Distinct enhancers at the Pax3 locus can function redundantly to regulate neural tube and neural crest expressions.

Abstract:

:Pax3 is a transcription factor expressed in somitic mesoderm, dorsal neural tube and pre-migratory neural crest during embryonic development. We have previously identified cis-acting enhancer elements within the proximal upstream genomic region of Pax3 that are sufficient to direct functional expression of Pax3 in neural crest. These elements direct expression of a reporter gene to pre-migratory neural crest in transgenic mice, and transgenic expression of a Pax3 cDNA using these elements is sufficient to rescue neural crest development in mice otherwise lacking endogenous Pax3. We show here that deletion of these enhancer sequences by homologous recombination is insufficient to abrogate neural crest expression of Pax3 and results in viable mice. We identify a distinct enhancer in the fourth intron that is also capable of mediating neural crest expression in transgenic mice and zebrafish. Our analysis suggests the existence of functionally redundant neural crest enhancer modules for Pax3.

journal_name

Dev Biol

journal_title

Developmental biology

authors

Degenhardt KR,Milewski RC,Padmanabhan A,Miller M,Singh MK,Lang D,Engleka KA,Wu M,Li J,Zhou D,Antonucci N,Li L,Epstein JA

doi

10.1016/j.ydbio.2009.12.030

subject

Has Abstract

pub_date

2010-03-15 00:00:00

pages

519-27

issue

2

eissn

0012-1606

issn

1095-564X

pii

S0012-1606(09)01455-9

journal_volume

339

pub_type

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