Abstract：
:Receptor protein tyrosine phosphatases (RPTPs) have been shown to play key roles in regulating axon guidance and synaptogenesis. HmLAR2, one of two closely related LAR-like RPTPs in the embryonic leech, is expressed in a few central neurons and in a unique segmentally-iterated peripheral cell, the comb cell (CC). Here we show that tagged HmLAR2-EGFP has a punctate pattern of expression in the growth cones of the CC, particularly at the tips of extending filopodia. Moreover, although expression of the wild-type EGFP-tagged receptor does not affect CC growth cone morphology, expression of a putative dominant-negative mutant of the receptor, CS-HmLAR2, leads to the enlargement of the growth cones, a shortening of filopodia, and errors in cellular tiling. RNAi of several candidate substrate signaling proteins, Lena (leech Ena/Vasp), beta-integrin and paxillin, but not beta-catenin, phenocopies particular aspects of the effects of HmLAR2 RNAi. For paxillin, which co-localizes with HmLAR2 at growth cone puncta, knock-down led to a reduction in the number of such puncta. Together, our data suggests that HmLAR2 regulates the morphology of the growth cone by controlling F-actin polymerization and focal adhesion complexes.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Baker MW,Peterson SM,Macagno ERdoi
10.1016/j.ydbio.2008.05.522subject
Has Abstractpub_date
2008-08-01 00:00:00pages
215-25issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(08)00871-3journal_volume
320pub_type
杂志文章abstract：:Eye cups from stage 14-28 (E2 to E5) chick and quail embryos consisting of neural retina, lens, and vitreous body were cultured for 1 or 2 days. These eyes expanded by proliferation of the retinal cells and the surface areas of the retinae increased several-fold. The area covered by ganglion cells and axons also expan...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90194-6
更新日期：1986-04-01 00:00:00
abstract：:We wish to understand how organ-specific structures assemble during embryonic development. In the present paper, we consider what determines the subapical position of the terminal web in the intestinal cells of the nematode Caenorhabditis elegans. The terminal web refers to the organelle-depleted, intermediate filamen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.003
更新日期：2004-04-15 00:00:00
abstract：:Carbohydrates have been shown to mediate or modulate a number of important events in the development of the nervous system; however, there is little evidence that they participate directly in the development of synapses. One carbohydrate structure that is likely to be important in synaptic development of the neuromusc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0530
更新日期：2002-02-01 00:00:00
abstract：:The msh/Msx family is a subclass of homeobox-containing genes suggested to perform a conserved function in the patterning of the early embryo. We had already isolated a member of this gene family (Ci-msxb) in Ciona intestinalis, which has a very complex expression pattern during embryogenesis. To identify the regulato...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.11.005
更新日期：2004-03-15 00:00:00
abstract：:In Xenopus gastrulation, the involuting mesodermal and non-involuting ectodermal cells remain separated from each other, undergoing convergent extension. Here, we show that Eph-ephrin signaling is crucial for the tissue separation and convergence during gastrulation. The loss of EphA4 function results in aberrant gast...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.12.012
更新日期：2011-02-15 00:00:00
abstract：:Lens growth involves the proliferation of epithelial cells, followed by their migration to the equator region and differentiation into secondary fiber cells. It is widely accepted that fibroblast growth factor (FGF) signaling is required for the differentiation of lens epithelial cells into crystallin-rich fibers, but...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.05.029
更新日期：2012-08-01 00:00:00
abstract：:A gene regulatory network subcircuit comprising the otx, wnt8, and blimp1 genes accounts for a moving torus of gene expression that sweeps concentrically across the vegetal domain of the sea urchin embryo. Here we confirm by mutation the inputs into the blimp1cis-regulatory module predicted by network analysis. Its es...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.10.042
更新日期：2008-01-15 00:00:00
abstract：:Nucleostemin (NS), a member of a family of nucleolar GTP-binding proteins, is highly expressed in proliferating cells such as stem and cancer cells and is involved in the control of cell cycle progression. Both depletion and overexpression of NS result in stabilization of the tumor suppressor p53 protein in vitro. Alt...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.04.028
更新日期：2011-07-15 00:00:00
abstract：:NK-2-class homeobox genes have been identified in a variety of metazoans, from sponges to arthropods and vertebrates, and have been shown to play roles in a variety of cell and tissue specifications. Here we describe the characterization of the NK-2 homolog CnNK-2 from Hydra vulgaris, a freshwater cnidarian. CnNK-2 ex...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0321
更新日期：1996-12-15 00:00:00
abstract：:The nuclear lamina is an extensive protein network that underlies the inner nuclear envelope. This network includes the LAP2-emerin-MAN1-domain (LEM-D) protein family, proteins that share an association with the chromatin binding protein Barrier-to-autointegration factor (BAF). Loss of individual LEM-D proteins causes...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.05.001
更新日期：2016-07-01 00:00:00
abstract：:Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90275-0
更新日期：1983-09-01 00:00:00
abstract：:The fertilization potential in Phallusia mammillata consisted of an initial rapid depolarization. This initial sperm-triggered depolarization was followed by a phase of membrane depolarization which was of either long or short duration, depending on the eggs. When of long duration, the phase of membrane depolarization...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90108-s
更新日期：1992-10-01 00:00:00
abstract：:A mammalian embryo experiences the first cell segregation at the blastocyst stage, in which cells giving form to the embryo are sorted into two lineages; trophectoderm (TE) and inner cell mass (ICM). This first cell segregation process is governed by cell position-dependent Hippo signaling, which is a phosphorylation ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.09.004
更新日期：2020-12-01 00:00:00
abstract：:Connexin 43 (Cx43) knockout mice and transgenic mice (CMV43) overexpressing the Cx43 gap junction gene exhibit heart defects involving the conotruncus and right ventricle. Based on the heart phenotype and Cx43 gene and transgene expression pattern, we previously proposed that the heart defects may reflect a role for g...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8891
