Abstract:
:The msh/Msx family is a subclass of homeobox-containing genes suggested to perform a conserved function in the patterning of the early embryo. We had already isolated a member of this gene family (Ci-msxb) in Ciona intestinalis, which has a very complex expression pattern during embryogenesis. To identify the regulatory elements controlling its tissue-specific expression, we have characterized the gene structure and the regulatory upstream region. By electroporation experiments, we demonstrated that a 3.8-kb region located upstream of the gene contains all the regulatory elements able to reproduce its spatial expression pattern. Analyzing progressively truncated fragments of this region, three discrete and separate regions driving LacZ reporter gene expression in the ventral epidermis, primordial pharynx and neural territories have been identified. We further investigated the element(s) necessary for Ci-msxb activation in the nervous system during embryonic development by in vivo and in vitro experiments. Both electroporation and gel-shift assays of overlapping wild type and mutated oligonucleotides demonstrated that a unique sequence of 30 bp is involved in Ci-msxb neural activation from neurula to larva stage. This sequence contains consensus binding sites for various ubiquitous transcription factors such as TCF11 whose possible implication in formation of the regulatory complexes is discussed.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Russo MT,Donizetti A,Locascio A,D'Aniello S,Amoroso A,Aniello F,Fucci L,Branno Mdoi
10.1016/j.ydbio.2003.11.005subject
Has Abstractpub_date
2004-03-15 00:00:00pages
517-28issue
2eissn
0012-1606issn
1095-564Xpii
S001216060300695Xjournal_volume
267pub_type
杂志文章abstract::An immunocytochemical study of cholecalcin (28-kDa calcium-binding protein, CaBP, calbindin) was carried out during the development of the rat hippocampus. In normal animals, the protein appeared from Postnatal Day 3 in the granule cells of the dentate gyrus and from Day 5 in the CA1-CA2 pyramidal cells of Ammon's hor...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90474-x
更新日期:1987-11-01 00:00:00
abstract::In mammals, sex is determined in the bipotential embryonic gonad by a balanced network of gene actions which when altered causes disorders of sexual development (DSD, formerly known as intersex). In the XY gonad, presumptive Sertoli cells begin to differentiate when SRY up-regulates SOX9, which in turn activates FGF9 ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.11.010
更新日期:2008-02-01 00:00:00
abstract::The protein content of various size follicles was measured in Fundulus heteroclitus and indicated four phases of increase relative to follicle volume: Phases I (previtellogenic; estimated to be less than 0.01 mg/mm3), II (vitellogenic; 0.20 mg/mm3), III (early maturation; 0.03 mg/mm3), and IV (late maturation; 0 mg/mm...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90106-x
更新日期:1985-08-01 00:00:00
abstract::The nuclear receptor SpCOUP-TF is the highly conserved sea urchin homologue of the COUP family of transcription factors. Previous results from our laboratory demonstrated that SpCOUP-TF transcripts are localized in the egg and asymmetrically distributed in the early embryonic blastomeres (A. Vlahou et al., 1996, Devel...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9456
更新日期:2000-02-15 00:00:00
abstract::Using DiI fluorescent dextrans, we have created fate maps of the neural plate and early neural tube describing the extent of progenitor cell dispersal and the spatial origin of morphologically distinct neuronal cell types along the dorsoventral axis of the developing chick spinal cord. Nonuniform dispersal and mixing ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8912
更新日期:1998-07-01 00:00:00
abstract::The mature heart valves and septa are derived from the cardiac cushions which initially form as local outgrowths of mesenchymal cells within the outflow tract and atrioventricular regions. Endocardial cells respond to signals from the overlying myocardium and undergo an epithelial-to-mesenchymal transformation to inva...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0284
更新日期:2001-07-15 00:00:00
abstract::In vertebrates, hematopoietic and vascular progenitors develop from ventral mesoderm. The first primitive wave of hematopoiesis yields embryonic red blood cells, whereas progenitor cells of subsequent definitive waves form all hematopoietic cell lineages. In this report we examine the development of hematopoietic and ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8887
更新日期:1998-05-15 00:00:00
