Abstract:
:Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Mitchison TJ,Sedat Jdoi
10.1016/0012-1606(83)90275-0subject
Has Abstractpub_date
1983-09-01 00:00:00pages
261-4issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(83)90275-0journal_volume
99pub_type
杂志文章abstract::The current strategy to preserve fertility of male prepubertal cancer patients consists of cryopreservation of a testicular tissue biopsy containing spermatogonial stem cells (SSCs). While in humans, fertility restoration strategies from prepubertal testicular tissues are still under investigation and have not yet res...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.08.007
更新日期:2019-12-01 00:00:00
abstract::We demonstrate that interspecific sequence conservation can provide a systematic guide to the identification of functional cis-regulatory elements within a large expanse of genomic DNA. The test was carried out on the otx gene of Strongylocentrotus purpuratus. This gene plays a major role in the gene regulatory networ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0618
更新日期:2002-06-01 00:00:00
abstract::Adhesive extracellular matrix (ECM) molecules appear to play roles in the migration of neural crest cells, and may also provide cues for differentiation of these cells into a variety of phenotypes. We are studying the influences of specific ECM components on crest differentiation at the levels of both individual cells...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90112-v
更新日期:1990-09-01 00:00:00
abstract::To understand mechanisms of sex determination, it is important to know the lineage relationships of cells comprising the gonads. For example, in mice, the Y-linked gene Sry triggers differentiation of Sertoli cells from a cell population originating in the coelomic epithelium overlying the nascent gonad that also give...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.09.007
更新日期:2007-02-01 00:00:00
abstract::Differentiation from a haploid round spermatid to a highly streamlined, motile sperm requires temporal and spatial regulation of the expression of numerous proteins. One form of regulation is the storage of translationally repressed mRNAs. In Drosophila spermatocytes, the transcription of many of these translationally...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.02.018
更新日期:2013-05-01 00:00:00
abstract::Heart valve structures derived from mesenchymal cells of the endocardial cushions (ECs) are composed of highly organized cell lineages and extracellular matrix. Sox9 is a transcription factor required for both early and late stages of cartilage formation that is also expressed in the developing valves of the heart. Th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.02.002
更新日期:2007-05-01 00:00:00
abstract::Mouse sperm surface galactosyltransferase (GalTase) mediates fertilization by binding to its appropriate glycoconjugate substrate in the egg zona pellucida. GalTase is present throughout all stages of spermatogenesis, during which time it redistributes within the plasma membrane from a uniform, diffuse distribution on...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90072-3
更新日期:1988-01-01 00:00:00
abstract::Pax3 is a transcription factor expressed in somitic mesoderm, dorsal neural tube and pre-migratory neural crest during embryonic development. We have previously identified cis-acting enhancer elements within the proximal upstream genomic region of Pax3 that are sufficient to direct functional expression of Pax3 in neu...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.12.030
更新日期:2010-03-15 00:00:00
abstract::Directed cell movement is integral to both embryogenesis and hematopoiesis. In the adult, the chemokine family of secreted proteins signals migration of hematopoietic cells through G-coupled chemokine receptors. We detected embryonic expression of chemokine receptor messages by RT-PCR with degenerate primers at embryo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9405
更新日期:1999-09-15 00:00:00
abstract::Xenopus laevis is among the few species that are capable of fully regenerating a lost lens de novo. This occurs upon removal of the lens, when secreted factors from the retina are permitted to reach the cornea epithelium and trigger it to form a new lens. Although many studies have investigated the retinal factors tha...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.12.036
更新日期:2014-02-15 00:00:00
abstract::Although mitogen-activated protein kinase (MAPK) is a well-known cell cycle regulator, emerging studies have also implicated its activity in the regulation of intracellular calcium concentration ([Ca2+](i)) and secretion. Those studies raise the hypothesis that MAPK activity during oocyte maturation and early fertiliz...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.03.029
更新日期:2007-06-15 00:00:00
abstract::The four members of the albumin gene family encode the serum transport proteins albumin, alpha-fetoprotein, alpha-albumin, and vitamin D-binding protein. These genes are transcribed primarily in the liver with each having a different pattern of developmental expression. The tight linkage of these genes, particularly t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.09.026
更新日期:2009-12-15 00:00:00
abstract::Midline defects account for approximately 5% of congenital abnormalities observed at birth. However, the molecular mechanisms underlying the formation of the ventral body wall are not well understood. Recent studies linked mutations in Porcupine-an O-acetyl transferase mediating Wnt ligand acylation-with defects in th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.02.014
更新日期:2015-05-15 00:00:00
abstract::Although skeletal muscles appear superficially alike at different anatomical locations, in reality there is considerably more diversity than previously anticipated. Heterogeneity is not only restricted to completely developed fibers, but is clearly apparent during development at the molecular, cellular and anatomical ...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2007.06.006
更新日期:2007-08-15 00:00:00
abstract::Cadherin-mediated cell-cell interactions are thought to be critical in controlling cell sorting during embryogenesis. Here, we report that chimeric embryos generated with N-cadherin-deficient (N-cadherin(-/-)) embryonic stem cells develop further than embryos completely lacking N-cadherin only when the myocardium cons...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0250
