1994-08-01 00:00:00

  • Natural size variation among embryos leads to the corresponding scaling in gene expression.

    abstract::Xenopus laevis frogs from laboratory stocks normally lay eggs exhibiting extensive size variability. We find that these initial size differences subsequently affect the size of the embryos prior to the onset of growth, and the size of tadpoles during the growth period. Even though these tadpoles differ in size, their ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Leibovich A,Edri T,Klein SL,Moody SA,Fainsod A

    更新日期:2020-06-15 00:00:00