abstract::During heart formation, the heart grows and undergoes dramatic morphogenesis to achieve efficient embryonic function. Both in fish and amniotes, much of the growth occurring after initial heart tube formation arises from second heart field (SHF)-derived progenitor cell addition to the arterial pole, allowing chamber f...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.11.008
更新日期:2021-02-01 00:00:00
abstract::The C-X-C motif ligand 14 (CXCL14) is a recently discovered chemokine that is highly conserved in vertebrates and expressed in various embryonic and adult tissues. CXCL14 signaling has been implicated to function as an antiangiogenic and anticancer agent in adults. However, its function during development is unknown. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.01.008
更新日期:2017-03-01 00:00:00
abstract::Nociceptive neurons innervate the skin with complex dendritic arbors that respond to pain-evoking stimuli such as harsh mechanical force or extreme temperatures. Here we describe the structure and development of a model nociceptor, the PVD neuron of C. elegans, and identify transcription factors that control morphogen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.05.502
更新日期:2010-09-01 00:00:00
abstract::Symplastic spermatids (sys) male mice are sterile due to a recessive mutation that causes defective adhesion between spermatids and Sertoli cells within the seminiferous epithelium. We show that the mutation in sys mice involves a deletion of 1.24 Mb of chromosome 14. Comparative genomic analysis suggests that this re...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.06.054
更新日期:2006-10-15 00:00:00
abstract::A careful reanalysis of the literature indicates that the initial mechanism of activation in sea urchin eggs is remarkably similar to the mechanism established in medaka eggs: i.e., sea urchin eggs are activated by a qualitatively and quantitatively similar calcium explosion; one which is propagated in a wave sustaine...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/0012-1606(83)90276-2
更新日期:1983-10-01 00:00:00
abstract::Heparan sulfate proteoglycans are important modulators of growth factor signaling in a variety of patterning processes. Secreted growth factors that play critical roles in angiogenesis bind to heparan sulfate, and this association is affected by 6-O-sulfation of the heparan sulfate chains. Addition of 6-O-sulfate is c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.05.032
更新日期:2005-08-15 00:00:00
abstract::The morphogenetic processes responsible for the initial phase of gastrulation in sea urchin embryos are not known. Here we report observations of the size and position of clones of cells derived from horseradish peroxidase (HRP)-injected mesomeres and macromeres. The displacement of these clones during the initial pha...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90255-2
更新日期:1991-08-01 00:00:00
abstract::A polarity in gap junctional permeability normally exists in 32-cell stage Xenopus embryos, in that dorsal cells are relatively more coupled than ventral cells, as measured by transfer of Lucifer yellow dye. The current study extends our analysis of whether gap junctional permeability at this stage can be modulated by...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90227-8
更新日期:1992-05-01 00:00:00
abstract::The cortex and subcortical regions of the developing follicles and eggs of silkmoths are rich in cytoskeletal elements, particularly actin. In situ analysis using [3H]-polyuridylic acid and biotinylated oligo d(T) reveals a pattern of changes in poly(A)+ RNA distribution during oogenesis. The developing pattern of dis...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1031
更新日期:1993-02-01 00:00:00
abstract::We have examined the interfaces between protein and lipid sulfation, the response of progenitor cells to specific growth factors, and developmental lineage progression. The experimental system used is the oligodendrocyte (OL), the cell responsible for myelination of axons in the central nervous system. The ability to ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1105
更新日期:1994-04-01 00:00:00
abstract::nautilus (nau), one member of the myogenic regulatory family of bHLH-encoding genes, is expressed in a subset of muscle precursors and differentiated fibers in the Drosophila embryo. To elucidate the role of nautilus in myogenesis, we have misexpressed it using the GAL4-targeted system. We find that ectopic expression...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.8434
更新日期:1997-01-15 00:00:00
abstract::In the developing heart, the epicardium is essential for coronary vasculogenesis as it provides precursor cells that become coronary vascular smooth muscle and perivascular fibroblasts. These precursor cells are derived from the epicardium via epithelial-mesenchymal transformation (EMT). The factors that regulate epic...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0254