更新日期:2001-06-01 00:00:00
abstract::Monoclonal antibodies have been generated to determine the fate of sperm-specific surface components of the sea urchin, Arbacia punctulata, subsequent to gamete fusion. Monoclonal antibody-7 (MAB-7) reacted with two polypeptide bands having apparent molecular masses of 33 and 35 kDa derived from the sperm plasma membr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80036-3
更新日期:1989-01-01 00:00:00
abstract::Gene transcription mediates many vital aspects of mammalian embryonic development. A comprehensive characterization and analysis of the dynamics of gene transcription in the embryo is therefore likely to provide significant insights into the basic mechanisms of this process. We used microarrays to map transcription in...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.09.036
更新日期:2005-12-15 00:00:00
abstract::The molting cycle in insects is regulated by the fluctuating levels of ecdysteroids. Twenty-four hours prior to the pupal molt in Manduca sexta steroid titers are relatively high and they fall to very low levels at 4 hr before the molt. Several physiological events accompanying molts have been shown to require the ris...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1115
更新日期:1994-04-01 00:00:00
abstract::Caudal homeobox genes are found across animals, typically linked to two other homeobox genes in what has been called the ParaHox cluster. These genes have been proposed to pattern the anterior-posterior axis of the endoderm ancestrally, but the expression of Caudal in extant groups is varied and often occurs in other ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.10.010
更新日期:2021-02-01 00:00:00
abstract::Complex intrinsic and extrinsic mechanisms determine neural cell fate during development of the nervous system. Using Id4 deficient mice, we show that Id4 is required for normal development of the central nervous system (CNS), timing neural differentiation in the developing forebrain. In the absence of Id4, the ventri...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.02.001
更新日期:2005-04-15 00:00:00
abstract::In medusae of the hydrozoan Cytaeis uchidae, oocyte meiotic maturation and spawning occur as a consequence of dark-light transition. In this study, we investigated the mechanism underlying the initiation of meiotic maturation using in vitro (isolated oocytes from ovaries) and in vivo (ovarian oocytes in medusae) syste...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.06.034
更新日期:2006-10-01 00:00:00
abstract::Integrins are alphabeta transmembrane receptors that function in key cellular processes, including cell adhesion, differentiation, and extracellular matrix deposition through interactions with extracellular, membrane, and cytoplasmic proteins. We previously identified and cloned a muscle beta1 integrin cytoplasmic bin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00304-x
更新日期:2003-09-01 00:00:00
abstract::We previously demonstrated that male mice deficient in the soluble adenylyl cyclase (sAC) are sterile and produce spermatozoa with deficits in progressive motility and are unable to fertilize zona-intact eggs. Here, analyses of sAC(-/-) spermatozoa provide additional insights into the functions linked to cAMP signalin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.05.038
更新日期:2006-08-15 00:00:00
abstract::Using DiI fluorescent dextrans, we have created fate maps of the neural plate and early neural tube describing the extent of progenitor cell dispersal and the spatial origin of morphologically distinct neuronal cell types along the dorsoventral axis of the developing chick spinal cord. Nonuniform dispersal and mixing ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8912
更新日期:1998-07-01 00:00:00
abstract::In vertebrates, hematopoietic and vascular progenitors develop from ventral mesoderm. The first primitive wave of hematopoiesis yields embryonic red blood cells, whereas progenitor cells of subsequent definitive waves form all hematopoietic cell lineages. In this report we examine the development of hematopoietic and ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8887
更新日期:1998-05-15 00:00:00
abstract::The Notch signaling pathway controls cell fate choices at multiple steps during cell lineage progression. To produce the cell fate choice appropriate for a particular stage in the cell lineage, Notch signaling needs to interpret the cell context information for each stage and convert it into the appropriate cell fate ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.09.018
更新日期:2010-12-15 00:00:00
abstract::Despite differences in the formation of neural precursors, all arthropod species analyzed so far generate about 30 single precursors (insects/crustaceans) or precursor groups (chelicerates/myriapods) per hemi-segment. In Drosophila, each precursor has a distinct identity conferred by segment polarity and dorso-ventral...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.11.029
更新日期:2006-02-15 00:00:00
abstract::The development of intrinsic ganglia, comprised of neurons and glia cells that innervate airway smooth muscle, is a recognized component of the growing lung. However, the embryological origin of these neurons and glia is unclear. The lung buds develop as an outgrowth of the foregut, which contains migrating neural cre...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.09.006
更新日期:2005-01-01 00:00:00
abstract::The protein content of various size follicles was measured in Fundulus heteroclitus and indicated four phases of increase relative to follicle volume: Phases I (previtellogenic; estimated to be less than 0.01 mg/mm3), II (vitellogenic; 0.20 mg/mm3), III (early maturation; 0.03 mg/mm3), and IV (late maturation; 0 mg/mm...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90106-x
更新日期:1985-08-01 00:00:00
abstract::Alkaline phosphatase (AP) activity is expressed in the endodermal cell lineage of ascidian embryos beginning at gastrulation. AP expression is resistant to levels of actinomycin D which completely suppress the appearance of other tissue-specific enzyme and morphological markers including acetylcholinesterase (AchE), a...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90043-1
更新日期:1987-02-01 00:00:00