Abstract：
:It has long been appreciated that the localization of cytoplasmic determinants in the egg can provide the foundation for patterning in the embryo. Differences in cell fate among the early blastomeres are thus a consequence of asymmetric distributions of informational molecules prior to fertilization. The frog egg has a single axis of asymmetry present prior to fertilization, the animal/vegetal axis, and the localization of developmental information appears to be polarized along this axis. Such developmental information can be localized as either RNA or protein; localized RNAs are well documented in the Xenopus oocyte, and some are thought to play roles in axial patterning. While it is apparent that not all of the localized maternal components are RNAs, much less is known about maternal proteins that might be localized in the egg. In the present study, we have taken a novel approach to identify localized maternal proteins within the Xenopus egg. Using a subtractive immunization strategy, we have generated monoclonal antibodies which recognize antigens that are restricted to the vegetal cortex of fertilized eggs. Analysis of biogenesis during oogenesis reveals two distinct patterns of localization to the cortex. At least three of these localized antigens are proteins, and these localized proteins could represent maternal determinants with roles in patterning.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Denegre JM,Ludwig ER,Mowry KLdoi
10.1006/dbio.1997.8773subject
Has Abstractpub_date
1997-12-15 00:00:00pages
446-54issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(97)98773-Xjournal_volume
192pub_type
杂志文章abstract：:Through the course of evolution, the gastrointestinal (GI) tract has been modified to maximize nutrient absorption, forming specialized segments that are morphologically and functionally distinct. Here we show that the GI tract of the Mexican tetra, Astyanax mexicanus, has distinct regions, exhibiting differences in m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.06.004
更新日期：2018-09-15 00:00:00
abstract：:Normal animal development requires accurate cell divisions, not only in the early stages of rapid embryonic cleavages, but also in later developmental stages. The Caenorhabditis elegans unc-85 gene is implicated only in cell divisions that occur post-embryonically, primarily in terminal neuronal lineages. Variable pos...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.013
更新日期：2008-07-01 00:00:00
abstract：:To analyse cell migration and the differentiation potential of migratory stem cells in Hydractinia, we generated animals with an eGFP reporter gene stably expressed and transmitted via the germline. The transgene was placed under the control of two different actin promoters and the promoter of elongation factor-1α. On...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.08.017
更新日期：2010-12-01 00:00:00
abstract：:Hes3 belongs to the Hes basic helix-loop-helix family of transcriptional repressors that play central roles in maintaining progenitor cells and regulating binary cell fate decisions in the embryo. During Xenopus laevis development, hes3 is expressed in the embryonic ectoderm in a horseshoe shape domain at the edge of ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.07.011
更新日期：2018-10-01 00:00:00
abstract：:The extracellular matrix of Xenopus laevis oocytes was analyzed before and after meiotic maturation using quick-freeze, deep-etch, rotary-shadow electron microscopy. The perivitelline space (PS) of the meiotically immature oocyte contains a filamentous network which connects microvilli (MV) and follicle cell macrovill...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(89)80044-2
更新日期：1989-01-01 00:00:00
abstract：:The experimental manipulation of early embryologic events, resulting in the misexpression of the homeobox transcription factor pitx2, is associated with subsequent defects of laterality in a number of vertebrate systems. To clarify the role of one pitx2 isoform, pitx2c, in determining the left-right axis of amphibian ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00389-0
更新日期：2003-10-15 00:00:00
abstract：:The eyes absent (eya) gene plays an essential role in the events that lead to formation of the Drosophila eye; without expression of eya in retinal progenitor cells, they undergo programmed cell death just prior to the morphogenetic furrow, leading to an eyeless or reduced eye phenotype. The eya gene has recently been...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8845
更新日期：1998-04-01 00:00:00
abstract：:The primary lung bud originates from the foregut and develops into the bronchial tree by repetitive branching and outgrowing of the airway. The Sry related HMG box protein Sox2 is expressed in a cyclic manner during initiation and branching morphogenesis of the lung. It is highly expressed in non-branching regions and...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.02.035
更新日期：2008-05-01 00:00:00
abstract：:Animal development is controlled by gene regulation networks that are composed of sequence-specific transcription factors (TF) and cell signaling molecules (ST). Although housekeeping genes have been reported to show clustering in the animal genomes, whether the genes comprising a given regulatory network are physical...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.01.009
更新日期：2008-04-15 00:00:00
abstract：:Intestinal tract development is a coordinated process involving signaling among the progenitors and developing cells from all three germ layers. Development of endoderm-derived intestinal epithelium has been shown to depend on epigenetic modifications, but whether that is also the case for intestinal tract cell types ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.08.002
更新日期：2019-11-15 00:00:00
abstract：:In ascidian embryos, the earliest transcription from the zygotic genome begins between the 8-cell and 16-cell stages. Gata.a, a maternally expressed Gata transcription factor, activates target genes specifically in the animal hemisphere, whereas the complex of β-catenin and Tcf7 antagonizes the activity of Gata.a and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.11.009
更新日期：2020-02-15 00:00:00
abstract：:In the retinotectal projection, the Eph receptor tyrosine kinase ligands ephrinA2 and ephrinA5 are differentially expressed not only in the tectum, but also in a high-nasal-to-low-temporal pattern in the retina. Recently, we have shown that retrovirally driven overexpression of ephrinA2 on retinal axons leads to topog...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9489
更新日期：1999-12-01 00:00:00
abstract：:Newts regenerate lost limbs through a complex process involving dedifferentiation, migration, proliferation, and redifferentiation of cells proximal to the amputation plane. To identify the genes controlling these cellular events, we performed a differential display analysis between regenerating and nonregenerating li...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.12.003
更新日期：2005-03-01 00:00:00