更新日期:2001-06-01 00:00:00
abstract::In the mouse embryo, the onset of zygotic transcription occurs at the end of the first cell cycle, upon completion of DNA replication. We show that the nonhistone chromosomal protein HMG-I, whose translocation into the pronuclei of one-cell embryos is linked to this first round of DNA synthesis, plays a critical role ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9668
更新日期:2000-05-15 00:00:00
abstract::teashirt (tsh) encodes a zinc-finger protein that is thought to be part of a network that contributes to regionalization of the Drosophila embryo and establishes the domains of Hox protein function. tsh and the Hox gene Sex combs reduced (Scr) are essential to establish the identity of the first thoracic segment. We u...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.04.028
更新日期:2007-07-01 00:00:00
abstract::The role of the wound epithelium in amphibian limb regeneration is not understood. We showed previously that monoclonal antibody (mAb) WE3 stains the wound epithelium but not skin epidermis, suggesting that the WE3 antigen may be a marker for, or be important in, the function of the wound epithelium. In the present st...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90188-7
更新日期:1989-10-01 00:00:00
abstract::Two distinct DNA ligases are expressed during early sea urchin embryogenesis. A light form (50 kDa) is found in unfertilized eggs (oocyte form) and a heavier enzyme (110 kDa) is observed at the two-cell stage (embryonic form). The chronology of the change reveals that the embryonic form is detected 90 min after fertil...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90479-9
更新日期:1987-11-01 00:00:00
abstract::Receptor protein tyrosine phosphatases (RPTPs) have been shown to play key roles in regulating axon guidance and synaptogenesis. HmLAR2, one of two closely related LAR-like RPTPs in the embryonic leech, is expressed in a few central neurons and in a unique segmentally-iterated peripheral cell, the comb cell (CC). Here...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.05.522
更新日期:2008-08-01 00:00:00
abstract::The Ci-Dll-B gene is an early regulator of ectodermal development in the ascidian Ciona intestinalis (Imai et al., 2006). Ci-Dll-B is located in a convergently transcribed bigene cluster with a tandem duplicate, Ci-Dll-A. This clustered genomic arrangement is the same as those of the homologous vertebrate Dlx genes, w...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.02.009
更新日期:2011-05-15 00:00:00
abstract::The mammalian prostate arises from the urogenital sinus and few factors have been identified to be important in the early stages of prostate development. In this study we show that the transcription factor Sox9 is expressed in the epithelia of all mouse prostatic lobes from the initial stages of their development. We ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.01.030
更新日期:2008-04-15 00:00:00
abstract::The sensory neurons of the Drosophila wing differentiate during the initial stages of metamorphosis, appearing in the imaginal wing disc as it everts and flattens. These identifiable neurons arise in a stereotyped sequence, and lay down a specific pattern of axon bundles which travel proximally to the CNS. In several ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90044-2
更新日期:1985-04-01 00:00:00
abstract::A modification of the scrape-loading/dye transfer technique was used to study gap junctional communication along the anterior-posterior (A-P) axis of embryonic chick wing buds at an early stage of development (stage 20/21) when positional values along the A-P axis are being specified. Extensive intercellular transfer ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90271-4
更新日期:1991-12-01 00:00:00
abstract::To examine the role of postsynaptic activity in regulating the rate of neuromuscular synapse elimination, contractile activity of neonatal rabbit soleus muscles was decreased by chronic superfusion of alpha-bungarotoxin (alpha-BGT) over their surfaces. Superfusion was begun at 6 days postnatal and continued for a vari...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80009-0
更新日期:1989-02-01 00:00:00
abstract::Previous attempts to study sorting out of Drosophila imaginal disc cells have been hampered by an inability to thoroughly dissociate these cells and the need to use cuticular markers which require several days of in vivo culture. This study overcomes these limitations by using a new dissociation procedure and a geneti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90049-1
更新日期:1985-04-01 00:00:00