更新日期：1998-06-01 00:00:00
abstract：:In mouse chimeras with parthenogenetic cell contribution, the skeletal musculature appears to be largely devoid of parthenogenetically derived cells. To analyze the appearance and early distribution of myotomal cells in parthenotes, we determined the expression of the muscle-specific transcription factors myogenin, MY...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1004
更新日期：1994-01-01 00:00:00
abstract：:Biomineralization, the biologically controlled formation of mineral deposits, is of widespread importance in biology, medicine, and engineering. Mineralized structures are found in most metazoan phyla and often have supportive, protective, or feeding functions. Among deuterostomes, only echinoderms and vertebrates pro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.07.047
更新日期：2006-12-01 00:00:00
abstract：:I report on the synthesis of fibronectin in the developing chick Müllerian duct mesenchymal cells. Before the differentiation of female chick Müllerian duct, the amount of fibronectin in the cells of the right duct is 44% lower than in the left duct. While after differentiation, the amount of fibronectin in the right ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90047-m
更新日期：1990-07-01 00:00:00
abstract：:The dysgenetic lens (dyl) mouse mutant has mutations in Foxe3, which inactivate DNA binding by the encoded forkhead transcription factor. Here we confirm, by targeted inactivation, that Foxe3 mutations are responsible for the dyl phenotype, which include loss of lens epithelium; a small, cataractic lens; and failure o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.09.021
更新日期：2007-02-01 00:00:00
abstract：:The accumulation of the mRNAs from four Strongylocentrotus purpuratus actin genes (the single muscle gene M, and three cytoskeletal genes CyI, CyIIIa, and CyIIIb) and of transcripts from an RNA polymerase III-transcribed repeated sequence family (SURF1) was followed throughout the early development of hybrid embryos o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90439-3
更新日期：1988-11-01 00:00:00
abstract：:Activin is a potent inducer of axial mesoderm in vitro and may have a similar role in vivo. Xenopus laevis eggs contain significant amounts of activin or activin-like factors, but maternal activin transcripts have not been detected in Xenopus eggs. The maternal activin protein might be translated from activin beta A o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1265
更新日期：1993-10-01 00:00:00
abstract：:A pair of the Drosophila eye-antennal disc gives rise to four distinct organs (eyes, antennae, maxillary palps, and ocelli) and surrounding head cuticle. Developmental processes of this imaginal disc provide an excellent model system to study the mechanism of regional specification and subsequent organogenesis. The do...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.06.015
更新日期：2011-08-15 00:00:00
abstract：:Metamorphosis is a postembryonic developmental process that involves morphophysiological and behavioral changes, allowing organisms to adapt into a novel environment. In some amphibians, aquatic organisms undergo metamorphosis to adapt in a terrestrial environment. In this process, these organisms experience major cha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.08.002
更新日期：2020-10-01 00:00:00
abstract：:Immunocytochemical studies were performed to determine if denervated rodent iris produces nerve growth factor (NGF) in a form chemically similar to that of the 7S NGF complex in mouse submandibular glands. Antisera to the alpha, beta, and gamma subunits of 7S NGF were raised in rabbits and characterized on immunoblots...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90201-0
更新日期：1986-04-01 00:00:00
abstract：:During metamorphosis of insect epithelial monolayers, cells die, divide, and rearrange. In Drosophila undifferentiated diploid cells destined to form the adult cuticle of each abdominal segment segregate early in development from the surrounding polyploid larval epithelial cells of that segment as eight groups of dipl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.03.017
更新日期：2018-06-01 00:00:00
abstract：:The transcription factors GATA4, GATA5 and GATA6 are important regulators of heart muscle differentiation (cardiomyogenesis), which function in a partially redundant manner. We identified genes specifically regulated by individual cardiogenic GATA factors in a genome-wide transcriptomics analysis. The genes regulated ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.11.017
更新日期：2018-02-01 00:00:00
abstract：:To understand cell interactions during induction of skin appendages, we studied the roles of adhesion molecules N-CAM, tenascin, integrin, and fibronectin during feather development. Tenascin appeared in a periodic pattern on epithelia and was so far the earliest molecule detected in placodes. Three placode domains we...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90009-6
更新日期：1992-03-01 00:00:00
abstract：:Antibodies to nonerythroid alpha spectrin (p 230) were used to study the distribution of this polypeptide in mouse germ cells, zygote, and early embryonic cells. In the primordial germ cells, fetal oocytes, and spermatogonia, spectrin was found predominantly in the form of a narrow condensed subplasmalemmal band, as i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90389-1
更新日期：1986-03-01 00:00:00
abstract：:In the course of embryogenesis multicellular structures and organs are assembled from constituent cells. One structural component common to many organs is the tube, which consists most simply of a luminal space surrounded by a single layer of epithelial cells. The notochord of ascidian Ciona forms a tube consisting of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.03.015
更新日期：2009-06-15 00:00:00
abstract：:In higher organisms, mononucleated myoblasts fuse to form multinucleated myotubes. During this process, myoblasts undergo specific changes in cell morphology and cytoarchitecture. Previously, we have shown that the actin regulator Kette (Hem-2/Nap-1) is essential for myoblast fusion. In this study, we describe the rol...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.01.015
更新日期：2007-04-15 00:00:00
abstract：:It has long been appreciated that the localization of cytoplasmic determinants in the egg can provide the foundation for patterning in the embryo. Differences in cell fate among the early blastomeres are thus a consequence of asymmetric distributions of informational molecules prior to fertilization. The frog egg has ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8773
更新日期：1997-12-15 00:00:00