abstract：:Exocytosis of cortical granules in mouse eggs is required to produce the zona pellucida block to polyspermy. In this study, we examined the role of microfilaments and microtubules in the regulation of cortical granule movement toward the cortex during oocyte maturation and anchoring of cortical granules in the cortex....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8945
更新日期：1998-08-01 00:00:00
abstract：:Despite undergoing normal development and acquiring normal morphology and motility, mutations in spe-38 or trp-3/spe-41 cause identical phenotypes in Caenorhabditis elegans-mutant sperm fail to fertilize oocytes despite direct contact. SPE-38 is a novel, four-pass transmembrane protein and TRP-3/SPE-41 is a Ca(2+)-per...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.02.037
更新日期：2012-05-15 00:00:00
abstract：:We recently demonstrated a critical role for two-pore channel type 2 (TPC2)-mediated Ca2+ release during the differentiation of slow (skeletal) muscle cells (SMC) in intact zebrafish embryos, via the introduction of a translational-blocking morpholino antisense oligonucleotide (MO). Here, we extend our study and demon...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.03.031
更新日期：2017-05-15 00:00:00
abstract：:Tie1 is a receptor tyrosine kinase expressed in endothelial cells, where it modulates Angiopoietin/Tie2 signaling. Previous studies have shown that mouse Tie1 mutants exhibit severe cardiovascular defects; however, much remains to be learned about the role of Tie1, especially during cardiac development. To further und...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.09.008
更新日期：2021-01-01 00:00:00
abstract：:Extensive elongation of lens fiber cells is a central feature of lens morphogenesis. Our study investigates the role of N-cadherin junctions in this process in vivo. We investigate both the molecular players involved in N-cadherin junctional maturation and the subsequent function of these junctions as epicenters for t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.10.009
更新日期：2011-01-15 00:00:00
abstract：:Zygote arrest (Zar) proteins are crucial for early embryonic development, but their molecular mechanism of action is unknown. The Translational Control Sequence (TCS) in the 3' untranslated region (UTR) of the maternal mRNA, Wee1, mediates translational repression in immature Xenopus oocytes and translational activati...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.06.012
更新日期：2012-09-15 00:00:00
abstract：:The definitive feature of the many thousand cis-regulatory control modules in an animal genome is their information processing capability. These modules are "wired" together in large networks that control major processes such as development; they constitute "genomic computers." Each control module receives multiple in...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2007.08.009
更新日期：2007-10-15 00:00:00
abstract：:In Drosophila, wing hairs are aligned in a distally oriented, parallel array. The frizzled pathway determines proximal-distal cell polarity in the wing; however, in frizzled pathway mutants, wing hairs remain parallel. How wing hairs align has not been determined. We have demonstrated a novel role for the septate junc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.07.040
更新日期：2004-11-15 00:00:00
abstract：:We studied the pathway of 5S ribosomal RNA (rRNA) during oogenesis in Xenopus from its storage in the cytoplasm to incorporation into ribosomes in the nucleus. Ribonucleoprotein particle (RNP) assembly assays reveal striking differences in the behavior of oocyte-type and somatic-type 5S rRNA after microinjection into ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1080
更新日期：1995-04-01 00:00:00
abstract：:Fertilization triggers activation of Src-family kinases in eggs of various species including marine invertebrates and lower vertebrates. While immunofluorescence studies have localized Src-family kinases to the plasma membrane or cortical cytoplasm, no information is available regarding the extent to which these kinas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.03.041
更新日期：2006-07-15 00:00:00
abstract：:Using gene-specific probes derived from four mouse gamma-crystallin cDNAs, we have examined the regulation of different members of the mouse gamma-crystallin gene family during lens development. Our analysis revealed that, while the different gamma-crystallin genes appear to be coordinately activated during embryogene...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90227-2
更新日期：1987-01-01 00:00:00
abstract：:Oligodendrocytes are produced from the same region of the ventral spinal cord that earlier generated motor neurons in bird and rodent embryos. Motor neuron and oligodendrocyte precursor cells express Olig genes, which encode basic helix-loop-helix transcription factors that play important roles in the development of b...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0738
更新日期：2002-08-15 00:00:00
abstract：:Vasa is a DEAD-box RNA helicase that functions in translational regulation of specific mRNAs. In many animals it is essential for germ line development and may have a more general stem cell role. Here we identify vasa in two sea urchin species and analyze the regulation of its expression. We find that vasa protein acc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.11.039
更新日期：2008-02-15 00:00:00
abstract：:To investigate the spatiotemporal dynamics of skin pattern formation, we developed a simple method for artificially disarranging the placement of all three pigment cell types in the body trunk of zebrafish (Danio rerio). We generated transgenic fish with melanophores that ectopically expressed a variant of channelrhod...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.12.019
更新日期：2020-04-01 00:00:00
abstract：:Myogenesis in the embryo and the adult mammal consists of a highly organized and regulated sequence of cellular processes to form or repair muscle tissue that include cell proliferation, migration, and differentiation. Data from cell culture and in vivo experiments implicate both FGFs and HGF as critical regulators of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0416
更新日期：2001-11-01 00:00:00
abstract：:We have investigated the accumulation and adenylation of the maternal mRNA during oogenesis in the oocytes of the marine worm Urechis caupo. The analysis, using in vitro translation and cDNA probes to assay for specific mRNAs, demonstrates that different maternal mRNAs accumulate with different patterns during oogenes...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90347-7
更新日期：1986-09-01 00:00:00
abstract：:Chick sensory neurons grow to their correct targets in the hindlimb from the outset during normal development and following various experimental manipulations. This may result not because sensory neurons respond to specific limb-derived cues, but because they interact in some way with motoneurons which are responsive ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90023-0
更新日期：1986-12-01 00:00:00
